FastQCFastQC Report
Sat 18 Jun 2016
SRR3551135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820364
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112921.3764621558235124No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.4185946726087444No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC25020.30498656693857845No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.2771940260664778No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG20680.2520832215943167No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC17040.2077126738862261No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.19917987625005487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA302.1647393E-645.00000427
CGAATAT700.045.014
CGTTATT12150.043.148151
CGTTTTT47400.043.101271
CGTTTTA5300.042.02831
CGGTTTT12500.041.761
ACGGGTA603.6379788E-1241.2500045
ATTAGCG451.9272193E-840.01
CGCACGG403.4566074E-739.3752
CACGACC356.246104E-638.57142627
TACGACG356.246104E-638.5714261
TTAACGG700.038.5714262
GTTATTT14250.038.0526352
TTACGGG3150.037.8571433
CTAGACG301.13963695E-437.5000041
TCGTCAC301.13963695E-437.50000427
TATTACG301.13963695E-437.5000041
TACGTGC301.13963695E-437.50000416
AAGGGCG2100.037.55
TTTACGG850.037.0588232