##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592914
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821695895188846	31.0	31.0	34.0	30.0	34.0
2	31.919630840223036	33.0	31.0	34.0	30.0	34.0
3	31.594237950191765	33.0	31.0	34.0	30.0	34.0
4	35.47978458933336	37.0	35.0	37.0	33.0	37.0
5	30.95978000182151	37.0	35.0	37.0	0.0	37.0
6	33.27678381687732	37.0	35.0	37.0	17.0	37.0
7	29.71447123866193	35.0	32.0	37.0	0.0	37.0
8	32.67625827691706	35.0	33.0	37.0	17.0	37.0
9	36.52187838371164	39.0	35.0	39.0	32.0	39.0
10	36.88341142222987	38.0	37.0	39.0	33.0	39.0
11	37.05099559126619	39.0	37.0	39.0	34.0	39.0
12	37.1366201506458	39.0	37.0	39.0	34.0	39.0
13	37.017643368178184	39.0	37.0	39.0	33.0	39.0
14	38.336380992859	40.0	38.0	41.0	34.0	41.0
15	38.38353791612274	40.0	38.0	41.0	34.0	41.0
16	38.41032426287792	40.0	38.0	41.0	34.0	41.0
17	38.36414218588193	40.0	38.0	41.0	34.0	41.0
18	38.22564655245112	40.0	37.0	41.0	34.0	41.0
19	38.08195792307147	40.0	37.0	41.0	34.0	41.0
20	37.972103880158	40.0	37.0	41.0	34.0	41.0
21	37.89907305275301	40.0	36.0	41.0	33.0	41.0
22	37.89224744229349	40.0	36.0	41.0	33.0	41.0
23	37.84013195842905	40.0	36.0	41.0	33.0	41.0
24	37.77176791237852	40.0	36.0	41.0	33.0	41.0
25	37.655105124857904	39.0	36.0	41.0	33.0	41.0
26	37.497891768452085	39.0	36.0	41.0	33.0	41.0
27	37.468531692623216	39.0	36.0	41.0	33.0	41.0
28	37.37099478170527	39.0	36.0	41.0	33.0	41.0
29	37.345493612901706	39.0	36.0	41.0	33.0	41.0
30	37.188610827202595	39.0	35.0	41.0	32.0	41.0
31	37.07261930060683	39.0	35.0	41.0	31.0	41.0
32	36.91359286506981	39.0	35.0	41.0	31.0	41.0
33	36.621719170065134	39.0	35.0	41.0	30.0	41.0
34	36.39011053879652	39.0	35.0	41.0	30.0	41.0
35	36.14529425852653	39.0	35.0	41.0	29.0	41.0
36	35.98103097582449	39.0	35.0	41.0	27.0	41.0
37	35.81660578093956	39.0	35.0	40.0	26.0	41.0
38	35.685478838415015	39.0	35.0	40.0	26.0	41.0
39	35.61368259140448	39.0	35.0	40.0	25.0	41.0
40	35.45438461564409	39.0	35.0	40.0	24.0	41.0
41	35.39574542007779	39.0	35.0	40.0	24.0	41.0
42	35.354931743895406	39.0	35.0	40.0	24.0	41.0
43	35.223411827010324	39.0	35.0	40.0	24.0	41.0
44	35.09010412977261	38.0	34.0	40.0	23.0	41.0
45	34.97505034456936	38.0	34.0	40.0	23.0	41.0
46	34.83476018444496	38.0	34.0	40.0	23.0	41.0
47	34.67539643186027	38.0	34.0	40.0	22.0	41.0
48	34.57787638679471	38.0	34.0	40.0	22.0	41.0
49	34.42401933501317	38.0	33.0	40.0	21.0	41.0
50	34.26896986746813	38.0	33.0	40.0	20.0	41.0
51	32.30123086990693	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	19.0
10	35.0
11	19.0
12	18.0
13	18.0
14	11.0
15	24.0
16	56.0
17	105.0
18	258.0
19	462.0
20	808.0
21	1268.0
22	2180.0
23	3362.0
24	5119.0
25	7402.0
26	9395.0
27	10126.0
28	10406.0
29	10950.0
30	12729.0
31	15344.0
32	19316.0
33	25923.0
34	36029.0
35	44822.0
36	56802.0
37	89816.0
38	116087.0
39	113984.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.61263522197148	20.838772570726952	24.48179668552269	14.066795521778875
2	33.08203213282196	26.076294369841158	24.976303477401444	15.865370019935437
3	30.771241697784163	25.8983596271972	28.40951638854876	14.920882286469874
4	26.597280549961717	25.366410643027486	30.86450986146389	17.17179894554691
5	22.015840408558407	38.92554400806863	23.65418930907349	15.404426274299476
6	23.999433307359922	35.64564169508563	27.102244170318123	13.252680827236327
7	67.81421926282732	18.465409823347063	10.296265562965287	3.424105350860327
8	81.15797569293355	6.002556863221311	10.017978998640613	2.821488445204532
9	75.06653578765217	8.369173269647874	11.126234158748149	5.438056783951804
10	32.988426652094574	38.75621085014016	17.111925169586144	11.143437328179129
11	25.752470004081534	27.099545633936795	30.97481253605076	16.17317182593091
12	25.370458447599482	24.65652691621382	32.436913279160215	17.53610135702648
13	23.34065311326769	24.70240203469643	33.709273182957396	18.247671669078482
14	19.34344609842237	29.74832775073619	31.016639849961376	19.89158630088006
15	19.047113072047548	28.565019547522912	34.9588641860371	17.429003194392443
16	22.2494324640673	28.651541370249312	31.49225688717082	17.606769278512566
17	22.822871445099963	26.459823853037705	31.32832080200501	19.388983899857315
18	22.5359832960598	28.12751933669976	30.611690734238017	18.724806633002427
19	22.91883814516102	28.79810562746031	29.406456922926427	18.87659930445225
20	24.59311131125256	28.42081650964558	30.00468870696256	16.9813834721393
21	23.79080271337833	28.069332145977327	30.261218321712764	17.878646818931582
22	23.192908246389866	25.11696468627828	30.771241697784163	20.91888536954769
23	21.95141285245415	27.972859470344773	31.476908961502005	18.59881871569907
24	21.552535443588784	26.790731876798326	31.488377741122658	20.168354938490236
25	21.44054618376358	29.716619948255563	29.227004253567973	19.61582961441288
26	21.98160272822028	29.584728982618053	29.098823775454786	19.334844513706877
27	22.060534917374188	28.96575220015044	29.754736774641856	19.218976107833512
28	19.906428250977378	28.63636210310433	31.881183443130034	19.576026202788263
29	22.94143838735466	26.860725164189077	30.20421174065716	19.993624707799107
30	21.655417143126996	28.336318589205177	30.160866500032046	19.84739776763578
31	22.48167525138553	28.07877702331198	28.449319800173377	20.99022792512911
32	24.092026836944314	29.02292743972988	28.352678466016997	18.532367257308817
33	21.64850214364984	29.182478403276026	28.801816114984636	20.367203338089503
34	22.024779310321566	28.008615077397398	30.18363540074952	19.78297021153152
35	21.880576272444234	29.099498409550122	28.45134370245938	20.568581615546268
36	21.401080089186628	30.057310166398498	27.602822669054873	20.93878707536
37	21.77752591438219	29.95628371062245	28.66031161348863	19.60587876150673
38	20.975554633555625	29.66450446439113	27.519505358281304	21.840435543771946
39	22.629757435311024	27.765915461601516	28.982112076962256	20.622215026125204
40	22.27456258411844	28.55405674347376	30.05039516692134	19.120985505486463
41	20.378672117710156	28.979582199104758	29.557068984709417	21.084676698475665
42	21.749022623854387	28.955126713148953	28.81514013836745	20.480710524629202
43	22.606145241974385	27.851931308756413	27.949921911103466	21.592001538165736
44	22.327690019125875	28.18536921037452	28.003049346110902	21.483891424388695
45	21.990372971459603	27.335330250255517	27.743315219407872	22.930981558877004
46	21.738059819805233	29.610196419716857	27.629302057296673	21.022441703181237
47	21.636358729933853	27.161443312183554	30.26003771204593	20.942160245836664
48	21.90132127087571	27.387951709691453	28.378145903115797	22.332581116317037
49	20.893923908020387	27.17358672589954	29.66686568372479	22.265623682355283
50	20.303281757556746	27.736231561406882	29.45216338288521	22.508323298151165
51	20.916018174642527	27.142722216038077	28.121953605413264	23.81930600390613
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	413.5
2	647.0
3	4663.5
4	8680.0
5	6932.0
6	5184.0
7	4261.0
8	3338.0
9	3228.5
10	3119.0
11	3061.0
12	3003.0
13	2887.0
14	2771.0
15	2754.5
16	2738.0
17	2632.5
18	2527.0
19	2598.0
20	2669.0
21	2788.0
22	2907.0
23	3125.0
24	3343.0
25	4104.0
26	5674.0
27	6483.0
28	8272.0
29	10061.0
30	11866.5
31	13672.0
32	15543.0
33	17414.0
34	19431.0
35	21448.0
36	22934.5
37	24421.0
38	26536.0
39	28651.0
40	32125.5
41	35600.0
42	37175.0
43	38750.0
44	42022.0
45	45294.0
46	50678.0
47	56062.0
48	52595.0
49	49128.0
50	46218.5
51	43309.0
52	39238.5
53	35168.0
54	32074.0
55	28980.0
56	25965.0
57	22950.0
58	21391.0
59	19832.0
60	18593.0
61	17354.0
62	15121.0
63	12888.0
64	10137.5
65	7387.0
66	5901.5
67	4416.0
68	3631.5
69	2847.0
70	2327.0
71	1807.0
72	1462.0
73	1117.0
74	1002.0
75	696.5
76	506.0
77	362.0
78	218.0
79	165.0
80	112.0
81	90.0
82	68.0
83	54.5
84	41.0
85	37.5
86	34.0
87	30.5
88	27.0
89	16.0
90	5.0
91	3.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.959053239537916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7080597156317	30.190123420755988
2	8.816208080883706	7.222070723115204
3	4.194540061112355	5.154131690354269
4	2.671313874002584	4.376579487391525
5	1.830383254514387	3.748538258520673
6	1.4060665629123696	3.4554693125197113
7	1.0577195237116956	3.032623319894422
8	0.8291065078758505	2.716753407786746
9	0.692909205420143	2.5542814531472597
>10	4.731260516510714	32.705666706897965
>50	0.04318974274938911	1.1496819824675637
>100	0.016249606170103622	1.4814153262435752
>500	8.552424300054538E-4	0.23417501669279497
>1k	0.0021381060750136345	1.9784898942122753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3929	0.6626593401403914	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTC	2315	0.39044448267370985	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCG	2066	0.348448510239259	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGC	1728	0.291441929183659	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1258	0.21217242298208508	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.12902377073234905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.37317047666272E-4	0.0	0.0	0.051609508292939615	0.0
2	3.37317047666272E-4	0.0	0.0	0.2568669317978661	0.0
3	3.37317047666272E-4	0.0	0.0	0.36463972852724	0.0
4	3.37317047666272E-4	0.0	0.0	0.5973884914169677	0.0
5	3.37317047666272E-4	0.0	0.0	1.4776173273021045	0.0
6	3.37317047666272E-4	0.0	0.0	1.9189966841734214	0.0
7	3.37317047666272E-4	0.0	0.0	2.34216092047076	0.0
8	3.37317047666272E-4	0.0	0.0	2.988797700847003	0.0
9	3.37317047666272E-4	0.0	0.0	3.2731559720296706	0.0
10	3.37317047666272E-4	0.0	0.0	4.305852113459962	0.0
11	3.37317047666272E-4	0.0	0.0	4.838813048772672	0.0
12	3.37317047666272E-4	0.0	0.0	5.698296886226333	0.0
13	3.37317047666272E-4	0.0	0.0	5.927672478639398	0.0
14	3.37317047666272E-4	0.0	0.0	6.044890152703427	0.0
15	3.37317047666272E-4	0.0	0.0	6.235474284634871	0.0
16	3.37317047666272E-4	0.0	0.0	6.527253530866196	0.0
17	3.37317047666272E-4	0.0	0.0	6.904373990157088	0.0
18	5.05975571499408E-4	0.0	0.0	7.313876886023943	0.0
19	5.05975571499408E-4	0.0	0.0	7.584911133823792	0.0
20	5.05975571499408E-4	0.0	0.0	7.845994528717487	0.0
21	5.05975571499408E-4	0.0	0.0	8.16779499219111	0.0
22	5.05975571499408E-4	0.0	0.0	8.54204825657684	0.0
23	5.05975571499408E-4	0.0	0.0	8.908205911818577	0.0
24	5.05975571499408E-4	0.0	0.0	9.205382230812564	0.0
25	5.05975571499408E-4	0.0	0.0	9.467308918325424	0.0
26	5.05975571499408E-4	0.0	0.0	9.722657923408791	0.0
27	5.05975571499408E-4	0.0	0.0	9.983404001254819	0.0
28	5.05975571499408E-4	0.0	0.0	10.268099589485153	0.0
29	5.05975571499408E-4	0.0	0.0	10.5701670056703	0.0
30	5.05975571499408E-4	0.0	0.0	10.919458808528724	0.0
31	5.05975571499408E-4	0.0	0.0	11.236874150382686	0.0
32	5.05975571499408E-4	0.0	0.0	11.542652054092162	0.0
33	5.05975571499408E-4	0.0	0.0	11.85399568908813	0.0
34	5.05975571499408E-4	0.0	0.0	12.18793956627774	0.0
35	5.05975571499408E-4	0.0	0.0	12.515643078085523	0.0
36	5.05975571499408E-4	0.0	0.0	12.840142077940477	0.0
37	5.05975571499408E-4	0.0	0.0	13.173579979558587	0.0
38	5.05975571499408E-4	0.0	0.0	13.527256904036673	0.0
39	5.05975571499408E-4	0.0	0.0	13.912304313947722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	20	7.03052E-4	45.0	27
ACCGAGC	20	7.03052E-4	45.0	17
ATTCCCG	20	7.03052E-4	45.0	30
TCGCTAA	20	7.03052E-4	45.0	12
CCGGTGA	20	7.03052E-4	45.0	43
TCGACGT	20	7.03052E-4	45.0	26
TGTAACG	25	3.8885126E-5	45.0	1
CACCCGT	20	7.03052E-4	45.0	16
GCATACG	20	7.03052E-4	45.0	1
CGGTTTT	640	0.0	42.890625	1
CGTTATT	470	0.0	42.606384	1
CGTTTTA	180	0.0	42.500004	1
CGTTTTT	2060	0.0	42.160194	1
CGAATAT	75	0.0	42.0	14
GTAACGG	60	3.6379788E-12	41.250004	2
TACGGCT	280	0.0	40.98214	7
CGGTTTA	55	6.002665E-11	40.909092	1
TGCACGG	90	0.0	40.000004	2
CTACGAA	80	0.0	39.375	11
ATAGGCG	35	6.2431736E-6	38.571426	5
>>END_MODULE