##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390510
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.000678599779775	33.0	31.0	34.0	30.0	34.0
2	32.14136641827354	33.0	31.0	34.0	30.0	34.0
3	32.0705231620189	33.0	31.0	34.0	30.0	34.0
4	35.78108371104453	37.0	35.0	37.0	35.0	37.0
5	31.3807252055005	37.0	35.0	37.0	0.0	37.0
6	33.54139970807406	37.0	35.0	37.0	17.0	37.0
7	29.406089472740774	35.0	32.0	37.0	0.0	37.0
8	32.52317994417557	35.0	33.0	37.0	17.0	37.0
9	36.44662620675527	39.0	35.0	39.0	32.0	39.0
10	36.905853883383266	38.0	37.0	39.0	34.0	39.0
11	37.042313897211336	39.0	37.0	39.0	34.0	39.0
12	37.06275639548283	39.0	37.0	39.0	34.0	39.0
13	36.85071060920335	39.0	37.0	39.0	33.0	39.0
14	38.13872884177102	40.0	37.0	41.0	33.0	41.0
15	38.21679598473791	40.0	37.0	41.0	33.0	41.0
16	38.269716524544826	40.0	37.0	41.0	34.0	41.0
17	38.257701467311975	40.0	37.0	41.0	34.0	41.0
18	38.162633479296304	40.0	37.0	41.0	33.0	41.0
19	38.096158869171084	40.0	37.0	41.0	34.0	41.0
20	38.03500294486697	40.0	37.0	41.0	34.0	41.0
21	37.947284320503954	40.0	37.0	41.0	33.0	41.0
22	37.933371744641626	40.0	36.0	41.0	33.0	41.0
23	37.89432280863486	40.0	36.0	41.0	33.0	41.0
24	37.8332309031779	40.0	36.0	41.0	33.0	41.0
25	37.70110624567873	40.0	36.0	41.0	33.0	41.0
26	37.565770402806585	40.0	36.0	41.0	33.0	41.0
27	37.52814012445264	40.0	36.0	41.0	33.0	41.0
28	37.40282195078231	40.0	36.0	41.0	32.0	41.0
29	37.387598268930375	40.0	36.0	41.0	32.0	41.0
30	37.30054287982382	39.0	36.0	41.0	32.0	41.0
31	37.18202862922845	39.0	35.0	41.0	32.0	41.0
32	37.091377941666025	39.0	35.0	41.0	31.0	41.0
33	36.93640367724258	39.0	35.0	41.0	31.0	41.0
34	36.774169675552486	39.0	35.0	41.0	31.0	41.0
35	36.705520985378094	39.0	35.0	41.0	30.0	41.0
36	36.59881181019692	39.0	35.0	41.0	30.0	41.0
37	36.483175846969345	39.0	35.0	41.0	30.0	41.0
38	36.420352871885484	39.0	35.0	41.0	30.0	41.0
39	36.380914701288056	39.0	35.0	41.0	30.0	41.0
40	36.20787431819928	39.0	35.0	40.0	30.0	41.0
41	36.114253156129166	39.0	35.0	40.0	29.0	41.0
42	36.06886123274692	39.0	35.0	40.0	29.0	41.0
43	35.94299249699112	39.0	35.0	40.0	29.0	41.0
44	35.788870963611686	38.0	35.0	40.0	28.0	41.0
45	35.67067680725206	38.0	35.0	40.0	28.0	41.0
46	35.57649484008092	38.0	34.0	40.0	27.0	41.0
47	35.42995057745	38.0	34.0	40.0	27.0	41.0
48	35.35535069524468	38.0	34.0	40.0	27.0	41.0
49	35.20454277739366	38.0	34.0	40.0	26.0	41.0
50	35.026601111367185	38.0	34.0	40.0	26.0	41.0
51	33.08550613300556	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	20.0
10	18.0
11	21.0
12	13.0
13	14.0
14	15.0
15	30.0
16	49.0
17	94.0
18	163.0
19	259.0
20	450.0
21	684.0
22	1062.0
23	1582.0
24	2156.0
25	2848.0
26	3690.0
27	4380.0
28	5168.0
29	6145.0
30	7756.0
31	9818.0
32	13164.0
33	18682.0
34	27578.0
35	33387.0
36	38357.0
37	60840.0
38	77312.0
39	74739.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.478579293744076	20.851194591687793	25.695116642339507	11.975109472228624
2	30.66963714117436	26.561163606565774	26.26616475890502	16.503034493354843
3	32.67317098153696	24.459808967760107	27.037207753962768	15.829812296740162
4	29.56492791477811	26.382679060715475	25.95964251875752	18.092750505748892
5	22.475992932319276	40.08373665207037	20.64633428081227	16.793936134798084
6	26.001126731709817	36.19343934854421	23.330516504058796	14.474917415687177
7	69.79462753834729	20.15748636398556	6.072827840516248	3.9750582571509057
8	83.93075721492409	6.198816931704694	6.720186422882897	3.1502394304883357
9	77.21466799825869	9.314998335509975	8.206447978284807	5.263885687946532
10	41.53337942690328	31.099331643235768	14.353025530716243	13.014263399144706
11	31.226345035978593	26.86742977132468	24.012189188497093	17.894036004199638
12	31.483444726127374	23.417582136180894	26.539909349312435	18.559063788379298
13	25.97705564518194	25.90253770710097	27.35218048193388	20.768226165783208
14	20.54031906993419	29.452766894573763	27.56907633607334	22.437837699418708
15	20.95695372717728	28.465852346930937	31.399708074057003	19.17748585183478
16	22.94333051650406	27.310952344370182	30.508053570971295	19.237663568154463
17	22.9597193413741	26.687408773142813	27.669713963790937	22.683157921692146
18	23.449847635143787	28.19569281196384	27.27612609152135	21.078333461371027
19	25.793961742336947	28.951371283705924	25.219072494942512	20.03559447901462
20	27.306599062764075	27.981869862487518	26.515326112007376	18.19620496274103
21	25.999334203989655	27.289442011728255	26.766792143607077	19.944431640675013
22	24.139714732017108	26.45566054646488	25.92635271824025	23.478272003277763
23	25.33712324908453	27.183938951627358	26.71864997055133	20.760287828736782
24	24.113595042380474	26.48306061304448	27.306855138152674	22.09648920642237
25	23.314383754577346	28.2522854728432	25.978336022124914	22.454994750454535
26	22.024019871450154	30.492432972267036	25.819057130419193	21.664490025863614
27	22.642185859517042	29.864023968656372	26.513021433510026	20.98076873831656
28	21.16744769660188	29.560574633172003	28.4264167370874	20.845560933138714
29	23.10670661442729	28.127576758597733	27.809787201352076	20.955929425622905
30	23.251133133594532	28.049729840465034	26.725820081431973	21.973316944508465
31	24.224219610253257	28.092494430360297	25.399093493124376	22.284192466262066
32	25.698957773168416	29.037668689662237	25.575785511254512	19.687588025914827
33	24.888223092878544	29.834063148190825	24.398863025274643	20.878850733655987
34	21.99098614632148	28.513994519986685	27.70991779980026	21.785101533891577
35	23.999385419067373	27.54423702337968	26.223656244398352	22.232721313154595
36	23.439348544211416	29.67862538731402	25.628280965916368	21.253745102558195
37	23.82704668254334	30.24660059921641	26.034160456838496	19.892192261401757
38	23.851373844459808	28.368031548487878	25.321758725768866	22.458835881283452
39	24.815497682517734	26.40342116719162	26.814166090497043	21.966915059793603
40	23.82115694860567	27.213387621315714	28.4719981562572	20.493457273821413
41	21.06988297354741	28.128857135540702	28.97953957645131	21.821720314460578
42	22.332334639317818	28.173414253156132	26.670251722106986	22.823999385419068
43	24.576579344959157	27.225935315356843	25.975263117461783	22.22222222222222
44	23.017848454585028	28.447158843563546	25.610099613326163	22.924893088525263
45	22.851911602775857	28.372384830093978	25.64646231850657	23.129241248623593
46	22.11902384061868	29.815113569434843	25.591662185347364	22.474200404599117
47	22.460116258226424	27.665872832962023	28.179560062482395	21.694450846329158
48	22.916698676090242	26.090497042329265	27.909912678292486	23.082891603288008
49	22.103915392691608	26.90353640111649	27.87098921922614	23.121558986965763
50	20.2699034595785	27.348083275716373	28.703746382935137	23.678266881769993
51	21.545158894778623	26.334536887659727	27.075106911474737	25.045197306086912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	480.0
2	762.0
3	1279.5
4	1797.0
5	1444.5
6	1092.0
7	930.5
8	769.0
9	771.0
10	773.0
11	764.0
12	755.0
13	757.0
14	759.0
15	786.0
16	813.0
17	830.5
18	848.0
19	985.5
20	1123.0
21	1393.5
22	1664.0
23	1794.5
24	1925.0
25	2444.5
26	3503.5
27	4043.0
28	5389.0
29	6735.0
30	7905.5
31	9076.0
32	10035.5
33	10995.0
34	11978.5
35	12962.0
36	13332.0
37	13702.0
38	15362.0
39	17022.0
40	18941.0
41	20860.0
42	21762.5
43	22665.0
44	24821.0
45	26977.0
46	28764.0
47	30551.0
48	29931.5
49	29312.0
50	28386.0
51	27460.0
52	25523.5
53	23587.0
54	22370.5
55	21154.0
56	20290.5
57	19427.0
58	19251.5
59	19076.0
60	18898.5
61	18721.0
62	16346.5
63	13972.0
64	11744.0
65	9516.0
66	7843.5
67	6171.0
68	4967.0
69	3763.0
70	3226.5
71	2690.0
72	2162.0
73	1634.0
74	1296.0
75	749.5
76	541.0
77	423.0
78	305.0
79	248.0
80	191.0
81	141.0
82	91.0
83	71.5
84	52.0
85	38.5
86	25.0
87	18.0
88	11.0
89	9.5
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	5.5
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.36488842569341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.68495065221143	25.704503438433136
2	9.06362112380906	6.5919514180014875
3	4.648537557337452	5.071306488456654
4	2.989461544871339	4.348457421285892
5	2.064080187712361	3.753002286392216
6	1.558122821395426	3.3996577532142767
7	1.1123646689708728	2.8315711953066423
8	0.8929139905117712	2.597657411096098
9	0.7728396508305688	2.529380490606494
>10	6.136334186449263	39.86633042697452
>50	0.05564326290034356	1.366220408198073
>100	0.01831297260011307	1.2986009504244902
>500	0.0028173804000173955	0.641360311610044
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	727	0.1861668075081304	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	671	0.17182658574684387	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	592	0.1515966300478861	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	514	0.13162274973752272	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	494	0.12650124196563467	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	446	0.11420962331310337	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.560753885944022E-4	0.0	0.0	0.04481319300402038	0.0
2	2.560753885944022E-4	0.0	0.0	0.20562853704130496	0.0
3	2.560753885944022E-4	0.0	0.0	0.28680443522573046	0.0
4	2.560753885944022E-4	0.0	0.0	0.43737676371923895	0.0
5	2.560753885944022E-4	0.0	0.0	0.9272489821003304	0.0
6	2.560753885944022E-4	0.0	0.0	1.201505723284935	0.0
7	2.560753885944022E-4	0.0	0.0	1.4831886507387775	0.0
8	2.560753885944022E-4	0.0	0.0	1.9523187626437224	0.0
9	2.560753885944022E-4	0.0	0.0	2.139253796317636	0.0
10	2.560753885944022E-4	0.0	0.0	2.7469206934521524	0.0
11	2.560753885944022E-4	0.0	0.0	3.349466082814781	0.0
12	2.560753885944022E-4	0.0	0.0	3.9809479910885766	0.0
13	2.560753885944022E-4	0.0	0.0	4.196051317507874	0.0
14	2.560753885944022E-4	0.0	0.0	4.298225397557041	0.0
15	2.560753885944022E-4	0.0	0.0	4.502317482266779	0.0
16	2.560753885944022E-4	0.0	0.0	4.858006197024404	0.0
17	2.560753885944022E-4	0.0	0.0	5.272336175770147	0.0
18	2.560753885944022E-4	0.0	0.0	5.760928017208266	0.0
19	2.560753885944022E-4	0.0	0.0	6.038769813833192	0.0
20	2.560753885944022E-4	0.0	0.0	6.3396583954316155	0.0
21	2.560753885944022E-4	0.0	0.0	6.6746050037130935	0.0
22	2.560753885944022E-4	0.0	0.0	7.062047066656423	0.0
23	2.560753885944022E-4	0.0	0.0	7.449489129599754	0.0
24	2.560753885944022E-4	0.0	0.0	7.758828199021792	0.0
25	2.560753885944022E-4	0.0	0.0	8.02335407543981	0.0
26	2.560753885944022E-4	0.0	0.0	8.264321016107141	0.0
27	2.560753885944022E-4	0.0	0.0	8.508873012214796	0.0
28	2.560753885944022E-4	0.0	0.0	8.781081150290646	0.0
29	2.560753885944022E-4	0.0	0.0	9.074287470231235	0.0
30	2.560753885944022E-4	0.0	0.0	9.399247138357532	0.0
31	2.560753885944022E-4	0.0	0.0	9.70858620777957	0.0
32	2.560753885944022E-4	0.0	0.0	10.015876674092853	0.0
33	2.560753885944022E-4	0.0	0.0	10.296023149215129	0.0
34	2.560753885944022E-4	0.0	0.0	10.59793603236793	0.0
35	2.560753885944022E-4	0.0	0.0	10.89344703080587	0.0
36	2.560753885944022E-4	0.0	0.0	11.222503905149676	0.0
37	2.560753885944022E-4	0.0	0.0	11.540805613172518	0.0
38	2.560753885944022E-4	0.0	0.0	11.833755857724514	0.0
39	2.560753885944022E-4	0.0	0.0	12.157691224296434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.027559E-4	45.000004	39
CAACGTG	20	7.027559E-4	45.000004	18
CAGCACG	20	7.027559E-4	45.000004	1
AACGGGC	40	6.7957444E-9	45.000004	4
GTCGTTT	20	7.027559E-4	45.000004	9
GCTAGCG	20	7.027559E-4	45.000004	1
CTTTCGT	20	7.027559E-4	45.000004	15
CAATGCG	20	7.027559E-4	45.000004	1
CCAACGG	20	7.027559E-4	45.000004	2
TTCGCAG	20	7.027559E-4	45.000004	1
TCGAATA	20	7.027559E-4	45.000004	14
TGACTAC	20	7.027559E-4	45.000004	4
TCGTTCC	25	3.886059E-5	45.0	38
CGTTCCA	25	3.886059E-5	45.0	39
TTGCGGA	25	3.886059E-5	45.0	1
GCTAGTA	25	3.886059E-5	45.0	12
TCCGAAC	30	2.161807E-6	44.999996	35
CGTTGAT	65	0.0	41.53846	25
ACGGGTC	55	6.002665E-11	40.909092	5
CGGGGTA	55	6.002665E-11	40.909092	6
>>END_MODULE