FastQCFastQC Report
Sat 18 Jun 2016
SRR3551131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613941
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25230.410951540946117No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG14750.24025109904697683No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC11480.18698865200401993No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC10780.17558690493060408No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.1299799166369407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG253.888674E-545.0000041
TTAAACG253.888674E-545.0000041
ATACGCA253.888674E-545.00000417
TAGCGAA207.0307154E-445.01
GACTTCG207.0307154E-445.041
TTGCGTC207.0307154E-445.043
CGATGTA406.8066583E-945.010
ATGTTCG207.0307154E-445.038
AATACCG207.0307154E-445.01
TGATTCG302.1638443E-644.99999615
TAATCGT302.1638443E-644.99999621
CGGCGCA603.6379788E-1241.24999622
CTACGGG1050.040.7142873
CGTTTTA1450.040.3448261
TTTGACG451.925764E-840.0000041
TAGTACG451.925764E-840.0000041
CGTTTTT10350.039.565221
GGTACTC403.4547156E-739.3759
TTTCGTC403.4547156E-739.37516
CCCCGAT403.4547156E-739.37540