##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551131_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	613941
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810032234367796	31.0	31.0	34.0	30.0	34.0
2	31.936697500248396	33.0	31.0	34.0	30.0	34.0
3	31.764909005914248	33.0	31.0	34.0	30.0	34.0
4	35.55771157163311	37.0	35.0	37.0	33.0	37.0
5	31.14863969013309	37.0	35.0	37.0	0.0	37.0
6	33.359251133252215	37.0	35.0	37.0	17.0	37.0
7	29.575946222845516	35.0	32.0	37.0	0.0	37.0
8	32.61131607108826	35.0	33.0	37.0	17.0	37.0
9	36.458736262930806	39.0	35.0	39.0	32.0	39.0
10	36.780550574077964	38.0	35.0	39.0	33.0	39.0
11	36.935184325529654	39.0	37.0	39.0	33.0	39.0
12	37.01777043722442	39.0	37.0	39.0	33.0	39.0
13	36.87516064247216	39.0	37.0	39.0	33.0	39.0
14	38.13520028797556	40.0	37.0	41.0	33.0	41.0
15	38.19863960869204	40.0	37.0	41.0	33.0	41.0
16	38.24002306410551	40.0	37.0	41.0	33.0	41.0
17	38.17131287859908	40.0	37.0	41.0	33.0	41.0
18	38.07794397181488	40.0	37.0	41.0	33.0	41.0
19	37.969480780726485	40.0	37.0	41.0	33.0	41.0
20	37.889528472605676	40.0	36.0	41.0	33.0	41.0
21	37.81462388079636	40.0	36.0	41.0	33.0	41.0
22	37.80394044378857	40.0	36.0	41.0	33.0	41.0
23	37.76495298408153	40.0	36.0	41.0	33.0	41.0
24	37.710589454035485	40.0	36.0	41.0	33.0	41.0
25	37.580804995919806	39.0	36.0	41.0	33.0	41.0
26	37.43049250660894	39.0	36.0	41.0	33.0	41.0
27	37.391653269613855	39.0	36.0	41.0	32.0	41.0
28	37.315988018392645	39.0	36.0	41.0	32.0	41.0
29	37.2495256058807	39.0	36.0	41.0	32.0	41.0
30	37.12481003874965	39.0	35.0	41.0	32.0	41.0
31	37.008051262254845	39.0	35.0	41.0	31.0	41.0
32	36.83902361953347	39.0	35.0	41.0	31.0	41.0
33	36.661627094460215	39.0	35.0	41.0	30.0	41.0
34	36.404266859519076	39.0	35.0	41.0	30.0	41.0
35	36.27409148436087	39.0	35.0	41.0	30.0	41.0
36	36.054456372843646	39.0	35.0	40.0	29.0	41.0
37	35.92399595400861	39.0	35.0	40.0	28.0	41.0
38	35.787966596138716	39.0	35.0	40.0	27.0	41.0
39	35.65010318581101	39.0	35.0	40.0	26.0	41.0
40	35.53225961452322	38.0	35.0	40.0	26.0	41.0
41	35.29830553750279	38.0	34.0	40.0	25.0	41.0
42	35.278301986672986	38.0	34.0	40.0	25.0	41.0
43	35.074402914938084	38.0	34.0	40.0	24.0	41.0
44	34.94995610327377	38.0	34.0	40.0	24.0	41.0
45	34.80435905078827	38.0	34.0	40.0	23.0	41.0
46	34.62014428096511	38.0	34.0	40.0	23.0	41.0
47	34.442045734036334	38.0	33.0	40.0	22.0	41.0
48	34.32550521955693	38.0	33.0	40.0	22.0	41.0
49	34.126530073736724	38.0	33.0	40.0	21.0	41.0
50	33.95863283279664	37.0	33.0	40.0	20.0	41.0
51	31.804482841185067	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	19.0
10	29.0
11	24.0
12	21.0
13	14.0
14	25.0
15	29.0
16	61.0
17	109.0
18	228.0
19	455.0
20	796.0
21	1306.0
22	2092.0
23	3274.0
24	4647.0
25	6570.0
26	8522.0
27	9808.0
28	10893.0
29	12349.0
30	14460.0
31	17590.0
32	22366.0
33	30477.0
34	41912.0
35	50303.0
36	59383.0
37	91574.0
38	114165.0
39	110413.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.802033094385294	21.665762671005844	25.647741395345808	12.884462839263055
2	31.260984361689477	25.92366367452247	26.00233572932904	16.81301623445901
3	30.94059526892649	25.191508630308125	28.203361560801444	15.664534539963936
4	27.217273321052023	25.234672387086054	29.43996247196392	18.108091819898004
5	21.683028173717016	38.92377280553017	22.710651349233885	16.682547671518925
6	24.444694196999386	34.947006308423774	26.181994686785863	14.426304807790977
7	68.32496933744447	18.88617961660811	8.63226270928314	4.156588336664272
8	82.108867138699	5.104399282667227	8.892059660455972	3.8946739181778054
9	75.70401716125816	7.557892370765269	10.348225643832224	6.38986482414434
10	37.37818454867813	31.839541584614807	17.621400101964195	13.160873764742867
11	27.23551611636949	25.45505186980508	29.192381678369745	18.117050335455687
12	27.214015679031046	22.52692033925084	31.208536325151766	19.05052765656635
13	22.868646987251218	24.22854964890763	32.391223260867086	20.511580102974065
14	18.51220231260007	28.41299082485125	30.93131098916671	22.14349587338197
15	18.2442612563748	27.33194232019038	35.30306658131644	19.12072984211838
16	21.461182752088558	26.654841426130528	32.213681770723895	19.670294051057024
17	20.617616350756833	26.603044917997003	30.49055202372866	22.288786707517495
18	21.154149991611572	28.208248023832912	29.951249387156093	20.686352597399424
19	22.351659198522334	28.442309603040034	28.83013188563722	20.375899312800417
20	23.747558804510533	28.127621383813757	29.44761793071321	18.677201880962503
21	22.660646544211904	28.57668733640529	29.551373829081296	19.21129229030151
22	21.625693674147843	25.68927633111325	29.06565940375378	23.619370590985127
23	21.77896573123476	27.426902585101825	30.094585636079035	20.699546047584377
24	21.712835598208947	25.411399466724	30.514169928380742	22.36159500668631
25	20.83082902102971	28.8597764280281	28.139837541392414	22.169557009549777
26	20.00941458544062	28.74592183939499	28.879485162255	22.365178412909383
27	20.943217670753377	28.798044111730608	29.21469652621343	21.044041691302585
28	18.92429402825353	28.114753697830896	30.673631505307515	22.28732076860806
29	20.22621066193657	25.66989336108844	31.729270402204772	22.374625574770214
30	20.522167439542237	26.276792069596265	30.17912144652336	23.021919044338137
31	21.80323516429103	26.758597324498606	27.609982066680676	23.828185444529687
32	22.366481469717776	27.153749301642993	29.04253014540485	21.43723908323438
33	21.144539947649694	26.27288289917109	29.956950260692807	22.625626892486412
34	20.90640631591635	25.983604287708427	30.951019723393614	22.158969672981605
35	21.58920808351291	24.566692890684934	30.219679089684515	23.62441993611764
36	21.060981429811658	26.78237811125173	30.017705284384004	22.138935174552604
37	21.641167473747476	26.77065059997622	30.403572981768605	21.184608944507698
38	20.448870494070277	26.691978545169647	29.957276024894902	22.901874935865173
39	22.73019720135974	24.667191147032046	31.213585670284278	21.38902598132394
40	23.2641247285977	24.62663350387089	31.737251625156166	20.371990142375246
41	20.805256531165046	26.319304297970003	30.556682156754476	22.318757014110478
42	21.419159170017966	25.709310829542254	30.00174283848122	22.86978716195856
43	21.39114344863757	25.578679384501118	30.046209652067546	22.983967514793765
44	21.37094606810752	25.748076769591865	29.337509630404224	23.543467531896386
45	21.417530349007478	24.848478925499357	29.238965959269702	24.495024766223462
46	21.6245534994405	26.795082915133538	28.59704759903639	22.98331598638957
47	21.401893667306794	24.931060150731096	31.4950459408966	22.172000241065508
48	21.084599334463736	24.314551398261397	30.742367751950106	23.858481515324762
49	20.038896245730452	24.391757514158527	32.03825774789434	23.53108849221668
50	19.148908445599822	24.38605664062182	32.03809486579329	24.426940047985067
51	19.78708051750901	24.12446798633745	30.167882581550998	25.920568914602544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	535.5
2	902.0
3	3350.5
4	5799.0
5	4530.0
6	3261.0
7	2669.0
8	2077.0
9	1976.5
10	1876.0
11	1871.5
12	1867.0
13	1789.5
14	1712.0
15	1762.5
16	1813.0
17	1776.0
18	1739.0
19	2031.5
20	2324.0
21	2578.0
22	2832.0
23	3140.0
24	3448.0
25	4356.5
26	6648.5
27	8032.0
28	9701.5
29	11371.0
30	13073.5
31	14776.0
32	16597.5
33	18419.0
34	19993.0
35	21567.0
36	23092.5
37	24618.0
38	26021.5
39	27425.0
40	30019.5
41	32614.0
42	35240.0
43	37866.0
44	42136.0
45	46406.0
46	52096.5
47	57787.0
48	55523.5
49	53260.0
50	49913.0
51	46566.0
52	40938.5
53	35311.0
54	31161.5
55	27012.0
56	25514.0
57	24016.0
58	23451.0
59	22886.0
60	22204.0
61	21522.0
62	19184.0
63	16846.0
64	13685.5
65	10525.0
66	8734.5
67	6944.0
68	5856.5
69	4769.0
70	3904.0
71	3039.0
72	2572.5
73	2106.0
74	1781.5
75	1078.5
76	700.0
77	540.5
78	381.0
79	313.5
80	246.0
81	214.5
82	183.0
83	130.5
84	78.0
85	67.5
86	57.0
87	47.0
88	37.0
89	24.0
90	11.0
91	10.5
92	10.0
93	8.0
94	6.0
95	5.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	613941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.756215249698094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.96038758953378	32.13574509888766
2	8.343268157922333	6.967666021763142
3	3.4101569035682138	4.271857371019148
4	2.081570398403559	3.4767400645255533
5	1.441487826524514	3.0095537982088536
6	1.0523956378199257	2.6366435268391286
7	0.8341146599023267	2.438062989726719
8	0.6679842465508298	2.2313995185907083
9	0.5411642660895822	2.0337274422253344
>10	4.59286237440476	36.446719562420675
>50	0.05484691755268631	1.4835778901960912
>100	0.017341304781230537	1.600950622038386
>500	8.065723154060717E-4	0.21925294097969694
>1k	0.0016131446308121434	1.0481031525788278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2523	0.410951540946117	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG	1475	0.24025109904697683	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	1148	0.18698865200401993	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	1078	0.17558690493060408	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	798	0.1299799166369407	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12639651041386712	0.0
2	0.0	0.0	0.0	0.5130786183037133	0.0
3	0.0	0.0	0.0	0.7490947827234213	0.0
4	0.0	0.0	0.0	1.0691581112843092	0.0
5	0.0	0.0	0.0	2.149555087540985	0.0
6	0.0	0.0	0.0	2.8431070738067663	0.0
7	0.0	0.0	0.0	3.50522281457013	0.0
8	0.0	0.0	0.0	4.586271319231001	0.0
9	0.0	0.0	0.0	5.0604211153840515	0.0
10	0.0	0.0	0.0	6.406967444754463	0.0
11	0.0	0.0	0.0	7.546979269994999	0.0
12	0.0	0.0	0.0	8.691063147761756	0.0
13	0.0	0.0	0.0	9.102177570808921	0.0
14	0.0	0.0	0.0	9.291120808025527	0.0
15	0.0	0.0	0.0	9.57258107863785	0.0
16	0.0	0.0	0.0	10.100807732339101	0.0
17	0.0	0.0	0.0	10.761131769990927	0.0
18	0.0	0.0	0.0	11.47471825468571	0.0
19	0.0	0.0	0.0	11.928182024005563	0.0
20	0.0	0.0	0.0	12.36372876221005	0.0
21	0.0	0.0	0.0	12.910361093329815	0.0
22	0.0	0.0	0.0	13.522634911172247	0.0
23	0.0	0.0	0.0	14.160481218879339	0.0
24	0.0	0.0	0.0	14.629744552000925	0.0
25	0.0	0.0	0.0	15.03548386571348	0.0
26	0.0	0.0	0.0	15.415650689561375	0.0
27	0.0	0.0	0.0	15.82008694646554	0.0
28	0.0	0.0	0.0	16.23999700296934	0.0
29	0.0	0.0	0.0	16.667236754020337	0.0
30	0.0	0.0	0.0	17.150019301528975	0.0
31	0.0	0.0	0.0	17.58410010082402	0.0
32	0.0	0.0	0.0	18.020624131634797	0.0
33	0.0	0.0	0.0	18.427503620054697	0.0
34	0.0	0.0	0.0	18.818583544672858	0.0
35	0.0	0.0	0.0	19.247126352532245	0.0
36	0.0	0.0	0.0	19.682184444433585	0.0
37	0.0	0.0	0.0	20.111215898596118	0.0
38	0.0	0.0	0.0	20.547902811507946	0.0
39	1.6288210104879785E-4	0.0	0.0	21.021889725559948	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	25	3.888674E-5	45.000004	1
TTAAACG	25	3.888674E-5	45.000004	1
ATACGCA	25	3.888674E-5	45.000004	17
TAGCGAA	20	7.0307154E-4	45.0	1
GACTTCG	20	7.0307154E-4	45.0	41
TTGCGTC	20	7.0307154E-4	45.0	43
CGATGTA	40	6.8066583E-9	45.0	10
ATGTTCG	20	7.0307154E-4	45.0	38
AATACCG	20	7.0307154E-4	45.0	1
TGATTCG	30	2.1638443E-6	44.999996	15
TAATCGT	30	2.1638443E-6	44.999996	21
CGGCGCA	60	3.6379788E-12	41.249996	22
CTACGGG	105	0.0	40.714287	3
CGTTTTA	145	0.0	40.344826	1
TTTGACG	45	1.925764E-8	40.000004	1
TAGTACG	45	1.925764E-8	40.000004	1
CGTTTTT	1035	0.0	39.56522	1
GGTACTC	40	3.4547156E-7	39.375	9
TTTCGTC	40	3.4547156E-7	39.375	16
CCCCGAT	40	3.4547156E-7	39.375	40
>>END_MODULE