FastQCFastQC Report
Sat 18 Jun 2016
SRR3551129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348231
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG4760.1366908747354486No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3880.11142029285158415No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC3820.10969729863222975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCA207.026505E-445.0000049
TTTACGT207.026505E-445.00000411
TCCGACG207.026505E-445.00000429
TGAACGG406.7921064E-945.0000042
GGATACG207.026505E-445.0000041
TGTGACA207.026505E-445.00000442
ACGATGG302.1611268E-645.0000042
ATATCGA302.1611268E-645.00000418
CCATACC207.026505E-445.00000420
TTACGTT207.026505E-445.00000412
CGAGATC207.026505E-445.00000415
ATGCGAG207.026505E-445.0000041
ACGTATG207.026505E-445.0000041
ACCCGGC302.1611268E-645.00000441
TATCGAG207.026505E-445.00000419
GTAGTAC207.026505E-445.00000438
TGCGTAG302.1611268E-645.0000041
TTGGCCG253.885187E-545.01
CCCGGCT351.2086639E-745.042
ACGGGTG603.6379788E-1241.2500045