##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348231
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.918407608742474	31.0	31.0	34.0	30.0	34.0
2	32.05227277295818	33.0	31.0	34.0	30.0	34.0
3	31.99485973391226	33.0	31.0	34.0	30.0	34.0
4	35.71035031344137	37.0	35.0	37.0	35.0	37.0
5	31.08750226142991	37.0	35.0	37.0	0.0	37.0
6	33.34914754860998	37.0	35.0	37.0	17.0	37.0
7	29.614353690510036	35.0	32.0	37.0	0.0	37.0
8	32.617805422262805	35.0	33.0	37.0	17.0	37.0
9	36.44500920366079	39.0	35.0	39.0	32.0	39.0
10	36.829848577524686	38.0	37.0	39.0	33.0	39.0
11	36.9718778626831	39.0	37.0	39.0	33.0	39.0
12	36.94140383825679	39.0	35.0	39.0	33.0	39.0
13	36.69770927918537	39.0	35.0	39.0	33.0	39.0
14	37.95030884671382	40.0	37.0	41.0	33.0	41.0
15	38.03821026847122	40.0	37.0	41.0	33.0	41.0
16	38.11073109516384	40.0	37.0	41.0	33.0	41.0
17	38.070789217502174	40.0	37.0	41.0	33.0	41.0
18	37.99088823223665	40.0	37.0	41.0	33.0	41.0
19	37.92111845298092	40.0	36.0	41.0	33.0	41.0
20	37.848663674400036	40.0	36.0	41.0	33.0	41.0
21	37.758350060735545	40.0	36.0	41.0	33.0	41.0
22	37.75349121703697	40.0	36.0	41.0	33.0	41.0
23	37.7241428821673	40.0	36.0	41.0	33.0	41.0
24	37.65178573992551	40.0	36.0	41.0	33.0	41.0
25	37.52034138258799	39.0	36.0	41.0	33.0	41.0
26	37.34958978379294	39.0	35.0	41.0	32.0	41.0
27	37.315066722951144	39.0	35.0	41.0	32.0	41.0
28	37.213194689731814	39.0	35.0	41.0	32.0	41.0
29	37.188512797539566	39.0	35.0	41.0	32.0	41.0
30	37.031734681863476	39.0	35.0	41.0	31.0	41.0
31	36.922241270880534	39.0	35.0	41.0	31.0	41.0
32	36.816159388451915	39.0	35.0	41.0	31.0	41.0
33	36.62634573027674	39.0	35.0	41.0	30.0	41.0
34	36.480040547797294	39.0	35.0	41.0	30.0	41.0
35	36.363115288414875	39.0	35.0	41.0	30.0	41.0
36	36.25553727267245	39.0	35.0	40.0	30.0	41.0
37	36.12890868417803	39.0	35.0	40.0	29.0	41.0
38	36.0642389678116	39.0	35.0	40.0	29.0	41.0
39	36.07323874095069	39.0	35.0	40.0	29.0	41.0
40	35.89685295105835	39.0	35.0	40.0	28.0	41.0
41	35.77942802335232	38.0	35.0	40.0	28.0	41.0
42	35.708707725618915	38.0	35.0	40.0	27.0	41.0
43	35.516590998503865	38.0	34.0	40.0	27.0	41.0
44	35.35953146043862	38.0	34.0	40.0	26.0	41.0
45	35.23206721974781	38.0	34.0	40.0	26.0	41.0
46	35.08489479684462	38.0	34.0	40.0	25.0	41.0
47	34.915090844870214	38.0	34.0	40.0	24.0	41.0
48	34.83044301053037	38.0	34.0	40.0	24.0	41.0
49	34.64082462503338	38.0	34.0	40.0	24.0	41.0
50	34.498984869239095	37.0	33.0	40.0	23.0	41.0
51	32.44201119371911	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	21.0
10	23.0
11	15.0
12	11.0
13	18.0
14	18.0
15	30.0
16	41.0
17	95.0
18	164.0
19	294.0
20	509.0
21	842.0
22	1163.0
23	1710.0
24	2322.0
25	3124.0
26	3830.0
27	4516.0
28	5341.0
29	6229.0
30	7681.0
31	9549.0
32	12736.0
33	17800.0
34	25476.0
35	30611.0
36	33361.0
37	52086.0
38	65576.0
39	63019.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.623718164092224	20.712687842265623	26.05425708796747	10.609336905674681
2	32.27857370538521	26.04535495116747	24.35251313065178	17.32355821279553
3	33.560481404584884	24.79532264502586	25.508355086135353	16.1358408642539
4	29.745485037231035	25.80786891459979	25.870758203606226	18.57588784456295
5	22.580987907452236	40.42862352863473	20.12887996760771	16.861508596305328
6	26.409481062857697	35.68522044275208	23.89505816541319	14.01024032897703
7	69.37119325964662	19.62949880969816	6.794340538320827	4.204967392334399
8	81.63144579316604	6.692396713675692	7.565667617185144	4.1104898759731325
9	75.07200680008386	8.987425013855745	9.773110377881348	6.167457808179053
10	40.154380282054156	29.321341293566626	16.429037047247373	14.095241377131845
11	32.489353331552906	26.30725007250934	23.94473783207124	17.258658763866514
12	32.69553830646898	22.656799653103832	26.477826500225426	18.169835540201763
13	25.426225695012793	25.815048057180434	27.44442625728324	21.314299990523534
14	20.28337511594315	29.348334869669845	27.83181279093475	22.53647722345225
15	19.97122599653678	27.958165700354076	33.216169726417235	18.85443857669191
16	22.902039163658607	25.60197110538694	30.892137690211385	20.60385204074307
17	22.70188466851027	26.038750139993283	27.07771565426398	24.18164953723247
18	23.663315442910022	27.57221499521869	27.802521889205728	20.96194767266556
19	26.160508398160992	28.464726000844266	25.18960115555479	20.185164445439952
20	27.139456280457512	26.745752101335036	27.592029428741267	18.52276218946619
21	25.509790914651482	27.860529361257324	26.120879531115836	20.50880019297535
22	24.202612633567945	25.981029833644907	26.48902596265123	23.327331570135915
23	24.140872007374416	27.273275498160704	27.34276959834133	21.24308289612355
24	23.556776967013278	24.98226751782581	29.571175455373016	21.8897800597879
25	22.65076917333609	27.450743902754205	26.15591374690938	23.742573177000324
26	21.324063624433208	29.370159463115	27.031769141747862	22.27400777070393
27	21.52507961669122	29.567729466934306	27.600644399838036	21.306546516536436
28	19.512909534188513	29.412947152895637	29.227438108611814	21.84670520430404
29	22.72198626773607	27.22359583150265	27.787876438341215	22.26654146242006
30	22.247014194600713	28.864460659734487	26.896514095528545	21.99201105013626
31	24.19227466825182	28.23097311841852	25.467864721980526	22.108887491349133
32	25.33749149271604	29.078399108637658	25.00466644267742	20.579442955968883
33	23.856865126884166	29.081845097076364	25.88856247720622	21.172727298833248
34	22.746682518213486	27.664969517360603	27.820613328508948	21.767734635916963
35	23.17771823875531	27.69856790463801	26.85717239418662	22.26654146242006
36	23.950481146135754	29.912328310805187	25.300734282703147	20.836456260355913
37	23.329628895761722	30.632252728792096	26.30523991258676	19.732878462859425
38	21.94146988636853	29.781667915837474	25.295565300045087	22.98129689774891
39	23.924923398548664	26.87842265622532	27.050434912457533	22.146219032768478
40	23.659295123064865	26.500512590780257	28.011578521154064	21.828613765000817
41	21.437206911504145	27.822910654134752	27.79821440365734	22.941668030703756
42	22.324261768768434	27.451031068457432	26.267046874057737	23.957660288716397
43	22.72141193632962	27.420591503915503	26.474380511786716	23.383616047968157
44	21.786974738033088	27.90446571385086	26.431305656302857	23.877253891813194
45	22.427928587632923	28.203118045205628	25.829119176638493	23.539834190522956
46	22.34120454525875	30.017718123889026	25.071288885825787	22.569788445026433
47	21.896097705258867	27.05474239800592	28.37455597003139	22.674603926703828
48	22.20049335067814	25.762209567786897	29.002874528689286	23.03442255284567
49	21.364841154291263	26.74115745008342	28.20771269645724	23.68628869916808
50	20.26356068242058	27.203781397980077	28.045176908431475	24.487481011167876
51	20.087528106343203	26.307537238212564	27.397331081954217	26.20760357349001
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	176.0
1	421.5
2	667.0
3	947.5
4	1228.0
5	1027.0
6	826.0
7	695.5
8	565.0
9	594.5
10	624.0
11	668.0
12	712.0
13	722.5
14	733.0
15	820.5
16	908.0
17	925.0
18	942.0
19	1062.5
20	1183.0
21	1218.5
22	1254.0
23	1483.0
24	1712.0
25	2212.5
26	3103.5
27	3494.0
28	4296.0
29	5098.0
30	6189.5
31	7281.0
32	8301.5
33	9322.0
34	10236.5
35	11151.0
36	11960.5
37	12770.0
38	13998.5
39	15227.0
40	16796.5
41	18366.0
42	19747.0
43	21128.0
44	23047.0
45	24966.0
46	26981.5
47	28997.0
48	28138.5
49	27280.0
50	26348.5
51	25417.0
52	23311.5
53	21206.0
54	19989.0
55	18772.0
56	17663.5
57	16555.0
58	16622.5
59	16690.0
60	16108.0
61	15526.0
62	13701.0
63	11876.0
64	10242.0
65	8608.0
66	6969.0
67	5330.0
68	4298.0
69	3266.0
70	2641.5
71	2017.0
72	1687.5
73	1358.0
74	1158.0
75	730.0
76	502.0
77	418.0
78	334.0
79	259.5
80	185.0
81	149.0
82	113.0
83	114.0
84	115.0
85	74.5
86	34.0
87	23.5
88	13.0
89	14.5
90	16.0
91	13.0
92	10.0
93	8.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	348231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.876363419948554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.96376755460092	26.906384088321143
2	8.77834827908264	6.474271227326629
3	4.017072500028162	4.444050761859594
4	2.579961006411407	3.805583187268931
5	1.8753964986758798	3.457890142083541
6	1.3614182379946593	3.0122492226502247
7	1.0000007047943775	2.5813472587141515
8	0.7611761323262101	2.2455526145801743
9	0.6538555409024975	2.1700633095420785
>10	5.915708161001425	41.50149153228308
>50	0.07152646120607363	1.7910120328836863
>100	0.02176892297576154	1.6101046224867588
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	476	0.1366908747354486	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	388	0.11142029285158415	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	382	0.10969729863222975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06375078611611258	0.0
2	0.0	0.0	0.0	0.3311020558192694	0.0
3	0.0	0.0	0.0	0.4471169999224653	0.0
4	0.0	0.0	0.0	0.6260212330320966	0.0
5	0.0	0.0	0.0	1.2732927281028972	0.0
6	0.0	0.0	0.0	1.620188897599582	0.0
7	0.0	0.0	0.0	2.015041739534964	0.0
8	0.0	0.0	0.0	2.679256011096083	0.0
9	0.0	0.0	0.0	2.932248995637953	0.0
10	0.0	0.0	0.0	3.717647193960331	0.0
11	0.0	0.0	0.0	4.438433109056919	0.0
12	0.0	0.0	0.0	5.169556989469633	0.0
13	0.0	0.0	0.0	5.4308777794050505	0.0
14	0.0	0.0	0.0	5.534257432566314	0.0
15	0.0	0.0	0.0	5.761979835224319	0.0
16	0.0	0.0	0.0	6.2484385364887105	0.0
17	0.0	0.0	0.0	6.755286002682127	0.0
18	0.0	0.0	0.0	7.348283179843265	0.0
19	0.0	0.0	0.0	7.688861703868984	0.0
20	0.0	0.0	0.0	8.045234341572117	0.0
21	0.0	0.0	0.0	8.496371661339744	0.0
22	0.0	0.0	0.0	8.95210363235898	0.0
23	0.0	0.0	0.0	9.37739603883629	0.0
24	0.0	0.0	0.0	9.75616760139103	0.0
25	0.0	0.0	0.0	10.088705485726429	0.0
26	0.0	0.0	0.0	10.402577599352155	0.0
27	0.0	0.0	0.0	10.706398913364978	0.0
28	0.0	0.0	0.0	11.041808454732635	0.0
29	0.0	0.0	0.0	11.366592865080937	0.0
30	0.0	0.0	0.0	11.741631273493745	0.0
31	0.0	0.0	0.0	12.108054710809778	0.0
32	0.0	0.0	0.0	12.459258365854849	0.0
33	0.0	0.0	0.0	12.802134215506374	0.0
34	0.0	0.0	0.0	13.14012824820306	0.0
35	0.0	0.0	0.0	13.552498198035213	0.0
36	0.0	0.0	0.0	13.921506126680278	0.0
37	0.0	0.0	0.0	14.28132475282212	0.0
38	0.0	0.0	0.0	14.671582943505891	0.0
39	0.0	0.0	0.0	15.067871613957402	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGCA	20	7.026505E-4	45.000004	9
TTTACGT	20	7.026505E-4	45.000004	11
TCCGACG	20	7.026505E-4	45.000004	29
TGAACGG	40	6.7921064E-9	45.000004	2
GGATACG	20	7.026505E-4	45.000004	1
TGTGACA	20	7.026505E-4	45.000004	42
ACGATGG	30	2.1611268E-6	45.000004	2
ATATCGA	30	2.1611268E-6	45.000004	18
CCATACC	20	7.026505E-4	45.000004	20
TTACGTT	20	7.026505E-4	45.000004	12
CGAGATC	20	7.026505E-4	45.000004	15
ATGCGAG	20	7.026505E-4	45.000004	1
ACGTATG	20	7.026505E-4	45.000004	1
ACCCGGC	30	2.1611268E-6	45.000004	41
TATCGAG	20	7.026505E-4	45.000004	19
GTAGTAC	20	7.026505E-4	45.000004	38
TGCGTAG	30	2.1611268E-6	45.000004	1
TTGGCCG	25	3.885187E-5	45.0	1
CCCGGCT	35	1.2086639E-7	45.0	42
ACGGGTG	60	3.6379788E-12	41.250004	5
>>END_MODULE