##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428126
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.809189350798597	31.0	31.0	34.0	30.0	34.0
2	31.954868426584696	33.0	31.0	34.0	30.0	34.0
3	31.912429985564998	33.0	31.0	34.0	30.0	34.0
4	35.64281076131793	37.0	35.0	37.0	33.0	37.0
5	31.15083176448055	37.0	35.0	37.0	0.0	37.0
6	33.3616809070227	37.0	35.0	37.0	17.0	37.0
7	29.532826784638168	35.0	32.0	37.0	0.0	37.0
8	32.557270523163744	35.0	33.0	37.0	17.0	37.0
9	36.35238457837179	39.0	35.0	39.0	32.0	39.0
10	36.720619630669475	38.0	35.0	39.0	33.0	39.0
11	36.89922359305438	39.0	37.0	39.0	33.0	39.0
12	36.918622087890014	39.0	37.0	39.0	33.0	39.0
13	36.72873873579273	39.0	35.0	39.0	33.0	39.0
14	37.93806262642306	40.0	37.0	41.0	33.0	41.0
15	38.02784460649435	40.0	37.0	41.0	33.0	41.0
16	38.06710407683719	40.0	37.0	41.0	33.0	41.0
17	38.02828606531722	40.0	37.0	41.0	33.0	41.0
18	37.963274830306965	40.0	37.0	41.0	33.0	41.0
19	37.87520963454684	40.0	37.0	41.0	33.0	41.0
20	37.82516128429481	40.0	36.0	41.0	33.0	41.0
21	37.71278782414523	40.0	36.0	41.0	33.0	41.0
22	37.67691987872729	39.0	36.0	41.0	33.0	41.0
23	37.626257223340794	39.0	36.0	41.0	33.0	41.0
24	37.569960712500524	39.0	36.0	41.0	33.0	41.0
25	37.4321041001948	39.0	36.0	41.0	32.0	41.0
26	37.21623307157239	39.0	35.0	41.0	32.0	41.0
27	37.16622209349584	39.0	35.0	41.0	32.0	41.0
28	36.986765578357776	39.0	35.0	41.0	31.0	41.0
29	36.985410836996586	39.0	35.0	41.0	31.0	41.0
30	36.865177541191144	39.0	35.0	41.0	31.0	41.0
31	36.73374427154623	39.0	35.0	41.0	30.0	41.0
32	36.66246151833806	39.0	35.0	40.0	30.0	41.0
33	36.4797279305625	39.0	35.0	40.0	30.0	41.0
34	36.36791505304513	39.0	35.0	40.0	30.0	41.0
35	36.30317009478518	39.0	35.0	40.0	30.0	41.0
36	36.16008838519501	39.0	35.0	40.0	29.0	41.0
37	36.05772833231338	39.0	35.0	40.0	29.0	41.0
38	35.94176714331762	38.0	35.0	40.0	29.0	41.0
39	35.9243774029141	38.0	35.0	40.0	29.0	41.0
40	35.765842298762514	38.0	35.0	40.0	28.0	41.0
41	35.63167385302458	38.0	35.0	40.0	27.0	41.0
42	35.57829237187183	38.0	35.0	40.0	27.0	41.0
43	35.38970069558962	38.0	34.0	40.0	26.0	41.0
44	35.22835099947212	38.0	34.0	40.0	26.0	41.0
45	35.1094841238327	38.0	34.0	40.0	25.0	41.0
46	34.9378080284776	38.0	34.0	40.0	24.0	41.0
47	34.783554374179566	38.0	33.0	40.0	24.0	41.0
48	34.70554462938481	38.0	33.0	40.0	24.0	41.0
49	34.47850866333743	37.0	33.0	40.0	23.0	41.0
50	34.29279698032822	37.0	33.0	40.0	23.0	41.0
51	32.052535935682485	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	12.0
10	26.0
11	15.0
12	4.0
13	6.0
14	18.0
15	25.0
16	41.0
17	95.0
18	199.0
19	391.0
20	636.0
21	1114.0
22	1523.0
23	2163.0
24	2905.0
25	3842.0
26	4888.0
27	5665.0
28	6740.0
29	8127.0
30	9997.0
31	12796.0
32	16366.0
33	22142.0
34	31366.0
35	37435.0
36	44097.0
37	65606.0
38	78997.0
39	70869.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.3416891289013	22.351130274732206	26.378449335008852	11.928731261357637
2	30.22568122468619	26.19976362099008	25.911530717592484	17.66302443673124
3	32.06532656274088	24.580380542176837	26.588434245993003	16.76585864908929
4	29.784455977913044	25.351181661473493	25.591998617229507	19.27236374338396
5	21.487599445023196	39.9095126201165	20.882637354423697	17.7202505804366
6	25.44484567627287	34.84067774440235	24.577811205112514	15.136665374212265
7	68.43779634967277	20.0071941437801	6.831867254032692	4.723142252514447
8	80.90819057940887	6.305153155846643	7.837412350569692	4.949243914174799
9	74.33629352106624	9.117409360795653	10.094691749625111	6.451605368513008
10	38.616668924568934	30.600570859980476	17.073244792420923	13.70951542302967
11	31.070525966654678	26.227559176504112	24.59766517333682	18.10424968350439
12	29.140953831348714	23.27095294375955	28.600925895647546	18.987167329244194
13	24.212031037591736	26.20887308876359	29.427318125972263	20.151777747672416
14	18.929941185538837	29.695463485048794	27.84951159238168	23.525083737030688
15	18.932510522603156	27.972139043178878	32.94987924115797	20.14547119305999
16	21.300738567617945	26.17757389179821	32.10783741235057	20.41385012823328
17	20.30990876517661	27.32723544003401	27.959525933954023	24.403329860835363
18	22.35486749228031	27.108141061276353	28.18189037806627	22.355101068377067
19	23.982425734480035	28.273452207994843	25.84426080172659	21.899861255798527
20	26.23923798134194	27.282622405553504	26.344580800979152	20.1335588121254
21	23.856995370521762	28.91158210433377	26.640988867763227	20.590433657381237
22	22.484268649883447	26.412551445135314	26.10329669302962	24.99988321195162
23	22.45530521388563	27.8429714616725	27.853482386026545	21.848240938415326
24	21.62097139627119	25.536407506201446	29.305858555658848	23.536762541868516
25	21.65764284346197	27.996898109435076	26.73114924111126	23.61430980599169
26	21.196096476271002	28.588779938616206	26.495470959483892	23.719652625628903
27	22.85355245885557	27.948080705212952	26.952345804739725	22.246021031191752
28	19.397326955148717	28.501422478429244	28.762326978506326	23.33892358791571
29	20.90809714897016	26.89301747616356	27.989423674338866	24.209461700527417
30	21.736124411972177	26.365369073590482	28.365014037923412	23.533492476513924
31	22.351597426925718	26.640288139472958	25.87672787917576	25.131386554425568
32	22.480765008432098	26.846302256812248	26.971499044673763	23.701433690081892
33	21.172972442692103	27.447293553766883	26.333602724431593	25.046131279109424
34	19.40106417269682	26.471646197614724	29.322208882431806	24.80508074725665
35	20.670083106375227	24.337928553743524	28.42107230114499	26.570916038736257
36	20.382784507364654	29.14889541863844	25.792640484343394	24.675679589653512
37	20.422726019910026	28.10948178807174	28.47269261852819	22.995099573490048
38	19.208597468969415	28.545101208522727	26.39690184665262	25.84939947585524
39	22.331977034798168	25.39883118521183	27.706329445069912	24.562862334920094
40	22.379159406342993	23.754689040142388	29.90077687409781	23.965374679416808
41	18.64708053236664	26.655003433568623	27.846708679220605	26.851207354844135
42	21.107103983406756	26.490565861452005	27.18942554294764	25.212904612193604
43	21.778868837678626	26.374244965267234	27.273279361683244	24.573606835370896
44	21.123921462373225	26.33079981127051	26.698448587565345	25.846830138790917
45	21.566781741823668	24.940554883375455	26.767587112205288	26.725076262595593
46	21.081644188860288	26.321690343497007	25.824406833502287	26.772258634140417
47	20.244974610278284	25.069021736591562	30.423286602542245	24.262717050587913
48	20.393529007815456	24.401694828158067	29.32664682827018	25.878129335756295
49	20.24941255611666	24.615650532787075	29.96617818118965	25.168758729906614
50	18.102848226923847	25.266627114447616	30.250440290942386	26.380084367686145
51	19.53700546100914	23.38026655704162	27.999701022596152	29.083026959353088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	280.0
1	532.5
2	785.0
3	1145.5
4	1506.0
5	1222.5
6	939.0
7	795.0
8	651.0
9	686.0
10	721.0
11	719.5
12	718.0
13	746.0
14	774.0
15	808.0
16	842.0
17	995.0
18	1148.0
19	1057.5
20	967.0
21	1236.5
22	1506.0
23	1616.5
24	1727.0
25	2516.0
26	4024.5
27	4744.0
28	6186.5
29	7629.0
30	8383.0
31	9137.0
32	9815.0
33	10493.0
34	11341.5
35	12190.0
36	12674.0
37	13158.0
38	15006.5
39	16855.0
40	19112.5
41	21370.0
42	23381.0
43	25392.0
44	28544.5
45	31697.0
46	34566.5
47	37436.0
48	38621.0
49	39806.0
50	37837.0
51	35868.0
52	32140.0
53	28412.0
54	25362.5
55	22313.0
56	21314.5
57	20316.0
58	19297.0
59	18278.0
60	17850.0
61	17422.0
62	15007.5
63	12593.0
64	10855.5
65	9118.0
66	7569.5
67	6021.0
68	5196.0
69	4371.0
70	3636.5
71	2902.0
72	2465.0
73	2028.0
74	1635.0
75	956.5
76	671.0
77	507.5
78	344.0
79	255.5
80	167.0
81	144.5
82	122.0
83	107.5
84	93.0
85	62.5
86	32.0
87	28.5
88	25.0
89	15.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	428126.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.345769650671414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65060274161696	28.978396501047698
2	9.571721033299022	7.532134618733396
3	4.159425976081933	4.9096744900181655
4	2.482990945942429	3.9078075921501405
5	1.7322542443998112	3.4078438238276414
6	1.2057055262079597	2.8463647140431965
7	0.8739821511893681	2.4071250279646605
8	0.6968858889182636	2.1935609326545107
9	0.5651119956903281	2.001128976833658
>10	4.944433921933944	37.12154731111548
>50	0.09032067137449823	2.302960504434602
>100	0.025384240974532176	2.09345265017338
>500	0.0011806623709084735	0.29800285700348905
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	718	0.16770763747121176	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	565	0.13197049466745772	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	489	0.11421871131395897	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	441	0.10300705866964398	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	439	0.10253990647613084	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	435	0.1016056020891046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3357609675656233E-4	0.0	0.0	0.13056903808691833	0.0
2	2.3357609675656233E-4	0.0	0.0	0.584874546278432	0.0
3	2.3357609675656233E-4	0.0	0.0	0.8161148820674288	0.0
4	2.3357609675656233E-4	0.0	0.0	1.1533987657839047	0.0
5	2.3357609675656233E-4	0.0	0.0	2.204024048994922	0.0
6	2.3357609675656233E-4	0.0	0.0	2.9204019377472985	0.0
7	2.3357609675656233E-4	0.0	0.0	3.675553458561265	0.0
8	2.3357609675656233E-4	0.0	0.0	5.048747331393095	0.0
9	2.3357609675656233E-4	0.0	0.0	5.616103670414785	0.0
10	2.3357609675656233E-4	0.0	0.0	6.800334480970555	0.0
11	2.3357609675656233E-4	0.0	0.0	8.12027300374189	0.0
12	2.3357609675656233E-4	0.0	0.0	9.20126317953126	0.0
13	2.3357609675656233E-4	0.0	0.0	9.693641591494092	0.0
14	2.3357609675656233E-4	0.0	0.0	9.899889284930138	0.0
15	2.3357609675656233E-4	0.0	0.0	10.226662244292568	0.0
16	2.3357609675656233E-4	0.0	0.0	10.84143453095584	0.0
17	2.3357609675656233E-4	0.0	0.0	11.6106006175752	0.0
18	2.3357609675656233E-4	0.0	0.0	12.507065676926887	0.0
19	2.3357609675656233E-4	0.0	0.0	12.979823696762168	0.0
20	2.3357609675656233E-4	0.0	0.0	13.470333499950948	0.0
21	2.3357609675656233E-4	0.0	0.0	14.081134992969359	0.0
22	2.3357609675656233E-4	0.0	0.0	14.714593367373157	0.0
23	2.3357609675656233E-4	0.0	0.0	15.32586201258508	0.0
24	2.3357609675656233E-4	0.0	0.0	15.807729500193869	0.0
25	2.3357609675656233E-4	0.0	0.0	16.25946567132106	0.0
26	2.3357609675656233E-4	0.0	0.0	16.661216557742346	0.0
27	2.3357609675656233E-4	0.0	0.0	17.0599309549058	0.0
28	2.3357609675656233E-4	0.0	0.0	17.464718330584923	0.0
29	2.3357609675656233E-4	0.0	0.0	17.912250131970495	0.0
30	2.3357609675656233E-4	0.0	0.0	18.39692053274036	0.0
31	2.3357609675656233E-4	0.0	0.0	18.85169319312539	0.0
32	2.3357609675656233E-4	0.0	0.0	19.303195788155822	0.0
33	2.3357609675656233E-4	0.0	0.0	19.71779335989872	0.0
34	2.3357609675656233E-4	0.0	0.0	20.135427420899454	0.0
35	2.3357609675656233E-4	0.0	0.0	20.585995711542864	0.0
36	2.3357609675656233E-4	0.0	0.0	21.037965458766813	0.0
37	2.3357609675656233E-4	0.0	0.0	21.47428560750807	0.0
38	2.3357609675656233E-4	0.0	0.0	21.886313842186645	0.0
39	2.3357609675656233E-4	0.0	0.0	22.344122991829508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	30	2.1622982E-6	45.000004	31
TCGTTGA	45	3.8380676E-10	45.0	24
GTCGATA	20	7.0283207E-4	45.0	43
TTTACGG	55	1.8189894E-12	45.0	2
AGATACG	25	3.8866907E-5	45.0	1
TAGAACG	25	3.8866907E-5	45.0	1
CGTTGAT	45	3.8380676E-10	45.0	25
GCACGCT	20	7.0283207E-4	45.0	17
TACGCAA	20	7.0283207E-4	45.0	1
GTTAACG	25	3.8866907E-5	45.0	1
CGTAGCA	20	7.0283207E-4	45.0	14
CCTCATG	20	7.0283207E-4	45.0	28
TTAACGG	25	3.8866907E-5	45.0	2
GCAATTG	20	7.0283207E-4	45.0	1
CCAACGA	20	7.0283207E-4	45.0	17
CGATATC	25	3.8866907E-5	45.0	10
GTCGGTA	20	7.0283207E-4	45.0	23
TACGTAG	20	7.0283207E-4	45.0	1
GACTACG	20	7.0283207E-4	45.0	39
TCACGTC	25	3.8866907E-5	45.0	38
>>END_MODULE