FastQCFastQC Report
Sat 18 Jun 2016
SRR3551125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593149
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.1616794431078869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGTC351.210592E-745.016
CGCGACA207.030523E-445.021
ATCCGTA207.030523E-445.045
CCCCGTC253.8885148E-545.019
CGGATTC207.030523E-445.018
CGATAGT207.030523E-445.010
CCGTTAA207.030523E-445.030
CTTACGA207.030523E-445.038
ATTGCGG700.041.7857132
TAACGGG1650.040.909093
ACGGGTC403.4544428E-739.3755
CGCACGG403.4544428E-739.3752
ACGACCA1550.039.19354628
TAATGGG3250.038.769233
GATAACG356.2431754E-638.5714261
AATTGCG356.2431754E-638.5714261
TACGAAT1400.038.57142612
GCGAGAC1600.037.9687521
CGCATAG301.13925555E-437.5000042
CGATTCG301.13925555E-437.50000410