##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593149
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00128635469334	33.0	31.0	34.0	30.0	34.0
2	32.154730093113194	33.0	31.0	34.0	30.0	34.0
3	32.08737602187646	33.0	31.0	34.0	30.0	34.0
4	35.80057456052358	37.0	35.0	37.0	35.0	37.0
5	31.176586321480773	37.0	35.0	37.0	0.0	37.0
6	33.43946967793927	37.0	35.0	37.0	17.0	37.0
7	29.59386933131473	35.0	32.0	37.0	0.0	37.0
8	32.671662600796765	35.0	35.0	37.0	17.0	37.0
9	36.56231065044365	39.0	35.0	39.0	32.0	39.0
10	36.876445884592236	38.0	37.0	39.0	33.0	39.0
11	36.91851625814087	39.0	37.0	39.0	33.0	39.0
12	36.93185860551059	39.0	35.0	39.0	33.0	39.0
13	36.74271557399574	39.0	35.0	39.0	33.0	39.0
14	37.94754774938506	40.0	37.0	41.0	33.0	41.0
15	38.071283943831986	40.0	37.0	41.0	33.0	41.0
16	38.141190493451056	40.0	37.0	41.0	33.0	41.0
17	38.10629875461309	40.0	37.0	41.0	33.0	41.0
18	38.02439521941367	40.0	37.0	41.0	33.0	41.0
19	37.96619230581186	40.0	37.0	41.0	33.0	41.0
20	37.88957074866518	40.0	36.0	41.0	33.0	41.0
21	37.813053718374306	40.0	36.0	41.0	33.0	41.0
22	37.77946013564888	40.0	36.0	41.0	33.0	41.0
23	37.733443030334705	40.0	36.0	41.0	33.0	41.0
24	37.70628627882707	40.0	36.0	41.0	33.0	41.0
25	37.57758505872892	39.0	36.0	41.0	33.0	41.0
26	37.41873627031319	39.0	35.0	41.0	33.0	41.0
27	37.361909065007275	39.0	35.0	41.0	32.0	41.0
28	37.24504804020575	39.0	35.0	41.0	32.0	41.0
29	37.19312516753801	39.0	35.0	41.0	32.0	41.0
30	36.969412407337785	39.0	35.0	41.0	31.0	41.0
31	36.85226309072425	39.0	35.0	41.0	31.0	41.0
32	36.75069670521235	39.0	35.0	41.0	31.0	41.0
33	36.5329015137849	39.0	35.0	41.0	30.0	41.0
34	36.40188047185446	39.0	35.0	41.0	30.0	41.0
35	36.252235104501565	39.0	35.0	41.0	30.0	41.0
36	36.05047972769068	39.0	35.0	40.0	29.0	41.0
37	35.986445227084594	39.0	35.0	40.0	29.0	41.0
38	35.832986315411475	38.0	35.0	40.0	28.0	41.0
39	35.768902923211535	38.0	35.0	40.0	28.0	41.0
40	35.58996137564086	38.0	35.0	40.0	27.0	41.0
41	35.44351756472657	38.0	34.0	40.0	26.0	41.0
42	35.38608680112417	38.0	34.0	40.0	26.0	41.0
43	35.22478331751381	38.0	34.0	40.0	25.0	41.0
44	35.05783538368943	38.0	34.0	40.0	24.0	41.0
45	34.879190557515905	38.0	34.0	40.0	24.0	41.0
46	34.717910676743955	38.0	34.0	40.0	23.0	41.0
47	34.5531122871319	38.0	33.0	40.0	23.0	41.0
48	34.45955232159204	37.0	33.0	40.0	23.0	41.0
49	34.280239872274926	37.0	33.0	40.0	23.0	41.0
50	34.12091734117397	37.0	33.0	40.0	22.0	41.0
51	32.10173497721483	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	15.0
10	34.0
11	17.0
12	15.0
13	12.0
14	17.0
15	38.0
16	67.0
17	137.0
18	288.0
19	459.0
20	862.0
21	1382.0
22	2125.0
23	3006.0
24	4208.0
25	5685.0
26	7190.0
27	8269.0
28	9659.0
29	11165.0
30	13447.0
31	16662.0
32	21903.0
33	31037.0
34	44934.0
35	49959.0
36	57906.0
37	89192.0
38	109404.0
39	104031.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.619394115138014	20.442586938526407	24.087033780719516	11.85098516561606
2	33.95689784522944	24.142331859279878	24.347845145149027	17.55292515034165
3	33.302762037869066	23.819647339875814	25.4516150242182	17.42597559803692
4	29.203623372879328	26.2537743467493	25.291958681545445	19.250643598825928
5	22.633604709777813	39.826417982665404	20.423873259501406	17.116104048055377
6	29.422286811576853	35.96937700308017	21.752207286870583	12.856128898472392
7	73.61691581710498	18.271800171626353	5.277088893347203	2.8341951179214666
8	87.24755499882829	3.6707471478498657	6.284930093450381	2.7967677598714658
9	80.6758504186975	6.420140639198582	7.952976402219342	4.951032539884582
10	44.59385415806146	26.16711821144434	15.418048416165247	13.820979214328947
11	30.593324780114273	27.43408485894775	24.523011924491147	17.449578436446828
12	31.110226941291312	22.674235310183445	26.66378936827003	19.551748380255212
13	26.255628855481504	24.641194708243628	28.74134492345094	20.361831512823926
14	21.4010307696717	26.020780613302897	29.36901183345163	23.209176783573774
15	20.199983478013113	27.789138985313976	31.628646427794706	20.38223110887821
16	25.05576170574341	25.96598830985132	29.320457423008385	19.657792561396885
17	25.030135766898372	26.024152447361455	26.950563854950442	21.995147930789734
18	23.95216041837717	26.158014259486233	28.675088384200258	21.214736937936337
19	24.49182245944948	27.731143439506766	26.680479946859897	21.096554154183856
20	26.65249372417386	27.566935120854964	27.099598920338735	18.680972234632446
21	26.999624040502475	26.826143178189625	27.42599245720721	18.74824032410069
22	24.95511245909544	24.58151324540714	28.196119356182002	22.267254939315418
23	23.771261521135497	25.893831060998163	29.238016080276623	21.096891337589714
24	23.761483202365678	24.801694009431017	29.471009813723025	21.965812974480272
25	22.80826571401115	26.817545001340303	27.674328035620054	22.69986124902849
26	22.73341099791115	28.270131113767366	27.373391845893696	21.623066042427787
27	22.96556177284291	27.77413432375339	27.76941375607141	21.49089014733229
28	20.8101168509093	28.020952576839885	28.86273094955905	22.306199622691768
29	23.26632937086634	26.32593159560245	28.247202642169167	22.160536391362033
30	23.875114010139107	26.437707894643676	28.312110447796424	21.3750676474208
31	23.78761491631951	26.778600317963953	27.58750330861217	21.846281457104368
32	25.0593021315049	26.219381639352	27.22149072155563	21.49982550758747
33	24.29693045086479	25.749010788183067	27.590032184156087	22.36402657679605
34	22.802196412705744	25.531527491406038	29.21879662614284	22.447479469745378
35	23.548214698161846	25.311852502490943	28.544092631025258	22.595840168321956
36	23.429188955894727	27.157088691037156	27.24509355996554	22.168628793102577
37	22.546779982769927	27.67651972775812	28.66379274010409	21.112907549367865
38	22.957300779399443	26.539368691509218	28.57207885371129	21.931251675380047
39	23.540796663233017	25.649035908346807	27.71495863602569	23.09520879239449
40	24.727176476736872	25.0822306031031	28.184992303788764	22.005600616371265
41	21.567936555570355	25.285720788537112	29.339845468845095	23.806497187047437
42	21.48987859711472	25.63318828827158	28.76140733609936	24.115525778514336
43	22.437363967569702	25.157759686014813	29.25302074183721	23.151855604578277
44	22.870813235797414	25.33494956579207	27.83364719488695	23.960590003523567
45	22.217520386951676	25.362430013369323	27.457519105654733	24.962530494024268
46	22.26607479739492	26.224945165548625	27.57688203132771	23.932098005728747
47	21.639082254205942	25.039408310559402	30.25344390701156	23.068065528223094
48	22.650295288367676	24.158179479355102	29.95351926750277	23.238005964774448
49	21.928891391539057	24.957472742936428	29.6664075974165	23.447228268108013
50	21.432051643010443	25.002149544212333	29.786107706495336	23.779691106281895
51	20.998939558188585	24.454395101399477	28.914151418952066	25.63251392145987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	385.0
1	910.5
2	1436.0
3	2008.0
4	2580.0
5	2010.5
6	1441.0
7	1224.5
8	1008.0
9	1053.5
10	1099.0
11	1092.0
12	1085.0
13	1051.0
14	1017.0
15	1057.5
16	1098.0
17	1293.5
18	1489.0
19	1561.0
20	1633.0
21	1804.0
22	1975.0
23	1984.5
24	1994.0
25	2743.5
26	4528.5
27	5564.0
28	6975.5
29	8387.0
30	10036.5
31	11686.0
32	13132.5
33	14579.0
34	16436.5
35	18294.0
36	19584.0
37	20874.0
38	22310.5
39	23747.0
40	26362.0
41	28977.0
42	31387.0
43	33797.0
44	36572.0
45	39347.0
46	41888.0
47	44429.0
48	46183.5
49	47938.0
50	45911.5
51	43885.0
52	40752.5
53	37620.0
54	35572.5
55	33525.0
56	33115.0
57	32705.0
58	31075.0
59	29445.0
60	28133.5
61	26822.0
62	23967.0
63	21112.0
64	18223.5
65	15335.0
66	13191.0
67	11047.0
68	9444.0
69	7841.0
70	6601.5
71	5362.0
72	4530.0
73	3698.0
74	2893.5
75	1815.5
76	1542.0
77	1177.0
78	812.0
79	618.0
80	424.0
81	345.5
82	267.0
83	203.5
84	140.0
85	103.0
86	66.0
87	45.0
88	24.0
89	18.0
90	12.0
91	11.5
92	11.0
93	10.5
94	10.0
95	5.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	593149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.73370143058474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57535919044426	25.208108573383353
2	8.112362803728189	5.6354477504255245
3	3.9612835056311795	4.127701156994802
4	2.727423753418041	3.789340893036281
5	1.9186485452953588	3.3320882861257366
6	1.534407079530474	3.197738242403218
7	1.1257756472896077	2.7371648647546354
8	0.8076846972612021	2.2443103299778255
9	0.6483184743723798	2.02666502887042
>10	6.507591470750526	45.03798981778392
>50	0.06134162916359363	1.3345745416156758
>100	0.01883719320714884	1.0783966251777295
>500	9.660099080589148E-4	0.25047388945089455
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.1616794431078869	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6859170292793211E-4	0.0	0.0	0.04872300214617238	0.0
2	1.6859170292793211E-4	0.0	0.0	0.283065469215998	0.0
3	1.6859170292793211E-4	0.0	0.0	0.4229965826461817	0.0
4	1.6859170292793211E-4	0.0	0.0	0.6065929471346997	0.0
5	1.6859170292793211E-4	0.0	0.0	1.1884029139389933	0.0
6	1.6859170292793211E-4	0.0	0.0	1.579367073028868	0.0
7	1.6859170292793211E-4	0.0	0.0	1.9374558500477959	0.0
8	1.6859170292793211E-4	0.0	0.0	2.473071690249836	0.0
9	1.6859170292793211E-4	0.0	0.0	2.691735128947364	0.0
10	1.6859170292793211E-4	0.0	0.0	3.2794458053541353	0.0
11	1.6859170292793211E-4	0.0	0.0	4.126619112566994	0.0
12	1.6859170292793211E-4	0.0	0.0	4.698987944007324	0.0
13	1.6859170292793211E-4	0.0	0.0	4.940074079194266	0.0
14	1.6859170292793211E-4	0.0	0.0	5.04493811841544	0.0
15	1.6859170292793211E-4	0.0	0.0	5.259049581133914	0.0
16	1.6859170292793211E-4	0.0	0.0	5.7110439366837005	0.0
17	1.6859170292793211E-4	0.0	0.0	6.194564940681009	0.0
18	1.6859170292793211E-4	0.0	0.0	6.685503979607148	0.0
19	1.6859170292793211E-4	0.0	0.0	7.00734554049657	0.0
20	1.6859170292793211E-4	0.0	0.0	7.344360354649506	0.0
21	1.6859170292793211E-4	0.0	0.0	7.761287635990282	0.0
22	1.6859170292793211E-4	0.0	0.0	8.199794655305833	0.0
23	1.6859170292793211E-4	0.0	0.0	8.62650025541643	0.0
24	1.6859170292793211E-4	0.0	0.0	8.977002405803601	0.0
25	1.6859170292793211E-4	0.0	0.0	9.311488344412618	0.0
26	1.6859170292793211E-4	0.0	0.0	9.592699304896408	0.0
27	1.6859170292793211E-4	0.0	0.0	9.89346690291984	0.0
28	1.6859170292793211E-4	0.0	0.0	10.196089009675477	0.0
29	1.6859170292793211E-4	0.0	0.0	10.52366268846445	0.0
30	1.6859170292793211E-4	0.0	0.0	10.939578419587658	0.0
31	1.6859170292793211E-4	0.0	0.0	11.278953517581586	0.0
32	1.6859170292793211E-4	0.0	0.0	11.618497207278441	0.0
33	1.6859170292793211E-4	0.0	0.0	11.939664401356152	0.0
34	1.6859170292793211E-4	0.0	0.0	12.26757526355098	0.0
35	1.6859170292793211E-4	0.0	0.0	12.60829909516833	0.0
36	1.6859170292793211E-4	0.0	0.0	12.953912086170591	0.0
37	1.6859170292793211E-4	0.0	0.0	13.299862260578708	0.0
38	3.3718340585586423E-4	0.0	0.0	13.635528341108222	0.0
39	3.3718340585586423E-4	0.0	0.0	14.066448733792015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGTC	35	1.210592E-7	45.0	16
CGCGACA	20	7.030523E-4	45.0	21
ATCCGTA	20	7.030523E-4	45.0	45
CCCCGTC	25	3.8885148E-5	45.0	19
CGGATTC	20	7.030523E-4	45.0	18
CGATAGT	20	7.030523E-4	45.0	10
CCGTTAA	20	7.030523E-4	45.0	30
CTTACGA	20	7.030523E-4	45.0	38
ATTGCGG	70	0.0	41.785713	2
TAACGGG	165	0.0	40.90909	3
ACGGGTC	40	3.4544428E-7	39.375	5
CGCACGG	40	3.4544428E-7	39.375	2
ACGACCA	155	0.0	39.193546	28
TAATGGG	325	0.0	38.76923	3
GATAACG	35	6.2431754E-6	38.571426	1
AATTGCG	35	6.2431754E-6	38.571426	1
TACGAAT	140	0.0	38.571426	12
GCGAGAC	160	0.0	37.96875	21
CGCATAG	30	1.13925555E-4	37.500004	2
CGATTCG	30	1.13925555E-4	37.500004	10
>>END_MODULE