FastQCFastQC Report
Sat 18 Jun 2016
SRR3551124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239997
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC2590.10791801564186218RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC2560.10666800001666688RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT2550.10625132814160176RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.1012512656408205No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC2400.1000012500156252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG253.881553E-545.05
CGTGGGC253.881553E-545.024
CGGGTAC207.0221216E-445.06
TCGTTCC207.0221216E-445.024
TTAATCG351.2065539E-745.020
TAGCGCC207.0221216E-445.019
GCACGTG207.0221216E-445.01
TTCGTTC207.0221216E-445.023
GGTCAAT207.0221216E-445.09
TACGGGC453.8198777E-1045.04
AAGCGCA406.7757355E-945.042
ATCGTTG351.2065539E-745.023
CGTTATT207.0221216E-445.01
GCCGATT253.881553E-545.09
TACGAAT351.2065539E-745.012
TTGACGG207.0221216E-445.02
GCGGGTC253.881553E-545.05
CGAATTG253.881553E-545.033
CGAATAT351.2065539E-745.014
AGCGAAT253.881553E-545.031