Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551124_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 239997 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC | 259 | 0.10791801564186218 | RNA PCR Primer, Index 16 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC | 256 | 0.10666800001666688 | RNA PCR Primer, Index 14 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT | 255 | 0.10625132814160176 | RNA PCR Primer, Index 16 (95% over 24bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 243 | 0.1012512656408205 | No Hit |
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC | 240 | 0.1000012500156252 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATGGGCG | 25 | 3.881553E-5 | 45.0 | 5 |
CGTGGGC | 25 | 3.881553E-5 | 45.0 | 24 |
CGGGTAC | 20 | 7.0221216E-4 | 45.0 | 6 |
TCGTTCC | 20 | 7.0221216E-4 | 45.0 | 24 |
TTAATCG | 35 | 1.2065539E-7 | 45.0 | 20 |
TAGCGCC | 20 | 7.0221216E-4 | 45.0 | 19 |
GCACGTG | 20 | 7.0221216E-4 | 45.0 | 1 |
TTCGTTC | 20 | 7.0221216E-4 | 45.0 | 23 |
GGTCAAT | 20 | 7.0221216E-4 | 45.0 | 9 |
TACGGGC | 45 | 3.8198777E-10 | 45.0 | 4 |
AAGCGCA | 40 | 6.7757355E-9 | 45.0 | 42 |
ATCGTTG | 35 | 1.2065539E-7 | 45.0 | 23 |
CGTTATT | 20 | 7.0221216E-4 | 45.0 | 1 |
GCCGATT | 25 | 3.881553E-5 | 45.0 | 9 |
TACGAAT | 35 | 1.2065539E-7 | 45.0 | 12 |
TTGACGG | 20 | 7.0221216E-4 | 45.0 | 2 |
GCGGGTC | 25 | 3.881553E-5 | 45.0 | 5 |
CGAATTG | 25 | 3.881553E-5 | 45.0 | 33 |
CGAATAT | 35 | 1.2065539E-7 | 45.0 | 14 |
AGCGAAT | 25 | 3.881553E-5 | 45.0 | 31 |