##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	239997
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810576798876653	31.0	31.0	34.0	30.0	34.0
2	31.94015758530315	33.0	31.0	34.0	30.0	34.0
3	31.9033654587349	33.0	31.0	34.0	30.0	34.0
4	35.65997491635312	37.0	35.0	37.0	33.0	37.0
5	31.165147897682054	37.0	35.0	37.0	0.0	37.0
6	33.3753838589649	37.0	35.0	37.0	17.0	37.0
7	29.56999045821406	35.0	32.0	37.0	0.0	37.0
8	32.61549102697117	35.0	33.0	37.0	17.0	37.0
9	36.39661329099947	39.0	35.0	39.0	32.0	39.0
10	36.6668750026042	38.0	35.0	39.0	33.0	39.0
11	36.71584228136185	39.0	35.0	39.0	33.0	39.0
12	36.78333895840365	39.0	35.0	39.0	33.0	39.0
13	36.6105326316579	39.0	35.0	39.0	33.0	39.0
14	37.73700087917766	40.0	37.0	41.0	33.0	41.0
15	37.832593740755094	40.0	37.0	41.0	33.0	41.0
16	37.92339904248803	40.0	37.0	41.0	33.0	41.0
17	37.88333187498177	40.0	36.0	41.0	33.0	41.0
18	37.83498543731797	40.0	36.0	41.0	33.0	41.0
19	37.76882627699513	40.0	36.0	41.0	33.0	41.0
20	37.68387521510686	39.0	36.0	41.0	33.0	41.0
21	37.60322420696925	39.0	36.0	41.0	33.0	41.0
22	37.581403100872095	39.0	36.0	41.0	33.0	41.0
23	37.542685950241044	39.0	36.0	41.0	33.0	41.0
24	37.49972291320308	39.0	36.0	41.0	33.0	41.0
25	37.365979574744685	39.0	35.0	41.0	32.0	41.0
26	37.20506506331329	39.0	35.0	41.0	32.0	41.0
27	37.153564419555245	39.0	35.0	41.0	32.0	41.0
28	37.05432567907099	39.0	35.0	41.0	31.0	41.0
29	36.997087463593296	39.0	35.0	41.0	31.0	41.0
30	36.841552186068995	39.0	35.0	40.0	31.0	41.0
31	36.63520794009925	39.0	35.0	40.0	30.0	41.0
32	36.536798376646374	39.0	35.0	40.0	30.0	41.0
33	36.37561719521494	39.0	35.0	40.0	30.0	41.0
34	36.200023333625005	39.0	35.0	40.0	30.0	41.0
35	36.12793909923874	39.0	35.0	40.0	29.0	41.0
36	35.97967057921557	38.0	35.0	40.0	29.0	41.0
37	35.807580928094936	38.0	35.0	40.0	28.0	41.0
38	35.697700387921515	38.0	35.0	40.0	27.0	41.0
39	35.62338695900365	38.0	35.0	40.0	27.0	41.0
40	35.384925644903895	38.0	34.0	40.0	26.0	41.0
41	35.238748817693555	38.0	34.0	40.0	25.0	41.0
42	35.226652833160415	38.0	34.0	40.0	25.0	41.0
43	35.06195910782218	38.0	34.0	40.0	24.0	41.0
44	34.86229827872848	38.0	34.0	40.0	24.0	41.0
45	34.68843777213882	38.0	33.0	40.0	23.0	41.0
46	34.49248948945195	38.0	33.0	40.0	23.0	41.0
47	34.297612053484	37.0	33.0	40.0	23.0	41.0
48	34.208790109876375	37.0	33.0	40.0	22.0	41.0
49	33.97046213077663	37.0	33.0	40.0	22.0	41.0
50	33.74503847964766	37.0	33.0	40.0	20.0	41.0
51	31.568411271807566	35.0	28.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	5.0
10	8.0
11	6.0
12	3.0
13	2.0
14	10.0
15	8.0
16	43.0
17	48.0
18	103.0
19	173.0
20	320.0
21	550.0
22	860.0
23	1234.0
24	1852.0
25	2341.0
26	2989.0
27	3507.0
28	4128.0
29	4996.0
30	6172.0
31	7699.0
32	9847.0
33	13483.0
34	18803.0
35	21038.0
36	24606.0
37	35999.0
38	41652.0
39	37500.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.85515652278987	19.498160393671586	23.296541206765085	11.35014187677346
2	33.366250411463476	22.805285066063327	25.17656470705884	18.65189981541436
3	32.6483247707263	23.609878456814045	26.045742238444646	17.69605453401501
4	29.906207160922843	25.489901957107797	25.359900332087488	19.24399054988187
5	21.921107347175173	40.2246694750351	20.762759534494183	17.09146364329554
6	29.852456489039447	35.61586186493998	21.766938753401085	12.764742892619491
7	72.00715008937611	18.053975674695934	6.393829922874036	3.5450443130539133
8	83.57146130993304	4.060050750634383	7.989683204373388	4.378804735059188
9	76.8147101838773	6.821335266690834	10.675550111043055	5.6884044383888135
10	42.01427517843973	26.642833035412945	17.39105072146735	13.951841064679977
11	26.937836722959034	27.280341004262553	27.177839722996538	18.603982549781872
12	27.881598519981498	22.560698675400108	30.620382754784437	18.937320049833957
13	24.956561957024462	23.764880394338263	31.429142864285804	19.84941478435147
14	20.24858644066384	26.41908023850298	30.487881098513732	22.844452222319443
15	18.194394096592877	27.837014629349532	33.20833177081381	20.76025950324379
16	23.838631316224788	26.239077988474858	29.680371004637557	20.241919690662797
17	21.767772097151212	26.935336691708645	28.31910398879986	22.97778722234028
18	21.308183018954406	26.439080488506107	31.237473801755854	21.015262690783633
19	21.67527094088676	27.309091363642047	29.212031817064382	21.803605878406813
20	22.03110872219236	29.487035254607346	29.758705317149797	18.723150706050493
21	24.25988658191561	26.297828722859034	30.429130364129552	19.013154331095805
22	22.550698550398547	23.73613003495877	30.157876973462166	23.555294441180514
23	21.285266065825823	24.937811722646533	30.959970332962495	22.81695187856515
24	21.906107159672832	24.392804910061376	31.769563786213993	21.9315241440518
25	20.66400830010375	26.42783034787935	29.583703129622453	23.324458222394444
26	19.443993049913125	27.822014441847188	30.001208348437693	22.732784159801998
27	20.769842956370287	26.73908423855298	30.18662733284166	22.304445472235066
28	18.37106297162048	27.176589707371342	30.41329683287708	24.039050488131103
29	19.173989674870935	25.097813722671535	30.408296770376296	25.319899832081234
30	21.00859594078259	26.405330066625833	27.973683004370887	24.612390988220685
31	20.770259628245352	28.246186410663466	27.59159489493619	23.391959066154993
32	21.356516956461956	26.82825201981691	27.369508785526484	24.445722238194644
33	21.00026250328129	25.5865698321229	27.520760676175122	25.892406988420692
34	18.315228940361756	26.93158664483306	29.463701629603705	25.289482785201482
35	19.38024225302816	26.6015825197815	27.95159939499244	26.066575832197902
36	20.54775684696059	26.978253894840353	27.234507098005388	25.23948216019367
37	20.144418471897566	28.11410142626783	28.426605332566655	23.31487476926795
38	19.66816251869815	27.480343504293803	27.699096238702985	25.15239773830506
39	21.475685112730574	26.14949353533586	27.35700862927453	25.017812722659034
40	21.661520769009613	26.00615841031346	29.385367317091465	22.946953503585462
41	18.37772972162152	26.53658170727134	29.075363442043024	26.010325129064114
42	19.640662174943856	25.898657066546665	28.527856598207478	25.932824160302005
43	20.47692262819952	24.623224456972377	29.12036400455006	25.779488910278047
44	19.817747721846523	25.11948066017492	28.019100238752987	27.043671379225575
45	19.33274165927074	25.306983003954215	28.361187848181434	26.99908748859361
46	21.20943178456397	26.016991879065156	27.12117234798768	25.65240398838319
47	18.248144768476273	25.914907269674202	30.497881223515293	25.339066738334232
48	19.15565611236807	24.966562082026027	29.62870369212948	26.249078113476422
49	20.201502518781485	24.90906136326704	29.734955020271087	25.154481097680385
50	19.280241003012538	23.936965878740153	30.55454859852415	26.228244519723166
51	19.149406034242094	23.136539206740085	28.894944520139838	28.819110238877986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287.0
1	487.0
2	687.0
3	789.0
4	891.0
5	698.0
6	505.0
7	470.0
8	435.0
9	462.5
10	490.0
11	474.0
12	458.0
13	493.5
14	529.0
15	566.0
16	603.0
17	669.0
18	735.0
19	758.5
20	782.0
21	828.0
22	874.0
23	914.5
24	955.0
25	1268.5
26	2217.5
27	2853.0
28	3287.0
29	3721.0
30	4063.0
31	4405.0
32	5171.5
33	5938.0
34	6671.0
35	7404.0
36	8038.0
37	8672.0
38	9499.5
39	10327.0
40	10812.5
41	11298.0
42	12706.5
43	14115.0
44	15259.5
45	16404.0
46	18163.5
47	19923.0
48	21147.5
49	22372.0
50	22105.0
51	21838.0
52	19469.5
53	17101.0
54	15181.5
55	13262.0
56	12160.5
57	11059.0
58	10406.0
59	9753.0
60	9012.0
61	8271.0
62	7320.0
63	6369.0
64	5455.0
65	4541.0
66	4005.0
67	3469.0
68	3026.5
69	2584.0
70	2126.0
71	1668.0
72	1465.5
73	1263.0
74	1015.0
75	554.5
76	342.0
77	282.0
78	222.0
79	176.0
80	130.0
81	90.5
82	51.0
83	44.0
84	37.0
85	25.0
86	13.0
87	10.0
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	239997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.47676845960574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54768291090282	30.919969832956244
2	9.43813226444853	7.829264532473322
3	4.020373105090262	5.002562532031651
4	2.391931125242358	3.9683829381200595
5	1.617391479059301	3.3542085942740956
6	1.1703484926112333	2.9125364067050836
7	0.8076911485488683	2.345029312866411
8	0.6359060908351165	2.1100263753296917
9	0.49727253548717637	1.8562732034150426
>10	4.815004570888962	37.546719333991675
>50	0.04319741217363351	1.0879302657949892
>100	0.015068864711732617	1.0670966720417339
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	259	0.10791801564186218	RNA PCR Primer, Index 16 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	256	0.10666800001666688	RNA PCR Primer, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	255	0.10625132814160176	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.1012512656408205	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	240	0.1000012500156252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.16671875065105E-4	0.0	0.0	0.2266695000354171	0.0
2	4.16671875065105E-4	0.0	0.0	0.8433438751317724	0.0
3	4.16671875065105E-4	0.0	0.0	1.1729313283082705	0.0
4	4.16671875065105E-4	0.0	0.0	1.559186156493623	0.0
5	4.16671875065105E-4	0.0	0.0	2.6829502035442108	0.0
6	4.16671875065105E-4	0.0	0.0	3.2725409067613347	0.0
7	4.16671875065105E-4	0.0	0.0	3.870048375604695	0.0
8	4.16671875065105E-4	0.0	0.0	5.012979328908278	0.0
9	4.16671875065105E-4	0.0	0.0	5.417567719596495	0.0
10	4.16671875065105E-4	0.0	0.0	6.388829860373255	0.0
11	4.16671875065105E-4	0.0	0.0	7.910932219986083	0.0
12	4.16671875065105E-4	0.0	0.0	8.825110313878923	0.0
13	4.16671875065105E-4	0.0	0.0	9.260115751446893	0.0
14	4.16671875065105E-4	0.0	0.0	9.42886786084826	0.0
15	4.16671875065105E-4	0.0	0.0	9.810122626532833	0.0
16	4.16671875065105E-4	0.0	0.0	10.57804889227782	0.0
17	4.16671875065105E-4	0.0	0.0	11.442226361162849	0.0
18	4.16671875065105E-4	0.0	0.0	12.383071455059856	0.0
19	4.16671875065105E-4	0.0	0.0	12.951411892648657	0.0
20	4.16671875065105E-4	0.0	0.0	13.527252423988633	0.0
21	4.16671875065105E-4	0.0	0.0	14.226011158472815	0.0
22	4.16671875065105E-4	0.0	0.0	14.988520689841957	0.0
23	4.16671875065105E-4	0.0	0.0	15.71186306495498	0.0
24	4.16671875065105E-4	0.0	0.0	16.28312020566924	0.0
25	4.16671875065105E-4	0.0	0.0	16.872294237011296	0.0
26	4.16671875065105E-4	0.0	0.0	17.341466768334605	0.0
27	4.16671875065105E-4	0.0	0.0	17.82438947153506	0.0
28	4.16671875065105E-4	0.0	0.0	18.338145893490335	0.0
29	4.16671875065105E-4	0.0	0.0	18.888986112326403	0.0
30	4.16671875065105E-4	0.0	0.0	19.62732867494177	0.0
31	4.16671875065105E-4	0.0	0.0	20.16608540940095	0.0
32	4.16671875065105E-4	0.0	0.0	20.684008550106878	0.0
33	4.16671875065105E-4	0.0	0.0	21.174431347058505	0.0
34	4.16671875065105E-4	0.0	0.0	21.71110472214236	0.0
35	4.16671875065105E-4	0.0	0.0	22.267361675354277	0.0
36	4.16671875065105E-4	0.0	0.0	22.748201019179408	0.0
37	4.16671875065105E-4	0.0	0.0	23.22820701925441	0.0
38	4.16671875065105E-4	0.0	0.0	23.740713425584488	0.0
39	4.16671875065105E-4	0.0	0.0	24.326554081926023	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	25	3.881553E-5	45.0	5
CGTGGGC	25	3.881553E-5	45.0	24
CGGGTAC	20	7.0221216E-4	45.0	6
TCGTTCC	20	7.0221216E-4	45.0	24
TTAATCG	35	1.2065539E-7	45.0	20
TAGCGCC	20	7.0221216E-4	45.0	19
GCACGTG	20	7.0221216E-4	45.0	1
TTCGTTC	20	7.0221216E-4	45.0	23
GGTCAAT	20	7.0221216E-4	45.0	9
TACGGGC	45	3.8198777E-10	45.0	4
AAGCGCA	40	6.7757355E-9	45.0	42
ATCGTTG	35	1.2065539E-7	45.0	23
CGTTATT	20	7.0221216E-4	45.0	1
GCCGATT	25	3.881553E-5	45.0	9
TACGAAT	35	1.2065539E-7	45.0	12
TTGACGG	20	7.0221216E-4	45.0	2
GCGGGTC	25	3.881553E-5	45.0	5
CGAATTG	25	3.881553E-5	45.0	33
CGAATAT	35	1.2065539E-7	45.0	14
AGCGAAT	25	3.881553E-5	45.0	31
>>END_MODULE