FastQCFastQC Report
Sat 18 Jun 2016
SRR3551123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274188
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.10029614716909566No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT302.159435E-645.0000048
TCGAGCG302.159435E-645.0000041
CAACGTC207.0238794E-445.020
ACACCGC207.0238794E-445.033
ATAGCGT207.0238794E-445.044
ACGGGTC207.0238794E-445.05
ACGGGTA207.0238794E-445.05
CGTTAGC207.0238794E-445.035
TCTACGG351.2074088E-745.02
TACGATA406.7830115E-945.016
GTAGACG207.0238794E-445.01
TACGAAT406.7830115E-945.012
CGAATAT406.7830115E-945.014
GCTAGCT207.0238794E-445.039
GGTGATC207.0238794E-445.08
ATTGGCG253.8830112E-545.012
TTACGAT406.7830115E-945.015
TAGGCGA207.0238794E-445.06
GGTTAAC351.2074088E-745.09
CCCTACA2300.042.06521627