##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274188
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12114680438239	33.0	31.0	34.0	30.0	34.0
2	32.26497877368813	33.0	31.0	34.0	30.0	34.0
3	32.22150130567348	34.0	31.0	34.0	30.0	34.0
4	35.91382919748494	37.0	35.0	37.0	35.0	37.0
5	31.24844996863466	37.0	35.0	37.0	0.0	37.0
6	33.51298014500999	37.0	35.0	37.0	17.0	37.0
7	29.65378499423753	35.0	32.0	37.0	0.0	37.0
8	32.74884750609071	35.0	35.0	37.0	17.0	37.0
9	36.693947218696664	39.0	35.0	39.0	32.0	39.0
10	36.93108013479802	38.0	37.0	39.0	34.0	39.0
11	37.02963659970531	39.0	37.0	39.0	34.0	39.0
12	37.11983383663764	39.0	37.0	39.0	34.0	39.0
13	36.99257443797686	39.0	37.0	39.0	33.0	39.0
14	38.326170364859145	40.0	38.0	41.0	34.0	41.0
15	38.3614199016733	40.0	38.0	41.0	34.0	41.0
16	38.40711117919092	40.0	38.0	41.0	34.0	41.0
17	38.349588603439976	40.0	37.0	41.0	34.0	41.0
18	38.310480400309274	40.0	37.0	41.0	34.0	41.0
19	38.22788743489868	40.0	37.0	41.0	34.0	41.0
20	38.18943206850774	40.0	37.0	41.0	34.0	41.0
21	38.09053642026639	40.0	37.0	41.0	34.0	41.0
22	38.081954717201334	40.0	37.0	41.0	34.0	41.0
23	38.0088297080835	40.0	37.0	41.0	34.0	41.0
24	37.96826994616832	40.0	37.0	41.0	33.0	41.0
25	37.84749879644623	40.0	36.0	41.0	33.0	41.0
26	37.70531168395407	40.0	36.0	41.0	33.0	41.0
27	37.677476767765185	40.0	36.0	41.0	33.0	41.0
28	37.57190321968868	40.0	36.0	41.0	33.0	41.0
29	37.565761448349306	40.0	36.0	41.0	33.0	41.0
30	37.43583964287277	40.0	36.0	41.0	33.0	41.0
31	37.329208426335214	40.0	36.0	41.0	32.0	41.0
32	37.22466336965877	39.0	35.0	41.0	32.0	41.0
33	37.09982931419318	39.0	35.0	41.0	31.0	41.0
34	36.99973740645105	39.0	35.0	41.0	31.0	41.0
35	36.85502647818285	39.0	35.0	41.0	31.0	41.0
36	36.72098341284082	39.0	35.0	41.0	31.0	41.0
37	36.63023910601485	39.0	35.0	41.0	30.0	41.0
38	36.453221876960335	39.0	35.0	41.0	30.0	41.0
39	36.47908369439946	39.0	35.0	41.0	30.0	41.0
40	36.336090565597324	39.0	35.0	40.0	30.0	41.0
41	36.19364450668884	39.0	35.0	40.0	30.0	41.0
42	36.15854085517966	39.0	35.0	40.0	30.0	41.0
43	36.001838154842666	39.0	35.0	40.0	29.0	41.0
44	35.78308678716793	38.0	35.0	40.0	28.0	41.0
45	35.71072402876858	38.0	35.0	40.0	28.0	41.0
46	35.56917881161831	38.0	35.0	40.0	27.0	41.0
47	35.397110741535	38.0	34.0	40.0	26.0	41.0
48	35.327935577049324	38.0	34.0	40.0	26.0	41.0
49	35.199282973726056	38.0	34.0	40.0	26.0	41.0
50	35.061253592425636	38.0	34.0	40.0	26.0	41.0
51	33.24464965644011	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	7.0
10	9.0
11	8.0
12	4.0
13	3.0
14	12.0
15	20.0
16	21.0
17	50.0
18	86.0
19	166.0
20	285.0
21	472.0
22	732.0
23	1022.0
24	1421.0
25	1831.0
26	2487.0
27	2907.0
28	3440.0
29	4097.0
30	5106.0
31	6496.0
32	8781.0
33	12562.0
34	19113.0
35	22643.0
36	27204.0
37	43614.0
38	55485.0
39	54093.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67595956059346	19.792624038980552	23.642537237224097	10.88887916320189
2	33.21443680978015	24.36539892336645	23.767269172976206	18.652895093877195
3	32.63198972967453	24.955140268720733	25.338089194275458	17.07478080732928
4	27.911141260740806	27.21818606211796	25.144791165185932	19.7258815119553
5	22.762484134973086	40.18666024771325	20.313069864472553	16.737785752841116
6	28.745969918450115	35.74992341021489	22.23802646359432	13.266080207740675
7	75.91761856828163	17.75679460807913	3.968809721796723	2.3567771018425314
8	90.19650750579893	2.8535165652763794	4.809473791704962	2.140502137219718
9	84.11309028841525	6.099464600930748	6.25629130377697	3.5311538068770334
10	38.7912673056443	34.354530468145946	13.66288823726786	13.191313988941895
11	27.57341677972778	28.384174362116504	27.28894043502998	16.75346842312574
12	27.349482836593868	26.198812493617517	27.903482282229714	18.5482223875589
13	23.820152596029	29.063635170029322	28.508541584606185	18.607670649335493
14	22.825214816111572	27.310823230775966	27.525639342349045	22.338322610763417
15	21.122003880549112	27.93776532889842	30.670926517571885	20.26930427298058
16	24.086028564342712	26.247319357521114	28.845536639094345	20.821115439041826
17	23.389790946357973	26.2272601280873	28.626343968372066	21.756604957182663
18	24.240666987614336	25.6119888543627	27.912600113790536	22.234744044232425
19	22.48530205552395	29.12563642464295	26.819189753016175	21.569871766816927
20	26.58686740484631	27.032911724801963	27.17697346346303	19.203247406888703
21	25.184544910791136	27.31884692254949	27.295869987016207	20.200738179643167
22	24.645863422177484	25.39717274278962	26.334850540505055	23.622113294527843
23	23.850788510073382	27.237880578289346	27.058806366434705	21.852524545202563
24	22.340146177075585	26.865873050607615	30.778881643252078	20.01509912906473
25	23.1673158562738	26.970181043663473	28.078179934935154	21.784323165127574
26	22.754460443199555	29.827344741564183	26.617138605628256	20.801056209608006
27	22.138824456212525	27.70981953987775	27.43008446759158	22.721271536318145
28	20.783914686273654	29.151531066275695	28.71679285745547	21.347761389995185
29	21.911243380454287	26.203918479291584	27.737173034560232	24.147665105693903
30	23.515981735159816	28.19306461260157	27.51250966490145	20.778443987337155
31	23.701620785738253	28.42502224750901	26.587596831371172	21.28576013538156
32	22.560797700847594	28.32436138707748	26.29983806731148	22.815002844763445
33	22.635928632908808	27.92791807081273	26.33229754766802	23.103855748610442
34	21.786876157964606	27.189738427648187	27.845857586765284	23.177527827621923
35	22.271580083738165	28.374691817293247	26.831225290676468	22.52250280829212
36	23.062643149955505	29.95535909667819	25.708637868907463	21.273359884458838
37	23.783316556523264	28.460399433965016	27.233868732402584	20.522415277109136
38	22.56262126715976	27.003734663807315	29.007104614352198	21.42653945468073
39	24.06560462164646	27.8713875151356	26.7863655593972	21.276642303820736
40	24.475907041883673	27.33635315914628	27.85825783768801	20.32948196128204
41	20.91885859337389	28.057391278976468	28.875807839876288	22.147942287773354
42	22.289086320334953	27.427531474754545	27.632135614979504	22.651246589930995
43	22.434242198783316	27.832363196055258	27.291858141129445	22.441536464031977
44	22.20337870366318	28.27950165579821	26.927509591958803	22.589610048579807
45	22.757013436036587	27.08178330196799	26.04563292339563	24.11557033859979
46	23.083796519176623	28.154405006783666	26.23090726071163	22.53089121332808
47	21.746757699096968	26.845813821173792	29.90174624710053	21.505682232628708
48	23.19831648358061	25.70097889039637	29.055611478255795	22.045093147767226
49	21.697521408668504	28.078179934935154	29.02825798357331	21.196040672823028
50	21.70262739434257	27.72841991626184	28.701110187170848	21.86784250222475
51	21.864924796125287	25.91214786934512	28.180299648416412	24.04262768611318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	411.5
2	671.0
3	797.0
4	923.0
5	743.0
6	563.0
7	523.0
8	483.0
9	514.0
10	545.0
11	529.5
12	514.0
13	529.0
14	544.0
15	567.0
16	590.0
17	587.0
18	584.0
19	648.5
20	713.0
21	806.5
22	900.0
23	1073.0
24	1246.0
25	1728.5
26	2728.5
27	3246.0
28	3654.0
29	4062.0
30	4906.0
31	5750.0
32	6573.5
33	7397.0
34	8370.0
35	9343.0
36	10066.0
37	10789.0
38	11504.5
39	12220.0
40	12845.5
41	13471.0
42	15187.5
43	16904.0
44	18510.5
45	20117.0
46	20338.0
47	20559.0
48	20929.5
49	21300.0
50	20769.0
51	20238.0
52	19480.5
53	18723.0
54	17519.5
55	16316.0
56	15175.0
57	14034.0
58	12789.0
59	11544.0
60	10741.0
61	9938.0
62	9465.5
63	8993.0
64	7283.5
65	5574.0
66	4643.0
67	3712.0
68	3228.5
69	2745.0
70	2312.0
71	1879.0
72	1782.0
73	1685.0
74	1449.0
75	1072.5
76	932.0
77	635.0
78	338.0
79	263.0
80	188.0
81	174.0
82	160.0
83	122.5
84	85.0
85	68.5
86	52.0
87	40.0
88	28.0
89	18.0
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	274188.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.36214568106555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2965291063132	23.118808992370198
2	8.627493850778901	5.756634134243658
3	4.671221645258267	4.67525931112959
4	3.1757310740639517	4.237968109472333
5	2.1710849959005194	3.621602695960436
6	1.6212079803224926	3.245218609129502
7	1.2014211533205794	2.8057391278976467
8	0.9499863350642253	2.5354866004347385
9	0.8264553156600164	2.4815090375946434
>10	7.396556436184749	45.84518651436241
>50	0.05356654823722328	1.141917224677958
>100	0.008745558895873189	0.5346696427268881
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.10029614716909566	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04996571695333129	0.0
2	0.0	0.0	0.0	0.22940464207040426	0.0
3	3.647132624330751E-4	0.0	0.0	0.32021824441623997	0.0
4	3.647132624330751E-4	0.0	0.0	0.4511503056297139	0.0
5	3.647132624330751E-4	0.0	0.0	0.8027338906151983	0.0
6	3.647132624330751E-4	0.0	0.0	1.0080674573650197	0.0
7	3.647132624330751E-4	0.0	0.0	1.2206952893635024	0.0
8	3.647132624330751E-4	0.0	0.0	1.5770201467606169	0.0
9	3.647132624330751E-4	0.0	0.0	1.69008125811487	0.0
10	3.647132624330751E-4	0.0	0.0	2.050417961398748	0.0
11	3.647132624330751E-4	0.0	0.0	2.602229127459991	0.0
12	3.647132624330751E-4	0.0	0.0	2.9946605978379797	0.0
13	3.647132624330751E-4	0.0	0.0	3.165711117919092	0.0
14	3.647132624330751E-4	0.0	0.0	3.2255240929581164	0.0
15	3.647132624330751E-4	0.0	0.0	3.3779742366551417	0.0
16	3.647132624330751E-4	0.0	0.0	3.728098968590894	0.0
17	3.647132624330751E-4	0.0	0.0	4.184355259894671	0.0
18	3.647132624330751E-4	0.0	0.0	4.616905189140298	0.0
19	3.647132624330751E-4	0.0	0.0	4.8568865158212615	0.0
20	3.647132624330751E-4	0.0	0.0	5.112185799524414	0.0
21	3.647132624330751E-4	0.0	0.0	5.445533721388244	0.0
22	3.647132624330751E-4	0.0	0.0	5.811341123608619	0.0
23	3.647132624330751E-4	0.0	0.0	6.170948400367631	0.0
24	3.647132624330751E-4	0.0	0.0	6.470377988825185	0.0
25	3.647132624330751E-4	0.0	0.0	6.726041985790771	0.0
26	3.647132624330751E-4	0.0	0.0	6.971858724670664	0.0
27	3.647132624330751E-4	0.0	0.0	7.194698528017272	0.0
28	3.647132624330751E-4	0.0	0.0	7.444162399521496	0.0
29	3.647132624330751E-4	0.0	0.0	7.702379389324113	0.0
30	3.647132624330751E-4	0.0	0.0	8.020409354165755	0.0
31	3.647132624330751E-4	0.0	0.0	8.282638189855136	0.0
32	3.647132624330751E-4	0.0	0.0	8.56419682845347	0.0
33	3.647132624330751E-4	0.0	0.0	8.827155090667716	0.0
34	3.647132624330751E-4	0.0	0.0	9.0882897865698	0.0
35	3.647132624330751E-4	0.0	0.0	9.405955038149008	0.0
36	3.647132624330751E-4	0.0	0.0	9.705749339868994	0.0
37	3.647132624330751E-4	0.0	0.0	9.987672691729761	0.0
38	3.647132624330751E-4	0.0	0.0	10.267043050753498	0.0
39	3.647132624330751E-4	0.0	0.0	10.581061169708375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTT	30	2.159435E-6	45.000004	8
TCGAGCG	30	2.159435E-6	45.000004	1
CAACGTC	20	7.0238794E-4	45.0	20
ACACCGC	20	7.0238794E-4	45.0	33
ATAGCGT	20	7.0238794E-4	45.0	44
ACGGGTC	20	7.0238794E-4	45.0	5
ACGGGTA	20	7.0238794E-4	45.0	5
CGTTAGC	20	7.0238794E-4	45.0	35
TCTACGG	35	1.2074088E-7	45.0	2
TACGATA	40	6.7830115E-9	45.0	16
GTAGACG	20	7.0238794E-4	45.0	1
TACGAAT	40	6.7830115E-9	45.0	12
CGAATAT	40	6.7830115E-9	45.0	14
GCTAGCT	20	7.0238794E-4	45.0	39
GGTGATC	20	7.0238794E-4	45.0	8
ATTGGCG	25	3.8830112E-5	45.0	12
TTACGAT	40	6.7830115E-9	45.0	15
TAGGCGA	20	7.0238794E-4	45.0	6
GGTTAAC	35	1.2074088E-7	45.0	9
CCCTACA	230	0.0	42.065216	27
>>END_MODULE