##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551122_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337682
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7615211944966	31.0	31.0	34.0	30.0	34.0
2	31.89428811722271	31.0	31.0	34.0	30.0	34.0
3	31.848718616923616	33.0	31.0	34.0	30.0	34.0
4	35.6003281193549	37.0	35.0	37.0	33.0	37.0
5	31.012793101201723	37.0	35.0	37.0	0.0	37.0
6	33.28459319714998	37.0	35.0	37.0	17.0	37.0
7	29.778806687949018	35.0	32.0	37.0	0.0	37.0
8	32.761521194496595	35.0	35.0	37.0	17.0	37.0
9	36.52741928796915	39.0	35.0	39.0	32.0	39.0
10	36.651370224056954	38.0	35.0	39.0	32.0	39.0
11	36.753534390343575	39.0	35.0	39.0	33.0	39.0
12	36.83386440497273	39.0	35.0	39.0	33.0	39.0
13	36.694431447338026	39.0	35.0	39.0	33.0	39.0
14	37.839452502650424	40.0	37.0	41.0	33.0	41.0
15	37.91970256039706	40.0	37.0	41.0	33.0	41.0
16	37.96984440982936	40.0	37.0	41.0	33.0	41.0
17	37.91414407637955	40.0	37.0	41.0	33.0	41.0
18	37.86422432939867	40.0	36.0	41.0	33.0	41.0
19	37.77674853856587	40.0	36.0	41.0	33.0	41.0
20	37.72291978844001	39.0	36.0	41.0	33.0	41.0
21	37.6380559224359	39.0	36.0	41.0	33.0	41.0
22	37.603206567125284	39.0	36.0	41.0	33.0	41.0
23	37.530534645021056	39.0	36.0	41.0	33.0	41.0
24	37.49843047600997	39.0	36.0	41.0	32.0	41.0
25	37.40207058712043	39.0	36.0	41.0	32.0	41.0
26	37.21907297398144	39.0	35.0	41.0	32.0	41.0
27	37.18488104192702	39.0	35.0	41.0	32.0	41.0
28	37.077493025983024	39.0	35.0	41.0	31.0	41.0
29	37.03492931219313	39.0	35.0	41.0	31.0	41.0
30	36.88472586634763	39.0	35.0	40.0	31.0	41.0
31	36.79054850421402	39.0	35.0	40.0	31.0	41.0
32	36.65869072085572	39.0	35.0	40.0	30.0	41.0
33	36.482329528965124	39.0	35.0	40.0	30.0	41.0
34	36.382318275774246	39.0	35.0	40.0	30.0	41.0
35	36.28963344211418	39.0	35.0	40.0	30.0	41.0
36	36.13033267985857	39.0	35.0	40.0	29.0	41.0
37	36.06818545258557	38.0	35.0	40.0	29.0	41.0
38	35.856666923318386	38.0	35.0	40.0	28.0	41.0
39	35.83700345295278	38.0	35.0	40.0	28.0	41.0
40	35.70970025053157	38.0	35.0	40.0	27.0	41.0
41	35.61918313679734	38.0	35.0	40.0	27.0	41.0
42	35.57066707730942	38.0	35.0	40.0	27.0	41.0
43	35.35213603330944	38.0	34.0	40.0	26.0	41.0
44	35.1513465331288	38.0	34.0	40.0	25.0	41.0
45	34.99267950320124	38.0	34.0	40.0	24.0	41.0
46	34.82105057420887	38.0	34.0	40.0	24.0	41.0
47	34.643004365053514	38.0	33.0	40.0	23.0	41.0
48	34.541731569938584	38.0	33.0	40.0	23.0	41.0
49	34.35048062970487	37.0	33.0	40.0	23.0	41.0
50	34.158104370383974	37.0	33.0	40.0	22.0	41.0
51	31.98165137614679	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	9.0
11	2.0
12	4.0
13	6.0
14	11.0
15	13.0
16	36.0
17	70.0
18	140.0
19	278.0
20	423.0
21	769.0
22	1166.0
23	1752.0
24	2510.0
25	3160.0
26	3845.0
27	4555.0
28	5479.0
29	6574.0
30	8000.0
31	10277.0
32	13219.0
33	18165.0
34	25184.0
35	30034.0
36	34974.0
37	51080.0
38	61108.0
39	54829.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35035921369809	19.585586439312728	24.443707393346404	10.620346953642775
2	32.607601234297356	23.54789417262395	24.52721791507987	19.317286677998826
3	32.95467333171445	23.77325412666355	25.937420413288244	17.334652128333758
4	27.98698183498084	27.4483093561398	24.891169798804793	19.673539010074567
5	21.990215646673498	40.07735088041412	20.52789310653218	17.404540366380203
6	27.116932498622965	36.26015008202984	23.306838978684087	13.316078440663109
7	73.59705284853797	17.286677998827297	6.001800510539502	3.114468642095226
8	85.29859453568743	3.6107935868657495	7.0462743054115995	4.044337572035229
9	79.21713327923905	6.402769469500891	9.157432140297676	5.222665110962385
10	35.98326235926108	32.806605030768594	17.03022370158907	14.17990890838126
11	25.58264876422196	27.804857824817432	28.811129998045498	17.80136341291511
12	25.729532518760255	24.373226882096173	30.82722798372433	19.07001261541924
13	23.655984032314425	27.79952736598338	30.965523776807768	17.578964824894427
14	20.532927428764342	27.785608945694467	29.85826902233462	21.823194603206566
15	19.687457430363477	28.027552549440003	32.52616366877714	19.758826351419383
16	22.35179843758329	27.063924046884345	30.60275643949041	19.981521076041954
17	21.46220408550056	26.74794629266588	28.365148275596567	23.424701346236994
18	22.13858008422125	26.044621863173045	30.388649676322693	21.428148376283012
19	20.84772063657524	28.819125686296577	28.53068863605404	21.802465041074147
20	22.3227770505979	28.7288040227196	29.682363880810943	19.26605504587156
21	23.2671566740306	28.1922045000918	29.809110346420596	18.731528479457005
22	22.537772223571288	24.747543546887307	29.037674498492667	23.677009731048738
23	20.35406092122174	28.07996872797484	29.60418381791153	21.961786532891892
24	21.00941122120812	25.663197919936508	31.781972388223238	21.545418470632132
25	20.054962953311104	27.0405292553349	29.119408200614778	23.785099590739218
26	18.74426235333835	28.534834548480525	30.590022565609065	22.130880532572064
27	20.342511593747965	27.880076521697926	28.80639181241523	22.971020072138877
28	17.23159659087544	28.901747798224363	29.695690027896067	24.17096558300413
29	18.725901884021063	26.466024247664965	30.27374867478871	24.53432519352527
30	20.736373274263954	26.052617551424124	30.614898040167972	22.59611113414396
31	20.1731214574659	28.10247510971861	26.663843497728635	25.060559935086857
32	19.929104897507123	27.086134292026227	29.089794540425608	23.894966270041046
33	19.37947536439609	26.739062194609133	29.34239906183925	24.539063379155536
34	17.351531914641587	26.66354736112674	30.865725741970255	25.119194982261416
35	17.846672313004543	26.517255879792227	30.951901493120747	24.68417031408248
36	19.31935963421207	26.82731090197286	29.84257378243436	24.010755681380708
37	19.22133841898591	26.98130193495656	29.020794712184838	24.776564933872695
38	18.909506577193927	28.067827127297278	29.137472533330172	23.885193762178616
39	20.575274962834854	26.44944059795903	30.20563725635361	22.769647182852506
40	20.874965203949277	25.3451472095048	29.837835596804098	23.942051989741827
41	18.080916365100894	24.557423848472823	29.501424417055098	27.860235369371182
42	18.831030377692624	24.31251887870837	29.98916140037076	26.867289343228247
43	19.459728383508747	25.146439549635456	29.78068123263899	25.613150834216807
44	18.29857676749131	26.56197250667788	28.62071416302912	26.51873656280169
45	19.749053843556954	24.474505599943143	29.1724166523534	26.604023904146505
46	20.511309456826247	24.746655137081632	28.975485812095403	25.766549593996718
47	17.554385486937417	24.58259545963362	32.399121066565584	25.463897986863383
48	19.146415858707304	23.740086827251673	31.382187975669417	25.731309338371606
49	20.412695968396303	23.55144781184665	31.294531541509468	24.74132467824758
50	18.94119319359634	24.091897110299037	30.936206253220483	26.030703442884136
51	18.642687498889487	24.097523705734982	28.842816614447912	28.41697218092762
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	631.5
2	1031.0
3	1064.0
4	1097.0
5	859.5
6	622.0
7	572.5
8	523.0
9	652.5
10	782.0
11	748.0
12	714.0
13	752.0
14	790.0
15	837.0
16	884.0
17	809.0
18	734.0
19	761.5
20	789.0
21	837.5
22	886.0
23	1371.0
24	1856.0
25	1998.5
26	2565.0
27	2989.0
28	4291.5
29	5594.0
30	6370.0
31	7146.0
32	7746.5
33	8347.0
34	9756.5
35	11166.0
36	11948.0
37	12730.0
38	13656.0
39	14582.0
40	16174.5
41	17767.0
42	19784.0
43	21801.0
44	24172.5
45	26544.0
46	29279.0
47	32014.0
48	33602.5
49	35191.0
50	34017.0
51	32843.0
52	29068.5
53	25294.0
54	21615.5
55	17937.0
56	15617.0
57	13297.0
58	11916.0
59	10535.0
60	9627.5
61	8720.0
62	7609.5
63	6499.0
64	5557.5
65	4616.0
66	3565.0
67	2514.0
68	2159.0
69	1804.0
70	1477.0
71	1150.0
72	1140.5
73	1131.0
74	1037.5
75	817.0
76	690.0
77	509.0
78	328.0
79	242.0
80	156.0
81	151.5
82	147.0
83	111.5
84	76.0
85	49.5
86	23.0
87	19.0
88	15.0
89	10.0
90	5.0
91	3.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	337682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.36095520359862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89617145568266	29.086238943845682
2	10.115896122972522	7.963426682811568
3	4.109282785488689	4.852358869156178
4	2.4658300828331647	3.8822970972032835
5	1.600416616711981	3.149696337874758
6	1.1571458640667203	2.7327819911735762
7	0.8445246295570068	2.326890727863035
8	0.6637650679102438	2.0901141682982964
9	0.5041513691240657	1.785949151033274
>10	4.4929996276962605	37.15737698591728
>50	0.12659483937341254	3.169002519492374
>100	0.022472456693505186	1.6523157907736568
>500	7.490818897835062E-4	0.15155073455704132
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	514	0.1522142133723444	No Hit
GGAAAGAGACATAAATTGGAAATTCAAAATAGTCTGATTCAAGCTTTGGAG	392	0.11608554794155447	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363	0.10749758648669458	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	361	0.10690531328291114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21203380695447197	0.0
2	0.0	0.0	0.0	0.8499120474292382	0.0
3	0.0	0.0	0.0	1.135387731652857	0.0
4	0.0	0.0	0.0	1.5156271284818261	0.0
5	0.0	0.0	0.0	2.515976569672058	0.0
6	0.0	0.0	0.0	3.122760466948194	0.0
7	0.0	0.0	0.0	3.720364129565686	0.0
8	0.0	0.0	0.0	4.912610088781753	0.0
9	0.0	0.0	0.0	5.299956764056124	0.0
10	0.0	0.0	0.0	6.281649599327178	0.0
11	0.0	0.0	0.0	7.858280867798698	0.0
12	0.0	0.0	0.0	8.901866252865121	0.0
13	0.0	0.0	0.0	9.328599096191091	0.0
14	0.0	0.0	0.0	9.48851286121262	0.0
15	0.0	0.0	0.0	9.876155673088883	0.0
16	0.0	0.0	0.0	10.739393867603248	0.0
17	0.0	0.0	0.0	11.818219508294787	0.0
18	0.0	0.0	0.0	12.884607411706872	0.0
19	0.0	0.0	0.0	13.48487630374139	0.0
20	0.0	0.0	0.0	14.108539987325354	0.0
21	0.0	0.0	0.0	15.000207295621324	0.0
22	0.0	0.0	0.0	15.862260943728122	0.0
23	0.0	0.0	0.0	16.7500784761995	0.0
24	2.961366018917206E-4	0.0	0.0	17.43474629977316	0.0
25	2.961366018917206E-4	0.0	0.0	18.074401359859277	0.0
26	2.961366018917206E-4	0.0	0.0	18.65512523616894	0.0
27	2.961366018917206E-4	0.0	0.0	19.242660254322114	0.0
28	2.961366018917206E-4	0.0	0.0	19.827826179660153	0.0
29	2.961366018917206E-4	0.0	0.0	20.440236672372233	0.0
30	2.961366018917206E-4	0.0	0.0	21.175543854869375	0.0
31	2.961366018917206E-4	0.0	0.0	21.82171392019711	0.0
32	8.884098056751618E-4	0.0	0.0	22.452484882226475	0.0
33	8.884098056751618E-4	0.0	0.0	23.061934008919636	0.0
34	8.884098056751618E-4	0.0	0.0	23.63791969959903	0.0
35	8.884098056751618E-4	0.0	0.0	24.25743747075651	0.0
36	8.884098056751618E-4	0.0	0.0	24.855337269975895	0.0
37	8.884098056751618E-4	0.0	0.0	25.43931864890637	0.0
38	8.884098056751618E-4	0.0	0.0	26.046102546182503	0.0
39	8.884098056751618E-4	0.0	0.0	26.684276923259162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	75	0.0	45.000004	5
GACGTGC	35	1.2085184E-7	45.000004	43
GCTAACG	35	1.2085184E-7	45.000004	1
CGGGTCC	25	3.8849343E-5	45.0	6
ACAGTCG	20	7.026202E-4	45.0	26
CGTGCAG	20	7.026202E-4	45.0	16
CGGCATG	25	3.8849343E-5	45.0	33
TTCACGG	45	3.8380676E-10	45.0	2
TGACGTA	20	7.026202E-4	45.0	20
CGACAAG	20	7.026202E-4	45.0	41
CTGACGT	20	7.026202E-4	45.0	19
ACGGGTC	45	3.8380676E-10	45.0	5
ACGGGCC	25	3.8849343E-5	45.0	5
ATCGTCT	20	7.026202E-4	45.0	37
CCGTAAC	20	7.026202E-4	45.0	19
GCTTACA	25	3.8849343E-5	45.0	32
ACGTGCA	20	7.026202E-4	45.0	15
TCGTCTC	20	7.026202E-4	45.0	38
TTGCGTA	25	3.8849343E-5	45.0	20
GCGATTC	25	3.8849343E-5	45.0	9
>>END_MODULE