FastQCFastQC Report
Sat 18 Jun 2016
SRR3551120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243818
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6540.2682328622168995No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC4120.16897850035682355No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG3980.1632365124806208No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT3530.14478012287854053No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC3310.1357569990730791No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.10745720168322272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGG351.206663E-745.0000042
GAGTCTC351.206663E-745.00000427
GTAATCC351.206663E-745.0000049
CACCTAT351.206663E-745.00000415
CGTGTCC351.206663E-745.00000433
CGCACGG351.206663E-745.0000042
TGTCCGT351.206663E-745.00000435
AACCGTC207.022344E-445.025
ACGCCTA207.022344E-445.012
TTAATAG207.022344E-445.01
GCGAATC253.881737E-545.01
TAGACGG253.881737E-545.02
TCCGAGG207.022344E-445.02
CGCATGG502.1827873E-1145.02
CGGCAGT406.7775545E-945.012
TAGCGAG207.022344E-445.01
GCTATCA253.881737E-545.09
TTCGTGG207.022344E-445.016
CGTCTGT207.022344E-445.019
TTGTACC207.022344E-445.022