FastQCFastQC Report
Sat 18 Jun 2016
SRR3551117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708773
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33040.46615771198959327No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.13262356212778986No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8990.12683891739668413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG207.0314534E-445.0000041
AATCCGG207.0314534E-445.0000042
CTAACGG351.2110468E-745.0000042
CTACGAA1250.041.39999811
CGAATAT1250.041.39999814
CGTTTTT11500.041.2826081
GCGATAT556.002665E-1140.9090929
ACGCTAG451.9264917E-840.01
TACGAAT1300.039.80769312
ATGGGCG1250.039.65
CGTTATT2450.039.4897961
TTACGGG1200.039.3750043
CACGACC1950.038.07692327
ATTACGG301.1394803E-437.5000042
TACTATC301.1394803E-437.50000440
TATAGCG301.1394803E-437.5000041
ACCCGTG301.1394803E-437.50000437
TATGGGA4750.037.421054
AAACACG2300.037.17391240
ACGACCA2000.037.12528