##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893906793853603	31.0	31.0	34.0	30.0	34.0
2	32.03096054731205	33.0	31.0	34.0	30.0	34.0
3	31.87808367418059	33.0	31.0	34.0	30.0	34.0
4	35.65808234794497	37.0	35.0	37.0	33.0	37.0
5	31.11079287726818	37.0	35.0	37.0	0.0	37.0
6	33.366685807726874	37.0	35.0	37.0	17.0	37.0
7	29.6365620586563	35.0	32.0	37.0	0.0	37.0
8	32.708084252645065	35.0	35.0	37.0	17.0	37.0
9	36.58979814411666	39.0	35.0	39.0	32.0	39.0
10	36.87434481843975	38.0	37.0	39.0	33.0	39.0
11	36.93437108919217	39.0	37.0	39.0	33.0	39.0
12	36.91216792964743	39.0	35.0	39.0	33.0	39.0
13	36.7277083071731	39.0	35.0	39.0	33.0	39.0
14	37.83133951208638	40.0	37.0	41.0	33.0	41.0
15	37.97923735808221	40.0	37.0	41.0	33.0	41.0
16	38.0634039389198	40.0	37.0	41.0	33.0	41.0
17	38.054306526913415	40.0	37.0	41.0	33.0	41.0
18	37.9620372107854	40.0	37.0	41.0	33.0	41.0
19	37.86081157154688	40.0	36.0	41.0	33.0	41.0
20	37.757861826000706	40.0	36.0	41.0	33.0	41.0
21	37.64959585085775	40.0	36.0	41.0	33.0	41.0
22	37.62295262375965	39.0	36.0	41.0	33.0	41.0
23	37.575175690947596	39.0	35.0	41.0	33.0	41.0
24	37.518775404819316	39.0	35.0	41.0	33.0	41.0
25	37.36513806253907	39.0	35.0	41.0	32.0	41.0
26	37.21318673256459	39.0	35.0	41.0	32.0	41.0
27	37.15968864502457	39.0	35.0	41.0	32.0	41.0
28	37.03944986617718	39.0	35.0	41.0	32.0	41.0
29	36.98710588580547	39.0	35.0	41.0	31.0	41.0
30	36.79355308399163	39.0	35.0	41.0	31.0	41.0
31	36.65397525018589	39.0	35.0	41.0	30.0	41.0
32	36.53333436798523	39.0	35.0	40.0	30.0	41.0
33	36.344666063746786	39.0	35.0	40.0	30.0	41.0
34	36.16958885284851	39.0	35.0	40.0	30.0	41.0
35	35.983652029634314	39.0	35.0	40.0	29.0	41.0
36	35.83204213478787	38.0	35.0	40.0	28.0	41.0
37	35.73342664012314	38.0	35.0	40.0	27.0	41.0
38	35.54175173151347	38.0	34.0	40.0	27.0	41.0
39	35.49635779015284	38.0	35.0	40.0	26.0	41.0
40	35.32801757403287	38.0	34.0	40.0	25.0	41.0
41	35.21195079383667	38.0	34.0	40.0	25.0	41.0
42	35.15102860859542	38.0	34.0	40.0	25.0	41.0
43	35.04070555735052	38.0	34.0	40.0	24.0	41.0
44	34.85607380642321	38.0	34.0	40.0	24.0	41.0
45	34.71448122318429	38.0	34.0	40.0	23.0	41.0
46	34.63496069968805	38.0	33.0	40.0	23.0	41.0
47	34.4548000558712	37.0	33.0	40.0	23.0	41.0
48	34.337256074935134	37.0	33.0	40.0	23.0	41.0
49	34.22574505518692	37.0	33.0	40.0	23.0	41.0
50	34.04396753262328	37.0	33.0	40.0	22.0	41.0
51	32.094985277373716	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	16.0
10	32.0
11	28.0
12	20.0
13	12.0
14	33.0
15	52.0
16	119.0
17	205.0
18	374.0
19	710.0
20	1182.0
21	1931.0
22	2793.0
23	4182.0
24	5936.0
25	7880.0
26	9817.0
27	11017.0
28	12081.0
29	13670.0
30	16136.0
31	20224.0
32	26249.0
33	36815.0
34	51918.0
35	60442.0
36	71032.0
37	108023.0
38	128471.0
39	117340.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.452340876416	20.468048303194394	25.538501043352387	12.541109777037217
2	33.34706598586571	24.13904028511244	24.623257375774756	17.890636353247093
3	33.06672234975091	23.64819201634374	27.08427098662054	16.200814647284815
4	28.77874862614688	25.758176454238523	26.550108426816482	18.912966492798116
5	21.728254321200158	39.192378942200115	22.67481972366329	16.40454701293644
6	27.533356942208577	34.22788396284847	24.805685318148406	13.433073776794547
7	72.56596964049137	18.309529285116675	6.26378262151634	2.860718452875603
8	87.53733564907242	3.5114204406770573	6.531004990314247	2.4202389199362844
9	81.73477262818984	6.181104528530291	8.311546856327766	3.772575986952099
10	43.83434470556864	29.82788565591522	14.622594257964117	11.715175380552024
11	30.70912689958562	24.931536613273924	26.858246575419777	17.501089911720676
12	28.28225680154295	22.669881612307467	30.513577689895072	18.534283896254514
13	25.439174460652424	23.45969725144722	32.01405809758555	19.08707019031481
14	21.40219788282003	25.064019086505834	31.895120158358175	21.63866287231596
15	20.2174744241104	25.716978496641378	33.83043654315274	20.235110536095476
16	26.145324384534963	24.912912879017682	29.76439565276894	19.177367083678412
17	25.3665136792739	24.21931986686852	28.233440043568255	22.18072641028933
18	25.990973132441557	23.8880431393408	29.470507482649595	20.650476245568044
19	24.85591296508191	26.2615816347406	27.34697851075027	21.53552688942722
20	26.281616257955648	27.08032049753588	27.47748573943985	19.16057750506862
21	26.996372604486908	25.60438955772864	29.066005618159835	18.33323221962462
22	25.90349801699557	23.297586110080378	29.41971548013257	21.379200392791486
23	23.84472884830545	24.9848682159168	29.950350817539608	21.22005211823814
24	24.059466147835767	24.49585410279455	30.363318015782205	21.08136173358748
25	23.719441908763457	26.435685332257297	28.420947186193608	21.423925572785645
26	23.26795744194545	26.711937390391565	27.139013478222225	22.881091689440765
27	23.138861102214673	26.207685676514203	29.48856686132231	21.164886359948813
28	21.03790635365625	26.83285057416126	29.32165869749553	22.80758437468696
29	23.501600653523766	26.079859136846352	28.485989167194575	21.932551042435307
30	24.160626886182175	25.683117161630026	29.002515615013554	21.15374033717424
31	25.469508573266758	25.18845949267255	27.981314186629568	21.360717747431124
32	25.66787956087492	25.281719252849644	27.53617872012619	21.514222466149246
33	25.49716199685936	25.233607939354346	27.103600165356184	22.165629898430105
34	22.46403291321763	26.19992578724077	29.80926192165898	21.526779377882622
35	22.95093069290168	26.711655212599805	28.98233990290262	21.3550741915959
36	25.52904808732838	26.84837035270813	27.01922900561957	20.603352554343914
37	23.368977091395976	27.602631590085963	27.202644570264383	21.82574674825367
38	23.512746676298335	26.457977377806436	28.018561655142054	22.010714290753175
39	23.295046509954528	26.14207933992971	27.373503223175828	23.189370926939937
40	25.61990933627551	25.26972669669979	28.032247278042476	21.078116688982227
41	21.859043727681502	25.016189950802303	28.690709155117368	24.434057166398834
42	23.179917970915934	25.360587945646913	28.23132371013004	23.228170373307112
43	23.012022184817987	25.508731286321574	28.097289259043446	23.381957269816994
44	23.30040788799799	24.81330411852596	27.92614842834024	23.960139565135805
45	23.281784153741746	24.866212454481197	27.112488765796666	24.73951462598039
46	23.439098272648646	25.569963867133765	27.45335953824426	23.537578321973328
47	21.93128124237238	25.88148814923819	29.48616835009234	22.701062258297085
48	22.132191830106397	26.150544673682546	29.371321988845512	22.345941507365545
49	23.250180241064488	25.46160759509744	28.940013234138434	22.348198929699635
50	22.28160497084398	25.884168838259924	28.721607623315222	23.112618567580874
51	22.449218579150166	24.992204838502595	27.833735201538435	24.724841380808808
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	790.0
2	1290.0
3	4031.5
4	6773.0
5	4994.0
6	3215.0
7	2560.0
8	1905.0
9	1855.0
10	1805.0
11	1775.5
12	1746.0
13	1737.0
14	1728.0
15	1804.0
16	1880.0
17	1895.5
18	1911.0
19	1990.5
20	2070.0
21	2146.5
22	2223.0
23	2852.0
24	3481.0
25	4413.0
26	6118.5
27	6892.0
28	8298.0
29	9704.0
30	11109.0
31	12514.0
32	14891.0
33	17268.0
34	19471.5
35	21675.0
36	22590.5
37	23506.0
38	25557.0
39	27608.0
40	30292.5
41	32977.0
42	35448.5
43	37920.0
44	40331.5
45	42743.0
46	44891.0
47	47039.0
48	49646.5
49	52254.0
50	52768.5
51	53283.0
52	50414.5
53	47546.0
54	45027.5
55	42509.0
56	41062.5
57	39616.0
58	38884.5
59	38153.0
60	34614.5
61	31076.0
62	28528.5
63	25981.0
64	23138.5
65	20296.0
66	16974.0
67	13652.0
68	11941.0
69	10230.0
70	8611.0
71	6992.0
72	5768.0
73	4544.0
74	3764.5
75	2323.5
76	1662.0
77	1389.5
78	1117.0
79	834.0
80	551.0
81	446.5
82	342.0
83	287.5
84	233.0
85	163.0
86	93.0
87	80.0
88	67.0
89	56.0
90	45.0
91	33.0
92	21.0
93	13.5
94	6.0
95	6.5
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	708773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35382374604215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.57672420935417	26.747952637180138
2	8.214764043238095	5.9727616828640455
3	3.861790604985339	4.211725649932755
4	2.4959308446284645	3.6294652003173327
5	1.8005285306132015	3.2728048425816296
6	1.3121656180220447	2.862134256191393
7	0.9940931524151854	2.5297361075034344
8	0.7914387777331608	2.3017460665195486
9	0.6717813756535457	2.1979639553744454
>10	6.20168059480126	42.65168017678045
>50	0.05621479606073487	1.3461635229099564
>100	0.020478246968982275	1.2318666118702633
>500	0.0020076712714688504	0.5617043159707044
>1k	4.015342542937701E-4	0.48229497400395194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3304	0.46615771198959327	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	940	0.13262356212778986	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	899	0.12683891739668413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04416082441063641	0.0
2	1.410888958806275E-4	0.0	0.0	0.17311607524552994	0.0
3	1.410888958806275E-4	0.0	0.0	0.2699030578196404	0.0
4	1.410888958806275E-4	0.0	0.0	0.38954644152641255	0.0
5	1.410888958806275E-4	0.0	0.0	0.77796417188578	0.0
6	1.410888958806275E-4	0.0	0.0	1.1267359225026912	0.0
7	1.410888958806275E-4	0.0	0.0	1.410888958806275	0.0
8	1.410888958806275E-4	0.0	0.0	1.872249648335927	0.0
9	1.410888958806275E-4	0.0	0.0	2.1046230598513205	0.0
10	1.410888958806275E-4	0.0	0.0	2.5570951489404927	0.0
11	1.410888958806275E-4	0.0	0.0	3.192277358195078	0.0
12	1.410888958806275E-4	0.0	0.0	3.640657869303712	0.0
13	1.410888958806275E-4	0.0	0.0	3.8553951688340273	0.0
14	1.410888958806275E-4	0.0	0.0	3.9719345968314257	0.0
15	1.410888958806275E-4	0.0	0.0	4.141664538575821	0.0
16	1.410888958806275E-4	0.0	0.0	4.450508131658514	0.0
17	1.410888958806275E-4	0.0	0.0	4.826792216972148	0.0
18	1.410888958806275E-4	0.0	0.0	5.226073792314324	0.0
19	1.410888958806275E-4	0.0	0.0	5.490192205402858	0.0
20	1.410888958806275E-4	0.0	0.0	5.788736309086266	0.0
21	1.410888958806275E-4	0.0	0.0	6.120436303301621	0.0
22	1.410888958806275E-4	0.0	0.0	6.4772501209837285	0.0
23	1.410888958806275E-4	0.0	0.0	6.834769383145239	0.0
24	1.410888958806275E-4	0.0	0.0	7.126964486514018	0.0
25	1.410888958806275E-4	0.0	0.0	7.404345255815332	0.0
26	1.410888958806275E-4	0.0	0.0	7.652661712565236	0.0
27	1.410888958806275E-4	0.0	0.0	7.935121682118252	0.0
28	1.410888958806275E-4	0.0	0.0	8.211655918044283	0.0
29	1.410888958806275E-4	0.0	0.0	8.519794066647572	0.0
30	1.410888958806275E-4	0.0	0.0	8.982706734031911	0.0
31	1.410888958806275E-4	0.0	0.0	9.313842372663744	0.0
32	1.410888958806275E-4	0.0	0.0	9.637500299813905	0.0
33	1.410888958806275E-4	0.0	0.0	9.947472604063643	0.0
34	1.410888958806275E-4	0.0	0.0	10.287355754240075	0.0
35	1.410888958806275E-4	0.0	0.0	10.65065966113269	0.0
36	1.410888958806275E-4	0.0	0.0	10.972624521532282	0.0
37	1.410888958806275E-4	0.0	0.0	11.323653694483284	0.0
38	1.410888958806275E-4	0.0	0.0	11.70036104648456	0.0
39	1.410888958806275E-4	0.0	0.0	12.264293363319426	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	7.0314534E-4	45.000004	1
AATCCGG	20	7.0314534E-4	45.000004	2
CTAACGG	35	1.2110468E-7	45.000004	2
CTACGAA	125	0.0	41.399998	11
CGAATAT	125	0.0	41.399998	14
CGTTTTT	1150	0.0	41.282608	1
GCGATAT	55	6.002665E-11	40.909092	9
ACGCTAG	45	1.9264917E-8	40.0	1
TACGAAT	130	0.0	39.807693	12
ATGGGCG	125	0.0	39.6	5
CGTTATT	245	0.0	39.489796	1
TTACGGG	120	0.0	39.375004	3
CACGACC	195	0.0	38.076923	27
ATTACGG	30	1.1394803E-4	37.500004	2
TACTATC	30	1.1394803E-4	37.500004	40
TATAGCG	30	1.1394803E-4	37.500004	1
ACCCGTG	30	1.1394803E-4	37.500004	37
TATGGGA	475	0.0	37.42105	4
AAACACG	230	0.0	37.173912	40
ACGACCA	200	0.0	37.125	28
>>END_MODULE