FastQCFastQC Report
Sat 18 Jun 2016
SRR3551116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026217
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47260.4605263798982086No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.13807995774772783No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.032932E-445.0000041
CGTTATT4250.043.4117661
GTTAACG556.184564E-1140.9090921
ACTACGG556.184564E-1140.9090922
CGTTTTT19350.039.767441
ACACGAC5900.039.27966326
CGTTTTA3250.038.769231
TACGAAT700.038.57142612
TCGAACG356.2476356E-638.5714261
TCAAGCG6400.038.32031617
CACGACC6050.038.30578627
CACGTGA6050.037.93388743
TACGGGA4700.037.819154
ACGACCA6150.037.6829328
GGTACGA301.13983646E-437.5000048
TGGACGA601.546141E-1037.5000045
GCGAGAC6300.037.521
GACACGA6250.037.4425
AACGAGC3750.037.215
CGGTTTT5450.037.155961
Produced by FastQC (version 0.11.5)