##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026217
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94124537013127	33.0	31.0	34.0	30.0	34.0
2	32.070513351464655	33.0	31.0	34.0	30.0	34.0
3	31.873044395093824	33.0	31.0	34.0	30.0	34.0
4	35.668718214568656	37.0	35.0	37.0	33.0	37.0
5	31.10050310996602	37.0	35.0	37.0	0.0	37.0
6	33.37832154407889	37.0	35.0	37.0	17.0	37.0
7	29.630577158632143	35.0	32.0	37.0	0.0	37.0
8	32.71166137376403	35.0	35.0	37.0	17.0	37.0
9	36.61047127459397	39.0	35.0	39.0	32.0	39.0
10	36.87426440996398	38.0	37.0	39.0	33.0	39.0
11	36.967393835806654	39.0	37.0	39.0	33.0	39.0
12	37.02040893885017	39.0	37.0	39.0	33.0	39.0
13	36.92569895061181	39.0	37.0	39.0	33.0	39.0
14	38.19808481052253	40.0	37.0	41.0	33.0	41.0
15	38.27425778368513	40.0	37.0	41.0	33.0	41.0
16	38.31861487385222	40.0	37.0	41.0	34.0	41.0
17	38.25696124698772	40.0	37.0	41.0	34.0	41.0
18	38.16164417467261	40.0	37.0	41.0	33.0	41.0
19	38.05617525338208	40.0	37.0	41.0	34.0	41.0
20	37.96136781986656	40.0	37.0	41.0	33.0	41.0
21	37.86106447271873	40.0	36.0	41.0	33.0	41.0
22	37.82648016939887	40.0	36.0	41.0	33.0	41.0
23	37.773819767164255	40.0	36.0	41.0	33.0	41.0
24	37.7339870612161	40.0	36.0	41.0	33.0	41.0
25	37.58191103830866	39.0	36.0	41.0	33.0	41.0
26	37.42445506164876	39.0	35.0	41.0	33.0	41.0
27	37.36871831201393	39.0	35.0	41.0	33.0	41.0
28	37.194760952118315	39.0	35.0	41.0	32.0	41.0
29	37.130270693235445	39.0	35.0	41.0	32.0	41.0
30	37.00978448028049	39.0	35.0	41.0	31.0	41.0
31	36.894798078768915	39.0	35.0	41.0	31.0	41.0
32	36.77955637063116	39.0	35.0	41.0	31.0	41.0
33	36.562879001224886	39.0	35.0	41.0	30.0	41.0
34	36.363061613674304	39.0	35.0	41.0	30.0	41.0
35	36.228939883085154	39.0	35.0	41.0	29.0	41.0
36	36.09003358938704	39.0	35.0	40.0	29.0	41.0
37	36.00519383327308	39.0	35.0	40.0	29.0	41.0
38	35.831417721593	39.0	35.0	40.0	28.0	41.0
39	35.76782785707117	39.0	35.0	40.0	27.0	41.0
40	35.612683282385696	38.0	35.0	40.0	26.0	41.0
41	35.499000698682636	38.0	35.0	40.0	26.0	41.0
42	35.453576582730555	38.0	35.0	40.0	26.0	41.0
43	35.30074341001952	38.0	34.0	40.0	25.0	41.0
44	35.05300048625193	38.0	34.0	40.0	24.0	41.0
45	34.93148525117007	38.0	34.0	40.0	24.0	41.0
46	34.7938681584889	38.0	34.0	40.0	23.0	41.0
47	34.67426285083954	38.0	34.0	40.0	23.0	41.0
48	34.596302731293676	38.0	34.0	40.0	23.0	41.0
49	34.421772393168304	37.0	33.0	40.0	23.0	41.0
50	34.239182356168335	37.0	33.0	40.0	22.0	41.0
51	32.29017936752168	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	18.0
9	34.0
10	38.0
11	32.0
12	15.0
13	21.0
14	18.0
15	64.0
16	122.0
17	261.0
18	480.0
19	933.0
20	1674.0
21	2621.0
22	3951.0
23	5658.0
24	7870.0
25	10785.0
26	13466.0
27	14826.0
28	16179.0
29	18086.0
30	21707.0
31	27056.0
32	34848.0
33	48985.0
34	70981.0
35	85293.0
36	104061.0
37	159805.0
38	194378.0
39	181923.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85108997414777	20.499270622100394	24.462272599265066	13.18736680448677
2	33.27288477972982	23.116163540459766	25.868115612974645	17.742836066835764
3	32.059593633705155	23.73036112245266	27.599718188258425	16.61032705558376
4	27.174661889249546	26.25468102750198	27.581398476150753	18.98925860709772
5	21.705935489277607	39.3602912444444	22.867775528957328	16.065997737320664
6	26.357290904360386	35.116354533203015	24.821358445630896	13.704996116805704
7	72.49363438726897	18.07785292974098	6.644598559563914	2.7839141234261366
8	87.80423633597962	3.1577142066444033	6.7669898276875164	2.2710596296884575
9	82.3509062898003	5.58975343421518	8.360804781055078	3.698535494929435
10	40.78932623412007	31.51886979069729	15.025769403547203	12.666034571635434
11	27.716262739751922	26.37931353700046	26.97129359579894	18.933130127448678
12	25.188629695278873	23.881888528449636	31.272040903629545	19.657440872641946
13	23.08069345957044	24.379444113671862	32.49390723404504	20.045955192712654
14	20.587263707383528	26.471594214479005	32.11806080000624	20.823081278131234
15	19.69232628186826	26.222134304927707	33.948472886338855	20.137066526865173
16	24.114198069219277	26.06622186145815	30.975612370483045	18.843967698839524
17	24.458179897623992	24.488680269377724	29.073090779045756	21.980049053952527
18	24.971619063024683	24.571411309693758	29.57795476005562	20.879014867225937
19	24.756265000482355	26.912826429497855	27.49818020944888	20.83272836057091
20	26.621854831872792	26.409131791814012	27.796265312307238	19.172748064005958
21	25.903001022200954	26.369471564006442	28.936667391009895	18.790860022782706
22	25.527544369270828	23.717400900589254	28.836785981912204	21.918268748227714
23	24.317956143778556	25.680143673316657	28.847310071846405	21.154590111058383
24	22.6221159852156	24.832856988336776	30.567024323315632	21.97800270313199
25	23.409278934182538	26.191731378451145	28.710496902701866	21.68849278466445
26	22.660801760251488	27.804937941975233	27.698527699307263	21.835732598466016
27	22.67717256681579	26.704975653297495	29.041226173411665	21.576625606475044
28	21.18148500755688	28.113742025322132	29.195287156615024	21.509485810505964
29	22.569105754435952	25.847846995323604	29.465308019648866	22.117739230591578
30	23.324014316660122	25.178787722284856	29.934019802829226	21.563178158225792
31	23.709800168970112	26.43690369580703	28.47156108308477	21.381735052138097
32	23.879549841797594	26.061252152322563	28.772374653703846	21.286823352176
33	23.303940589563414	26.009703600700433	28.120465749446755	22.56589006028939
34	21.95812386658962	26.188515684304587	30.128715466611837	21.724644982493956
35	22.192089977071124	26.570306280250666	28.8936940237786	22.34390971889961
36	22.927022257475755	27.777458373813722	27.801040130888495	21.49447923782202
37	22.649498108099944	26.566993140826938	28.44008625855935	22.343422492513767
38	22.561407577539644	26.511449332840908	28.077394936938287	22.84974815268116
39	23.645193950207414	25.779148074919828	27.15137246800628	23.424285506866482
40	24.223531670202306	25.38352024961582	28.561405628634102	21.831542451547772
41	20.877845523899914	25.99138388859276	28.868553142269132	24.26221744523819
42	22.227267722128946	26.195044517874877	28.41260669039784	23.16508106959834
43	22.37499476231635	25.31784213280427	28.51784758973979	23.789315515139585
44	22.40198710409202	26.122740122215866	28.171429629405864	23.303843144286247
45	22.925365687763893	25.219812183972785	27.435620341506716	24.419201786756602
46	22.3705122795666	26.12312990332454	27.73341310853358	23.772944708575285
47	21.4664149979975	26.051799960437215	29.314755066423576	23.16702997514171
48	22.270046198805908	25.55794729574739	29.14295904277555	23.02904746267115
49	22.955671168963292	25.349511847884028	28.90928526812555	22.785531715027133
50	21.575358817871855	26.110949243678483	29.077183480686834	23.23650845776283
51	21.87646472432244	25.072962151279892	28.149991668428804	24.900581455968865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	352.0
1	734.0
2	1116.0
3	5567.5
4	10019.0
5	7719.0
6	5419.0
7	4356.5
8	3294.0
9	3200.5
10	3107.0
11	3083.5
12	3060.0
13	3110.5
14	3161.0
15	3084.5
16	3008.0
17	2957.5
18	2907.0
19	2864.0
20	2821.0
21	3371.0
22	3921.0
23	4523.0
24	5125.0
25	6164.5
26	8510.5
27	9817.0
28	11831.0
29	13845.0
30	16375.5
31	18906.0
32	22461.0
33	26016.0
34	29262.5
35	32509.0
36	34842.5
37	37176.0
38	40784.0
39	44392.0
40	47117.5
41	49843.0
42	54201.5
43	58560.0
44	62339.0
45	66118.0
46	71630.0
47	77142.0
48	78135.0
49	79128.0
50	77285.0
51	75442.0
52	71310.0
53	67178.0
54	62690.5
55	58203.0
56	54355.0
57	50507.0
58	48111.0
59	45715.0
60	44495.5
61	43276.0
62	39290.5
63	35305.0
64	30752.0
65	26199.0
66	22051.0
67	17903.0
68	15467.0
69	13031.0
70	11062.5
71	9094.0
72	7766.0
73	6438.0
74	5463.5
75	3523.5
76	2558.0
77	1939.5
78	1321.0
79	1019.0
80	717.0
81	568.5
82	420.0
83	338.5
84	257.0
85	170.0
86	83.0
87	69.0
88	55.0
89	40.5
90	26.0
91	21.0
92	16.0
93	15.5
94	15.0
95	9.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1026217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.91512453173412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64755205266023	24.638507747916087
2	8.491164879335555	5.75957828604305
3	3.951510741075105	4.020479366161417
4	2.5521455691690584	3.4622533920592833
5	1.8697980386543123	3.1707216665076614
6	1.4088627931406892	2.866905424649595
7	1.0977322242376983	2.6060807561263317
8	0.8510766113084168	2.309149540685699
9	0.6886206271817629	2.101918889339131
>10	6.279397193001772	44.265843422741476
>50	0.14354297361634435	2.925690871060057
>100	0.01743402808054871	1.1134735766909936
>500	5.811342692514213E-4	0.15402771928496786
>1k	5.811342692514213E-4	0.6053693407342019
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4726	0.4605263798982086	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1417	0.13807995774772783	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9489055433694822E-4	0.0	0.0	0.051938332730796705	0.0
2	1.9489055433694822E-4	0.0	0.0	0.1930390940707472	0.0
3	1.9489055433694822E-4	0.0	0.0	0.2983774386898677	0.0
4	1.9489055433694822E-4	0.0	0.0	0.42632308761207427	0.0
5	1.9489055433694822E-4	0.0	0.0	0.824776825953965	0.0
6	1.9489055433694822E-4	0.0	0.0	1.2221586662470023	0.0
7	1.9489055433694822E-4	0.0	0.0	1.5329116551372663	0.0
8	1.9489055433694822E-4	0.0	0.0	2.0494690694073476	0.0
9	1.9489055433694822E-4	0.0	0.0	2.30477569558875	0.0
10	1.9489055433694822E-4	0.0	0.0	2.7916123003224462	0.0
11	1.9489055433694822E-4	0.0	0.0	3.3455887010252217	0.0
12	1.9489055433694822E-4	0.0	0.0	3.7931548590600235	0.0
13	1.9489055433694822E-4	0.0	0.0	4.016304543775829	0.0
14	1.9489055433694822E-4	0.0	0.0	4.133336321655166	0.0
15	1.9489055433694822E-4	0.0	0.0	4.270149490799704	0.0
16	1.9489055433694822E-4	0.0	0.0	4.570086053924268	0.0
17	1.9489055433694822E-4	0.0	0.0	4.950122634881317	0.0
18	1.9489055433694822E-4	0.0	0.0	5.357541338722707	0.0
19	1.9489055433694822E-4	0.0	0.0	5.628244318696728	0.0
20	1.9489055433694822E-4	0.0	0.0	5.88803342762788	0.0
21	1.9489055433694822E-4	0.0	0.0	6.215547004191121	0.0
22	1.9489055433694822E-4	0.0	0.0	6.583987597165122	0.0
23	1.9489055433694822E-4	0.0	0.0	6.954279650405324	0.0
24	1.9489055433694822E-4	0.0	0.0	7.240573874726301	0.0
25	1.9489055433694822E-4	0.0	0.0	7.50240933447799	0.0
26	1.9489055433694822E-4	0.0	0.0	7.739298803274551	0.0
27	1.9489055433694822E-4	0.0	0.0	7.987686814776992	0.0
28	1.9489055433694822E-4	0.0	0.0	8.270180673288397	0.0
29	1.9489055433694822E-4	0.0	0.0	8.53766795911586	0.0
30	1.9489055433694822E-4	0.0	0.0	8.883403802509605	0.0
31	1.9489055433694822E-4	0.0	0.0	9.192305331133669	0.0
32	1.9489055433694822E-4	0.0	0.0	9.51855211909372	0.0
33	1.9489055433694822E-4	0.0	0.0	9.826089413837424	0.0
34	1.9489055433694822E-4	0.0	0.0	10.124271961972955	0.0
35	1.9489055433694822E-4	0.0	0.0	10.450518749933007	0.0
36	1.9489055433694822E-4	0.0	0.0	10.747434509465347	0.0
37	1.9489055433694822E-4	0.0	0.0	11.056141147535072	0.0
38	1.9489055433694822E-4	0.0	0.0	11.3972970629019	0.0
39	1.9489055433694822E-4	0.0	0.0	11.779185104125151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.032932E-4	45.000004	1
CGTTATT	425	0.0	43.411766	1
GTTAACG	55	6.184564E-11	40.909092	1
ACTACGG	55	6.184564E-11	40.909092	2
CGTTTTT	1935	0.0	39.76744	1
ACACGAC	590	0.0	39.279663	26
CGTTTTA	325	0.0	38.76923	1
TACGAAT	70	0.0	38.571426	12
TCGAACG	35	6.2476356E-6	38.571426	1
TCAAGCG	640	0.0	38.320316	17
CACGACC	605	0.0	38.305786	27
CACGTGA	605	0.0	37.933887	43
TACGGGA	470	0.0	37.81915	4
ACGACCA	615	0.0	37.68293	28
GGTACGA	30	1.13983646E-4	37.500004	8
TGGACGA	60	1.546141E-10	37.500004	5
GCGAGAC	630	0.0	37.5	21
GACACGA	625	0.0	37.44	25
AACGAGC	375	0.0	37.2	15
CGGTTTT	545	0.0	37.15596	1
>>END_MODULE