FastQCFastQC Report
Sat 18 Jun 2016
SRR3551115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267966
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7320.27316898412485163No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.12986722196099504No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC2950.11008859332900442No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG253.8827733E-545.0000042
TGGAACG253.8827733E-545.0000041
CACGACC502.1827873E-1145.00000427
AGACACG502.1827873E-1145.00000424
TTAACGG253.8827733E-545.0000042
ACGACCA502.1827873E-1145.00000428
TCCGCGT207.023594E-445.016
GTATTAG207.023594E-445.01
AATCTCG207.023594E-445.018
AGCGAAG207.023594E-445.01
CTCGCGA207.023594E-445.026
TGAGCGC351.2072633E-745.021
CTCTAAT207.023594E-445.044
TTAGGTT302.1592514E-644.99999612
AGTACGG302.1592514E-644.9999962
TACTCCT1500.042.022
GGCGATA700.041.7857138
ACTATGC1750.041.142868
CCTCGTG1750.041.1428615
CCCTCGT1700.041.0294114
Produced by FastQC (version 0.11.5)