##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	267966
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.877294134330473	31.0	31.0	34.0	30.0	34.0
2	32.02089444183217	33.0	31.0	34.0	30.0	34.0
3	31.961551092302756	33.0	31.0	34.0	30.0	34.0
4	35.68928147600815	37.0	35.0	37.0	35.0	37.0
5	31.265977773299596	37.0	35.0	37.0	0.0	37.0
6	33.448922624512065	37.0	35.0	37.0	17.0	37.0
7	29.54275915601233	35.0	32.0	37.0	0.0	37.0
8	32.65110125911496	35.0	35.0	37.0	17.0	37.0
9	36.54614018196338	39.0	35.0	39.0	32.0	39.0
10	36.85696319682348	38.0	37.0	39.0	33.0	39.0
11	36.91410850630304	39.0	37.0	39.0	33.0	39.0
12	36.84340923848548	39.0	35.0	39.0	33.0	39.0
13	36.51755073404835	39.0	35.0	39.0	32.0	39.0
14	37.71828142376272	40.0	36.0	41.0	33.0	41.0
15	37.87505131247994	40.0	36.0	41.0	33.0	41.0
16	37.95976355209243	40.0	36.0	41.0	33.0	41.0
17	37.93851458767157	40.0	36.0	41.0	33.0	41.0
18	37.849118918071696	40.0	36.0	41.0	33.0	41.0
19	37.76517170088743	39.0	36.0	41.0	33.0	41.0
20	37.684403991551164	39.0	36.0	41.0	33.0	41.0
21	37.55789167282416	39.0	35.0	41.0	33.0	41.0
22	37.50558652963436	39.0	35.0	41.0	33.0	41.0
23	37.463868550487746	39.0	35.0	41.0	33.0	41.0
24	37.42620705611906	39.0	35.0	41.0	33.0	41.0
25	37.2865848652441	39.0	35.0	41.0	32.0	41.0
26	37.097807184493554	39.0	35.0	41.0	32.0	41.0
27	37.04956598971511	39.0	35.0	41.0	32.0	41.0
28	36.866583820335414	39.0	35.0	40.0	31.0	41.0
29	36.918642663621505	39.0	35.0	40.0	31.0	41.0
30	36.8337811513401	39.0	35.0	40.0	31.0	41.0
31	36.68816193099124	39.0	35.0	40.0	31.0	41.0
32	36.59315734085668	39.0	35.0	40.0	31.0	41.0
33	36.45464349954845	39.0	35.0	40.0	30.0	41.0
34	36.34458476075323	39.0	35.0	40.0	30.0	41.0
35	36.266776382078326	38.0	35.0	40.0	30.0	41.0
36	36.139812513527836	38.0	35.0	40.0	30.0	41.0
37	35.97561630953181	38.0	35.0	40.0	29.0	41.0
38	35.90249136084429	38.0	35.0	40.0	29.0	41.0
39	35.861586917743296	38.0	35.0	40.0	29.0	41.0
40	35.69406939686378	38.0	35.0	40.0	28.0	41.0
41	35.63827127322123	38.0	35.0	40.0	28.0	41.0
42	35.52164080517677	38.0	34.0	40.0	28.0	41.0
43	35.360609181761866	38.0	34.0	40.0	27.0	41.0
44	35.167644402648094	38.0	34.0	40.0	26.0	41.0
45	35.01561018935238	38.0	34.0	40.0	26.0	41.0
46	34.84356970660457	37.0	34.0	40.0	25.0	41.0
47	34.665200062694524	37.0	33.0	40.0	24.0	41.0
48	34.598993902211475	37.0	34.0	40.0	24.0	41.0
49	34.417892568460175	37.0	33.0	40.0	24.0	41.0
50	34.24643798093788	37.0	33.0	40.0	23.0	41.0
51	32.02806326175709	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	8.0
11	5.0
12	7.0
13	7.0
14	7.0
15	9.0
16	29.0
17	48.0
18	105.0
19	191.0
20	356.0
21	593.0
22	899.0
23	1307.0
24	1800.0
25	2376.0
26	2957.0
27	3439.0
28	4072.0
29	5023.0
30	6243.0
31	7983.0
32	10208.0
33	15031.0
34	22042.0
35	26852.0
36	26936.0
37	40009.0
38	47281.0
39	42132.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69658837315182	19.32558608181635	25.02705567124187	11.950769873789959
2	32.24364285021234	23.1555495846488	27.096348044154855	17.504459520984007
3	31.892852078248733	23.66904756573595	27.570661949650326	16.867438406364986
4	28.802161468245973	26.04808072665935	26.170857496846615	18.97890030824806
5	21.62102654814417	39.75056537023354	22.258047662763186	16.370360418859107
6	27.173596650321308	35.481367039101976	23.814215236261315	13.5308210743154
7	71.35382847077615	19.282297007829353	5.698110954374809	3.6657635670196966
8	86.10084861512281	4.190456998275901	6.47992655784689	3.228767828754394
9	80.30906906100027	6.242583014263003	8.318965838949717	5.129382085787003
10	44.21083271758358	28.324488927699782	14.625736100848616	12.838942253868026
11	31.776419396490603	24.899054357642385	25.0557906600091	18.268735585857907
12	29.453363486412453	22.01659912078398	28.80253464991827	19.727502742885292
13	23.637327123590307	26.04808072665935	31.538702671234407	18.775889478515932
14	19.881253591873595	29.656747497816887	30.089638237686874	20.372360672622648
15	19.59651597590739	23.99558152899995	36.76809744519827	19.63980504989439
16	23.261159997910184	24.397498190068887	32.51121410925267	19.83012770276826
17	22.741691110066203	23.250337729413435	28.141256726599646	25.866714433920723
18	25.313286013897283	24.071337408477195	30.092996872737587	20.522379704887932
19	26.161901136711375	26.252584283080687	26.248852466357675	21.336662113850263
20	25.89656896770486	26.386556503437003	28.22372987617832	19.493144652679817
21	24.459446347670973	25.796556279527998	29.69705111842547	20.046946254375555
22	23.161520491405625	23.863475217005142	28.70550741511983	24.269496876469404
23	22.434935775434198	26.613450960196445	27.249725711470855	23.7018875528985
24	23.83697931827172	23.62874394512737	31.24836733018368	21.285909406417233
25	22.539053462006372	25.696916773023442	29.96648828582731	21.797541479142875
26	22.303202645111693	27.981908152526813	27.841218662069068	21.873670540292427
27	23.17943321167611	26.007030742706167	29.137651791645208	21.67588425397252
28	19.433435585111543	26.556354164334284	33.15644522066232	20.853765029891854
29	22.195726323488802	23.571273967592905	32.17423105916422	22.058768649754075
30	23.658598478911504	25.278953300045526	29.164894053723234	21.897554167319736
31	24.505720875036385	27.43109200420949	26.561578707746502	21.50160841300762
32	24.045961054760678	28.00317950784801	27.524387422284917	20.426472015106395
33	23.874670667174193	26.482837374890845	26.665696394318683	22.976795563616278
34	21.594157467738444	25.132666084503256	29.970593284222623	23.302583163535672
35	22.231551764029764	23.861982490315935	31.24165006008225	22.66481568557205
36	24.710597613130027	24.828896203249666	29.3977594172395	21.062746766380812
37	22.2610331161416	26.648903219065108	30.347133591575048	20.742930073218243
38	22.207294955330152	27.55125650269064	27.64082010404305	22.60062843793616
39	23.3309449706306	25.696916773023442	28.440548427785618	22.53158982856034
40	24.652008090578654	23.81906659800124	29.008530933028815	22.52039437839129
41	21.75499876850048	25.238649679436943	28.036392676682865	24.969958875379714
42	23.691811647746356	25.584215907988327	28.24985259323944	22.474119851025876
43	23.277206809819155	23.9198256495227	29.674660218087368	23.128307322570773
44	21.541538851943905	24.830388929938874	29.680631124844197	23.947441093273028
45	23.878775665569513	25.35172372614436	27.801288223132786	22.968212385153343
46	24.933387071494145	25.495025488308222	27.14822029660479	22.42336714359284
47	21.79791466081518	26.136151601322556	29.78848062813939	22.277453109722874
48	22.825283804661787	24.038497421314645	29.651896136076967	23.484322637946605
49	22.0647395565109	22.806997902719	31.430479986266914	23.69778255450318
50	20.438040646947748	25.007650224282184	31.06662785577275	23.48768127299732
51	21.88785144383989	24.947941156713913	27.044102610032617	26.120104789413585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	186.0
1	441.0
2	696.0
3	842.5
4	989.0
5	792.5
6	596.0
7	534.0
8	472.0
9	510.0
10	548.0
11	609.0
12	670.0
13	637.5
14	605.0
15	666.0
16	727.0
17	792.5
18	858.0
19	808.0
20	758.0
21	820.5
22	883.0
23	974.0
24	1065.0
25	1274.0
26	1775.0
27	2067.0
28	2581.5
29	3096.0
30	3895.5
31	4695.0
32	5271.5
33	5848.0
34	6506.5
35	7165.0
36	7989.5
37	8814.0
38	9274.0
39	9734.0
40	11262.5
41	12791.0
42	14925.0
43	17059.0
44	18491.5
45	19924.0
46	21923.0
47	23922.0
48	25314.0
49	26706.0
50	25519.5
51	24333.0
52	21544.5
53	18756.0
54	16418.0
55	14080.0
56	13023.0
57	11966.0
58	11146.0
59	10326.0
60	9912.5
61	9499.0
62	8852.0
63	8205.0
64	7164.0
65	6123.0
66	5104.0
67	4085.0
68	3422.0
69	2759.0
70	2366.0
71	1973.0
72	1652.5
73	1332.0
74	1077.5
75	698.5
76	574.0
77	426.0
78	278.0
79	257.5
80	237.0
81	187.5
82	138.0
83	101.0
84	64.0
85	52.0
86	40.0
87	25.0
88	10.0
89	7.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	267966.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.90564228234673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9074448231717	27.25697880904732
2	9.764718155248291	7.402758267615568
3	4.341388932843518	4.936894076907163
4	2.6701538314913598	4.048555839013961
5	1.8973796211681617	3.5960696596907455
6	1.3645530523044596	3.103455592556233
7	0.9456902161547447	2.5092896530433837
8	0.762750141195381	2.313002720236926
9	0.5998035185689564	2.0462343853111
>10	5.683138338404106	40.41350804192576
>50	0.04898395401614622	1.21372013631645
>100	0.012995742902242874	0.8821543794394929
>500	9.996725309417596E-4	0.277378438895923
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	732	0.27316898412485163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.12986722196099504	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	295	0.11008859332900442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16681220751886433	0.0
2	0.0	0.0	0.0	0.6418724763589411	0.0
3	0.0	0.0	0.0	0.8590642096385362	0.0
4	0.0	0.0	0.0	1.1105886567698888	0.0
5	0.0	0.0	0.0	1.9028533470664188	0.0
6	0.0	0.0	0.0	2.3260413634565578	0.0
7	0.0	0.0	0.0	2.8022211773135397	0.0
8	0.0	0.0	0.0	3.6579267519013605	0.0
9	0.0	0.0	0.0	3.9568452714150304	0.0
10	0.0	0.0	0.0	4.808819029279834	0.0
11	0.0	0.0	0.0	5.935081316286395	0.0
12	0.0	0.0	0.0	6.8176559712799385	0.0
13	0.0	0.0	0.0	7.1803885567572	0.0
14	0.0	0.0	0.0	7.3005530552383515	0.0
15	0.0	0.0	0.0	7.584917489532254	0.0
16	0.0	0.0	0.0	8.168200443339826	0.0
17	0.0	0.0	0.0	8.92837150981841	0.0
18	0.0	0.0	0.0	9.662419859235873	0.0
19	0.0	0.0	0.0	10.079636968869185	0.0
20	0.0	0.0	0.0	10.511408163722264	0.0
21	0.0	0.0	0.0	11.08909339244531	0.0
22	0.0	0.0	0.0	11.708574968466149	0.0
23	0.0	0.0	0.0	12.29820201070285	0.0
24	0.0	0.0	0.0	12.77587455124904	0.0
25	0.0	0.0	0.0	13.211377562825135	0.0
26	0.0	0.0	0.0	13.614413768910982	0.0
27	0.0	0.0	0.0	14.007747251516983	0.0
28	0.0	0.0	0.0	14.409663912585925	0.0
29	0.0	0.0	0.0	14.80560966689804	0.0
30	0.0	0.0	0.0	15.299702201025504	0.0
31	0.0	0.0	0.0	15.742668846047632	0.0
32	0.0	0.0	0.0	16.171454587522298	0.0
33	0.0	0.0	0.0	16.618526230939747	0.0
34	0.0	0.0	0.0	17.028279707127023	0.0
35	0.0	0.0	0.0	17.49662270586567	0.0
36	0.0	0.0	0.0	17.940708895904706	0.0
37	0.0	0.0	0.0	18.3538210071427	0.0
38	0.0	0.0	0.0	18.774023570154423	0.0
39	0.0	0.0	0.0	19.206914310024406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	25	3.8827733E-5	45.000004	2
TGGAACG	25	3.8827733E-5	45.000004	1
CACGACC	50	2.1827873E-11	45.000004	27
AGACACG	50	2.1827873E-11	45.000004	24
TTAACGG	25	3.8827733E-5	45.000004	2
ACGACCA	50	2.1827873E-11	45.000004	28
TCCGCGT	20	7.023594E-4	45.0	16
GTATTAG	20	7.023594E-4	45.0	1
AATCTCG	20	7.023594E-4	45.0	18
AGCGAAG	20	7.023594E-4	45.0	1
CTCGCGA	20	7.023594E-4	45.0	26
TGAGCGC	35	1.2072633E-7	45.0	21
CTCTAAT	20	7.023594E-4	45.0	44
TTAGGTT	30	2.1592514E-6	44.999996	12
AGTACGG	30	2.1592514E-6	44.999996	2
TACTCCT	150	0.0	42.0	22
GGCGATA	70	0.0	41.785713	8
ACTATGC	175	0.0	41.14286	8
CCTCGTG	175	0.0	41.14286	15
CCCTCGT	170	0.0	41.02941	14
>>END_MODULE