FastQCFastQC Report
Sat 18 Jun 2016
SRR3551108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178552
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.884336215780277No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9350.523656973878758No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG4050.22682467852502353No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.2189838254402079No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC3480.19490120525113133No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC3230.18089968188538913No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG2710.1517765132846454No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC2570.14393566019982976No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC2360.1321743805726063No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2300.12881401496482817No Hit
GAATCTATCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC2200.1232134056185313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAG253.8775353E-545.0000042
CACATCC253.8775353E-545.00000445
TGAAACG302.1551768E-645.0000044
TAGCATA253.8775353E-545.00000430
AAACGGC302.1551768E-645.0000046
TCGTCCC302.1551768E-645.00000438
ATCGGGG302.1551768E-645.0000044
CAATAAG253.8775353E-545.0000042
ACAAGGC253.8775353E-545.0000044
ACGACGG253.8775353E-545.0000042
AGGACCG351.2042256E-745.07
AACGGCT207.0172723E-445.07
AGGCCTA207.0172723E-445.01
CAAGGCG207.0172723E-445.05
GCGATGT551.8189894E-1245.09
AACCTGC207.0172723E-445.033
CAATCAT207.0172723E-445.012
TAGTATC207.0172723E-445.038
CGTTTTA2750.044.181821
CTATCTC900.042.55