##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178552
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.802292889466376	31.0	31.0	34.0	30.0	34.0
2	31.971957749003092	33.0	31.0	34.0	30.0	34.0
3	31.666522917693445	31.0	31.0	34.0	30.0	34.0
4	35.561561897934496	37.0	35.0	37.0	33.0	37.0
5	31.831164030646534	37.0	35.0	37.0	0.0	37.0
6	33.7897027196559	37.0	35.0	37.0	17.0	37.0
7	20.983612617052735	32.0	0.0	37.0	0.0	37.0
8	28.14293875173619	35.0	17.0	37.0	17.0	37.0
9	34.6418634347417	35.0	32.0	39.0	28.0	39.0
10	36.169093597383394	37.0	35.0	39.0	32.0	39.0
11	36.62317980196245	38.0	35.0	39.0	33.0	39.0
12	36.95377257045566	39.0	37.0	39.0	33.0	39.0
13	36.67444217930911	39.0	35.0	39.0	33.0	39.0
14	38.06168511134011	40.0	37.0	41.0	33.0	41.0
15	38.19057193422644	40.0	37.0	41.0	33.0	41.0
16	38.28243872933375	40.0	37.0	41.0	34.0	41.0
17	38.22428760249115	40.0	37.0	41.0	34.0	41.0
18	38.11475648550562	40.0	37.0	41.0	33.0	41.0
19	38.033250817688966	40.0	37.0	41.0	34.0	41.0
20	37.98042587033469	40.0	36.0	41.0	34.0	41.0
21	37.911560777812625	40.0	36.0	41.0	33.0	41.0
22	37.86291388503069	39.0	36.0	41.0	33.0	41.0
23	37.811153053452216	39.0	36.0	41.0	33.0	41.0
24	37.63070142031453	39.0	35.0	41.0	33.0	41.0
25	37.50122653344684	39.0	35.0	41.0	33.0	41.0
26	37.333986737757066	39.0	35.0	41.0	33.0	41.0
27	37.29542094179847	39.0	35.0	41.0	33.0	41.0
28	37.19936489090013	39.0	35.0	41.0	33.0	41.0
29	37.15323267171468	39.0	35.0	41.0	32.0	41.0
30	36.896847977059906	39.0	35.0	41.0	31.0	41.0
31	36.77430664456293	39.0	35.0	40.0	31.0	41.0
32	36.614487656257	39.0	35.0	40.0	31.0	41.0
33	36.31348290694027	39.0	35.0	40.0	30.0	41.0
34	36.110645638245444	39.0	35.0	40.0	30.0	41.0
35	35.79562256373494	39.0	35.0	40.0	27.0	41.0
36	35.52989605269053	38.0	35.0	40.0	26.0	41.0
37	35.47317868184059	38.0	35.0	40.0	25.0	41.0
38	35.265575294592054	38.0	34.0	40.0	24.0	41.0
39	35.160743088848065	38.0	34.0	40.0	24.0	41.0
40	35.01809556879788	38.0	34.0	40.0	23.0	41.0
41	34.84717057215825	38.0	34.0	40.0	23.0	41.0
42	34.77604283346028	38.0	34.0	40.0	23.0	41.0
43	34.600094090237015	38.0	34.0	40.0	22.0	41.0
44	34.45698171961109	38.0	33.0	40.0	22.0	41.0
45	34.361244903445495	38.0	33.0	40.0	22.0	41.0
46	34.13887830996013	38.0	33.0	40.0	20.0	41.0
47	34.08524127425064	38.0	33.0	40.0	20.0	41.0
48	33.99078699762534	37.0	33.0	40.0	20.0	41.0
49	33.84326134683454	37.0	33.0	40.0	20.0	41.0
50	33.7021315919172	37.0	33.0	40.0	19.0	41.0
51	31.70452305210807	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	8.0
11	6.0
12	7.0
13	1.0
14	4.0
15	9.0
16	24.0
17	27.0
18	55.0
19	127.0
20	252.0
21	419.0
22	651.0
23	1130.0
24	1708.0
25	2521.0
26	3101.0
27	3516.0
28	3648.0
29	3893.0
30	4448.0
31	5332.0
32	7173.0
33	9925.0
34	13134.0
35	16688.0
36	19336.0
37	30406.0
38	31271.0
39	19715.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.013531072180655	18.86789282673955	23.538801021551144	15.579775079528652
2	33.76887405349702	26.67122182893499	23.497916573323177	16.06198754424481
3	30.89632151978135	24.898628970832025	28.69416192481742	15.510887584569202
4	27.200479412160046	24.2170348133877	30.835834938841344	17.746650835610914
5	22.446122138088622	36.60838299206954	24.3189659034903	16.62652896635154
6	24.24559792105381	35.45969801514405	27.898315336708635	12.396388727093507
7	44.634056185312964	42.75617187149962	9.270688650925221	3.339083292262198
8	74.02941440028675	8.53588870469107	13.329450244186566	4.105246650835611
9	67.8989874098302	9.201241095031138	15.308705587167884	7.591065907970787
10	38.39161700793046	25.73087951969174	22.334670012097316	13.542833460280479
11	30.749585554908375	24.719969532685155	27.99184551279179	16.53859939961468
12	29.015076840360233	22.875128814014964	31.485505623011782	16.62428872261302
13	22.94065594336664	26.122362112997894	33.011671669877686	17.925310273757784
14	18.251265737712263	28.705923204444645	32.028204668667954	21.014606389175142
15	18.23950445808504	25.272749675164658	38.007415206774496	18.480330659975806
16	20.868990546171425	24.51834759621847	34.84083068237824	19.771831175231867
17	19.99249518347596	24.796137819794794	32.532259509834674	22.679107486894573
18	21.278955150320357	25.883776154845645	32.999910390250456	19.83735830458354
19	23.266051346386487	26.390071239750885	29.60762130919844	20.73625610466419
20	24.21479456964918	25.495093866212642	32.28079215018594	18.009319413952237
21	23.04874770375017	27.81542631838344	30.54012276535687	18.59570321250952
22	21.488978000806487	24.78885702764461	30.979210538106543	22.742954433442357
23	21.152941440028673	26.75803127380259	32.043886374837584	20.045140911331153
24	20.938998163000132	24.399054617142347	33.398113714772165	21.26383350508535
25	19.884403423092433	27.204959899637082	31.53031049778216	21.38032617948833
26	19.101438236480128	26.89804650746001	32.31551592813298	21.684999327926878
27	20.27868632107173	27.600362919485637	31.61207939423809	20.508871365204534
28	17.353488059500872	26.89860656839464	34.47847125767283	21.26943411443165
29	21.93870693131413	25.057686276266857	32.07805009184999	20.92555670056902
30	20.026098839553743	27.17751691384023	33.02175276670102	19.774631479905015
31	23.215085801335185	25.55726062995654	29.219499081500068	22.00815448720821
32	22.970339172902012	27.294009588243203	28.798893319593173	20.936757919261613
33	21.10925668712756	27.12543124691966	29.432322236659346	22.332989829293425
34	20.12410950311394	27.45922756395896	30.67957793807966	21.73708499484744
35	20.136430843675793	26.14868497692549	31.08674223755545	22.62814194184327
36	20.71049330167122	28.85881983959855	30.00750481652404	20.423182042206193
37	20.15379273264931	28.327882073569604	30.669496841256326	20.848828352524755
38	19.551727227922395	29.231260361127294	28.474057977507954	22.742954433442357
39	21.065571934226444	27.945920516152157	29.311349074779336	21.677158474842063
40	21.32768045163314	26.56425019042072	29.813163672207537	22.29490568573861
41	18.442806577355615	28.137461355795512	29.98902280568126	23.430709261167614
42	19.300259868273667	26.075316994489	30.868318473049865	23.756104664187465
43	20.614722881849545	25.777924638200638	30.119517003449975	23.487835476499843
44	19.788072942336125	27.00333796317039	29.584658810878622	23.623930283614857
45	20.246762847797843	27.606523589766567	28.411331152829426	23.735382409606164
46	20.902034141314573	29.087884761862092	28.110018369998656	21.90006272682468
47	19.72198575204982	26.70370536314351	31.558313544513645	22.015995340293024
48	19.558447959137954	25.994108158967695	31.08338187194767	23.364062009946682
49	19.209529996863658	25.67823379183655	32.22366593485371	22.888570276446078
50	19.222411398360144	26.154845647206415	30.84759621846857	23.77514673596487
51	19.414512298938124	25.174178950669834	29.469846319279537	25.941462431112505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	434.0
2	631.0
3	2640.5
4	4650.0
5	3121.0
6	1592.0
7	1371.0
8	1150.0
9	1081.5
10	1013.0
11	1003.0
12	993.0
13	969.0
14	945.0
15	940.0
16	935.0
17	921.5
18	908.0
19	904.5
20	901.0
21	1018.0
22	1135.0
23	1246.5
24	1358.0
25	1727.0
26	2291.0
27	2486.0
28	2689.0
29	2892.0
30	3440.5
31	3989.0
32	4529.0
33	5069.0
34	5462.5
35	5856.0
36	6021.0
37	6186.0
38	6818.0
39	7450.0
40	8210.5
41	8971.0
42	9853.5
43	10736.0
44	11551.0
45	12366.0
46	13815.5
47	15265.0
48	14352.0
49	13439.0
50	12854.0
51	12269.0
52	11106.5
53	9944.0
54	9276.5
55	8609.0
56	8066.0
57	7523.0
58	7276.5
59	7030.0
60	6702.5
61	6375.0
62	5526.0
63	4677.0
64	3882.5
65	3088.0
66	2519.5
67	1951.0
68	1652.5
69	1354.0
70	1162.0
71	970.0
72	759.0
73	548.0
74	477.5
75	319.5
76	232.0
77	178.5
78	125.0
79	114.5
80	104.0
81	75.5
82	47.0
83	36.0
84	25.0
85	17.0
86	9.0
87	7.5
88	6.0
89	6.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	178552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37412070433264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95517210708886	39.269232492495185
2	7.916036645023571	7.9752677091267525
3	3.5733345192564263	5.400107531699449
4	2.2691897180467846	4.5723374703167705
5	1.6932758160633283	4.264864017205072
6	1.2607844881259451	3.810654599220395
7	1.0706661922974294	3.7753707603387245
8	0.8182869340923241	3.2976387830996012
9	0.6315040469625545	2.8630314978269635
>10	2.75949479676243	20.11794883283301
>50	0.030018678288712977	1.029391997849366
>100	0.020012452192475317	2.2161611183296746
>500	0.0011118028995819621	0.523656973878758
>1k	0.0011118028995819621	0.884336215780277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1579	0.884336215780277	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.523656973878758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	405	0.22682467852502353	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.2189838254402079	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	348	0.19490120525113133	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	323	0.18089968188538913	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCG	271	0.1517765132846454	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	257	0.14393566019982976	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGC	236	0.1321743805726063	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	230	0.12881401496482817	No Hit
GAATCTATCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTC	220	0.1232134056185313	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044804874770375015	0.0
2	0.0	0.0	0.0	0.33827680451633135	0.0
3	0.0	0.0	0.0	0.4194856400376361	0.0
4	0.0	0.0	0.0	0.5634213002374658	0.0
5	0.0	0.0	0.0	1.1794883283301223	0.0
6	0.0	0.0	0.0	1.4091133115282943	0.0
7	0.0	0.0	0.0	1.7546709081948115	0.0
8	0.0	0.0	0.0	2.444665979658587	0.0
9	0.0	0.0	0.0	2.6748510237913883	0.0
10	0.0	0.0	0.0	3.8879430081992923	0.0
11	0.0	0.0	0.0	4.640664904341592	0.0
12	5.600609346296877E-4	0.0	0.0	5.732223665934853	0.0
13	5.600609346296877E-4	0.0	0.0	5.986491330256732	0.0
14	5.600609346296877E-4	0.0	0.0	6.123706259241005	0.0
15	5.600609346296877E-4	0.0	0.0	6.392535507863255	0.0
16	5.600609346296877E-4	0.0	0.0	6.8876293740759	0.0
17	5.600609346296877E-4	0.0	0.0	7.4359290290783635	0.0
18	5.600609346296877E-4	0.0	0.0	8.020632644831757	0.0
19	5.600609346296877E-4	0.0	0.0	8.348828352524755	0.0
20	5.600609346296877E-4	0.0	0.0	8.687665217975717	0.0
21	5.600609346296877E-4	0.0	0.0	9.146915184372059	0.0
22	5.600609346296877E-4	0.0	0.0	9.566400824409696	0.0
23	5.600609346296877E-4	0.0	0.0	9.995967561270666	0.0
24	5.600609346296877E-4	0.0	0.0	10.365607778126261	0.0
25	5.600609346296877E-4	0.0	0.0	10.681482145257403	0.0
26	5.600609346296877E-4	0.0	0.0	10.9587123078991	0.0
27	5.600609346296877E-4	0.0	0.0	11.277947040638022	0.0
28	5.600609346296877E-4	0.0	0.0	11.596061651507684	0.0
29	5.600609346296877E-4	0.0	0.0	11.916976567050495	0.0
30	5.600609346296877E-4	0.0	0.0	12.30229849007572	0.0
31	5.600609346296877E-4	0.0	0.0	12.670258524127425	0.0
32	5.600609346296877E-4	0.0	0.0	12.994533805278014	0.0
33	5.600609346296877E-4	0.0	0.0	13.370894753349164	0.0
34	5.600609346296877E-4	0.0	0.0	13.73773466553161	0.0
35	5.600609346296877E-4	0.0	0.0	14.084972445002016	0.0
36	5.600609346296877E-4	0.0	0.0	14.453492539988352	0.0
37	5.600609346296877E-4	0.0	0.0	14.791769344504683	0.0
38	5.600609346296877E-4	0.0	0.0	15.156929073883239	0.0
39	5.600609346296877E-4	0.0	0.0	15.53104977821587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGAG	25	3.8775353E-5	45.000004	2
CACATCC	25	3.8775353E-5	45.000004	45
TGAAACG	30	2.1551768E-6	45.000004	4
TAGCATA	25	3.8775353E-5	45.000004	30
AAACGGC	30	2.1551768E-6	45.000004	6
TCGTCCC	30	2.1551768E-6	45.000004	38
ATCGGGG	30	2.1551768E-6	45.000004	4
CAATAAG	25	3.8775353E-5	45.000004	2
ACAAGGC	25	3.8775353E-5	45.000004	4
ACGACGG	25	3.8775353E-5	45.000004	2
AGGACCG	35	1.2042256E-7	45.0	7
AACGGCT	20	7.0172723E-4	45.0	7
AGGCCTA	20	7.0172723E-4	45.0	1
CAAGGCG	20	7.0172723E-4	45.0	5
GCGATGT	55	1.8189894E-12	45.0	9
AACCTGC	20	7.0172723E-4	45.0	33
CAATCAT	20	7.0172723E-4	45.0	12
TAGTATC	20	7.0172723E-4	45.0	38
CGTTTTA	275	0.0	44.18182	1
CTATCTC	90	0.0	42.5	5
>>END_MODULE