SRR3551107_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241624
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2643	1.0938482932159057	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	1.0777075124987583	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	1885	0.7801377346621197	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	1818	0.7524087011224051	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	1610	0.6663245372976194	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.5926563586398702	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	1256	0.5198159123265901	No Hit
GAATCTATCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	1063	0.4399397410853227	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	665	0.27522100453597326	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTT	626	0.25908022381882595	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT	551	0.22804026090123497	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.2255570638678277	No Hit
GAATCTATCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTT	497	0.20569148760056946	No Hit
GAATATGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	494	0.20444988908386585	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	452	0.1870675098500149	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	449	0.18582591133331125	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTC	423	0.17506539085521305	No Hit
GAATGAATGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT	402	0.16637420123828758	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT	357	0.147750223487733	No Hit
GCTGTCACTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	283	0.11712412674237659	TruSeq Adapter, Index 21 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	278	0.11505479588120385	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTC	265	0.10967453564215475	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	255	0.10553587391980929	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	95	0.0	45.000004	7
AACGGCT	120	0.0	45.000004	7
CTACGAA	30	2.158362E-6	45.000004	11
TACGAAT	30	2.158362E-6	45.000004	12
CGAATAT	30	2.158362E-6	45.000004	14
CTTCGAT	20	7.022218E-4	45.0	32
ATAGGCG	40	6.7757355E-9	45.0	5
AACCCCG	20	7.022218E-4	45.0	37
GCGCGAC	25	3.8816324E-5	45.0	9
CGACAGG	20	7.022218E-4	45.0	3
ACCTATC	20	7.022218E-4	45.0	3
ACGGCTC	20	7.022218E-4	45.0	33
GTCGTTT	25	3.8816324E-5	45.0	9
TAAGGTG	20	7.022218E-4	45.0	5
ACATAGT	45	3.8198777E-10	45.0	35
AGGGCGC	20	7.022218E-4	45.0	7
CCACGGT	20	7.022218E-4	45.0	43
CCTGCGT	20	7.022218E-4	45.0	22
TGCGTTC	20	7.022218E-4	45.0	24
ACGGTGT	20	7.022218E-4	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content