##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241624
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720702413667517	31.0	31.0	34.0	30.0	34.0
2	31.84886848988511	31.0	31.0	34.0	30.0	34.0
3	31.53130483726782	31.0	31.0	34.0	30.0	34.0
4	35.469158692845085	37.0	35.0	37.0	33.0	37.0
5	31.80769708307122	37.0	35.0	37.0	0.0	37.0
6	33.71885657053935	37.0	35.0	37.0	19.0	37.0
7	20.866255007780683	32.0	0.0	37.0	0.0	37.0
8	27.91150713505281	35.0	17.0	37.0	17.0	37.0
9	34.37495447472105	35.0	32.0	39.0	28.0	39.0
10	36.052490646624506	37.0	35.0	39.0	32.0	39.0
11	36.49755405092209	37.0	35.0	39.0	32.0	39.0
12	36.83648147535013	39.0	35.0	39.0	33.0	39.0
13	36.55639340462868	39.0	35.0	39.0	32.0	39.0
14	38.065068039598714	40.0	37.0	41.0	33.0	41.0
15	38.07485597457206	40.0	37.0	41.0	33.0	41.0
16	38.21731284971692	40.0	37.0	41.0	34.0	41.0
17	38.045036916862564	40.0	37.0	41.0	33.0	41.0
18	37.871287620435055	40.0	37.0	41.0	33.0	41.0
19	37.769596563255305	40.0	37.0	41.0	33.0	41.0
20	37.77271297553223	39.0	36.0	41.0	33.0	41.0
21	37.78569181869351	39.0	36.0	41.0	33.0	41.0
22	37.73714945535212	39.0	36.0	41.0	33.0	41.0
23	37.74895705724597	39.0	36.0	41.0	33.0	41.0
24	37.53586978114757	39.0	35.0	41.0	33.0	41.0
25	37.47979505347151	39.0	35.0	41.0	33.0	41.0
26	37.24660629738768	39.0	35.0	40.0	33.0	41.0
27	37.303276992351755	39.0	35.0	40.0	33.0	41.0
28	37.20314621064133	39.0	35.0	40.0	33.0	41.0
29	37.1447993576797	39.0	35.0	40.0	32.0	41.0
30	37.04866238453134	39.0	35.0	40.0	32.0	41.0
31	36.904641095255435	39.0	35.0	40.0	32.0	41.0
32	36.68454706486111	39.0	35.0	40.0	31.0	41.0
33	36.502334205211405	39.0	35.0	40.0	30.0	41.0
34	36.170616329503694	39.0	35.0	40.0	30.0	41.0
35	35.617020660199316	39.0	35.0	40.0	25.0	41.0
36	35.56199715260073	38.0	35.0	40.0	25.0	41.0
37	35.502644604840576	38.0	35.0	40.0	25.0	41.0
38	35.07735986491408	38.0	34.0	40.0	24.0	41.0
39	34.99154885276297	38.0	34.0	40.0	23.0	41.0
40	34.40565506737741	38.0	33.0	40.0	21.0	41.0
41	34.32029516935404	38.0	33.0	40.0	21.0	41.0
42	34.207884978313416	38.0	33.0	40.0	20.0	41.0
43	33.94140896599676	38.0	33.0	40.0	18.0	41.0
44	33.821189120286064	38.0	33.0	40.0	17.0	41.0
45	33.757979339800684	37.0	33.0	40.0	18.0	41.0
46	33.303955732874215	37.0	32.0	40.0	15.0	41.0
47	33.25797520113896	37.0	32.0	40.0	15.0	41.0
48	32.917913783399	37.0	31.0	40.0	13.0	41.0
49	32.78311757110221	37.0	31.0	40.0	13.0	41.0
50	32.7521479654339	37.0	31.0	40.0	13.0	41.0
51	30.35313545012085	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	9.0
11	5.0
12	7.0
13	2.0
14	5.0
15	12.0
16	22.0
17	53.0
18	120.0
19	197.0
20	335.0
21	529.0
22	938.0
23	1587.0
24	2520.0
25	3682.0
26	4775.0
27	5467.0
28	5833.0
29	6302.0
30	7170.0
31	8476.0
32	10166.0
33	13655.0
34	17671.0
35	22061.0
36	25667.0
37	39796.0
38	39772.0
39	24779.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.325282256729466	17.16013309936099	20.287305896765222	16.227278747144325
2	31.33173856901632	32.30597953845645	21.240853557593617	15.121428334933615
3	26.597109558653116	32.123050690328775	28.32913948945469	12.950700261563423
4	25.257010892957656	21.50407244313479	37.544283680429096	15.694632983478463
5	24.898602787802538	33.36754627023806	24.288977916101047	17.44487302585836
6	22.03589047445618	37.62622918253153	28.980150978379633	11.357729364632654
7	41.10229116312949	42.506539085521304	13.253236433466874	3.1379333178823297
8	67.37079098102838	11.979770221501175	15.945435883852596	4.7040029136178525
9	61.916034830977054	8.509502367314505	15.582061384630666	13.992401417077774
10	36.470714829652685	24.28732245141211	24.453282786478166	14.788679932457041
11	28.21615402443466	23.18271363771811	29.881137635334238	18.719994702512995
12	27.07181405820614	20.559629838095553	33.91302188524319	18.45553421845512
13	20.130036751316094	23.825447803198358	38.7627056914876	17.281809753997948
14	17.639390126808596	28.32996722179916	32.04276065291528	21.987881998476972
15	16.10146343078502	24.59523888355461	42.47715458729265	16.826143098367712
16	18.301576002383868	25.167201933582756	32.880425785518	23.65079627851538
17	18.24156540740986	23.49973512564977	35.020941628315065	23.237757838625303
18	18.640946263616197	26.942273946296723	34.27474091977618	20.1420388703109
19	20.28896136145416	27.046154355527598	30.016471873654936	22.64841240936331
20	21.54173426480813	25.93947621097242	35.29367943581763	17.225110088401816
21	20.540591994172765	29.198672317319474	31.85652087540973	18.404214813098037
22	18.943482435519652	23.94960765486872	31.391749163990333	25.715160745621297
23	19.289888421679965	28.49013343045393	33.11591563751945	19.104062510346655
24	21.469307684667086	23.40702910306923	31.764228719001427	23.35943449326226
25	18.718753103996292	30.966708605105453	30.185743138098864	20.12879515279939
26	18.081813064927324	25.10512200774758	32.98430619474886	23.828758732576233
27	20.95901069430189	26.67946892692779	30.535460053637053	21.826060325133266
28	16.158163096381152	24.819968215077974	36.15617653875443	22.865692149786447
29	21.227195973909875	22.889282521603814	33.82942091845181	22.0541005860345
30	18.48657418137271	26.568138926596696	35.211320067542964	19.733966824487634
31	22.564397576399696	23.264245273648314	29.54052577558521	24.630831374366785
32	22.805267688640203	25.601347548256797	30.603334105883523	20.99005065721948
33	22.342979174254214	23.400821110485715	29.617091017448598	24.639108697811476
34	22.998543191073733	23.09456014303215	32.823726119921865	21.083170545972255
35	20.022431546535113	22.438582260040395	35.75969274575373	21.77929344767076
36	21.863308280634374	24.441694533655596	33.34064496904281	20.35435221666722
37	18.802767936959906	24.7268483263252	34.78338244545244	21.687001291262455
38	21.627818428632917	25.711435950071188	28.91889878488892	23.741846836406978
39	25.25949408999106	23.478214084693576	30.1406317253253	21.121660099990066
40	22.469622222957987	23.328808396516905	35.13723802271297	19.064331357812137
41	20.048091249213655	25.688673310598286	33.01369069297752	21.249544747210543
42	18.52547760156276	23.77371453166904	37.046816541403174	20.653991325365027
43	22.293315233586068	22.900870774426384	31.806443068569344	22.9993709234182
44	21.266927126444394	24.061765387544284	31.026305333907228	23.645002152104095
45	18.94803496341423	24.34940237724729	30.99899016653975	25.703572492798727
46	23.488146872827205	27.063536734761446	28.63415885839155	20.8141575340198
47	18.94803496341423	22.767192000794623	37.69162003774459	20.593152998046552
48	20.906449690428104	24.929228884547893	31.093351653809226	23.07096977121478
49	18.497748568023045	23.480697281726982	35.606562262026955	22.414991888223025
50	19.298993477469125	23.201337615468663	33.62993742343476	23.869731483627454
51	20.164387643611562	22.72870244677681	30.552842432870907	26.554067476740723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	587.0
1	724.0
2	861.0
3	4072.5
4	7284.0
5	4826.5
6	2369.0
7	1954.5
8	1540.0
9	1454.0
10	1368.0
11	1300.0
12	1232.0
13	1186.0
14	1140.0
15	1096.0
16	1052.0
17	1049.5
18	1047.0
19	1096.0
20	1145.0
21	1273.5
22	1402.0
23	1554.0
24	1706.0
25	1944.0
26	2560.0
27	2938.0
28	3499.0
29	4060.0
30	4759.5
31	5459.0
32	5985.0
33	6511.0
34	6826.0
35	7141.0
36	7545.0
37	7949.0
38	8666.0
39	9383.0
40	10612.5
41	11842.0
42	13111.5
43	14381.0
44	17939.0
45	21497.0
46	25892.0
47	30287.0
48	24049.5
49	17812.0
50	16404.5
51	14997.0
52	13315.0
53	11633.0
54	10766.0
55	9899.0
56	9487.0
57	9075.0
58	8638.0
59	8201.0
60	7684.0
61	7167.0
62	6447.5
63	5728.0
64	4795.0
65	3862.0
66	3154.0
67	2446.0
68	2019.0
69	1592.0
70	1384.5
71	1177.0
72	950.0
73	723.0
74	593.5
75	346.0
76	228.0
77	180.0
78	132.0
79	92.0
80	52.0
81	47.0
82	42.0
83	28.0
84	14.0
85	9.0
86	4.0
87	4.0
88	4.0
89	4.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.93238997518466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41264250216997	33.13057619464517
2	8.821489596661	7.750982422450911
3	3.9652862295670404	5.2261350300170655
4	2.5170791432633512	4.423252100809971
5	1.8224613816314634	4.003254206627365
6	1.4830375689714803	3.9092030896742944
7	1.1237461328179617	3.455819733804505
8	0.961071268450832	3.377772620762188
9	0.7325297921720658	2.8963606048329757
>10	3.0558667031936895	19.276186853136494
>50	0.05389183599488087	1.6717866580670961
>100	0.038921881551858405	3.5575339479560495
>500	0.003991987851472657	1.0465656314728975
>1k	0.007983975702945314	6.274570905743039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2643	1.0938482932159057	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	1.0777075124987583	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	1885	0.7801377346621197	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	1818	0.7524087011224051	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	1610	0.6663245372976194	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	0.5926563586398702	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	1256	0.5198159123265901	No Hit
GAATCTATCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	1063	0.4399397410853227	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	665	0.27522100453597326	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTT	626	0.25908022381882595	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT	551	0.22804026090123497	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.2255570638678277	No Hit
GAATCTATCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTT	497	0.20569148760056946	No Hit
GAATATGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	494	0.20444988908386585	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	452	0.1870675098500149	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	449	0.18582591133331125	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTC	423	0.17506539085521305	No Hit
GAATGAATGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT	402	0.16637420123828758	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCT	357	0.147750223487733	No Hit
GCTGTCACTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	283	0.11712412674237659	TruSeq Adapter, Index 21 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	278	0.11505479588120385	No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTC	265	0.10967453564215475	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	255	0.10553587391980929	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.138661722345462E-4	0.0	0.0	0.09229215640830381	0.0
2	4.138661722345462E-4	0.0	0.0	0.6650829387809158	0.0
3	4.138661722345462E-4	0.0	0.0	0.7805516008343542	0.0
4	4.138661722345462E-4	0.0	0.0	1.164619408668013	0.0
5	4.138661722345462E-4	0.0	0.0	3.0270171837234714	0.0
6	4.138661722345462E-4	0.0	0.0	3.37466476840049	0.0
7	4.138661722345462E-4	0.0	0.0	4.103483097705526	0.0
8	4.138661722345462E-4	0.0	0.0	5.021852133893984	0.0
9	4.138661722345462E-4	0.0	0.0	5.22630202297785	0.0
10	4.138661722345462E-4	0.0	0.0	8.749130881038308	0.0
11	4.138661722345462E-4	0.0	0.0	9.331440585372315	0.0
12	4.138661722345462E-4	0.0	0.0	11.821259477535344	0.0
13	4.138661722345462E-4	0.0	0.0	12.15773267556203	0.0
14	4.138661722345462E-4	0.0	0.0	12.341903122206404	0.0
15	4.138661722345462E-4	0.0	0.0	12.770668476641394	0.0
16	4.138661722345462E-4	0.0	0.0	13.208952753037778	0.0
17	4.138661722345462E-4	0.0	0.0	13.651375691156508	0.0
18	4.138661722345462E-4	0.0	0.0	14.10124822037546	0.0
19	4.138661722345462E-4	0.0	0.0	14.402956659934443	0.0
20	4.138661722345462E-4	0.0	0.0	14.696801642220972	0.0
21	4.138661722345462E-4	0.0	0.0	15.028722312353077	0.0
22	4.138661722345462E-4	0.0	0.0	15.407823726119922	0.0
23	4.138661722345462E-4	0.0	0.0	15.745952388835546	0.0
24	4.138661722345462E-4	0.0	0.0	16.063387742939444	0.0
25	4.138661722345462E-4	0.0	0.0	16.330331424030724	0.0
26	4.138661722345462E-4	0.0	0.0	16.57492633182134	0.0
27	4.138661722345462E-4	0.0	0.0	16.81869350726749	0.0
28	4.138661722345462E-4	0.0	0.0	17.07984306194749	0.0
29	4.138661722345462E-4	0.0	0.0	17.356305665000164	0.0
30	4.138661722345462E-4	0.0	0.0	17.65884183690362	0.0
31	4.138661722345462E-4	0.0	0.0	17.957239347084727	0.0
32	4.138661722345462E-4	0.0	0.0	18.251912061715725	0.0
33	4.138661722345462E-4	0.0	0.0	18.513889348740193	0.0
34	4.138661722345462E-4	0.0	0.0	18.82511671026057	0.0
35	4.138661722345462E-4	0.0	0.0	19.13924113498659	0.0
36	4.138661722345462E-4	0.0	0.0	19.41528987186703	0.0
37	4.138661722345462E-4	0.0	0.0	19.714101248220377	0.0
38	4.138661722345462E-4	0.0	0.0	20.00297983644009	0.0
39	8.277323444690924E-4	0.0	0.0	20.31172400092706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	95	0.0	45.000004	7
AACGGCT	120	0.0	45.000004	7
CTACGAA	30	2.158362E-6	45.000004	11
TACGAAT	30	2.158362E-6	45.000004	12
CGAATAT	30	2.158362E-6	45.000004	14
CTTCGAT	20	7.022218E-4	45.0	32
ATAGGCG	40	6.7757355E-9	45.0	5
AACCCCG	20	7.022218E-4	45.0	37
GCGCGAC	25	3.8816324E-5	45.0	9
CGACAGG	20	7.022218E-4	45.0	3
ACCTATC	20	7.022218E-4	45.0	3
ACGGCTC	20	7.022218E-4	45.0	33
GTCGTTT	25	3.8816324E-5	45.0	9
TAAGGTG	20	7.022218E-4	45.0	5
ACATAGT	45	3.8198777E-10	45.0	35
AGGGCGC	20	7.022218E-4	45.0	7
CCACGGT	20	7.022218E-4	45.0	43
CCTGCGT	20	7.022218E-4	45.0	22
TGCGTTC	20	7.022218E-4	45.0	24
ACGGTGT	20	7.022218E-4	45.0	45
>>END_MODULE