FastQCFastQC Report
Sat 18 Jun 2016
SRR3551106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672065
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68131.0137412303869417No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.5480124690320133No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC12630.18792825098762767No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG8800.13093971565250384No Hit
GAATCTATCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC8210.12216080289852917No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.10683490436192927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG302.1641517E-644.9999962
ACGGTTA253.8890714E-544.99999634
CGTTTTA15050.044.1029931
CGTTATT5800.043.836211
CGTTTTT27050.042.3382641
TGGGCGA2300.042.0652166
ATGGGCG1050.040.7142875
GACCGAT2900.038.7931069
CGAAGGA1800.038.754
ACCGATC356.244416E-638.5714310
GCCGATC356.244416E-638.571439
ACATACG1350.038.33333617
AGGCGAT3700.038.3108147
AACGAGC2000.038.24999615
TAGGCGA1300.038.076926
CCTTATC950.037.89473343
GCGATGT1250.037.89
CGGTTTA1550.037.741941
CGTATTG301.1394175E-437.49999613
CCGTAGA301.1394175E-437.4999961
Produced by FastQC (version 0.11.5)