##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124602
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564220478001957	31.0	31.0	34.0	30.0	34.0
2	31.734249851527263	31.0	31.0	34.0	30.0	34.0
3	31.21134492223239	31.0	31.0	34.0	28.0	34.0
4	35.25863148264073	37.0	35.0	37.0	33.0	37.0
5	31.732179258759892	35.0	35.0	37.0	0.0	37.0
6	33.6860403524823	37.0	35.0	37.0	18.0	37.0
7	21.04765573586299	32.0	0.0	37.0	0.0	37.0
8	28.16643392561917	35.0	17.0	37.0	17.0	37.0
9	34.54518386542752	35.0	32.0	39.0	28.0	39.0
10	36.047559429222645	37.0	35.0	39.0	32.0	39.0
11	36.56366671481999	38.0	35.0	39.0	32.0	39.0
12	36.97695863629797	39.0	37.0	39.0	33.0	39.0
13	36.75242772989198	39.0	37.0	39.0	33.0	39.0
14	38.12063209258278	40.0	37.0	41.0	33.0	41.0
15	38.281472207508706	40.0	37.0	41.0	34.0	41.0
16	38.3053642798671	40.0	38.0	41.0	34.0	41.0
17	38.269032599797754	40.0	37.0	41.0	34.0	41.0
18	38.11699651690984	40.0	37.0	41.0	33.0	41.0
19	37.99593104444551	40.0	37.0	41.0	34.0	41.0
20	37.8772652124364	39.0	36.0	41.0	34.0	41.0
21	37.76426542110079	39.0	36.0	41.0	33.0	41.0
22	37.67860869006918	39.0	36.0	41.0	33.0	41.0
23	37.60054413251794	39.0	35.0	41.0	33.0	41.0
24	37.410916357682865	39.0	35.0	41.0	33.0	41.0
25	37.260637870981206	39.0	35.0	40.0	33.0	41.0
26	37.12319224410523	39.0	35.0	40.0	32.0	41.0
27	37.065649026500374	39.0	35.0	40.0	32.0	41.0
28	36.947400523266076	39.0	35.0	40.0	32.0	41.0
29	36.94913404279225	39.0	35.0	40.0	32.0	41.0
30	36.739281873485176	39.0	35.0	40.0	31.0	41.0
31	36.55450153288069	39.0	35.0	40.0	31.0	41.0
32	36.42770581531597	39.0	35.0	40.0	31.0	41.0
33	36.10568851222292	38.0	35.0	40.0	30.0	41.0
34	35.72194667822346	38.0	35.0	40.0	27.0	41.0
35	35.415434744225614	38.0	35.0	40.0	25.0	41.0
36	35.051740742524196	38.0	34.0	40.0	24.0	41.0
37	34.91626940177525	38.0	34.0	40.0	23.0	41.0
38	34.75916116916262	38.0	34.0	40.0	22.0	41.0
39	34.65217251729507	38.0	34.0	40.0	22.0	41.0
40	34.48756841784241	38.0	34.0	40.0	21.0	41.0
41	34.351872361599334	38.0	33.0	40.0	20.0	41.0
42	34.21851174138457	38.0	33.0	40.0	20.0	41.0
43	34.052663681160816	38.0	33.0	40.0	18.0	41.0
44	33.83783566876936	38.0	33.0	40.0	18.0	41.0
45	33.68790228086227	38.0	33.0	40.0	18.0	41.0
46	33.44626892024205	37.0	32.0	40.0	15.0	41.0
47	33.37338084460924	37.0	32.0	40.0	15.0	41.0
48	33.254369913805554	37.0	32.0	40.0	15.0	41.0
49	33.10880242692733	37.0	32.0	40.0	15.0	41.0
50	32.91255357056869	36.0	31.0	40.0	14.0	41.0
51	30.865700390041894	35.0	27.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	2.0
11	3.0
12	3.0
13	3.0
14	2.0
15	7.0
16	11.0
17	21.0
18	46.0
19	82.0
20	177.0
21	284.0
22	557.0
23	900.0
24	1494.0
25	2173.0
26	2698.0
27	2888.0
28	2929.0
29	3068.0
30	3361.0
31	4080.0
32	5048.0
33	7055.0
34	9203.0
35	11653.0
36	13515.0
37	20436.0
38	20049.0
39	12845.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32598192645383	18.931477825396062	19.404182918412225	20.338357329737885
2	36.64628176112743	24.927368742074766	22.084717741288262	16.341631755509543
3	29.219434680021187	23.845524148890064	32.252291295484824	14.682749875603923
4	26.898444647758463	24.007640326800534	33.35821254875524	15.735702476685768
5	20.994847594741657	35.7434070079132	28.086226545320297	15.175518852024847
6	22.238808365836825	33.8397457504695	31.214587245790597	12.706858637903085
7	40.747339529060525	43.01134813245373	12.878605479847835	3.362706858637903
8	67.48206288823614	8.630680085391889	18.94672637678368	4.94053064958829
9	61.183608609813646	10.919567904206994	20.619251697404536	7.277571788574823
10	32.391935923981954	26.238744161409926	28.175310187637436	13.194009726970673
11	26.95542607662798	23.617598433411985	33.172822266095245	16.254153223864787
12	25.319818301471887	22.21392915041492	36.16555111474936	16.30070143336383
13	20.299032118264552	24.384841334810034	37.342097237604534	17.974029309320876
14	16.26378388789907	28.416076788494564	36.27550119580745	19.044638127798912
15	17.762154700566605	25.205052888396658	40.195181457761514	16.837610953275227
16	17.522190655045666	25.688993756119483	38.334055633135904	18.454759955698947
17	18.104845829119917	25.020465161072856	35.58530360668368	21.289385403123546
18	18.529397601964657	25.77245951108329	37.491372530135955	18.2067703568161
19	20.910579284441663	26.68335981765943	34.150334665575194	18.255726232323717
20	21.660165968443522	25.24116787852522	35.90391807515128	17.19474807787997
21	19.88170334344553	26.662493378918477	35.692846021733196	17.762957255902794
22	18.486862169146562	25.444214378581403	34.59495032182469	21.473973130447344
23	18.694724001219885	27.239530665639393	34.91677501163705	19.14897032150367
24	18.899375611948443	23.782924832667213	37.08688464069598	20.230814914688366
25	18.160222147317057	26.648047382867045	35.08611418757323	20.10561628224266
26	17.61929985072471	26.469880098232775	35.78915266207605	20.12166738896647
27	19.273366398613184	27.39683151153272	35.109388292322755	18.22041379753134
28	16.0936421566267	27.78607084958508	36.36538739346078	19.75489960032744
29	19.28139195197509	24.85915153849858	35.77149644467986	20.08796006484647
30	19.29985072470747	27.146434246641306	34.82688881398373	18.726826214667504
31	19.93547455097029	28.521211537535514	32.85340524229146	18.689908669202744
32	20.928235501837854	26.976292515368932	32.56047254458195	19.534999438211265
33	19.335163159499846	28.140800308181248	32.4224330267572	20.10160350556171
34	18.236464904255147	28.33180847819457	33.49625206657999	19.93547455097029
35	20.140928717035038	26.05495898942232	32.62708463748575	21.177027656056886
36	19.896149339496958	30.131137541933516	31.022776520441088	18.949936598128442
37	18.868878509173207	30.24510039967256	31.948122823068655	18.937898268085583
38	17.244506508723774	30.15200398067447	31.31330155214202	21.290187958459736
39	19.614452416494117	27.90484903934126	31.37911108970964	21.101587454454986
40	19.93547455097029	27.500361149901288	33.724980337394264	18.839183961734165
41	17.6618352835428	27.721063867353653	32.394343589990534	22.222757259113017
42	18.78220253286464	26.825412112165132	32.56368276592671	21.828702589043512
43	20.914592061122615	26.28208214956421	30.835781127108714	21.967544662204457
44	19.086371005280814	27.995537792330783	30.593409415579202	22.3246817868092
45	19.39294714370556	27.948187027495546	29.907224603136385	22.751641225662507
46	19.32312482945699	29.510762267058315	30.093417441132566	21.07269546235213
47	18.41944752090657	27.068586379030833	33.458531965779045	21.05343413428356
48	18.850419736440827	26.562173961894676	32.399961477343865	22.187444824320636
49	18.768559092149403	26.040512993370896	32.98582687276287	22.205101041716826
50	17.621707516733277	26.194603617919455	33.084541179114304	23.099147686232964
51	18.26294923034943	25.335869408195695	30.950546540183943	25.450634821270928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	284.5
2	409.0
3	3114.0
4	5819.0
5	3862.5
6	1906.0
7	1629.5
8	1353.0
9	1248.0
10	1143.0
11	1097.5
12	1052.0
13	1029.0
14	1006.0
15	939.5
16	873.0
17	867.5
18	862.0
19	890.5
20	919.0
21	1055.5
22	1192.0
23	1154.5
24	1117.0
25	1485.0
26	1935.0
27	2017.0
28	2161.0
29	2305.0
30	2691.5
31	3078.0
32	3447.5
33	3817.0
34	3930.0
35	4043.0
36	4261.5
37	4480.0
38	4776.5
39	5073.0
40	5489.5
41	5906.0
42	6464.0
43	7022.0
44	7797.0
45	8572.0
46	9635.0
47	10698.0
48	10323.0
49	9948.0
50	9326.0
51	8704.0
52	7456.5
53	6209.0
54	5509.0
55	4809.0
56	4507.5
57	4206.0
58	4096.5
59	3987.0
60	3709.5
61	3432.0
62	2975.5
63	2519.0
64	2049.5
65	1580.0
66	1314.0
67	1048.0
68	823.5
69	599.0
70	476.5
71	354.0
72	294.0
73	234.0
74	182.5
75	100.5
76	70.0
77	61.0
78	52.0
79	39.0
80	26.0
81	18.0
82	10.0
83	7.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	124602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.36501821800613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62106259294308	46.67340813149067
2	9.51737190753008	11.29997913356126
3	4.334189536298499	7.718977223479559
4	2.5983506827092064	6.170045424632028
5	1.627686900094633	4.831383123866391
6	0.9747194808706233	3.4718543843598013
7	0.6773016087603082	2.814561564019839
8	0.4366635122346897	2.0738029887160723
9	0.31499256455319724	1.6829585399913325
>10	0.854400432607814	7.538402272836713
>50	0.021630390698932	0.9004670872056628
>100	0.0189265918615655	2.1163384215341647
>500	0.0	0.0
>1k	0.0027037988373665	2.707821704306512
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2150	1.7254939728094252	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1224	0.9823277314970867	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.38281889536283525	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	283	0.2271231601418918	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	222	0.17816728463427553	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	218	0.17495706328951383	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	209	0.16773406526379994	RNA PCR Primer, Index 36 (95% over 22bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	191	0.1532880692123722	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	173	0.13884207316094443	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	153	0.12279096643713584	No Hit
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	135	0.10834497038570809	RNA PCR Primer, Index 15 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07624275693809088	0.0
2	0.0	0.0	0.0	0.42936710486188023	0.0
3	0.0	0.0	0.0	0.5569734033161586	0.0
4	0.0	0.0	0.0	0.7415611306399577	0.0
5	0.0	0.0	0.0	1.4373766071170606	0.0
6	0.0	0.0	0.0	1.7937111763856117	0.0
7	0.0	0.0	0.0	2.2784545994446317	0.0
8	0.0	0.0	0.0	3.3843758527150447	0.0
9	0.0	0.0	0.0	3.809730180895973	0.0
10	0.0	0.0	0.0	5.166851254393991	0.0
11	0.0	0.0	0.0	6.37790725670535	0.0
12	0.0	0.0	0.0	7.671626458644323	0.0
13	0.0	0.0	0.0	8.134700887626202	0.0
14	0.0	0.0	0.0	8.35620616041476	0.0
15	0.0	0.0	0.0	8.761496605190928	0.0
16	0.0	0.0	0.0	9.515898621209933	0.0
17	0.0	0.0	0.0	10.390683937657501	0.0
18	0.0	0.0	0.0	11.30399191024221	0.0
19	0.0	0.0	0.0	11.830468210783133	0.0
20	0.0	0.0	0.0	12.360154732668818	0.0
21	0.0	0.0	0.0	13.015039887000208	0.0
22	0.0	0.0	0.0	13.728511580873501	0.0
23	0.0	0.0	0.0	14.411486171971557	0.0
24	0.0	0.0	0.0	15.000561788735332	0.0
25	0.0	0.0	0.0	15.502961429190544	0.0
26	0.0	0.0	0.0	15.978074188215277	0.0
27	0.0	0.0	0.0	16.415466846439063	0.0
28	0.0	0.0	0.0	16.90101282483427	0.0
29	0.0	0.0	0.0	17.405820131298054	0.0
30	0.0	0.0	0.0	17.969213977303735	0.0
31	0.0	0.0	0.0	18.457970177043705	0.0
32	0.0	0.0	0.0	18.961974928171298	0.0
33	0.0	0.0	0.0	19.441903019213175	0.0
34	0.0	0.0	0.0	19.910595335548386	0.0
35	0.0	0.0	0.0	20.43386141474455	0.0
36	0.0	0.0	0.0	20.930643167846423	0.0
37	0.0	0.0	0.0	21.431437697629253	0.0
38	0.0	0.0	0.0	21.91778623136065	0.0
39	0.0	0.0	0.0	22.424198648496812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	75	0.0	45.000004	7
TTGCGGG	30	2.1498981E-6	45.000004	3
CATATGC	20	7.00907E-4	45.0	33
CGGTTTA	25	3.870747E-5	45.0	1
TTGTCTC	20	7.00907E-4	45.0	40
ACCTGGT	25	3.870747E-5	45.0	11
ACACAAC	25	3.870747E-5	45.0	11
GTGATTC	20	7.00907E-4	45.0	20
CACTGGT	20	7.00907E-4	45.0	24
TACGGGA	35	1.2002965E-7	45.0	4
TGACGGG	20	7.00907E-4	45.0	3
ACGGGAC	25	3.870747E-5	45.0	5
CGTTATT	155	0.0	45.0	1
AGGCCAC	20	7.00907E-4	45.0	7
TCATGCA	25	3.870747E-5	45.0	24
CTCATGC	20	7.00907E-4	45.0	23
AACGAGC	25	3.870747E-5	45.0	15
AACGAAG	20	7.00907E-4	45.0	2
GCCTAAA	20	7.00907E-4	45.0	9
CGAGATA	40	6.73208E-9	45.0	19
>>END_MODULE