##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316994
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93968024631381	31.0	31.0	34.0	30.0	34.0
2	32.12542193227632	33.0	31.0	34.0	30.0	34.0
3	31.90478368675748	33.0	31.0	34.0	30.0	34.0
4	35.736856218098765	37.0	35.0	37.0	35.0	37.0
5	32.10499567815164	37.0	35.0	37.0	10.0	37.0
6	33.950926515959296	37.0	35.0	37.0	19.0	37.0
7	20.798636567253638	32.0	0.0	37.0	0.0	37.0
8	28.063764613841272	35.0	17.0	37.0	17.0	37.0
9	34.70838880231172	35.0	32.0	39.0	30.0	39.0
10	36.25869574818451	37.0	35.0	39.0	32.0	39.0
11	36.69103200691496	38.0	35.0	39.0	33.0	39.0
12	37.040584364372826	39.0	37.0	39.0	34.0	39.0
13	36.80250099370966	39.0	37.0	39.0	33.0	39.0
14	38.2250358050941	40.0	38.0	41.0	34.0	41.0
15	38.368849252667246	40.0	38.0	41.0	34.0	41.0
16	38.477781913853256	40.0	38.0	41.0	34.0	41.0
17	38.407067641658834	40.0	38.0	41.0	34.0	41.0
18	38.302762828318514	40.0	37.0	41.0	34.0	41.0
19	38.219606680252625	40.0	37.0	41.0	34.0	41.0
20	38.18294983501265	40.0	37.0	41.0	34.0	41.0
21	38.120604806400124	40.0	37.0	41.0	34.0	41.0
22	38.08326656025035	40.0	37.0	41.0	34.0	41.0
23	38.0160192306479	40.0	36.0	41.0	34.0	41.0
24	37.86474191940542	40.0	36.0	41.0	33.0	41.0
25	37.73393818179524	40.0	36.0	41.0	33.0	41.0
26	37.551095604333206	40.0	35.0	41.0	33.0	41.0
27	37.49264023924743	39.0	35.0	41.0	33.0	41.0
28	37.395755124702674	39.0	35.0	41.0	33.0	41.0
29	37.33958055988442	40.0	35.0	41.0	33.0	41.0
30	37.18233783604737	39.0	35.0	41.0	32.0	41.0
31	37.048407856300116	39.0	35.0	41.0	32.0	41.0
32	36.87870117415471	39.0	35.0	41.0	31.0	41.0
33	36.71274535164704	39.0	35.0	41.0	31.0	41.0
34	36.52407301084563	39.0	35.0	41.0	30.0	41.0
35	36.305488431957706	39.0	35.0	41.0	30.0	41.0
36	36.165460544994545	39.0	35.0	41.0	29.0	41.0
37	36.02626232673174	39.0	35.0	41.0	29.0	41.0
38	35.933882029312855	39.0	35.0	41.0	28.0	41.0
39	35.87113636220244	39.0	35.0	41.0	27.0	41.0
40	35.73627261083806	39.0	35.0	40.0	27.0	41.0
41	35.633668145138394	39.0	35.0	40.0	26.0	41.0
42	35.54966655520294	39.0	35.0	40.0	26.0	41.0
43	35.447245058266084	38.0	35.0	40.0	26.0	41.0
44	35.306542079660815	38.0	34.0	40.0	25.0	41.0
45	35.25056310214073	38.0	34.0	40.0	24.0	41.0
46	35.12003066304094	38.0	34.0	40.0	24.0	41.0
47	35.0443162962075	38.0	34.0	40.0	24.0	41.0
48	34.92439289071717	38.0	34.0	40.0	23.0	41.0
49	34.81728676252548	38.0	34.0	40.0	24.0	41.0
50	34.67089913373755	38.0	34.0	40.0	23.0	41.0
51	32.8298769061875	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	18.0
10	21.0
11	14.0
12	6.0
13	9.0
14	8.0
15	20.0
16	48.0
17	96.0
18	171.0
19	257.0
20	408.0
21	690.0
22	1208.0
23	1724.0
24	2588.0
25	3429.0
26	4436.0
27	4814.0
28	5019.0
29	5597.0
30	6597.0
31	8278.0
32	10896.0
33	16075.0
34	22014.0
35	27837.0
36	34467.0
37	58025.0
38	62033.0
39	40177.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96422014296801	19.44989495069307	23.35312340296662	14.232761503372304
2	34.049540369849275	24.906780569979244	25.76578736506054	15.277891695110949
3	31.300592440235462	24.97649797787971	28.616314504375477	15.106595077509352
4	28.44312510646889	24.06386240749036	30.20404171687792	17.288970769162823
5	23.725370196281318	36.53349905676448	23.82505662567746	15.916074121276743
6	25.2307614655167	35.53032549511978	26.425105837965386	12.813807201398134
7	46.03336340750929	43.49861511574352	7.585317072247425	2.8827044044997696
8	77.88223120942352	7.398878212205909	11.139958485018644	3.578932093351925
9	71.59536142639924	9.291027590427579	12.938099774759143	6.17551120841404
10	40.44303677672133	27.156034499075695	19.176388196622018	13.224540527580963
11	32.05991280592062	25.515624901417695	26.862338088418074	15.562124204243613
12	30.055142999552043	23.17741029798671	29.174684694347526	17.59276200811372
13	23.376783156778995	25.379660182842578	31.651387723426943	19.592168936951488
14	19.604472008933925	28.904963500886456	30.61193587260327	20.878628617576357
15	18.408234856180243	26.58315299343205	36.548641299204405	18.459970851183304
16	22.399162129251657	23.56606118727799	35.16501889625671	18.869757787213636
17	21.091881865271898	23.910231739402008	32.46055130381016	22.537335091515928
18	22.188432588629436	26.47431812589513	30.843801459964542	20.493447825510895
19	24.950945443762343	26.438670763484485	28.985091200464364	19.62529259228881
20	27.1667602541373	24.652832545726415	30.716669716146043	17.463737483990233
21	24.931702177328276	25.994813781964325	29.193612497397424	19.879871543309967
22	22.412096128002425	26.216900004416487	29.781005318712655	21.589998548868433
23	24.105503574200142	26.44876559177776	29.64819523397919	19.797535600042902
24	22.176760443415333	25.300478873417166	31.096803094064875	21.42595758910263
25	22.382758033275078	27.28537448658334	29.68541991331066	20.646447566830915
26	21.49252036316145	28.504009539612735	30.279752929077524	19.723717168148294
27	22.43891051565645	28.288863511612206	30.155460355716514	19.116765617014835
28	20.87326574004555	28.62167738190628	31.7482349823656	18.756821895682567
29	22.589071086518988	27.123226307122533	30.593323532937532	19.694379073420947
30	22.09316264661161	26.821012385092462	30.452311400215777	20.633513568080154
31	23.272049313236213	27.711880981974424	28.230502785541685	20.78556691924768
32	24.780910679697406	28.311892338656254	28.882250137226574	18.024946844419766
33	23.481201536937608	28.330189214937825	28.80527707149031	19.38333217663426
34	23.061635235998157	27.888540477106822	29.085408556628835	19.96441573026619
35	22.713048196495834	26.543089143643094	28.930516035003816	21.813346624857253
36	23.964806904862552	28.92452223070468	27.95163315393982	19.159037710492942
37	22.777087263481327	29.3570225303949	29.501504760342467	18.36438544578131
38	21.857826962024518	28.607481529618855	28.38634169731919	21.148349811037434
39	24.081843820387768	26.951298762752607	28.090752506356587	20.876104910503038
40	22.686864735610136	26.504918074159136	30.643166747635604	20.165050442595128
41	20.504173580572502	28.570256850287386	29.505921247720778	21.41964832141933
42	22.403578616629968	27.99800627141208	28.983829346927703	20.614585765030252
43	23.724739269512988	26.99704095345653	28.555114607847464	20.72310516918301
44	22.025968945784463	28.626093869284592	28.59833309147807	20.749604093452874
45	21.66665615122053	27.702732543833637	28.187915228679405	22.442696076266426
46	21.330687647084805	29.70529410651306	28.016618611077813	20.94739963532433
47	22.28527984756809	27.796425168930643	30.303412682889896	19.61488230061137
48	22.68244824823183	27.06202641059452	28.859536773566692	21.395988567606956
49	20.737616484854605	28.57309602074487	29.127365186722777	21.561922307677747
50	19.88428803068828	28.486974516867825	29.103074506142075	22.52566294630182
51	20.310479062695194	28.25100790551241	28.15857713395206	23.279935897840335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	354.0
1	796.0
2	1238.0
3	3496.0
4	5754.0
5	3990.0
6	2226.0
7	1966.5
8	1707.0
9	1642.0
10	1577.0
11	1547.5
12	1518.0
13	1514.0
14	1510.0
15	1624.5
16	1739.0
17	1711.5
18	1684.0
19	1699.0
20	1714.0
21	1872.0
22	2030.0
23	2188.0
24	2346.0
25	2984.5
26	4229.0
27	4835.0
28	5689.0
29	6543.0
30	7216.5
31	7890.0
32	9009.5
33	10129.0
34	10620.0
35	11111.0
36	11693.5
37	12276.0
38	13474.5
39	14673.0
40	15761.5
41	16850.0
42	17843.5
43	18837.0
44	19469.5
45	20102.0
46	21570.5
47	23039.0
48	20971.0
49	18903.0
50	18439.0
51	17975.0
52	16639.0
53	15303.0
54	15040.5
55	14778.0
56	14804.0
57	14830.0
58	14752.0
59	14674.0
60	14420.5
61	14167.0
62	12523.0
63	10879.0
64	8856.0
65	6833.0
66	5702.0
67	4571.0
68	3826.5
69	3082.0
70	2597.0
71	2112.0
72	1760.5
73	1409.0
74	1200.5
75	761.0
76	530.0
77	403.5
78	277.0
79	220.0
80	163.0
81	126.5
82	90.0
83	70.5
84	51.0
85	45.5
86	40.0
87	29.0
88	18.0
89	12.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.30225537450893
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.87650688698564	21.925642593930245
2	8.207381392271499	5.302338593782932
3	4.267202695796049	4.135208136431908
4	3.0649838699714063	3.960235667462682
5	2.4961962335677956	4.031638410079713
6	1.8176757114312436	3.5229015011216545
7	1.5278860049523035	3.4547914740575267
8	1.42396170546867	3.6797739722856195
9	1.2101176262187145	3.518057573578032
>10	8.006162193884421	41.51559708906124
>50	0.0669511816009781	1.5225871700905245
>100	0.02997814101536333	1.9296218153065834
>500	0.002997814101536333	0.5852131734946195
>1k	0.001998542734357555	0.9163928293167265
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1787	0.563733067502855	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.33186748014157996	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	704	0.22208622245215998	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	572	0.18044505574237998	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	537	0.169403837296605	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCG	469	0.14795232717338497	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	404	0.12744720720266	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	362	0.11419774506772999	No Hit
GAATCTATCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	336	0.10599569707943998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.3092676833E-4	0.0	0.0	0.022397900275714998	0.0
2	6.3092676833E-4	0.0	0.0	0.140381205953425	0.0
3	6.3092676833E-4	0.0	0.0	0.17918320220571998	0.0
4	6.3092676833E-4	0.0	0.0	0.25994182855195996	0.0
5	6.3092676833E-4	0.0	0.0	0.6063206243651299	0.0
6	6.3092676833E-4	0.0	0.0	0.705060663608775	0.0
7	6.3092676833E-4	0.0	0.0	0.870363476911235	0.0
8	6.3092676833E-4	0.0	0.0	1.1858268610762348	0.0
9	6.3092676833E-4	0.0	0.0	1.305702947058935	0.0
10	6.3092676833E-4	0.0	0.0	2.00887083036272	0.0
11	6.3092676833E-4	0.0	0.0	2.310138362240295	0.0
12	6.3092676833E-4	0.0	0.0	2.89406108632971	0.0
13	6.3092676833E-4	0.0	0.0	3.0085742947816048	0.0
14	6.3092676833E-4	0.0	0.0	3.0688278011571195	0.0
15	6.3092676833E-4	0.0	0.0	3.1924894477497996	0.0
16	6.3092676833E-4	0.0	0.0	3.3644169921197244	0.0
17	6.3092676833E-4	0.0	0.0	3.5559032663078796	0.0
18	6.3092676833E-4	0.0	0.0	3.7514905644901795	0.0
19	6.3092676833E-4	0.0	0.0	3.8868243562969647	0.0
20	6.3092676833E-4	0.0	0.0	4.030044732707875	0.0
21	6.3092676833E-4	0.0	0.0	4.203549593998624	0.0
22	6.3092676833E-4	0.0	0.0	4.37926269897853	0.0
23	6.3092676833E-4	0.0	0.0	4.573588143624169	0.0
24	6.3092676833E-4	0.0	0.0	4.7341590061641545	0.0
25	6.3092676833E-4	0.0	0.0	4.8748556755017445	0.0
26	6.3092676833E-4	0.0	0.0	5.01839151529682	0.0
27	6.3092676833E-4	0.0	0.0	5.1512016000302845	0.0
28	6.3092676833E-4	0.0	0.0	5.294106513057029	0.0
29	6.3092676833E-4	0.0	0.0	5.443951620535405	0.0
30	6.3092676833E-4	0.0	0.0	5.63985438210187	0.0
31	6.3092676833E-4	0.0	0.0	5.821245827996744	0.0
32	6.3092676833E-4	0.0	0.0	6.003268200659949	0.0
33	6.3092676833E-4	0.0	0.0	6.174564818261544	0.0
34	6.3092676833E-4	0.0	0.0	6.34523050909481	0.0
35	6.3092676833E-4	0.0	0.0	6.522205467611374	0.0
36	6.3092676833E-4	0.0	0.0	6.720316472866994	0.0
37	6.3092676833E-4	0.0	0.0	6.910225430134324	0.0
38	6.3092676833E-4	0.0	0.0	7.103919948011634	0.0
39	6.3092676833E-4	0.0	0.0	7.290043344668984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAG	30	2.1605101E-6	45.000004	2
GTAGACG	30	2.1605101E-6	45.000004	1
CGGGCAT	30	2.1605101E-6	45.000004	6
GATCGTA	25	3.8843922E-5	45.0	10
GTTTCGA	20	7.025547E-4	45.0	11
GCGAAGA	20	7.025547E-4	45.0	1
CGTTGCG	20	7.025547E-4	45.0	11
CGTTGCC	20	7.025547E-4	45.0	43
CACGCTT	20	7.025547E-4	45.0	19
CACGCAA	20	7.025547E-4	45.0	18
ATAGAAC	20	7.025547E-4	45.0	37
CGCGAGA	20	7.025547E-4	45.0	16
CGACAAT	20	7.025547E-4	45.0	20
CGACAAG	20	7.025547E-4	45.0	2
CGTTATT	175	0.0	45.0	1
TATGACA	20	7.025547E-4	45.0	33
ACGATCC	20	7.025547E-4	45.0	22
CGGGCAA	25	3.8843922E-5	45.0	6
CATACGA	35	1.2082091E-7	45.0	18
GCGATAC	20	7.025547E-4	45.0	9
>>END_MODULE