FastQCFastQC Report
Sat 18 Jun 2016
SRR3551099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684806
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62040.9059500062791506No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC35210.5141602147177449No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35100.5125539203803705No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG27470.4011355040697657No Hit
GAATCTATCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC21660.31629395770480984No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC21050.30738632547027916No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG18180.26547664594060216No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC13960.2038533540886032No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC9010.13157010890675608TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT8580.12529095831520168No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG7740.11302471064797914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC351.210974E-745.06
CGACGGT351.210974E-745.028
CACGCTA207.0312875E-445.038
CACGACG406.8084773E-945.026
TGCGGAA207.0312875E-445.02
CTCGCGT351.210974E-745.012
GGTTCGC351.210974E-745.010
TGTACGA207.0312875E-445.01
AATCGTT406.8084773E-945.022
AGGATCG253.8891492E-544.9999967
TTATTCG253.8891492E-544.99999616
CGTTTTA14300.044.0559431
CGTTTTT27050.043.4195981
CGTTATT5400.042.5000041
AGGCGAT2650.042.452837
TAGGCGA850.042.352946
CGGTTTA800.042.18751
ACGGGCG556.002665E-1140.9090925
TACGGCT3700.040.7432447
TTAATCG451.9263098E-840.020