##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684806
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67949901139885	31.0	31.0	34.0	30.0	34.0
2	31.846296615391804	31.0	31.0	34.0	30.0	34.0
3	31.457697508491457	31.0	31.0	34.0	28.0	34.0
4	35.41940783229119	37.0	35.0	37.0	33.0	37.0
5	31.826983116386245	37.0	35.0	37.0	0.0	37.0
6	33.72646413728852	37.0	35.0	37.0	19.0	37.0
7	20.934007587550344	32.0	0.0	37.0	0.0	37.0
8	28.067382587185275	35.0	17.0	37.0	17.0	37.0
9	34.57316962760256	35.0	32.0	39.0	28.0	39.0
10	36.08356089169779	37.0	35.0	39.0	32.0	39.0
11	36.49850468599866	37.0	35.0	39.0	32.0	39.0
12	36.940266294395784	39.0	37.0	39.0	33.0	39.0
13	36.80241703489747	39.0	37.0	39.0	33.0	39.0
14	38.24584626887031	40.0	37.0	41.0	34.0	41.0
15	38.31799370916727	40.0	38.0	41.0	34.0	41.0
16	38.3674675747584	40.0	38.0	41.0	34.0	41.0
17	38.264206213146494	40.0	37.0	41.0	33.0	41.0
18	38.0966302865337	40.0	37.0	41.0	33.0	41.0
19	38.0024488687307	40.0	37.0	41.0	33.0	41.0
20	37.964065735405356	40.0	37.0	41.0	34.0	41.0
21	37.922585374544035	40.0	36.0	41.0	33.0	41.0
22	37.88834356007395	39.0	36.0	41.0	33.0	41.0
23	37.85113740241762	39.0	36.0	41.0	33.0	41.0
24	37.688519084236994	39.0	36.0	41.0	33.0	41.0
25	37.558216487589185	39.0	36.0	41.0	33.0	41.0
26	37.37875106234466	39.0	35.0	41.0	33.0	41.0
27	37.356134438074434	39.0	35.0	41.0	33.0	41.0
28	37.221878897089105	39.0	35.0	41.0	32.0	41.0
29	37.15561925567241	39.0	35.0	41.0	32.0	41.0
30	36.97212933297897	39.0	35.0	40.0	31.0	41.0
31	36.7453001287956	39.0	35.0	40.0	31.0	41.0
32	36.63661241285853	39.0	35.0	40.0	31.0	41.0
33	36.418901119441124	39.0	35.0	40.0	30.0	41.0
34	36.10692955377143	39.0	35.0	40.0	29.0	41.0
35	35.904954103789976	39.0	35.0	40.0	27.0	41.0
36	35.5838091371863	39.0	35.0	40.0	25.0	41.0
37	35.42063299679033	39.0	35.0	40.0	25.0	41.0
38	35.29490103766614	39.0	35.0	40.0	24.0	41.0
39	35.053169802834674	38.0	34.0	40.0	23.0	41.0
40	34.82350621927962	38.0	34.0	40.0	22.0	41.0
41	34.67787081304778	38.0	34.0	40.0	21.0	41.0
42	34.422767907991464	38.0	33.0	40.0	19.0	41.0
43	34.276665216134205	38.0	33.0	40.0	18.0	41.0
44	33.99656253011802	38.0	33.0	40.0	17.0	41.0
45	33.98369611247565	38.0	33.0	40.0	18.0	41.0
46	33.58179542819426	38.0	33.0	40.0	15.0	41.0
47	33.369095773109464	38.0	32.0	40.0	14.0	41.0
48	33.21082467151281	38.0	32.0	40.0	13.0	41.0
49	33.04994699228687	37.0	31.0	40.0	13.0	41.0
50	32.96284057090621	37.0	31.0	40.0	12.0	41.0
51	30.880829023110195	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	27.0
10	21.0
11	11.0
12	11.0
13	14.0
14	8.0
15	24.0
16	42.0
17	100.0
18	208.0
19	445.0
20	823.0
21	1481.0
22	2526.0
23	4406.0
24	7348.0
25	10891.0
26	13661.0
27	15214.0
28	16161.0
29	17058.0
30	18987.0
31	22143.0
32	27100.0
33	35524.0
34	46116.0
35	59168.0
36	75428.0
37	117343.0
38	117180.0
39	75310.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.516154940231246	19.091392306726284	23.007975981518854	18.384476771523612
2	33.165305210526775	27.672070630222283	23.213143576428948	15.949480582821995
3	26.92163912115256	27.24800892515545	31.145609121415408	14.684742832276587
4	24.648440580251926	22.94941925158366	35.690545935637246	16.71159423252717
5	22.316539282658155	34.40346609112654	26.585631551125427	16.69436307508988
6	22.414085156964163	34.01810147691463	29.797052011810642	13.770761354310565
7	44.16637704693008	41.72247322599393	10.997421167454725	3.113728559621265
8	74.29257337114453	6.3597573619390015	14.734976037009021	4.612693229907448
9	68.41280596256458	7.2163503240333755	15.815720072546094	8.555123640855951
10	33.88229659202752	30.65057841198821	22.245716305055737	13.221408690928527
11	23.447078442653833	24.753433819213033	33.233353679728275	18.566134058404863
12	22.601145433889304	22.14729427020207	35.66309290514394	19.588467390764684
13	19.391769347815295	23.58726413027923	38.67985969749097	18.341106824414506
14	17.290298274255775	27.646808001098123	33.58717651422447	21.47571721042164
15	16.629089114289304	26.614982929472	38.994109280584574	17.761818675654126
16	18.86913958113685	27.231361874749926	33.45984118129806	20.43965736281516
17	19.137828815752197	26.085197851654335	33.35192740717809	21.425045925415372
18	19.32810168135209	26.837089628303488	33.798039152694344	20.03676953765008
19	19.500267228967036	27.592632073901225	31.66546437969294	21.241636317438807
20	20.696080349763292	27.291816952538383	33.60718218006267	18.404920517635652
21	20.713457533958525	28.038451765901584	33.27307295788881	17.97501774225109
22	19.73551633601341	23.83521756526666	32.913409053074886	23.515857045645046
23	18.705151531966717	26.794011734710267	34.633166181371074	19.867670551951942
24	20.8488243385718	24.20524936989454	33.48919255964463	21.45673373188903
25	18.57445758360762	29.469952074018043	31.1882489347348	20.767341407639535
26	18.885494578026478	27.520786908993205	31.886110810944995	21.707607702035318
27	20.487700166178453	27.652795098173787	30.609690919764137	21.249813815883623
28	17.28898403343429	27.429958265552578	33.65595511721568	21.625102583797457
29	19.3162735139587	27.626948362017856	32.01592859875644	21.04084952526701
30	18.468150103825025	29.71454689357278	31.731468474283226	20.08583452831897
31	19.57809949095072	29.09510138637804	29.736742960780134	21.590056161891106
32	19.48508044614095	28.758801762835024	31.292219986390307	20.46389780463372
33	17.74663189282804	27.374760151050076	29.708705823255055	25.169902132866827
34	18.077090446053333	27.923090627126513	30.17146461917682	23.82835430764333
35	17.852209238820922	26.76144776768895	29.622404009310664	25.763938984179465
36	18.153170386941703	29.34933397195702	28.524428816336307	23.97306682476497
37	17.399088208923402	27.942658212690898	32.657570173158526	22.00068340522717
38	17.94201569495594	28.269466096967612	28.65453865766363	25.133979550412818
39	20.34809858558482	25.009126672371444	30.49973277103296	24.143041971010767
40	19.836128772236226	25.897991547971248	30.62706810395937	23.638811575833156
41	17.970636939512797	27.525021684973556	28.86248076097464	25.64186061453901
42	18.530357502708796	27.017140620847364	31.376185372207605	23.076316504236235
43	21.36984781091287	25.458597033320384	29.719511803342847	23.4520433524239
44	19.74281767391057	26.20815238184245	29.747548940867922	24.30148100337906
45	18.794811961343797	25.757951887103793	29.67030078591601	25.7769353656364
46	21.183371641019498	27.284953694915053	28.161552322847637	23.370122341217805
47	18.79305964024848	25.85973253739015	32.05491774312725	23.292290079234117
48	19.455290987520556	25.563590272281495	30.06559521966805	24.915523520529902
49	18.614468915284036	24.249057397277475	33.013145328750035	24.123328358688447
50	18.536490626542406	24.660560801161203	32.53943452598254	24.263514046313848
51	18.66557828056413	24.032645742005766	29.814283169247936	27.487492808182175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	872.0
2	1312.0
3	9587.0
4	17862.0
5	12412.0
6	6962.0
7	5977.0
8	4992.0
9	4842.0
10	4692.0
11	4535.5
12	4379.0
13	4284.5
14	4190.0
15	4045.0
16	3900.0
17	3772.0
18	3644.0
19	3616.5
20	3589.0
21	3867.5
22	4146.0
23	4503.5
24	4861.0
25	5642.5
26	7498.5
27	8573.0
28	9986.5
29	11400.0
30	12870.5
31	14341.0
32	16500.0
33	18659.0
34	20446.0
35	22233.0
36	24190.5
37	26148.0
38	28797.5
39	31447.0
40	34227.0
41	37007.0
42	40081.0
43	43155.0
44	50276.5
45	57398.0
46	66973.5
47	76549.0
48	67862.0
49	59175.0
50	54434.0
51	49693.0
52	42573.0
53	35453.0
54	31304.5
55	27156.0
56	24767.5
57	22379.0
58	20728.5
59	19078.0
60	17690.5
61	16303.0
62	14305.5
63	12308.0
64	10133.5
65	7959.0
66	6802.0
67	5645.0
68	4894.5
69	4144.0
70	3474.5
71	2805.0
72	2330.5
73	1856.0
74	1466.0
75	826.0
76	576.0
77	489.5
78	403.0
79	325.0
80	247.0
81	182.5
82	118.0
83	94.0
84	70.0
85	54.5
86	39.0
87	27.5
88	16.0
89	10.5
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.08089763317483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13127092991824	35.22227826741492
2	7.640325255429811	6.888654414483833
3	3.2404081173892427	4.382415198891996
4	1.9770641385293992	3.565113041730593
5	1.482073516641096	3.340660224426834
6	1.1637328742457445	3.1477273545739632
7	0.948219219847579	2.9922601508630327
8	0.808749762522476	2.9167332204104155
9	0.6861336252232262	2.783836774923066
>10	3.856115781389941	26.566498263264243
>50	0.034539381854064403	1.074216883076198
>100	0.024670986411678322	2.2710924148118714
>500	0.003876869293263719	1.119975544424853
>1k	0.002467098641167821	2.7428199494121484
>5k	3.5244266302397444E-4	0.9857182972920678
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6204	0.9059500062791506	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	3521	0.5141602147177449	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3510	0.5125539203803705	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	2747	0.4011355040697657	No Hit
GAATCTATCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	2166	0.31629395770480984	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	2105	0.30738632547027916	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	1818	0.26547664594060216	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	1396	0.2038533540886032	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	901	0.13157010890675608	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	858	0.12529095831520168	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	774	0.11302471064797914	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.460267579431255E-4	0.0	0.0	0.10557734599287974	0.0
2	1.460267579431255E-4	0.0	0.0	0.5753454262959145	0.0
3	1.460267579431255E-4	0.0	0.0	0.7778845395630295	0.0
4	1.460267579431255E-4	0.0	0.0	1.1806263379701696	0.0
5	1.460267579431255E-4	0.0	0.0	2.645274720139718	0.0
6	1.460267579431255E-4	0.0	0.0	3.3961443094832697	0.0
7	1.460267579431255E-4	0.0	0.0	4.066845208716045	0.0
8	1.460267579431255E-4	0.0	0.0	5.299457072513968	0.0
9	1.460267579431255E-4	0.0	0.0	5.760463547340414	0.0
10	1.460267579431255E-4	0.0	0.0	7.727590003592258	0.0
11	1.460267579431255E-4	0.0	0.0	8.60229028367158	0.0
12	1.460267579431255E-4	0.0	0.0	10.080227100813953	0.0
13	1.460267579431255E-4	0.0	0.0	10.504434832638733	0.0
14	1.460267579431255E-4	0.0	0.0	10.724643183616966	0.0
15	1.460267579431255E-4	0.0	0.0	11.020493395209739	0.0
16	1.460267579431255E-4	0.0	0.0	11.496540626104327	0.0
17	1.460267579431255E-4	0.0	0.0	12.042972754327502	0.0
18	1.460267579431255E-4	0.0	0.0	12.670887813482942	0.0
19	1.460267579431255E-4	0.0	0.0	13.052747785504216	0.0
20	1.460267579431255E-4	0.0	0.0	13.415332225476996	0.0
21	1.460267579431255E-4	0.0	0.0	13.856187007707293	0.0
22	1.460267579431255E-4	0.0	0.0	14.315441161438422	0.0
23	1.460267579431255E-4	0.0	0.0	14.799957944293713	0.0
24	1.460267579431255E-4	0.0	0.0	15.171888096774852	0.0
25	1.460267579431255E-4	0.0	0.0	15.524104636933671	0.0
26	1.460267579431255E-4	0.0	0.0	15.864054929425269	0.0
27	1.460267579431255E-4	0.0	0.0	16.209262185202817	0.0
28	1.460267579431255E-4	0.0	0.0	16.571116491385883	0.0
29	1.460267579431255E-4	0.0	0.0	16.944506911446453	0.0
30	1.460267579431255E-4	0.0	0.0	17.37134312491421	0.0
31	1.460267579431255E-4	0.0	0.0	17.765761398118592	0.0
32	1.460267579431255E-4	0.0	0.0	18.15068793205667	0.0
33	1.460267579431255E-4	0.0	0.0	18.50538692710052	0.0
34	1.460267579431255E-4	0.0	0.0	18.86548891218827	0.0
35	1.460267579431255E-4	0.0	0.0	19.24486642932451	0.0
36	1.460267579431255E-4	0.0	0.0	19.611977698793527	0.0
37	1.460267579431255E-4	0.0	0.0	19.968721068448584	0.0
38	1.460267579431255E-4	0.0	0.0	20.32458827755598	0.0
39	1.460267579431255E-4	0.0	0.0	20.663954463015802	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	35	1.210974E-7	45.0	6
CGACGGT	35	1.210974E-7	45.0	28
CACGCTA	20	7.0312875E-4	45.0	38
CACGACG	40	6.8084773E-9	45.0	26
TGCGGAA	20	7.0312875E-4	45.0	2
CTCGCGT	35	1.210974E-7	45.0	12
GGTTCGC	35	1.210974E-7	45.0	10
TGTACGA	20	7.0312875E-4	45.0	1
AATCGTT	40	6.8084773E-9	45.0	22
AGGATCG	25	3.8891492E-5	44.999996	7
TTATTCG	25	3.8891492E-5	44.999996	16
CGTTTTA	1430	0.0	44.055943	1
CGTTTTT	2705	0.0	43.419598	1
CGTTATT	540	0.0	42.500004	1
AGGCGAT	265	0.0	42.45283	7
TAGGCGA	85	0.0	42.35294	6
CGGTTTA	80	0.0	42.1875	1
ACGGGCG	55	6.002665E-11	40.909092	5
TACGGCT	370	0.0	40.743244	7
TTAATCG	45	1.9263098E-8	40.0	20
>>END_MODULE