FastQCFastQC Report
Sat 18 Jun 2016
SRR3551098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566523
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78571.3868810268956424No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.791847815534409No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC8860.15639259129814675No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8010.14138878739256835No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG6080.10732132675990208No Hit
GAATCTATCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC5970.10537965801918016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG700.045.05
AGATACG351.2104647E-745.01
CCGTGCC253.888292E-545.012
CGTTCTA406.8048394E-945.01
TTGACGA207.0302555E-445.01
CGTGTAC207.0302555E-445.018
ATGCGAA253.888292E-545.01
CGCACGG207.0302555E-445.02
TCGGTTA207.0302555E-445.01
TGCGTAA207.0302555E-445.01
CGTTATT7350.044.3877561
CGTTTTA17400.043.836211
CGTTTTT30350.043.369031
TAGGCGA1050.042.8571436
AGGCGAT2850.041.8421067
CTCGTCC1350.041.66666437
CCTCGTC1350.041.66666436
TCCTCGT1400.040.1785735
TCGTCCC1400.040.1785738
CGGTTTT3250.040.1538471