##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566523
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72845586145664	31.0	31.0	34.0	30.0	34.0
2	31.910600275716963	33.0	31.0	34.0	30.0	34.0
3	31.350338821195255	31.0	31.0	34.0	28.0	34.0
4	35.381567915159664	37.0	35.0	37.0	33.0	37.0
5	31.824337229026888	37.0	35.0	37.0	0.0	37.0
6	33.78676946920072	37.0	35.0	37.0	19.0	37.0
7	21.049071264538245	32.0	0.0	37.0	0.0	37.0
8	28.26296196270937	35.0	17.0	37.0	17.0	37.0
9	34.8905322467049	37.0	32.0	39.0	32.0	39.0
10	36.274008292690674	37.0	35.0	39.0	32.0	39.0
11	36.734651549892945	38.0	37.0	39.0	33.0	39.0
12	37.148458226762195	39.0	37.0	39.0	34.0	39.0
13	37.005713801557924	39.0	37.0	39.0	33.0	39.0
14	38.440845296660505	40.0	38.0	41.0	34.0	41.0
15	38.57512228100183	40.0	38.0	41.0	34.0	41.0
16	38.60545114673191	40.0	38.0	41.0	34.0	41.0
17	38.55976897672292	40.0	38.0	41.0	34.0	41.0
18	38.39943656303451	40.0	38.0	41.0	34.0	41.0
19	38.25776888140464	40.0	37.0	41.0	34.0	41.0
20	38.14746974085783	40.0	37.0	41.0	34.0	41.0
21	38.065806683929864	40.0	37.0	41.0	34.0	41.0
22	37.99054936869289	39.0	36.0	41.0	34.0	41.0
23	37.88908129060956	39.0	36.0	41.0	33.0	41.0
24	37.73469744388136	39.0	35.0	41.0	33.0	41.0
25	37.581245598148705	39.0	35.0	41.0	33.0	41.0
26	37.46753618123183	39.0	35.0	41.0	33.0	41.0
27	37.41953636480778	39.0	35.0	41.0	33.0	41.0
28	37.34762401526505	39.0	35.0	41.0	33.0	41.0
29	37.32961415511815	39.0	35.0	41.0	33.0	41.0
30	37.152588685719735	39.0	35.0	41.0	33.0	41.0
31	36.983047466740096	39.0	35.0	41.0	32.0	41.0
32	36.79763222322836	39.0	35.0	41.0	31.0	41.0
33	36.48792193785601	39.0	35.0	41.0	30.0	41.0
34	36.15478630170355	39.0	35.0	41.0	29.0	41.0
35	35.83256637418075	39.0	35.0	41.0	26.0	41.0
36	35.50703501887831	39.0	35.0	40.0	25.0	41.0
37	35.365483837372885	39.0	35.0	40.0	24.0	41.0
38	35.24645248295303	39.0	35.0	40.0	23.0	41.0
39	35.1498279858011	39.0	35.0	40.0	23.0	41.0
40	35.06748004935369	39.0	35.0	40.0	22.0	41.0
41	34.93359669422071	39.0	35.0	40.0	22.0	41.0
42	34.79545578908535	38.0	34.0	40.0	21.0	41.0
43	34.70149843872182	38.0	34.0	40.0	21.0	41.0
44	34.58493829906288	38.0	34.0	40.0	20.0	41.0
45	34.540256971032065	38.0	34.0	40.0	20.0	41.0
46	34.423366747687204	38.0	34.0	40.0	20.0	41.0
47	34.37511098402007	38.0	34.0	40.0	20.0	41.0
48	34.228410850044924	38.0	33.0	40.0	19.0	41.0
49	34.08398599880323	38.0	33.0	40.0	18.0	41.0
50	33.9211982567345	38.0	33.0	40.0	18.0	41.0
51	32.16301191655061	36.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	27.0
10	23.0
11	20.0
12	11.0
13	8.0
14	16.0
15	12.0
16	23.0
17	77.0
18	171.0
19	356.0
20	624.0
21	1129.0
22	2232.0
23	3889.0
24	6509.0
25	9531.0
26	11670.0
27	11759.0
28	10949.0
29	10900.0
30	11741.0
31	14574.0
32	18905.0
33	26374.0
34	35851.0
35	48621.0
36	62405.0
37	102850.0
38	106287.0
39	68960.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.742414694549026	20.39158163040159	23.541144843192598	20.324858831856783
2	35.915399727813345	24.185778865112272	25.231808770341186	14.667012636733196
3	28.40220079325994	23.446003074897224	34.5121027742916	13.639693357551238
4	24.713383216568435	24.29821913673408	35.1976883551065	15.790709291590984
5	20.6729470824662	35.2598217548096	29.537370945221998	14.529860217502202
6	22.60420141812424	32.7829585030087	31.5828307059025	13.030009372964557
7	45.18686796476048	41.626553555636754	10.652347742280543	2.5342307373222273
8	78.74649396405795	3.8240283271817734	14.755093791425944	2.674383917334336
9	72.68036778736257	7.270137311283037	15.943042030067623	4.106452871286779
10	30.819048829438522	35.4354545181749	22.334839009184094	11.410657643202482
11	24.20237130707844	25.713519133380284	34.7560469742623	15.328062585278973
12	23.918005094232715	22.806664513179516	36.40981919533717	16.865511197250598
13	20.318680794954485	23.83098303158036	37.901020788211596	17.949315385253556
14	17.6640665957075	27.81475774152153	35.84391101508677	18.677264647684208
15	17.616936999909978	26.603862508671316	38.70663680027113	17.07256369114758
16	21.009561835971354	26.10503015764585	36.10956660188553	16.775841404497257
17	20.223362511319046	25.417326392750162	35.46051969646422	18.89879139946657
18	20.47595596295296	25.896565541028345	35.35408094640465	18.27339754961405
19	21.122531653613358	26.67958758955947	33.91265667942873	18.285224077398446
20	22.055768256540333	26.522488936901063	34.728863611892194	16.692879194666414
21	21.54140255558909	25.82225258286071	35.63703503652985	16.999309825020344
22	21.088640708320757	24.210844043401593	34.835125846611696	19.86538940166595
23	19.788958259417537	26.269542454587015	35.235462637880545	18.706036648114903
24	19.90298716909993	25.254932279889786	36.19199926569618	18.650081285314098
25	19.186864434453675	27.856415361777014	34.11176598302276	18.844954220746555
26	19.494354156848	28.58771841566891	33.324507566330055	18.59341986115303
27	19.133556801753855	28.03822616204461	34.55799676270866	18.270220273492868
28	18.027158650222496	27.867359312861083	35.972237667314474	18.13324436960194
29	19.48482232848446	26.310670528822307	35.291064970001216	18.91344217269202
30	20.247192082227905	27.30586401611232	34.0298628652323	18.417081036427472
31	20.77197218824302	27.376293636798504	33.37834474505007	18.47338942990841
32	21.662315563534047	27.59464311245969	33.1584066313283	17.584634692677966
33	21.118648316131914	27.51732939351094	33.19106196924044	18.172960321116708
34	21.23232419513418	27.04620995087578	33.61769954617906	18.103766307810982
35	21.8125301179299	26.263540933024785	32.75842287073959	19.165506078305736
36	21.867073358010177	28.00451173209208	32.20116394215239	17.92725096774535
37	21.156952144926155	28.336537086755527	32.85921312991705	17.647297638401266
38	21.159070329006944	27.964442749897177	31.823068083731815	19.05341883736406
39	22.185154000808442	27.0769236200472	31.54029050894668	19.19763187019768
40	22.709051530123226	26.908351470284526	32.26488597991609	18.117711019676165
41	20.556799988703016	28.141311120642936	31.79782639010243	19.504062500551612
42	21.048748241465926	28.30441129486358	31.106062772385236	19.54077769128526
43	21.70220803038888	27.874066895783578	30.958848978770497	19.46487609505704
44	21.546521500450996	28.082884543081217	30.283148256999276	20.08744569946851
45	20.523438589430615	28.244749109921397	29.980600964126786	21.2512113365212
46	21.385539510311144	28.826896701457837	29.45952768025305	20.328036107977965
47	21.022977001816344	27.42342323259603	31.74134148128143	19.812258284306196
48	20.941956460726217	27.07533498198661	31.278694774969416	20.70401378231775
49	20.415058170630317	26.70783004396997	32.15191616227408	20.72519562312563
50	20.088504791508907	27.52350743041324	31.88961436693656	20.498373411141298
51	20.45653927554574	26.742427050622837	31.00527251320776	21.795761160623663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	589.0
2	938.0
3	10785.5
4	20633.0
5	14110.0
6	7587.0
7	6666.5
8	5746.0
9	5706.5
10	5667.0
11	5550.0
12	5433.0
13	5235.0
14	5037.0
15	4762.5
16	4488.0
17	4406.5
18	4325.0
19	4197.0
20	4069.0
21	4127.0
22	4185.0
23	4156.0
24	4127.0
25	4976.0
26	6851.5
27	7878.0
28	8876.5
29	9875.0
30	11440.0
31	13005.0
32	14745.5
33	16486.0
34	18415.0
35	20344.0
36	22088.5
37	23833.0
38	25748.5
39	27664.0
40	29856.0
41	32048.0
42	34011.5
43	35975.0
44	39086.5
45	42198.0
46	46051.5
47	49905.0
48	47344.5
49	44784.0
50	41549.0
51	38314.0
52	33981.0
53	29648.0
54	26755.5
55	23863.0
56	21486.5
57	19110.0
58	17750.0
59	16390.0
60	15184.0
61	13978.0
62	11680.0
63	9382.0
64	7519.0
65	5656.0
66	4407.5
67	3159.0
68	2484.0
69	1809.0
70	1512.5
71	1216.0
72	1046.0
73	876.0
74	667.5
75	329.5
76	200.0
77	135.5
78	71.0
79	51.5
80	32.0
81	27.5
82	23.0
83	20.5
84	18.0
85	17.0
86	16.0
87	9.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	566523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.33489751428056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18220612322882	35.29894713131604
2	8.595653769128246	7.965574729215934
3	3.8151392473248786	5.303222580825231
4	2.336277026850677	4.330046264163769
5	1.7014093618235053	3.9417314204964797
6	1.289150333468366	3.5839589149054407
7	1.0234174358958041	3.319395940459192
8	0.8341562702254872	3.092043623343397
9	0.6911545852782291	2.8822119187852646
>10	3.485892592821388	24.87058978302274
>50	0.02681518691117711	0.8551410590458771
>100	0.01617423971934174	1.5525070145912245
>500	0.0017025515494043936	0.5703569977385444
>1k	4.256378873510984E-4	0.8847238906829565
>5k	4.256378873510984E-4	1.5495487314079335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7857	1.3868810268956424	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4486	0.791847815534409	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	886	0.15639259129814675	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	801	0.14138878739256835	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG	608	0.10732132675990208	No Hit
GAATCTATCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	597	0.10537965801918016	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06372203776369186	0.0
2	0.0	0.0	0.0	0.25700633513555493	0.0
3	0.0	0.0	0.0	0.3606208397540788	0.0
4	0.0	0.0	0.0	0.5208967685336694	0.0
5	0.0	0.0	0.0	1.0020775855525725	0.0
6	0.0	0.0	0.0	1.4114166591647648	0.0
7	0.0	0.0	0.0	1.775214775040025	0.0
8	0.0	0.0	0.0	2.4456200366092817	0.0
9	0.0	0.0	0.0	2.7748211458316785	0.0
10	0.0	0.0	0.0	3.511419659925546	0.0
11	0.0	0.0	0.0	4.114925607609929	0.0
12	0.0	0.0	0.0	4.7032512360486685	0.0
13	0.0	0.0	0.0	4.9832045653927555	0.0
14	0.0	0.0	0.0	5.12353426074493	0.0
15	0.0	0.0	0.0	5.30887536781384	0.0
16	0.0	0.0	0.0	5.623425703810789	0.0
17	0.0	0.0	0.0	6.048298127348757	0.0
18	0.0	0.0	0.0	6.50688498083926	0.0
19	0.0	0.0	0.0	6.790015586304528	0.0
20	0.0	0.0	0.0	7.10244773822069	0.0
21	0.0	0.0	0.0	7.4528306882509625	0.0
22	0.0	0.0	0.0	7.841517467075477	0.0
23	0.0	0.0	0.0	8.238147436202944	0.0
24	0.0	0.0	0.0	8.537340937614184	0.0
25	0.0	0.0	0.0	8.813410929476825	0.0
26	0.0	0.0	0.0	9.066357411790872	0.0
27	0.0	0.0	0.0	9.344016041714106	0.0
28	0.0	0.0	0.0	9.638443628943573	0.0
29	0.0	0.0	0.0	9.941520467836257	0.0
30	0.0	0.0	0.0	10.282901135523183	0.0
31	0.0	0.0	0.0	10.60645375386348	0.0
32	0.0	0.0	0.0	10.92541697336207	0.0
33	0.0	0.0	0.0	11.242615039460004	0.0
34	0.0	0.0	0.0	11.540396418150719	0.0
35	0.0	0.0	0.0	11.881777085837644	0.0
36	0.0	0.0	0.0	12.210448649039845	0.0
37	0.0	0.0	0.0	12.556948261588673	0.0
38	0.0	0.0	0.0	12.878206180508117	0.0
39	0.0	0.0	0.0	13.237238382201605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	70	0.0	45.0	5
AGATACG	35	1.2104647E-7	45.0	1
CCGTGCC	25	3.888292E-5	45.0	12
CGTTCTA	40	6.8048394E-9	45.0	1
TTGACGA	20	7.0302555E-4	45.0	1
CGTGTAC	20	7.0302555E-4	45.0	18
ATGCGAA	25	3.888292E-5	45.0	1
CGCACGG	20	7.0302555E-4	45.0	2
TCGGTTA	20	7.0302555E-4	45.0	1
TGCGTAA	20	7.0302555E-4	45.0	1
CGTTATT	735	0.0	44.387756	1
CGTTTTA	1740	0.0	43.83621	1
CGTTTTT	3035	0.0	43.36903	1
TAGGCGA	105	0.0	42.857143	6
AGGCGAT	285	0.0	41.842106	7
CTCGTCC	135	0.0	41.666664	37
CCTCGTC	135	0.0	41.666664	36
TCCTCGT	140	0.0	40.17857	35
TCGTCCC	140	0.0	40.17857	38
CGGTTTT	325	0.0	40.153847	1
>>END_MODULE