##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288697
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07917643757988	33.0	31.0	34.0	30.0	34.0
2	32.27305444808918	33.0	31.0	34.0	30.0	34.0
3	32.11313245374909	33.0	31.0	34.0	30.0	34.0
4	35.858110752796186	37.0	35.0	37.0	35.0	37.0
5	32.07423353896992	37.0	35.0	37.0	0.0	37.0
6	33.998628319656945	37.0	35.0	37.0	19.0	37.0
7	20.71551141854609	32.0	0.0	37.0	0.0	37.0
8	28.081756997821245	35.0	17.0	37.0	17.0	37.0
9	34.91735625933072	37.0	32.0	39.0	32.0	39.0
10	36.32086928509822	37.0	35.0	39.0	32.0	39.0
11	36.7742373491931	39.0	37.0	39.0	33.0	39.0
12	37.13156700623838	39.0	37.0	39.0	34.0	39.0
13	36.9168851771927	39.0	37.0	39.0	33.0	39.0
14	38.37982382913574	40.0	38.0	41.0	34.0	41.0
15	38.51797559378864	40.0	38.0	41.0	34.0	41.0
16	38.599029432242105	40.0	38.0	41.0	34.0	41.0
17	38.57241675528322	40.0	38.0	41.0	34.0	41.0
18	38.47387052861651	40.0	38.0	41.0	34.0	41.0
19	38.39624935485994	40.0	37.0	41.0	34.0	41.0
20	38.3708143832461	40.0	37.0	41.0	34.0	41.0
21	38.333255974256744	40.0	37.0	41.0	34.0	41.0
22	38.292081317090236	40.0	37.0	41.0	34.0	41.0
23	38.274097063703465	40.0	37.0	41.0	34.0	41.0
24	38.15321946539105	40.0	37.0	41.0	34.0	41.0
25	38.027689930965686	40.0	36.0	41.0	33.0	41.0
26	37.88745293508419	40.0	36.0	41.0	33.0	41.0
27	37.88282870968524	40.0	36.0	41.0	33.0	41.0
28	37.80397440915562	40.0	36.0	41.0	33.0	41.0
29	37.797552451185844	40.0	36.0	41.0	33.0	41.0
30	37.68507466305504	40.0	36.0	41.0	33.0	41.0
31	37.568578821394055	40.0	36.0	41.0	33.0	41.0
32	37.45746924976706	40.0	36.0	41.0	33.0	41.0
33	37.30433638035726	40.0	36.0	41.0	32.0	41.0
34	37.169672701829256	40.0	36.0	41.0	32.0	41.0
35	37.05170126464771	40.0	36.0	41.0	31.0	41.0
36	36.907030554526024	40.0	35.0	41.0	31.0	41.0
37	36.82525970134778	40.0	35.0	41.0	31.0	41.0
38	36.7613761140573	40.0	35.0	41.0	31.0	41.0
39	36.70004884013343	40.0	35.0	41.0	31.0	41.0
40	36.548672137223456	39.0	35.0	41.0	30.0	41.0
41	36.43275475671725	39.0	35.0	41.0	30.0	41.0
42	36.3438345393267	39.0	35.0	41.0	30.0	41.0
43	36.274921457445004	39.0	35.0	41.0	30.0	41.0
44	36.17990834681344	39.0	35.0	41.0	29.0	41.0
45	36.12286930588125	39.0	35.0	40.0	29.0	41.0
46	36.03205783226012	39.0	35.0	40.0	29.0	41.0
47	35.98998950456707	39.0	35.0	40.0	28.0	41.0
48	35.93002698330776	39.0	35.0	40.0	28.0	41.0
49	35.78501681693956	39.0	35.0	40.0	28.0	41.0
50	35.68681351035861	39.0	35.0	40.0	27.0	41.0
51	33.84443205159735	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	13.0
10	20.0
11	5.0
12	11.0
13	3.0
14	10.0
15	12.0
16	20.0
17	54.0
18	61.0
19	132.0
20	227.0
21	424.0
22	687.0
23	1123.0
24	1708.0
25	2424.0
26	2983.0
27	3393.0
28	3665.0
29	4143.0
30	5088.0
31	6523.0
32	8804.0
33	12926.0
34	18603.0
35	24307.0
36	30335.0
37	54788.0
38	63370.0
39	42820.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.3204536243882	20.81351728628978	27.15407503368584	13.711954055636186
2	30.717326470313168	25.166870455875884	27.659795564207457	16.45600750960349
3	31.269116062861755	25.03732286792035	28.452668368566354	15.240892700651548
4	27.03491896348074	25.350453936133732	29.680599382743843	17.93402771764168
5	22.260016557151616	37.579538408781524	24.372958499741944	15.787486534324916
6	24.825335905811283	35.61519517002255	25.980526295735668	13.578942628430498
7	48.345150798241754	43.13692210171911	5.926628957003363	2.591298143035778
8	85.65277782588666	4.555294997869739	7.402917245416475	2.38900993082713
9	80.50551270016662	6.569171137905832	8.210684558551007	4.71463160337655
10	40.947775695625516	26.876621509748976	17.404060312369023	14.771542482256484
11	32.15135591987447	28.755061535104282	23.620266230684766	15.473316314336486
12	30.940744101947715	23.605718105834146	28.014492703422622	17.439045088795517
13	23.443956812852228	26.641080440738907	28.53164390346973	21.383318842939136
14	19.209759713471218	29.86349009515166	28.661191491425264	22.265558699951853
15	18.617789585620912	27.251062532689986	35.39039200268794	18.740755879001167
16	21.222596701732265	24.35598568741622	34.395230986120396	20.026186624731118
17	21.061874560525396	24.752595281558172	30.330069242146614	23.85546091576982
18	22.290151958627906	26.968759633802915	30.23342812706748	20.507660280501703
19	25.048753537445833	27.59363623452963	26.686803118840864	20.670807109183677
20	27.240671014939537	26.063312053814208	28.593300242122368	18.10271668912389
21	25.790707904827553	26.650779190639323	28.087926095525763	19.47058680900737
22	23.006473915558526	26.52192437053381	28.398978860189057	22.072622853718606
23	23.480327124978785	27.79384614318819	28.278783638208914	20.447043093624114
24	22.345227002705258	25.299189115231542	30.968454815948903	21.387129066114298
25	21.642414018850214	27.641090832256655	29.357076796780014	21.359418352113117
26	21.293605406360303	29.714198623470285	28.988870684489278	20.003325285680145
27	22.2877272711528	30.103534155186928	28.28640408455924	19.322334489101028
28	19.928159973951928	29.63834054389204	31.20676695635909	19.226732525796944
29	21.380201387614004	28.23652479935711	29.766156212222505	20.61711760080638
30	21.47649611876812	27.426332798747477	29.92168259455415	21.175488487930252
31	22.496943161861743	28.9005427836105	27.785186545062817	20.817327509464942
32	24.34905800891592	28.880106132034623	26.64489066391407	20.125945195135383
33	22.773703917948577	30.122931654987756	26.73495048441792	20.36841394264575
34	21.86548526655975	30.23342812706748	28.219205603106374	19.681881003266398
35	22.30366093170348	28.100049532901277	27.670187081957902	21.92610245343734
36	22.43320851965902	29.610629829890854	26.952133205402202	21.004028445047922
37	21.701645670027744	30.387568973699068	28.385816271038493	19.52496908523469
38	22.018586961416293	28.05363408694929	28.341132744711583	21.58664620692283
39	22.847137310051714	27.057433918606705	29.036325282216303	21.059103489125278
40	22.81804106035047	26.592933075161845	30.23273535921745	20.35629050527023
41	19.39091850625396	29.166219254096852	30.453381919451882	20.989480320197302
42	21.84400946320883	28.809097427406588	28.0654111403998	21.281481968984785
43	22.83362833697614	29.58569018728979	27.47413378039952	20.106547695334555
44	22.3438414670052	29.404531394507043	27.389962486620924	20.86166465186684
45	21.101362327977082	28.89569340866029	27.2139994527134	22.788944810649227
46	20.66803603778356	30.69100129201204	27.226815657938946	21.414147012265456
47	21.76399477653041	29.44679023335885	28.877335060634508	19.911879929476235
48	21.84816607030901	27.521934762051565	28.189070201630084	22.440828966009345
49	19.570345379411634	29.427392733558023	29.067499835467636	21.93476205156271
50	18.981146322961447	29.088282870968523	28.644565063024558	23.286005743045475
51	19.39507511335414	29.004111577189928	27.941405695244498	23.65940761421144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	186.0
1	409.5
2	633.0
3	2167.5
4	3702.0
5	2543.5
6	1385.0
7	1237.5
8	1090.0
9	1029.0
10	968.0
11	961.5
12	955.0
13	941.5
14	928.0
15	870.0
16	812.0
17	877.0
18	942.0
19	956.5
20	971.0
21	1061.5
22	1152.0
23	1441.0
24	1730.0
25	2271.5
26	3310.0
27	3807.0
28	4769.0
29	5731.0
30	6445.0
31	7159.0
32	8191.5
33	9224.0
34	10510.5
35	11797.0
36	12130.5
37	12464.0
38	13341.0
39	14218.0
40	15806.5
41	17395.0
42	18615.0
43	19835.0
44	20584.0
45	21333.0
46	21777.0
47	22221.0
48	20986.0
49	19751.0
50	18779.5
51	17808.0
52	16677.0
53	15546.0
54	14396.0
55	13246.0
56	13070.0
57	12894.0
58	12907.5
59	12921.0
60	12487.5
61	12054.0
62	10556.0
63	9058.0
64	7034.5
65	5011.0
66	3753.5
67	2496.0
68	2095.0
69	1694.0
70	1452.5
71	1211.0
72	942.0
73	673.0
74	521.5
75	319.0
76	268.0
77	195.0
78	122.0
79	91.5
80	61.0
81	45.0
82	29.0
83	22.0
84	15.0
85	13.0
86	11.0
87	6.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	288697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.303051295995452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.27172968771433	20.385386754971474
2	7.522518403365187	4.559105221044902
3	3.9927300992181425	3.629757150230172
4	2.9262493713136117	3.546971392151633
5	2.232408211787298	3.3824390277695993
6	1.942069406977276	3.5310377316009522
7	1.5831466325270906	3.3581921530185626
8	1.4334049654794019	3.474923535748553
9	1.304238489323762	3.5570165259770623
>10	9.724063828814412	47.71196098331469
>50	0.04115038178409767	0.8694236517871679
>100	0.02400438937405697	1.343276861207425
>500	0.0011430661606693796	0.24454705106045438
>1k	0.0011430661606693796	0.40596196011735486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.40596196011735486	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.24454705106045438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCG	467	0.1617612929819153	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGC	373	0.12920120403052335	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTC	299	0.10356879357942755	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015933660550681163	0.0
2	0.0	0.0	0.0	0.1056470971295164	0.0
3	0.0	0.0	0.0	0.1267765165554197	0.0
4	0.0	0.0	0.0	0.17388473035743357	0.0
5	0.0	0.0	0.0	0.402151736942192	0.0
6	0.0	0.0	0.0	0.46276892381978335	0.0
7	0.0	0.0	0.0	0.5777683869246996	0.0
8	0.0	0.0	0.0	0.8240473576102281	0.0
9	0.0	0.0	0.0	0.9040620442886487	0.0
10	0.0	0.0	0.0	1.4416498959116306	0.0
11	0.0	0.0	0.0	1.6650675275461817	0.0
12	0.0	0.0	0.0	2.11017087119021	0.0
13	0.0	0.0	0.0	2.2012698434691043	0.0
14	0.0	0.0	0.0	2.245260601945985	0.0
15	0.0	0.0	0.0	2.3301246635746127	0.0
16	0.0	0.0	0.0	2.481148054881069	0.0
17	0.0	0.0	0.0	2.6539936334634584	0.0
18	0.0	0.0	0.0	2.852818006421958	0.0
19	0.0	0.0	0.0	2.970242157001978	0.0
20	0.0	0.0	0.0	3.083163316556805	0.0
21	0.0	0.0	0.0	3.2314156364631432	0.0
22	0.0	0.0	0.0	3.3966407686952063	0.0
23	0.0	0.0	0.0	3.556323758127033	0.0
24	0.0	0.0	0.0	3.6993803191581485	0.0
25	0.0	0.0	0.0	3.827195987488613	0.0
26	0.0	0.0	0.0	3.932843084618129	0.0
27	0.0	0.0	0.0	4.055116610148357	0.0
28	0.0	0.0	0.0	4.186049733803953	0.0
29	0.0	0.0	0.0	4.322525000259788	0.0
30	0.0	0.0	0.0	4.48255437361663	0.0
31	0.0	0.0	0.0	4.628382006047864	0.0
32	0.0	0.0	0.0	4.772131334929009	0.0
33	0.0	0.0	0.0	4.915534279885139	0.0
34	0.0	0.0	0.0	5.068982358666699	0.0
35	0.0	0.0	0.0	5.245291776499236	0.0
36	0.0	0.0	0.0	5.414327131906463	0.0
37	0.0	0.0	0.0	5.582669719463659	0.0
38	0.0	0.0	0.0	5.750319539170826	0.0
39	0.0	0.0	0.0	5.929400028403482	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.024498E-4	45.000004	1
GATGTCG	20	7.024498E-4	45.000004	9
CGGCACG	20	7.024498E-4	45.000004	2
ATAGCCG	20	7.024498E-4	45.000004	45
TATGCGG	20	7.024498E-4	45.000004	2
TTATCCG	20	7.024498E-4	45.000004	10
TCTACGG	20	7.024498E-4	45.000004	2
CAGGCGA	20	7.024498E-4	45.000004	6
GTACTAT	20	7.024498E-4	45.000004	9
TTGCGAA	20	7.024498E-4	45.000004	1
CAATCGG	20	7.024498E-4	45.000004	2
ATGGGCG	25	3.883525E-5	45.0	5
CGAATAT	35	1.2076998E-7	45.0	14
AATCGAG	25	3.883525E-5	45.0	1
TACCGGG	25	3.883525E-5	45.0	3
TAGGCAC	65	0.0	44.999996	6
CGTTATT	95	0.0	42.63158	1
CGTTTTA	285	0.0	42.63158	1
TACGGCT	80	0.0	42.187504	7
AAACGGC	60	3.6379788E-12	41.250004	6
>>END_MODULE