##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275435
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88364586926135	31.0	31.0	34.0	30.0	34.0
2	32.07712527456569	33.0	31.0	34.0	30.0	34.0
3	31.879452502405286	33.0	31.0	34.0	30.0	34.0
4	35.68808974894258	37.0	35.0	37.0	35.0	37.0
5	31.984725978906095	37.0	35.0	37.0	0.0	37.0
6	33.8924464937281	37.0	35.0	37.0	19.0	37.0
7	20.85157659701926	32.0	0.0	37.0	0.0	37.0
8	28.148594042151505	35.0	17.0	37.0	17.0	37.0
9	34.81638136039356	37.0	32.0	39.0	32.0	39.0
10	36.23366311470946	37.0	35.0	39.0	32.0	39.0
11	36.61316099987293	38.0	35.0	39.0	32.0	39.0
12	36.97141612358633	39.0	37.0	39.0	33.0	39.0
13	36.74446239584657	39.0	37.0	39.0	33.0	39.0
14	38.110280102383506	40.0	37.0	41.0	33.0	41.0
15	38.32238459164594	40.0	38.0	41.0	34.0	41.0
16	38.39578121879936	40.0	38.0	41.0	34.0	41.0
17	38.355655599324706	40.0	37.0	41.0	34.0	41.0
18	38.26258463884401	40.0	37.0	41.0	34.0	41.0
19	38.20215295804818	40.0	37.0	41.0	34.0	41.0
20	38.14017826347414	40.0	37.0	41.0	34.0	41.0
21	38.06249024270699	40.0	37.0	41.0	34.0	41.0
22	38.00472706809229	40.0	37.0	41.0	34.0	41.0
23	37.937095866538385	40.0	36.0	41.0	34.0	41.0
24	37.83143754424819	40.0	36.0	41.0	33.0	41.0
25	37.697478533955376	40.0	36.0	41.0	33.0	41.0
26	37.53797810735745	39.0	36.0	41.0	33.0	41.0
27	37.45900847749923	39.0	35.0	41.0	33.0	41.0
28	37.34391780274838	39.0	35.0	41.0	33.0	41.0
29	37.318641421751046	39.0	35.0	41.0	33.0	41.0
30	37.19179116669995	39.0	35.0	41.0	32.0	41.0
31	37.05499301105524	39.0	35.0	41.0	32.0	41.0
32	36.94376894730154	39.0	35.0	41.0	31.0	41.0
33	36.753328371485104	39.0	35.0	41.0	31.0	41.0
34	36.57988999219417	39.0	35.0	41.0	30.0	41.0
35	36.40641530669668	39.0	35.0	41.0	30.0	41.0
36	36.24236571241854	39.0	35.0	41.0	30.0	41.0
37	36.14753753154102	39.0	35.0	41.0	29.0	41.0
38	36.02156951730898	39.0	35.0	40.0	29.0	41.0
39	35.951342421987036	39.0	35.0	40.0	28.0	41.0
40	35.87637373609019	39.0	35.0	40.0	28.0	41.0
41	35.775591337339115	39.0	35.0	40.0	27.0	41.0
42	35.67490696534573	39.0	35.0	40.0	27.0	41.0
43	35.57748652132082	39.0	35.0	40.0	26.0	41.0
44	35.433223809610254	38.0	35.0	40.0	26.0	41.0
45	35.33109081997568	38.0	34.0	40.0	26.0	41.0
46	35.19351208089023	38.0	34.0	40.0	25.0	41.0
47	35.15389837892788	38.0	34.0	40.0	25.0	41.0
48	35.04445331929493	38.0	34.0	40.0	24.0	41.0
49	34.90319676148638	38.0	34.0	40.0	24.0	41.0
50	34.74774810753898	38.0	34.0	40.0	24.0	41.0
51	32.890990614845606	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	14.0
11	9.0
12	6.0
13	11.0
14	12.0
15	23.0
16	23.0
17	52.0
18	96.0
19	207.0
20	360.0
21	653.0
22	967.0
23	1462.0
24	2170.0
25	2948.0
26	3660.0
27	4038.0
28	4218.0
29	4710.0
30	5545.0
31	7431.0
32	9348.0
33	13891.0
34	19375.0
35	25139.0
36	31138.0
37	51332.0
38	52858.0
39	33712.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.862889610978996	20.04756113057527	26.42510937244722	14.664439885998512
2	31.97923284985568	24.25762884165048	27.460925445204857	16.30221286328898
3	31.828199030624287	22.80538057981012	29.230126890191876	16.136293499373718
4	27.220578357870277	26.26862962223392	28.32356091273803	18.18723110715777
5	21.510338192313977	37.11220433133044	24.66389529290032	16.71356218345526
6	26.193112712618223	33.451812587361815	26.958447546608095	13.396627153411874
7	48.64015103381923	42.56939023726106	6.340878973260479	2.44957975565923
8	83.26392796848621	4.877012725325394	9.021003140486867	2.8380561657015266
9	77.26941020567466	7.9216512062737126	10.815618930056093	3.9933196579955346
10	39.96441991758491	31.033456169332148	17.462559224499426	11.539564688583512
11	29.467932543068237	24.962332310708515	28.76903806705756	16.800697079165683
12	27.442772341931853	23.53077858659938	31.057781327717976	17.968667743750792
13	23.225806451612904	25.801731806052246	31.825657596166064	19.14680414616879
14	18.75360792927551	29.541997204422092	32.277306805598414	19.42708806070398
15	19.103236698313577	26.643309673788735	35.986711928404155	18.266741699493526
16	21.8385462994899	24.466752591355494	34.24437707626119	19.450324032893423
17	21.84471835460272	23.29478824405032	32.32414181204277	22.53635158930419
18	23.20656416214352	24.390146495543412	32.31107157768621	20.092217764626863
19	24.81747054658994	25.458275092126996	29.42291284695119	20.301341514331874
20	25.50619928476773	26.45197596529127	29.925390745547954	18.11643400439305
21	24.219144262711712	25.973823225080327	30.0513732822626	19.755659229945362
22	23.455261676983678	24.447873363951565	30.19768729464302	21.89917766442173
23	21.8458075407991	25.537422622397298	31.319186014849237	21.297583821954362
24	21.84980122351916	24.51068310127616	32.68793000163378	20.951585673570897
25	21.251474939640932	26.288961097899687	30.865721495089588	21.593842467369797
26	20.44112040953401	28.684081543739904	29.406575054005486	21.468222992720605
27	21.188302140250876	28.31412129903607	30.350173362136257	20.147403198576797
28	19.946266814311905	27.07898415234084	32.73839562873273	20.23635340461452
29	20.15793199847514	25.909198177428433	32.09976945558844	21.833100368507996
30	20.972643273367584	26.450160654963966	31.685878700963933	20.89131737070452
31	22.941891916423113	26.976963711946556	28.70622832973297	21.374916041897364
32	23.442917566758037	27.891517054840527	28.501461324813476	20.164104053587963
33	23.030842122460836	27.324051046526403	28.420861546281333	21.224245284731424
34	20.904387605061086	27.39012834244014	29.51513061157805	22.190353440920727
35	21.994662987637735	27.101130938333906	28.9861491822027	21.918056891825657
36	22.478987782961497	27.935810626826658	28.686622978198123	20.898578612013726
37	21.76012489335052	28.0400094396137	29.93192586272623	20.267939804309545
38	22.272768529780166	28.38891208452085	27.515021692958413	21.823297692740574
39	21.729990741917334	27.726686877121644	28.57262148964366	21.97070089131737
40	22.235010074972315	26.955906112149876	29.057309347032877	21.751774465844935
41	20.306424383248316	27.297910577813276	28.569716993119975	23.825948045818432
42	21.390527710712146	26.979868208470236	27.935810626826658	23.69379345399096
43	21.671174687312796	26.168061430101474	28.497104580027955	23.66365930255777
44	20.991885562836966	27.50776045164921	28.27382140977	23.226532575743825
45	20.89204349483544	29.374625592244996	27.652622215767785	22.080708697151778
46	22.58064516129032	28.205202679398045	26.449434530833045	22.76471762847859
47	21.108065423784193	28.35514731243306	28.677546426561623	21.859240837221122
48	21.386534027992084	27.446039900520997	29.488264018734	21.67916205275292
49	21.32771797338755	25.653602483344528	30.06226514422641	22.956414399041517
50	20.455279830086955	27.090602138435564	29.38297601975058	23.071142011726906
51	19.920489407664242	27.389402218309222	28.74071922595168	23.94938914807486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	192.0
1	468.0
2	744.0
3	2480.5
4	4217.0
5	2874.0
6	1531.0
7	1396.0
8	1261.0
9	1209.0
10	1157.0
11	1142.0
12	1127.0
13	1152.0
14	1177.0
15	1146.0
16	1115.0
17	1123.5
18	1132.0
19	1201.0
20	1270.0
21	1308.5
22	1347.0
23	1553.5
24	1760.0
25	2263.5
26	3318.0
27	3869.0
28	4363.5
29	4858.0
30	5431.0
31	6004.0
32	6732.5
33	7461.0
34	8460.0
35	9459.0
36	10290.0
37	11121.0
38	12096.0
39	13071.0
40	14484.5
41	15898.0
42	17246.0
43	18594.0
44	19341.5
45	20089.0
46	20605.5
47	21122.0
48	20755.5
49	20389.0
50	19715.5
51	19042.0
52	17372.0
53	15702.0
54	14529.5
55	13357.0
56	12385.0
57	11413.0
58	10931.5
59	10450.0
60	9773.0
61	9096.0
62	8098.0
63	7100.0
64	6136.0
65	5172.0
66	4408.0
67	3644.0
68	3133.0
69	2622.0
70	2377.0
71	2132.0
72	1754.0
73	1376.0
74	1008.5
75	510.5
76	380.0
77	332.0
78	284.0
79	213.0
80	142.0
81	109.0
82	76.0
83	49.0
84	22.0
85	21.0
86	20.0
87	18.0
88	16.0
89	13.5
90	11.0
91	6.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	275435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.934636798673495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28993879420699	25.961333599650256
2	8.382822279628664	6.192329924918258
3	4.260597982050438	4.720909170365825
4	2.8372961228075457	4.191780071447249
5	2.159446169451968	3.987917997749759
6	1.7245569156011225	3.821752998982085
7	1.411637277698435	3.649681710028193
8	1.2146878824838498	3.589124461063263
9	1.0277359202474063	3.4163147645359575
>10	6.647291958848188	38.44757265739949
>50	0.032991522731535966	0.7791206391201861
>100	0.008997688017691628	0.4674723834721116
>500	9.997431130768476E-4	0.2806311306783609
>1k	9.997431130768476E-4	0.49405849058900897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1338	0.4857770435856009	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	760	0.2759271697496687	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.10565106104888632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.630620654600904E-4	0.0	0.0	0.03921070306968976	0.0
2	3.630620654600904E-4	0.0	0.0	0.18661390164648647	0.0
3	3.630620654600904E-4	0.0	0.0	0.24325158385826057	0.0
4	3.630620654600904E-4	0.0	0.0	0.3125964383611378	0.0
5	3.630620654600904E-4	0.0	0.0	0.5496759671065768	0.0
6	3.630620654600904E-4	0.0	0.0	0.7105124621053969	0.0
7	3.630620654600904E-4	0.0	0.0	0.895674115490043	0.0
8	3.630620654600904E-4	0.0	0.0	1.3549476282970574	0.0
9	3.630620654600904E-4	0.0	0.0	1.529943543848821	0.0
10	3.630620654600904E-4	0.0	0.0	1.9362099950986622	0.0
11	3.630620654600904E-4	0.0	0.0	2.4499428177246902	0.0
12	3.630620654600904E-4	0.0	0.0	2.9248279993464883	0.0
13	3.630620654600904E-4	0.0	0.0	3.124149073284078	0.0
14	3.630620654600904E-4	0.0	0.0	3.2152776517145605	0.0
15	3.630620654600904E-4	0.0	0.0	3.3630439123568174	0.0
16	3.630620654600904E-4	0.0	0.0	3.6858060885508377	0.0
17	3.630620654600904E-4	0.0	0.0	4.093161725997059	0.0
18	3.630620654600904E-4	0.0	0.0	4.590556755677383	0.0
19	3.630620654600904E-4	0.0	0.0	4.857770435856009	0.0
20	3.630620654600904E-4	0.0	0.0	5.1046526403688715	0.0
21	3.630620654600904E-4	0.0	0.0	5.403452720242526	0.0
22	3.630620654600904E-4	0.0	0.0	5.7483616824296115	0.0
23	3.630620654600904E-4	0.0	0.0	6.087098589503876	0.0
24	3.630620654600904E-4	0.0	0.0	6.374280683282807	0.0
25	3.630620654600904E-4	0.0	0.0	6.6454880461814945	0.0
26	3.630620654600904E-4	0.0	0.0	6.862962223392089	0.0
27	3.630620654600904E-4	0.0	0.0	7.105850745184889	0.0
28	3.630620654600904E-4	0.0	0.0	7.354185197959591	0.0
29	3.630620654600904E-4	0.0	0.0	7.614137636829016	0.0
30	3.630620654600904E-4	0.0	0.0	7.936173688892116	0.0
31	3.630620654600904E-4	0.0	0.0	8.212826982772706	0.0
32	3.630620654600904E-4	0.0	0.0	8.492021711111514	0.0
33	3.630620654600904E-4	0.0	0.0	8.766496632599344	0.0
34	3.630620654600904E-4	0.0	0.0	9.03988236789079	0.0
35	3.630620654600904E-4	0.0	0.0	9.35647248897199	0.0
36	3.630620654600904E-4	0.0	0.0	9.64147621035816	0.0
37	3.630620654600904E-4	0.0	0.0	9.930110552398933	0.0
38	3.630620654600904E-4	0.0	0.0	10.209668342803202	0.0
39	3.630620654600904E-4	0.0	0.0	10.496850436582134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTC	20	7.0239377E-4	45.0	27
GTCGCCT	20	7.0239377E-4	45.0	34
ATAGGTA	20	7.0239377E-4	45.0	5
ACCGCTC	20	7.0239377E-4	45.0	18
ACCGCAA	20	7.0239377E-4	45.0	31
GGCCGAT	25	3.883057E-5	45.0	8
TTCGTAA	20	7.0239377E-4	45.0	1
TTACTAT	20	7.0239377E-4	45.0	13
CGACAAG	20	7.0239377E-4	45.0	2
GCGTTCC	20	7.0239377E-4	45.0	28
CGTTCCG	20	7.0239377E-4	45.0	29
GTCAGAC	20	7.0239377E-4	45.0	32
GTTACGG	20	7.0239377E-4	45.0	2
GCTCTAC	20	7.0239377E-4	45.0	22
ACGATGG	25	3.883057E-5	45.0	2
CGGATAG	20	7.0239377E-4	45.0	2
TACTGCG	20	7.0239377E-4	45.0	27
AGACCGC	20	7.0239377E-4	45.0	29
CGAATAT	25	3.883057E-5	45.0	14
TCGTAAG	25	3.883057E-5	45.0	2
>>END_MODULE