FastQCFastQC Report
Sat 18 Jun 2016
SRR3551095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463909
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33650.7253577749084411No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20060.4324123912232787No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.15800512600531572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG302.162691E-645.0000041
TATCACG207.0289325E-445.01
AGGACCG453.8380676E-1045.07
TGTTGCG207.0289325E-445.01
TACCTAC207.0289325E-445.032
CGCACGT207.0289325E-445.027
CACGTTG207.0289325E-445.029
CGGTAAG207.0289325E-445.02
CGTTTTA8600.044.2151151
CGTTATT3400.043.014711
AGGCGAT2000.042.757
GCTACGA650.041.5384610
ATAGCGG900.040.02
TCTACGG451.924127E-840.02
GAACGTC403.4522782E-739.37533
CGTTTTT17750.039.169011
CCGATAA356.240236E-638.57142611
CTACGAA700.038.57142611
TCGACAC356.240236E-638.57142634
ATCGAAG356.240236E-638.5714262