##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463909
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861500854693485	31.0	31.0	34.0	30.0	34.0
2	32.04766452041241	33.0	31.0	34.0	30.0	34.0
3	31.69722941352722	33.0	31.0	34.0	30.0	34.0
4	35.59829837317233	37.0	35.0	37.0	33.0	37.0
5	31.99832941374278	37.0	35.0	37.0	0.0	37.0
6	33.90184928509686	37.0	35.0	37.0	19.0	37.0
7	20.881897096197747	32.0	0.0	37.0	0.0	37.0
8	28.164133483075343	35.0	17.0	37.0	17.0	37.0
9	34.83535995205956	37.0	32.0	39.0	32.0	39.0
10	36.22991793649185	37.0	35.0	39.0	32.0	39.0
11	36.63635325031418	38.0	35.0	39.0	32.0	39.0
12	37.06945543199205	39.0	37.0	39.0	34.0	39.0
13	36.933698203742544	39.0	37.0	39.0	33.0	39.0
14	38.386879754434595	40.0	38.0	41.0	34.0	41.0
15	38.52835146548138	40.0	38.0	41.0	34.0	41.0
16	38.563651491995195	40.0	38.0	41.0	34.0	41.0
17	38.51962561623077	40.0	38.0	41.0	34.0	41.0
18	38.39265674949182	40.0	38.0	41.0	34.0	41.0
19	38.29586405954616	40.0	37.0	41.0	34.0	41.0
20	38.20796751086959	40.0	37.0	41.0	34.0	41.0
21	38.13598572133759	40.0	37.0	41.0	34.0	41.0
22	38.09292986339993	40.0	37.0	41.0	34.0	41.0
23	38.0066435443158	40.0	36.0	41.0	34.0	41.0
24	37.858032502063985	40.0	36.0	41.0	33.0	41.0
25	37.710395788829274	40.0	36.0	41.0	33.0	41.0
26	37.570170011791106	39.0	36.0	41.0	33.0	41.0
27	37.53075926528694	39.0	36.0	41.0	33.0	41.0
28	37.397769821236494	39.0	35.0	41.0	33.0	41.0
29	37.36778333681821	39.0	35.0	41.0	33.0	41.0
30	37.21391264235012	39.0	35.0	41.0	32.0	41.0
31	37.079021963359196	39.0	35.0	41.0	32.0	41.0
32	36.913586500800804	39.0	35.0	41.0	31.0	41.0
33	36.62060878318808	39.0	35.0	41.0	30.0	41.0
34	36.38626972100132	39.0	35.0	41.0	30.0	41.0
35	36.15858929229655	39.0	35.0	41.0	29.0	41.0
36	35.92393766881005	39.0	35.0	41.0	27.0	41.0
37	35.79400054752117	39.0	35.0	41.0	26.0	41.0
38	35.65274870718179	39.0	35.0	41.0	25.0	41.0
39	35.568889588259765	39.0	35.0	41.0	25.0	41.0
40	35.4472967758763	39.0	35.0	40.0	24.0	41.0
41	35.340187407444134	39.0	35.0	40.0	24.0	41.0
42	35.230911665865506	39.0	35.0	40.0	23.0	41.0
43	35.13297651047942	39.0	35.0	40.0	23.0	41.0
44	34.984158531091225	39.0	34.0	40.0	23.0	41.0
45	34.91349596580364	38.0	34.0	40.0	23.0	41.0
46	34.76093156200893	38.0	34.0	40.0	22.0	41.0
47	34.76162135246352	38.0	34.0	40.0	22.0	41.0
48	34.65745868262957	38.0	34.0	40.0	22.0	41.0
49	34.4779493392023	38.0	34.0	40.0	20.0	41.0
50	34.32313018285914	38.0	34.0	40.0	20.0	41.0
51	32.51104419185659	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	26.0
10	31.0
11	12.0
12	8.0
13	6.0
14	9.0
15	16.0
16	43.0
17	78.0
18	171.0
19	350.0
20	599.0
21	1121.0
22	1905.0
23	2996.0
24	4667.0
25	6863.0
26	7949.0
27	8306.0
28	8144.0
29	8219.0
30	9515.0
31	11688.0
32	15326.0
33	21132.0
34	29507.0
35	38098.0
36	50263.0
37	85405.0
38	91226.0
39	60215.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.695558827270006	21.07697845913746	25.427400632451626	16.80006208114091
2	34.86524296790965	24.2963598464354	25.932887699958396	14.905509485696546
3	30.42514803549834	23.154541084566155	31.064713122616723	15.355597757318783
4	27.038276903444427	24.923638041081333	30.77068994134626	17.267395114127986
5	21.591303466843716	36.11656596444561	26.29696772427351	15.995162844437164
6	25.035513430435714	33.97864667423999	27.947075827371314	13.038764067952982
7	46.983567898014485	42.691993472857824	7.814894731509843	2.509543897617852
8	81.6461849198873	4.572664035403495	11.172234209726478	2.6089168349827228
9	75.16474136091345	7.69547475905835	12.922361928740333	4.217421951287861
10	38.09842016429946	30.440021642175513	19.79396821359361	11.667589979931408
11	27.295654966814613	26.01803370919728	30.388071798563942	16.29823952542417
12	25.661282708462217	23.0915977055845	32.983408383971856	18.263711201981423
13	21.24080369210341	24.743645844335852	34.31535063988843	19.700199823672314
14	19.084346283430587	27.865163210888344	33.452465893095415	19.598024612585657
15	19.03756986822847	26.37672474558588	37.065674518062806	17.520030868122845
16	22.22009057810907	24.64750629972689	35.48368322235611	17.648719899807936
17	21.455500971095624	24.364907772860626	33.21362594819243	20.965965307851324
18	22.378957942182627	25.75483553886646	32.63441752585097	19.23178899309994
19	23.907274918141276	26.31873923549662	31.31799555516276	18.45599029119935
20	24.80033799732275	25.3053939457954	31.65663955646474	18.23762850041711
21	23.544919369962646	24.77059078396841	32.785093628276236	18.899396217792713
22	22.50312022400945	24.874921590225668	31.6857400912679	20.93621809449698
23	21.94676111047641	25.823383465291684	32.379195057651394	19.850660366580513
24	21.072451709279193	25.191578520787484	33.718250777630956	20.01771899230237
25	21.605314835452642	26.43363245808984	31.571277987708797	20.389774718748722
26	20.566749082255356	28.281193078815026	31.095107014522245	20.056950824407373
27	21.06792495942092	27.323893263549532	32.024168533052816	19.584013243976727
28	20.09445818037589	28.239374532505295	32.94137427814507	18.72479300897374
29	20.669570971893194	26.451955017039978	33.11252853469107	19.765945476375755
30	20.801062277300076	25.593597020105236	33.143569105147776	20.461771597446912
31	21.311291654182178	26.916054657271143	31.22724499848031	20.54540869006637
32	22.262771362487037	26.71256647316607	31.372963232013173	19.651698932333712
33	21.75017083091727	27.40515920148133	30.951113257125858	19.893556710475547
34	21.169022372922274	26.98395590514519	32.22744115764083	19.619580564291702
35	21.33478764154177	26.15060281218946	31.000476386532704	21.51413315973607
36	21.44299851910612	27.906335078646887	30.58854214943017	20.062124252816822
37	21.90558924271786	28.26631947213785	30.712057752705814	19.116033532438472
38	22.039020583778285	27.132907531433965	30.027871845555914	20.800200039231832
39	22.810508095337664	26.135082526961106	30.465242105671585	20.589167272029645
40	22.247251077258685	26.10447307553852	31.665477496664217	19.982798350538573
41	19.90476580536269	27.67611751442632	31.3477427685171	21.07137391169389
42	21.818072078791314	27.299319478604637	29.858657624663458	21.023950817940587
43	22.49859347415118	26.976411322048072	30.35961794231196	20.165377261488786
44	21.345996736428912	27.998594551948763	30.065163642007377	20.590245069614944
45	21.21407431198791	27.291990455024585	29.576921335865443	21.917013897122064
46	20.18693321319483	28.52693092826395	29.76661371087864	21.51952214766258
47	21.446231911862025	27.042803653302695	31.00823652914688	20.5027279056884
48	21.653815726791244	25.63692448303439	31.116447406711227	21.592812383463137
49	20.87844814392478	27.403650284861904	30.46157759388156	21.25632397733176
50	19.92438172141519	27.74100092906152	30.183936935907692	22.1506804136156
51	20.266474674990135	27.174510518226636	29.788600781618808	22.770414025164417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	674.5
2	1145.0
3	5715.0
4	10285.0
5	7266.5
6	4248.0
7	3746.5
8	3245.0
9	3096.0
10	2947.0
11	2893.5
12	2840.0
13	2730.0
14	2620.0
15	2565.5
16	2511.0
17	2594.0
18	2677.0
19	2817.0
20	2957.0
21	2979.0
22	3001.0
23	3293.0
24	3585.0
25	4267.5
26	5856.0
27	6762.0
28	8107.0
29	9452.0
30	10537.0
31	11622.0
32	12893.5
33	14165.0
34	15521.5
35	16878.0
36	18154.5
37	19431.0
38	21020.5
39	22610.0
40	24460.5
41	26311.0
42	27798.0
43	29285.0
44	31147.5
45	33010.0
46	33629.0
47	34248.0
48	33100.5
49	31953.0
50	30309.5
51	28666.0
52	26294.0
53	23922.0
54	22170.5
55	20419.0
56	19530.5
57	18642.0
58	18534.0
59	18426.0
60	17603.5
61	16781.0
62	14614.5
63	12448.0
64	10025.5
65	7603.0
66	6062.5
67	4522.0
68	3839.0
69	3156.0
70	2719.0
71	2282.0
72	1930.0
73	1578.0
74	1298.0
75	830.5
76	643.0
77	516.0
78	389.0
79	329.5
80	270.0
81	181.0
82	92.0
83	78.5
84	65.0
85	44.0
86	23.0
87	18.0
88	13.0
89	9.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.71657252859063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15348284987883	29.785590901761022
2	7.9466700268130905	6.4712233301502495
3	3.922145339708105	4.790889455756965
4	2.675398866918733	4.357322880312232
5	1.940802095897615	3.9511404650627977
6	1.554440261517322	3.7974887789658864
7	1.349515135153988	3.8463341615249695
8	1.0624358485661785	3.460699702809563
9	0.9523727682293373	3.4899619402678
>10	5.397767200897567	33.1248090754848
>50	0.03088274175780397	0.874251284394887
>100	0.012461457200516982	0.7037248542063134
>500	5.418024869789992E-4	0.16170229408565132
>1k	0.0010836049739579984	1.184860875216962
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3365	0.7253577749084411	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2006	0.4324123912232787	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.15800512600531572	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0379384750026406	0.0
2	0.0	0.0	0.0	0.14399375739638592	0.0
3	0.0	0.0	0.0	0.20133258893446776	0.0
4	0.0	0.0	0.0	0.26643156308672605	0.0
5	0.0	0.0	0.0	0.469273068640617	0.0
6	0.0	0.0	0.0	0.6512053010396436	0.0
7	0.0	0.0	0.0	0.8059770342890524	0.0
8	0.0	0.0	0.0	1.1472077497957573	0.0
9	0.0	0.0	0.0	1.3034883996645894	0.0
10	0.0	0.0	0.0	1.6779152807986049	0.0
11	0.0	0.0	0.0	2.065922411507429	0.0
12	0.0	0.0	0.0	2.412326555423585	0.0
13	0.0	0.0	0.0	2.5903787165155236	0.0
14	0.0	0.0	0.0	2.682207070783279	0.0
15	0.0	0.0	0.0	2.7936513411035353	0.0
16	0.0	0.0	0.0	3.0374491548989133	0.0
17	0.0	0.0	0.0	3.3456992642953685	0.0
18	0.0	0.0	0.0	3.702665824547487	0.0
19	0.0	0.0	0.0	3.903782853964894	0.0
20	0.0	0.0	0.0	4.122144644747138	0.0
21	0.0	0.0	0.0	4.381462743770869	0.0
22	0.0	0.0	0.0	4.66427683015419	0.0
23	0.0	0.0	0.0	4.974035856170068	0.0
24	0.0	0.0	0.0	5.19153540888407	0.0
25	0.0	0.0	0.0	5.402999295120379	0.0
26	0.0	0.0	0.0	5.603038526952484	0.0
27	0.0	0.0	0.0	5.79855100892632	0.0
28	0.0	0.0	0.0	6.0061348238555405	0.0
29	0.0	0.0	0.0	6.236783507110231	0.0
30	0.0	0.0	0.0	6.4842458326956365	0.0
31	0.0	0.0	0.0	6.750461836265302	0.0
32	0.0	0.0	0.0	7.002666471226038	0.0
33	0.0	0.0	0.0	7.255517784737955	0.0
34	0.0	0.0	0.0	7.4911243368850355	0.0
35	0.0	0.0	0.0	7.742897852811651	0.0
36	0.0	0.0	0.0	7.9966114043918095	0.0
37	0.0	0.0	0.0	8.23739138494834	0.0
38	0.0	0.0	0.0	8.487655984255532	0.0
39	0.0	0.0	0.0	8.750854154586351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	2.162691E-6	45.000004	1
TATCACG	20	7.0289325E-4	45.0	1
AGGACCG	45	3.8380676E-10	45.0	7
TGTTGCG	20	7.0289325E-4	45.0	1
TACCTAC	20	7.0289325E-4	45.0	32
CGCACGT	20	7.0289325E-4	45.0	27
CACGTTG	20	7.0289325E-4	45.0	29
CGGTAAG	20	7.0289325E-4	45.0	2
CGTTTTA	860	0.0	44.215115	1
CGTTATT	340	0.0	43.01471	1
AGGCGAT	200	0.0	42.75	7
GCTACGA	65	0.0	41.53846	10
ATAGCGG	90	0.0	40.0	2
TCTACGG	45	1.924127E-8	40.0	2
GAACGTC	40	3.4522782E-7	39.375	33
CGTTTTT	1775	0.0	39.16901	1
CCGATAA	35	6.240236E-6	38.571426	11
CTACGAA	70	0.0	38.571426	11
TCGACAC	35	6.240236E-6	38.571426	34
ATCGAAG	35	6.240236E-6	38.571426	2
>>END_MODULE