FastQCFastQC Report
Sat 18 Jun 2016
SRR3551093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157245
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16221.0315113358135393No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.5456453305351522No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.20286813571178736No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2400.15262806448535726No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1660.10556774460237209No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.10302394352761614No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1590.10111609272154917No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAA207.014698E-445.0000041
CGGGTAC207.014698E-445.0000046
AGGTAAT253.875405E-545.0000047
TTAATCG207.014698E-445.00000420
CGAAAAG207.014698E-445.0000042
TTAGCGG253.875405E-545.0000042
ATAATGC207.014698E-445.00000432
GACAAAC207.014698E-445.00000412
CCTAATA207.014698E-445.00000428
TACGGGA207.014698E-445.0000044
ACGGGTC207.014698E-445.0000045
ACGGGTA207.014698E-445.0000045
ACGGGCG207.014698E-445.0000045
TACGAAA207.014698E-445.00000420
GAGCGAT207.014698E-445.0000046
GTGTATT207.014698E-445.0000049
GCGATTG253.875405E-545.0000049
CATACGA207.014698E-445.00000418
CGATGCC207.014698E-445.00000418
GGTTAGT207.014698E-445.00000438