##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157245
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879372953035073	31.0	31.0	34.0	30.0	34.0
2	32.07118827307705	33.0	31.0	34.0	30.0	34.0
3	31.666730261693537	33.0	31.0	34.0	30.0	34.0
4	35.6002416611021	37.0	35.0	37.0	33.0	37.0
5	32.04044643708862	37.0	35.0	37.0	10.0	37.0
6	33.94015072021368	37.0	35.0	37.0	19.0	37.0
7	20.720550732932686	32.0	0.0	37.0	0.0	37.0
8	28.097077808515373	35.0	17.0	37.0	17.0	37.0
9	34.833482781646474	37.0	32.0	39.0	32.0	39.0
10	36.278813316798626	37.0	35.0	39.0	32.0	39.0
11	36.70921809914465	38.0	35.0	39.0	33.0	39.0
12	37.02330757734745	39.0	37.0	39.0	34.0	39.0
13	36.74906038347801	39.0	35.0	39.0	33.0	39.0
14	38.117326465070434	40.0	37.0	41.0	33.0	41.0
15	38.350605742630925	40.0	38.0	41.0	34.0	41.0
16	38.425164552132024	40.0	38.0	41.0	34.0	41.0
17	38.41738688034596	40.0	37.0	41.0	34.0	41.0
18	38.276886387484495	40.0	37.0	41.0	34.0	41.0
19	38.16059652135203	40.0	37.0	41.0	34.0	41.0
20	38.06276193201692	40.0	36.0	41.0	34.0	41.0
21	37.94219848007886	40.0	36.0	41.0	34.0	41.0
22	37.83807434258641	39.0	36.0	41.0	33.0	41.0
23	37.775248815542625	39.0	35.0	41.0	33.0	41.0
24	37.614957550319566	39.0	35.0	41.0	33.0	41.0
25	37.47193233489141	39.0	35.0	41.0	33.0	41.0
26	37.30723393430634	39.0	35.0	41.0	33.0	41.0
27	37.2947375115266	39.0	35.0	41.0	33.0	41.0
28	37.187471779706826	39.0	35.0	41.0	33.0	41.0
29	37.148379916690516	39.0	35.0	41.0	33.0	41.0
30	37.00648033323794	39.0	35.0	41.0	32.0	41.0
31	36.80883334923209	39.0	35.0	41.0	31.0	41.0
32	36.60519571369519	39.0	35.0	41.0	31.0	41.0
33	36.34257369073738	39.0	35.0	41.0	30.0	41.0
34	36.058780883334926	39.0	35.0	41.0	29.0	41.0
35	35.81685904162295	39.0	35.0	41.0	27.0	41.0
36	35.58094057044739	39.0	35.0	40.0	26.0	41.0
37	35.42272250310026	39.0	35.0	40.0	25.0	41.0
38	35.31580654392827	39.0	35.0	40.0	24.0	41.0
39	35.201373652580365	39.0	35.0	40.0	24.0	41.0
40	35.07443161944736	38.0	35.0	40.0	23.0	41.0
41	34.96111164106967	38.0	34.0	40.0	23.0	41.0
42	34.82288149066743	38.0	34.0	40.0	23.0	41.0
43	34.72065248497567	38.0	34.0	40.0	22.0	41.0
44	34.53316162676079	38.0	34.0	40.0	21.0	41.0
45	34.4456930268053	38.0	34.0	40.0	20.0	41.0
46	34.30600655028777	38.0	34.0	40.0	20.0	41.0
47	34.28018060987631	38.0	33.0	40.0	20.0	41.0
48	34.13908232376228	38.0	33.0	40.0	20.0	41.0
49	34.01167604693313	38.0	33.0	40.0	19.0	41.0
50	33.85057076536615	37.0	33.0	40.0	18.0	41.0
51	32.031008935101276	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	8.0
10	0.0
11	3.0
12	3.0
13	1.0
14	4.0
15	8.0
16	10.0
17	29.0
18	63.0
19	98.0
20	214.0
21	396.0
22	686.0
23	1124.0
24	1709.0
25	2390.0
26	2847.0
27	3020.0
28	2892.0
29	3026.0
30	3551.0
31	4295.0
32	5645.0
33	8451.0
34	11463.0
35	14526.0
36	16479.0
37	27337.0
38	28744.0
39	18213.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.28357022480842	19.800947565900344	25.16200833094852	16.753473878342714
2	35.90638812044898	24.661515469490286	24.329549429234635	15.102546980826098
3	30.136411332633788	22.485293650036567	32.016916277147125	15.361378740182518
4	27.63331107507393	24.842761296066648	30.76854589971064	16.75538172914878
5	21.130083627460333	36.3617285128303	26.29845146109574	16.20973639861363
6	23.704410315113357	34.8945912429648	28.867054596330565	12.533943845591274
7	44.88282616299406	43.619828929377725	9.078190085535311	2.419154822092912
8	78.12331075709879	5.389042576870489	13.433177525517504	3.054469140513212
9	72.34252281471589	8.241915482209292	14.747686730897644	4.6678749721771755
10	40.66393208051131	24.26150275048491	22.147604057362713	12.92696111164107
11	32.45699386307991	24.173741613405834	28.181500206683836	15.187764316830425
12	28.93573722534898	23.347006264110146	31.43120607968457	16.286050430856307
13	22.064930522433144	27.010079811758718	33.67102292600719	17.253966739800948
14	18.314731787974182	30.000317975134344	33.797577029476294	17.88737320741518
15	17.972590543419503	25.29365003656714	39.76279054977901	16.970968870234348
16	19.996184298387867	23.022035676810074	39.216509268975166	17.765270755826894
17	20.71226430093167	23.587395465674586	33.54446882253808	22.15587141085567
18	21.4900314795383	25.651690037839042	34.40045788419346	18.457820598429205
19	24.402683710133868	24.80396832967662	31.798785334986807	18.99456262520271
20	24.958504244968044	25.274571528506474	32.923781360297625	16.84314286622786
21	24.026201151069987	25.371235969347193	32.472892619797136	18.129670259785684
22	22.241724697128685	25.346433908868327	31.603548602499288	20.808292791503703
23	22.013418550669336	24.769627015167416	32.44300295716875	20.773951476994498
24	21.35012242042672	24.643708861966996	34.59315081560622	19.413017902000064
25	20.135457407230756	26.198607268911573	34.03923813157811	19.626697192279565
26	18.830487455880952	28.582784826226586	33.18579287099749	19.400934846894973
27	20.44007758593278	27.97545231962861	33.362587045693026	18.22188304874559
28	18.574835447867976	27.991987026614517	35.317498171642974	18.11567935387453
29	19.923685967757322	26.31180641673821	35.263442398804415	18.501065216700056
30	19.82320582530446	27.442525994467232	33.88088651467455	18.853381665553755
31	23.74129543069732	26.732805494610325	31.46045979204426	18.065439282648097
32	22.468758943050656	27.528379280740246	32.179719545931505	17.823142230277593
33	22.85223695507011	28.996152500874434	29.902381633756242	18.249228910299216
34	20.4159114757226	28.685172819485516	31.546313078317272	19.35260262647461
35	21.511653788673726	26.911507520111925	31.03055741041051	20.54628128080384
36	22.50246430729117	28.754491398772615	30.855035136252347	17.888009157683868
37	22.133613151451556	28.673725714649112	30.929441317688955	18.26321981621037
38	21.79210785716557	29.082005787147448	28.081020064230977	21.044866291456007
39	22.30213997265414	28.05494610321473	30.445483163216636	19.197430760914497
40	21.492575280613053	27.056504181373015	31.58764984578206	19.86327069223187
41	19.97011033737162	27.374479315717508	31.496708957359537	21.15870138955134
42	20.896689878851475	28.009157683869123	31.027377659067064	20.06677477821234
43	22.58259404114598	26.40592705650418	29.87312792139655	21.138350980953287
44	20.883970873477693	29.15514006804668	29.41079207605965	20.550096982415976
45	20.449616839963113	30.356450125600176	29.07946198607269	20.11447104836402
46	21.479220324970587	29.192025183630637	29.42605488250819	19.902699608890583
47	22.994689815256447	27.675919743076093	29.970428312505966	19.3589621291615
48	21.94346402111355	28.01996883843683	29.90174568348755	20.134821456962065
49	21.08302330757735	27.390378072434736	30.617825686031352	20.908772933956566
50	18.852109765016376	28.90457566218322	31.118318547489586	21.12499602531082
51	20.64167382110719	27.813285001112913	29.041304969951348	22.50373620782855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	346.0
2	519.0
3	2477.5
4	4436.0
5	3091.0
6	1746.0
7	1548.5
8	1351.0
9	1301.0
10	1251.0
11	1222.0
12	1193.0
13	1153.5
14	1114.0
15	1110.5
16	1107.0
17	1014.0
18	921.0
19	963.0
20	1005.0
21	1040.5
22	1076.0
23	1123.5
24	1171.0
25	1599.5
26	2243.5
27	2459.0
28	2777.5
29	3096.0
30	3524.0
31	3952.0
32	4191.5
33	4431.0
34	4881.5
35	5332.0
36	5891.5
37	6451.0
38	6621.5
39	6792.0
40	7586.0
41	8380.0
42	9415.5
43	10451.0
44	10838.5
45	11226.0
46	11401.5
47	11577.0
48	11387.0
49	11197.0
50	10390.5
51	9584.0
52	8815.0
53	8046.0
54	7407.5
55	6769.0
56	6446.0
57	6123.0
58	5731.0
59	5339.0
60	5197.0
61	5055.0
62	4398.0
63	3741.0
64	3144.0
65	2547.0
66	2187.5
67	1828.0
68	1609.5
69	1391.0
70	1147.0
71	903.0
72	758.0
73	613.0
74	503.5
75	308.5
76	223.0
77	175.0
78	127.0
79	96.0
80	65.0
81	48.5
82	32.0
83	26.0
84	20.0
85	12.0
86	4.0
87	3.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	157245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.30624185188718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84302253972163	30.088715062482112
2	7.990516073408055	6.601163788991701
3	4.021431210740238	4.983306305446914
4	2.80514841729277	4.634805558205348
5	2.189309028205444	4.521606410378708
6	1.719731494026358	4.262138700753601
7	1.4395245719916245	4.16229450856943
8	1.177792831629511	3.8920156443766096
9	0.9668678408671019	3.594390918630163
>10	4.797388840990269	29.76374447518204
>50	0.0307919694543663	0.8706159178352253
>100	0.01539598472718315	1.0480460427994531
>500	0.001539598472718315	0.5456453305351522
>1k	0.001539598472718315	1.0315113358135393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1622	1.0315113358135393	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	858	0.5456453305351522	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	319	0.20286813571178736	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	240	0.15262806448535726	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	166	0.10556774460237209	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	162	0.10302394352761614	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	159	0.10111609272154917	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027345861553626505	0.0
2	0.0	0.0	0.0	0.17234252281471588	0.0
3	0.0	0.0	0.0	0.22258259404114597	0.0
4	0.0	0.0	0.0	0.28426977010397786	0.0
5	0.0	0.0	0.0	0.5208432700562816	0.0
6	0.0	0.0	0.0	0.6709275334668829	0.0
7	0.0	0.0	0.0	0.8419981557442208	0.0
8	0.0	0.0	0.0	1.2560017806607524	0.0
9	0.0	0.0	0.0	1.4264364526694013	0.0
10	0.0	0.0	0.0	1.934560717351903	0.0
11	0.0	0.0	0.0	2.5024643072911696	0.0
12	0.0	0.0	0.0	3.043657985945499	0.0
13	0.0	0.0	0.0	3.278959585360425	0.0
14	0.0	0.0	0.0	3.3953384845305097	0.0
15	0.0	0.0	0.0	3.555597952240135	0.0
16	0.0	0.0	0.0	3.8576743298674043	0.0
17	0.0	0.0	0.0	4.239880441349486	0.0
18	0.0	0.0	0.0	4.694584883462113	0.0
19	0.0	0.0	0.0	4.945149289325575	0.0
20	0.0	0.0	0.0	5.188718242233457	0.0
21	0.0	0.0	0.0	5.489522719323349	0.0
22	0.0	0.0	0.0	5.806225953130466	0.0
23	0.0	0.0	0.0	6.134376291773983	0.0
24	0.0	0.0	0.0	6.384940697637445	0.0
25	0.0	0.0	0.0	6.603071639797768	0.0
26	0.0	0.0	0.0	6.814843079271201	0.0
27	0.0	0.0	0.0	7.022162866863812	0.0
28	0.0	0.0	0.0	7.248561162517091	0.0
29	0.0	0.0	0.0	7.469871856020859	0.0
30	0.0	0.0	0.0	7.747146173169258	0.0
31	0.0	0.0	6.359502686889885E-4	8.014881236287323	0.0
32	0.0	0.0	6.359502686889885E-4	8.28643200101752	0.0
33	0.0	0.0	6.359502686889885E-4	8.527457152850648	0.0
34	0.0	0.0	6.359502686889885E-4	8.775477757639353	0.0
35	0.0	0.0	6.359502686889885E-4	9.037489268339217	0.0
36	0.0	0.0	6.359502686889885E-4	9.349740850265508	0.0
37	0.0	0.0	6.359502686889885E-4	9.62637921714522	0.0
38	0.0	0.0	6.359502686889885E-4	9.913192788323952	0.0
39	0.0	0.0	6.359502686889885E-4	10.162485293650036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAA	20	7.014698E-4	45.000004	1
CGGGTAC	20	7.014698E-4	45.000004	6
AGGTAAT	25	3.875405E-5	45.000004	7
TTAATCG	20	7.014698E-4	45.000004	20
CGAAAAG	20	7.014698E-4	45.000004	2
TTAGCGG	25	3.875405E-5	45.000004	2
ATAATGC	20	7.014698E-4	45.000004	32
GACAAAC	20	7.014698E-4	45.000004	12
CCTAATA	20	7.014698E-4	45.000004	28
TACGGGA	20	7.014698E-4	45.000004	4
ACGGGTC	20	7.014698E-4	45.000004	5
ACGGGTA	20	7.014698E-4	45.000004	5
ACGGGCG	20	7.014698E-4	45.000004	5
TACGAAA	20	7.014698E-4	45.000004	20
GAGCGAT	20	7.014698E-4	45.000004	6
GTGTATT	20	7.014698E-4	45.000004	9
GCGATTG	25	3.875405E-5	45.000004	9
CATACGA	20	7.014698E-4	45.000004	18
CGATGCC	20	7.014698E-4	45.000004	18
GGTTAGT	20	7.014698E-4	45.000004	38
>>END_MODULE