FastQCFastQC Report
Sat 18 Jun 2016
SRR3551089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533985
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.29439029186213095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG551.8189894E-1245.0000042
GTTTCGG207.02989E-445.02
CAGTTCG207.02989E-445.040
TGTTGCG207.02989E-445.01
CGGTCTA207.02989E-445.031
AGTACGG453.8380676E-1045.02
CGGTTTT1900.040.2631571
CGTTATT1250.039.6000021
CACGTCA403.4535879E-739.37534
CTAACGG700.038.5714262
CGTTTTT6250.037.81
CACGTCG301.13910326E-437.50000441
TAATGGG3200.037.2656253
ACGGGTA850.037.0588235
CACGACG250.002106100836.026
GCACCGA751.8189894E-1236.09
CTGCGTA250.002106100836.012
CGCTAAG250.002106100836.02
TAGGGCG1000.036.05
AGTTACG250.002106100836.01
Produced by FastQC (version 0.11.5)