##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533985
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9283949923687	31.0	31.0	34.0	30.0	34.0
2	32.0656179480697	33.0	31.0	34.0	30.0	34.0
3	31.95157916420873	33.0	31.0	34.0	30.0	34.0
4	35.701836193900576	37.0	35.0	37.0	35.0	37.0
5	31.16931187205633	37.0	35.0	37.0	0.0	37.0
6	33.40536531925054	37.0	35.0	37.0	17.0	37.0
7	29.52148843132298	35.0	32.0	37.0	0.0	37.0
8	32.5859640252067	35.0	33.0	37.0	17.0	37.0
9	36.454254332986885	39.0	35.0	39.0	32.0	39.0
10	36.81529256439788	38.0	35.0	39.0	33.0	39.0
11	36.955884528591625	39.0	37.0	39.0	33.0	39.0
12	36.989741284867556	39.0	37.0	39.0	33.0	39.0
13	36.83453842336395	39.0	37.0	39.0	33.0	39.0
14	38.11017537945823	40.0	37.0	41.0	33.0	41.0
15	38.21064449375919	40.0	37.0	41.0	33.0	41.0
16	38.240920625111194	40.0	37.0	41.0	34.0	41.0
17	38.21784507055442	40.0	37.0	41.0	34.0	41.0
18	38.11525791922994	40.0	37.0	41.0	33.0	41.0
19	38.01965785555774	40.0	37.0	41.0	33.0	41.0
20	37.96343904791333	40.0	37.0	41.0	33.0	41.0
21	37.853258050319766	40.0	36.0	41.0	33.0	41.0
22	37.84318660636535	40.0	36.0	41.0	33.0	41.0
23	37.77102165791174	40.0	36.0	41.0	33.0	41.0
24	37.686388194424936	40.0	36.0	41.0	33.0	41.0
25	37.552343230615094	40.0	36.0	41.0	33.0	41.0
26	37.385804844705376	39.0	36.0	41.0	32.0	41.0
27	37.336646160472675	39.0	36.0	41.0	32.0	41.0
28	37.20850023877075	39.0	35.0	41.0	32.0	41.0
29	37.17989831175033	39.0	35.0	41.0	32.0	41.0
30	37.06782962068223	39.0	35.0	41.0	31.0	41.0
31	36.98019045478806	39.0	35.0	41.0	31.0	41.0
32	36.87317621281497	39.0	35.0	41.0	31.0	41.0
33	36.71440208994635	39.0	35.0	41.0	30.0	41.0
34	36.589705703343725	39.0	35.0	41.0	30.0	41.0
35	36.47732052398476	39.0	35.0	41.0	30.0	41.0
36	36.36056818075414	39.0	35.0	40.0	30.0	41.0
37	36.27823253462176	39.0	35.0	40.0	30.0	41.0
38	36.173237075947824	39.0	35.0	40.0	29.0	41.0
39	36.15191063419385	39.0	35.0	40.0	29.0	41.0
40	35.98898470930831	39.0	35.0	40.0	29.0	41.0
41	35.84988904182702	38.0	35.0	40.0	28.0	41.0
42	35.801951365675066	38.0	35.0	40.0	28.0	41.0
43	35.62883788870474	38.0	35.0	40.0	27.0	41.0
44	35.38734608650056	38.0	34.0	40.0	26.0	41.0
45	35.27834489732858	38.0	34.0	40.0	26.0	41.0
46	35.16972574135978	38.0	34.0	40.0	25.0	41.0
47	35.03767334288416	38.0	34.0	40.0	25.0	41.0
48	34.940032023371444	38.0	34.0	40.0	24.0	41.0
49	34.767109563002705	38.0	34.0	40.0	24.0	41.0
50	34.59956740357875	37.0	34.0	40.0	24.0	41.0
51	32.635787522121404	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	8.0
9	30.0
10	36.0
11	26.0
12	16.0
13	19.0
14	18.0
15	45.0
16	93.0
17	154.0
18	296.0
19	496.0
20	855.0
21	1316.0
22	1765.0
23	2594.0
24	3575.0
25	4671.0
26	5671.0
27	6572.0
28	7596.0
29	9274.0
30	11382.0
31	14220.0
32	18541.0
33	25913.0
34	37206.0
35	45296.0
36	53310.0
37	83003.0
38	103273.0
39	96701.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7083438673371	20.211990973529222	23.925952976207196	12.153712182926487
2	32.39341929080405	24.47109937545062	25.607460883732692	17.52802045001264
3	32.63106641572329	24.804816614698915	26.120958453889152	16.443158515688644
4	28.499302414861845	26.37583452718709	26.094553217787013	19.03030984016405
5	22.053428467091774	39.98258378044327	21.486371340018913	16.477616412446043
6	26.254670075002107	36.456080226972674	23.694860342518982	13.59438935550624
7	69.96675936589979	19.845875820481847	6.560296637545998	3.6270681760723615
8	84.54282423663587	5.191344326151484	6.832588930400667	3.433242506811989
9	77.96267685421874	7.991048437690197	9.047445152953735	4.998829555137316
10	41.005458954839554	28.730207777372023	15.977040553573602	14.28729271421482
11	31.539837261346293	28.36783804788524	23.18098823000646	16.911336460762005
12	29.815444254052082	24.35686395685272	26.944577094862215	18.883114694232983
13	25.49828178694158	25.462138449581918	28.14011629540156	20.89946346807495
14	20.95545755030572	28.68058091519425	28.022509995599126	22.341451538900905
15	21.07868198544903	27.175482457372397	31.989100817438693	19.75673473973988
16	23.51845089281534	25.635738831615118	31.215670852177496	19.63013942339204
17	23.672575072333494	25.15576280232591	28.216897478393587	22.954764646947012
18	23.737370899931644	26.36328735825913	28.209593902450443	21.689747839358784
19	26.237253855445374	27.858273172467392	25.33554313323408	20.56892983885315
20	27.916889051190573	26.781651169976683	25.560081275691264	19.741378503141473
21	26.31665683492982	27.256945419815164	26.50636253827355	19.92003520698147
22	24.676348586570786	26.38313810313024	25.228611290579323	23.711902019719655
23	24.195248930213396	27.742914126801317	26.53145687612948	21.530380066855813
24	23.868647995730218	25.812522823674822	28.81129619745873	21.50753298313623
25	23.884566045862712	27.710141670646177	26.36216373119095	22.04312855230016
26	23.062632845491915	28.64799573021714	26.214781314081858	22.074590110209087
27	23.87632611402942	28.18843225933313	26.726780714814087	21.208460911823366
28	21.869340899088925	29.055123271252935	27.95921233742521	21.116323492232926
29	23.088850810416023	27.950785134413884	26.810678202571232	22.149685852598854
30	23.477813047182973	27.770255718793603	26.687453767427925	22.064477466595502
31	23.654784310420705	27.812766276206258	25.30408157532515	23.228367838047888
32	24.682341264267723	28.252853544575224	25.50764534584305	21.557159845314004
33	24.447503207018922	28.141614464825793	25.12542487148515	22.285457456670134
34	22.628725525997922	27.845351461183366	26.621534312761597	22.90438870005712
35	23.781192355590512	26.307106004850322	26.411603322190697	23.500098317368465
36	23.057389252507093	29.36037529144077	25.652031424103672	21.930204031948463
37	22.759440808262404	28.821970654606403	26.404674288603612	22.01391424852758
38	22.665243405713646	28.300233152616645	26.198301450415272	22.836221991254433
39	22.527411818684044	27.247581860913694	26.746818730863225	23.478187589539033
40	23.603472007640665	26.049795406238	28.96429674990871	21.382435836212625
41	20.520426603743548	27.483730816408702	29.248574398157253	22.747268181690497
42	22.650448982649326	27.298145078981616	27.38934614268191	22.662059795687146
43	23.182486399430697	27.00618931243387	27.347959212337425	22.46336507579801
44	22.760002621796495	27.517065086097926	26.595690890193545	23.127241401912038
45	22.057548433008417	27.06592881822523	26.358231036452334	24.518291712314017
46	21.32793992340609	28.58432352968716	26.541382248565032	23.54635429834171
47	21.964474657527834	27.887112933883913	27.67044018090396	22.477972227684297
48	22.43508712791558	26.157850875960936	27.859771341891626	23.547290654231862
49	21.44217534200399	27.23147653960317	27.43485303894304	23.891495079449797
50	20.57810612657659	27.24720731855764	28.211653885408765	23.963032669457007
51	20.641591055928536	26.781089356442596	27.075292377126697	25.502027210502163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	356.0
1	718.0
2	1080.0
3	2328.0
4	3576.0
5	2713.0
6	1850.0
7	1574.5
8	1299.0
9	1258.0
10	1217.0
11	1230.5
12	1244.0
13	1290.0
14	1336.0
15	1296.5
16	1257.0
17	1318.0
18	1379.0
19	1479.5
20	1580.0
21	1939.0
22	2298.0
23	2459.0
24	2620.0
25	3031.5
26	4489.0
27	5535.0
28	6650.5
29	7766.0
30	9078.0
31	10390.0
32	11824.5
33	13259.0
34	14415.5
35	15572.0
36	17108.5
37	18645.0
38	20090.5
39	21536.0
40	23656.0
41	25776.0
42	28685.5
43	31595.0
44	34052.0
45	36509.0
46	38094.0
47	39679.0
48	40523.0
49	41367.0
50	40205.5
51	39044.0
52	36414.5
53	33785.0
54	31029.5
55	28274.0
56	27675.5
57	27077.0
58	26612.0
59	26147.0
60	25481.5
61	24816.0
62	22489.5
63	20163.0
64	16932.5
65	13702.0
66	11435.0
67	9168.0
68	7757.0
69	6346.0
70	5318.0
71	4290.0
72	3791.0
73	3292.0
74	2828.5
75	1941.5
76	1518.0
77	1153.5
78	789.0
79	620.5
80	452.0
81	348.5
82	245.0
83	188.0
84	131.0
85	108.5
86	86.0
87	70.5
88	55.0
89	49.5
90	44.0
91	32.5
92	21.0
93	14.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.46480042633069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.49355760692777	26.784918675119258
2	9.034291386678321	6.7693584759044345
3	4.497066389691662	5.054450843812728
4	2.918537499758255	4.3736969986082075
5	2.0859805194918426	3.907542192798775
6	1.5051057804921588	3.383309261199325
7	1.0674475202256084	2.799419581758381
8	0.8796451086008905	2.636458275178428
9	0.6885664940905951	2.3217305653226123
>10	5.775368792965646	39.76720811035765
>50	0.039092719864686654	0.9386423542993082
>100	0.01484533665731712	0.9718276162737934
>500	0.0	0.0
>1k	4.94844555243904E-4	0.2914370493671124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.29439029186213095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.872711780293454E-4	0.0	0.0	0.04119965916645599	0.0
2	1.872711780293454E-4	0.0	0.0	0.18989297452175624	0.0
3	1.872711780293454E-4	0.0	0.0	0.2702323098963454	0.0
4	1.872711780293454E-4	0.0	0.0	0.3840931861381874	0.0
5	1.872711780293454E-4	0.0	0.0	0.7771753888217834	0.0
6	1.872711780293454E-4	0.0	0.0	1.09853273032014	0.0
7	1.872711780293454E-4	0.0	0.0	1.3899266833338015	0.0
8	1.872711780293454E-4	0.0	0.0	2.0161615026639326	0.0
9	1.872711780293454E-4	0.0	0.0	2.3240353193441763	0.0
10	1.872711780293454E-4	0.0	0.0	2.968997256477242	0.0
11	1.872711780293454E-4	0.0	0.0	3.565455958500707	0.0
12	1.872711780293454E-4	0.0	0.0	4.0995533582404	0.0
13	1.872711780293454E-4	0.0	0.0	4.320346077136998	0.0
14	1.872711780293454E-4	0.0	0.0	4.427090648613725	0.0
15	1.872711780293454E-4	0.0	0.0	4.586458421116698	0.0
16	1.872711780293454E-4	0.0	0.0	4.890961356592413	0.0
17	1.872711780293454E-4	0.0	0.0	5.245278425423935	0.0
18	1.872711780293454E-4	0.0	0.0	5.678062117849752	0.0
19	1.872711780293454E-4	0.0	0.0	5.917581954549285	0.0
20	1.872711780293454E-4	0.0	0.0	6.15373091004429	0.0
21	1.872711780293454E-4	0.0	0.0	6.471717370338118	0.0
22	1.872711780293454E-4	0.0	0.0	6.811427287283351	0.0
23	1.872711780293454E-4	0.0	0.0	7.153946271899024	0.0
24	1.872711780293454E-4	0.0	0.0	7.431107615382454	0.0
25	1.872711780293454E-4	0.0	0.0	7.686170959858423	0.0
26	1.872711780293454E-4	0.0	0.0	7.917450864724665	0.0
27	1.872711780293454E-4	0.0	0.0	8.154910718465874	0.0
28	1.872711780293454E-4	0.0	0.0	8.403981385244904	0.0
29	1.872711780293454E-4	0.0	0.0	8.665224678595841	0.0
30	1.872711780293454E-4	0.0	0.0	8.972911224098056	0.0
31	1.872711780293454E-4	0.0	0.0	9.262807007687481	0.0
32	1.872711780293454E-4	0.0	0.0	9.545211944155735	0.0
33	1.872711780293454E-4	0.0	0.0	9.82106238939296	0.0
34	1.872711780293454E-4	0.0	0.0	10.097849190520334	0.0
35	1.872711780293454E-4	0.0	0.0	10.395797634765021	0.0
36	1.872711780293454E-4	0.0	0.0	10.693371536653652	0.0
37	1.872711780293454E-4	0.0	0.0	10.977461913724168	0.0
38	3.745423560586908E-4	0.0	0.0	11.266983154957536	0.0
39	3.745423560586908E-4	0.0	0.0	11.581973276402895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	55	1.8189894E-12	45.000004	2
GTTTCGG	20	7.02989E-4	45.0	2
CAGTTCG	20	7.02989E-4	45.0	40
TGTTGCG	20	7.02989E-4	45.0	1
CGGTCTA	20	7.02989E-4	45.0	31
AGTACGG	45	3.8380676E-10	45.0	2
CGGTTTT	190	0.0	40.263157	1
CGTTATT	125	0.0	39.600002	1
CACGTCA	40	3.4535879E-7	39.375	34
CTAACGG	70	0.0	38.571426	2
CGTTTTT	625	0.0	37.8	1
CACGTCG	30	1.13910326E-4	37.500004	41
TAATGGG	320	0.0	37.265625	3
ACGGGTA	85	0.0	37.058823	5
CACGACG	25	0.0021061008	36.0	26
GCACCGA	75	1.8189894E-12	36.0	9
CTGCGTA	25	0.0021061008	36.0	12
CGCTAAG	25	0.0021061008	36.0	2
TAGGGCG	100	0.0	36.0	5
AGTTACG	25	0.0021061008	36.0	1
>>END_MODULE