FastQCFastQC Report
Sat 18 Jun 2016
SRR3551088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367154
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC21520.5861300707604983No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC20670.5629790224265567No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG19570.5330188422296911No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTT7100.19337934490704173No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC5870.15987841614145562No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1470772482391585No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT4180.11384868474808936No Hit
GAATATGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC3980.10840137925775015No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC3900.10622245706161447No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC3830.10431590013999575No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG3780.10295407376741095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT700.045.0000041
CGAGCAC351.2089004E-745.00000425
TCGCCAC253.8856022E-545.043
CGTATCG207.0270087E-445.028
CCGATTC207.0270087E-445.010
ACCGAAG253.8856022E-545.032
TGACCGA253.8856022E-545.030
TCTCGAA207.0270087E-445.032
TCGTAAG207.0270087E-445.01
CTCGAAA207.0270087E-445.033
TTACGAG207.0270087E-445.025
AACTCGT207.0270087E-445.07
CGTCATA253.8856022E-545.038
TCGCGTT253.8856022E-545.036
TACACGA207.0270087E-445.026
TACGTGG207.0270087E-445.02
CGCTTAG207.0270087E-445.02
TGCGTAG207.0270087E-445.01
TGGTACG207.0270087E-445.01
TATGCGG302.1614524E-644.9999962