FastQCFastQC Report
Sat 18 Jun 2016
SRR3551085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269435
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG31911.1843301723977955No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC28171.0455211832167313No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC23920.8877836955109767No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC8040.2984022120362982TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT6680.24792621597045672No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG6640.24644162785087315No Hit
GAATATGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC5260.19522333772523986No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC5060.187800397127322No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC4930.18297548573867536No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTT4770.1770371332603411No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG4590.170356486722215No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC4190.15551060552637927No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTT3530.13101490155325032No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT3440.1276745782841873TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.12581884313470781No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2970.1102306678790803No Hit
GAATCTATCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC2880.10689034461001726No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA2750.10206543322137064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG502.1827873E-1145.0000042
GCGTTGG253.8828293E-545.0000042
CGGGAGC253.8828293E-545.0000046
CGATACA253.8828293E-545.00000413
TCTATCT950.045.0000044
TAATACG800.045.04
CGAGTGA207.023662E-445.014
CGTGCGG207.023662E-445.02
CGGCATC207.023662E-445.041
ACCGACC207.023662E-445.030
ACGGGTG406.7811925E-945.05
ACGGGAC351.2072996E-745.05
CATAGCG207.023662E-445.01
GTGTACG207.023662E-445.01
AGCGAGT207.023662E-445.012
GCGATAT351.2072996E-745.09
GGTTGAC207.023662E-445.08
GTTGGCA207.023662E-445.029
AGAGCGA207.023662E-445.010
ACGACTC207.023662E-445.020
Produced by FastQC (version 0.11.5)