FastQCFastQC Report
Sat 18 Jun 2016
SRR3551082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104744
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55420.5016546819896737No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC35660.32278971417812635No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG31780.2876684553163448No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC26420.23915042761037852No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18820.17035620922132186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC207.0331676E-445.044
TCGCAAT207.0331676E-445.016
GATCCGC207.0331676E-445.09
CGTCTCA207.0331676E-445.033
GCCGATT207.0331676E-445.09
AGCGATA253.8907063E-545.01
TTACGCG207.0331676E-445.01
GCCTACG302.165425E-644.9999961
CGTTATT5050.044.1089131
TACGGGA5100.043.2352944
CGTTTTA2600.041.5384641
CGTTTTT24850.041.2877271
GCGCTAG603.6379788E-1241.2499961
TAGTACG603.6379788E-1241.2499961
CTACGGG2550.040.5882343
CGGTTTT6150.040.24391
TACGGCT4600.040.10877
TCTACGG451.9281288E-840.02
CGCACGG1750.039.8571432
GCGAACG800.039.3751