##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551082_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1104744
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.858207874403483	31.0	31.0	34.0	30.0	34.0
2	31.967799779858503	33.0	31.0	34.0	30.0	34.0
3	31.743536964219764	33.0	31.0	34.0	30.0	34.0
4	35.55199123054753	37.0	35.0	37.0	33.0	37.0
5	31.042476809106905	37.0	35.0	37.0	0.0	37.0
6	33.31800308487758	37.0	35.0	37.0	17.0	37.0
7	29.704937976581	35.0	32.0	37.0	0.0	37.0
8	32.69423866524733	35.0	35.0	37.0	17.0	37.0
9	36.55457101373712	39.0	35.0	39.0	32.0	39.0
10	36.88546667825306	38.0	37.0	39.0	33.0	39.0
11	36.94086774854627	39.0	37.0	39.0	33.0	39.0
12	36.87174223168444	39.0	35.0	39.0	33.0	39.0
13	36.68623771661127	39.0	35.0	39.0	33.0	39.0
14	37.92295500133968	40.0	36.0	41.0	33.0	41.0
15	37.93681975190633	40.0	36.0	41.0	33.0	41.0
16	37.95799298298972	40.0	36.0	41.0	33.0	41.0
17	37.84386246949519	40.0	36.0	41.0	33.0	41.0
18	37.68414945000833	40.0	36.0	41.0	33.0	41.0
19	37.60976117543974	39.0	36.0	41.0	32.0	41.0
20	37.58131567132295	39.0	35.0	41.0	33.0	41.0
21	37.50473955957217	39.0	35.0	41.0	33.0	41.0
22	37.51659479481219	39.0	35.0	41.0	33.0	41.0
23	37.49047019037895	39.0	35.0	41.0	33.0	41.0
24	37.48095938968666	39.0	35.0	41.0	33.0	41.0
25	37.400906454345986	39.0	35.0	41.0	33.0	41.0
26	37.256142599552476	39.0	35.0	41.0	32.0	41.0
27	37.25909350944653	39.0	35.0	41.0	33.0	41.0
28	37.15187047859052	39.0	35.0	41.0	32.0	41.0
29	37.13415144141991	39.0	35.0	41.0	32.0	41.0
30	36.97582516854583	39.0	35.0	41.0	32.0	41.0
31	36.84475498396009	39.0	35.0	41.0	31.0	41.0
32	36.71787038445106	39.0	35.0	41.0	31.0	41.0
33	36.51140988319466	39.0	35.0	40.0	30.0	41.0
34	36.215360300666944	39.0	35.0	40.0	30.0	41.0
35	36.04134894600016	39.0	35.0	40.0	29.0	41.0
36	36.006188764093764	39.0	35.0	40.0	29.0	41.0
37	35.98992074181892	39.0	35.0	40.0	29.0	41.0
38	35.87313169385849	38.0	35.0	40.0	29.0	41.0
39	35.81845567841962	39.0	35.0	40.0	29.0	41.0
40	35.67401135466678	38.0	35.0	40.0	27.0	41.0
41	35.587848406508655	38.0	35.0	40.0	27.0	41.0
42	35.5448348214609	38.0	35.0	40.0	27.0	41.0
43	35.393818839477746	38.0	34.0	40.0	26.0	41.0
44	35.22806098064348	38.0	34.0	40.0	25.0	41.0
45	35.072598719703386	38.0	34.0	40.0	24.0	41.0
46	34.852392047388356	38.0	34.0	40.0	24.0	41.0
47	34.69718233364472	38.0	34.0	40.0	23.0	41.0
48	34.63674570760285	38.0	34.0	40.0	23.0	41.0
49	34.48134137863614	38.0	34.0	40.0	23.0	41.0
50	34.3605586452608	37.0	33.0	40.0	23.0	41.0
51	32.4144607257428	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	45.0
10	52.0
11	39.0
12	25.0
13	28.0
14	49.0
15	78.0
16	144.0
17	280.0
18	476.0
19	863.0
20	1471.0
21	2368.0
22	3556.0
23	5415.0
24	8255.0
25	11854.0
26	14855.0
27	16838.0
28	18927.0
29	21395.0
30	25844.0
31	32194.0
32	40861.0
33	60168.0
34	84773.0
35	94368.0
36	98404.0
37	157713.0
38	202422.0
39	200937.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06490915542424	19.573403431021124	24.151387108687626	13.21030030486701
2	32.60357150615889	25.082372024650056	24.846299233125503	17.46775723606555
3	31.622620263155987	25.57035838167032	27.069891305134945	15.737130050038742
4	26.26318857581485	25.224576915556906	29.322268326417706	19.18996618221054
5	22.16232901015982	38.90077701259296	22.73277791053855	16.204116066708668
6	24.670964494941813	34.48889516485267	26.77009334289211	14.070046997313405
7	69.3540766005518	18.276089302136967	8.912381511010697	3.4574525863005365
8	83.47164591977871	4.64858827022369	8.778775897402475	3.1009899125951352
9	78.07283859428067	6.257739349568769	10.057262134938048	5.612159921212516
10	39.77373943646673	32.78976848935138	16.383795702895874	11.052696371286018
11	31.325537862165355	23.764600667665995	28.79354855061444	16.11631291955421
12	28.646817724287253	23.19170776216028	30.123087339691367	18.038387173861093
13	25.090790264531876	23.711556704539692	32.07349394972953	19.1241590811989
14	20.72634021999667	26.914923276342755	31.606688970476416	20.75204753318416
15	21.22374052269123	25.52600421455106	35.64644840795695	17.603806854800748
16	27.044002954530644	27.03965805652712	29.837319777251565	16.079019211690674
17	25.637975856850094	25.648657064442077	30.2690940163513	18.44427306235653
18	26.51908496448046	25.60231148573787	30.881091003888685	16.997512545892985
19	24.6258861781553	28.8582694271252	28.454827543756743	18.06101685096276
20	27.136965668064278	29.402829976899625	28.306648418095055	15.153555936941046
21	25.36759647483942	30.066784703062428	28.870217896634877	15.695400925463273
22	24.002302795941866	25.30468597249679	31.429634376833004	19.263376854728335
23	22.950656441673363	27.707233531026194	31.724453810113474	17.61765621718697
24	24.54116066708667	24.243987747387628	31.27367064224834	19.941180943277356
25	22.933548405784506	27.214992794710813	28.89601572853077	20.95544307097391
26	21.32593614448234	25.70459762623739	30.70829078954038	22.261175439739887
27	20.44690896714533	26.856176634586838	30.60283649424663	22.094077904021205
28	17.816073226014353	29.123670280173506	30.93694104697559	22.123315446836553
29	22.407996784775477	28.621563004641796	28.549238556624886	20.42120165395784
30	22.717570767526233	25.198235971410572	32.1710731173919	19.913120143671296
31	24.48829774137719	26.343931263713582	29.428718327503926	19.7390526674053
32	25.192352255364135	27.010511032420183	29.635734613629943	18.161402098585736
33	24.02764803429573	25.978869312709552	30.180928794363222	19.812553858631503
34	21.445330320870717	27.201233951033	33.07399723374827	18.279438494348014
35	18.55778352269847	30.416367954928923	32.19705198670461	18.82879653566799
36	18.6343623500105	34.092151665906314	28.280397992657125	18.99308799142607
37	21.329918967652233	32.09947281904224	27.675551983083864	18.895056230221662
38	21.14372198445975	32.45530186178879	27.129090540432898	19.27188561331856
39	23.169168603767027	28.686012325027338	27.108542793624586	21.036276277581052
40	23.23017821323311	26.20996357527174	28.93376202993635	21.626096181558804
41	19.47048365956276	28.549510112750102	27.365344369374263	24.614661858312875
42	20.643696639221396	28.038984597336576	27.892887401968242	23.42443136147379
43	20.81450544198475	25.766240866662322	28.06342464860637	25.355829042746553
44	20.917334694734706	26.039697884758823	28.67107673814024	24.37189068236623
45	22.103672887112307	25.94374805384777	28.390468742079612	23.562110316960307
46	21.288008805659953	28.410473376637484	27.305420984409057	22.996096833293507
47	19.31605874302101	27.565662271078185	30.29480132953879	22.82347765636202
48	20.400201313607496	26.378509410324924	30.580297335853373	22.640991940214203
49	22.13037590609227	24.781306800489524	31.24778229164404	21.840535001774168
50	19.67433179089454	26.416255711730503	30.54544763311681	23.363964864258143
51	19.299041225840558	24.804117515008002	30.207088701092744	25.6897525580587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	525.0
1	1016.5
2	1508.0
3	7305.5
4	13103.0
5	10028.5
6	6954.0
7	5423.5
8	3893.0
9	3734.5
10	3576.0
11	3538.5
12	3501.0
13	3525.0
14	3549.0
15	3460.5
16	3372.0
17	3360.0
18	3348.0
19	3605.0
20	3862.0
21	4435.5
22	5009.0
23	5604.5
24	6200.0
25	7978.0
26	11941.5
27	14127.0
28	16625.5
29	19124.0
30	21191.0
31	23258.0
32	27012.0
33	30766.0
34	35002.0
35	39238.0
36	41790.5
37	44343.0
38	47644.0
39	50945.0
40	55268.5
41	59592.0
42	66198.0
43	72804.0
44	84036.5
45	95269.0
46	90651.5
47	86034.0
48	84348.5
49	82663.0
50	78528.0
51	74393.0
52	70873.0
53	67353.0
54	63255.5
55	59158.0
56	55264.5
57	51371.0
58	50328.5
59	49286.0
60	46400.5
61	43515.0
62	38212.0
63	32909.0
64	26710.5
65	20512.0
66	15167.0
67	9822.0
68	7159.5
69	4497.0
70	3387.5
71	2278.0
72	1754.5
73	1231.0
74	1028.5
75	682.5
76	539.0
77	388.5
78	238.0
79	213.5
80	189.0
81	150.5
82	112.0
83	92.0
84	72.0
85	57.0
86	42.0
87	35.5
88	29.0
89	21.5
90	14.0
91	12.5
92	11.0
93	13.0
94	15.0
95	13.5
96	12.0
97	6.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1104744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.98744754815761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59781487999867	25.76622607556651
2	9.032236470483184	6.500942724481397
3	4.424429701915581	4.776717954845927
4	2.796917546458828	4.026156939988351
5	2.0077304629843606	3.612654736374394
6	1.5245280927333107	3.2918324863759674
7	1.1545813151315774	2.908530416286633
8	0.8963771417110659	2.5806660296555544
9	0.700360752750669	2.26837762689626
>10	5.78161035010162	39.25324208458327
>50	0.05961920735506451	1.3370334595349271
>100	0.01918877291255734	1.2624265344168188
>500	0.00281435333886319	0.6733371165551653
>1k	0.001535101821198104	1.231581740878147
>5k	2.558503035330173E-4	0.5102740735606079
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5542	0.5016546819896737	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	3566	0.32278971417812635	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	3178	0.2876684553163448	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	2642	0.23915042761037852	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1882	0.17035620922132186	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8103741681330698E-4	0.0	0.0	0.05530693083646528	0.0
2	1.8103741681330698E-4	0.0	0.0	0.2729139058460603	0.0
3	1.8103741681330698E-4	0.0	0.0	0.38035961272475793	0.0
4	1.8103741681330698E-4	0.0	0.0	0.6285619111758018	0.0
5	1.8103741681330698E-4	0.0	0.0	1.5324817333246434	0.0
6	1.8103741681330698E-4	0.0	0.0	2.0108731072538073	0.0
7	1.8103741681330698E-4	0.0	0.0	2.5032043622775957	0.0
8	1.8103741681330698E-4	0.0	0.0	3.2527897865930933	0.0
9	1.8103741681330698E-4	0.0	0.0	3.678318234812771	0.0
10	1.8103741681330698E-4	0.0	0.0	4.814056469191052	0.0
11	1.8103741681330698E-4	0.0	0.0	5.460360047214558	0.0
12	1.8103741681330698E-4	0.0	0.0	6.323636969288812	0.0
13	1.8103741681330698E-4	0.0	0.0	6.569123706487657	0.0
14	1.8103741681330698E-4	0.0	0.0	6.698203384675545	0.0
15	1.8103741681330698E-4	0.0	0.0	6.903409296633428	0.0
16	1.8103741681330698E-4	0.0	0.0	7.200763253749285	0.0
17	1.8103741681330698E-4	0.0	0.0	7.568450247297111	0.0
18	1.8103741681330698E-4	0.0	0.0	7.996694256768989	0.0
19	1.8103741681330698E-4	0.0	0.0	8.282642856625607	0.0
20	1.8103741681330698E-4	0.0	0.0	8.587057272997184	0.0
21	1.8103741681330698E-4	0.0	0.0	8.930485252692026	0.0
22	1.8103741681330698E-4	0.0	0.0	9.319534661423823	0.0
23	1.8103741681330698E-4	0.0	0.0	9.708674588864026	0.0
24	1.8103741681330698E-4	0.0	0.0	10.021054651575387	0.0
25	1.8103741681330698E-4	0.0	0.0	10.293425445171009	0.0
26	1.8103741681330698E-4	0.0	0.0	10.565343645224596	0.0
27	1.8103741681330698E-4	0.0	0.0	10.829024642813177	0.0
28	1.8103741681330698E-4	0.0	0.0	11.117960360047215	0.0
29	1.8103741681330698E-4	0.0	0.0	11.415223798454665	0.0
30	1.8103741681330698E-4	0.0	0.0	11.78861347063211	0.0
31	1.8103741681330698E-4	0.0	0.0	12.101174570760286	0.0
32	1.8103741681330698E-4	0.0	0.0	12.433378230612703	0.0
33	2.715561252199605E-4	0.0	0.0	12.773638055513313	0.0
34	2.715561252199605E-4	0.0	0.0	13.09887177481842	0.0
35	2.715561252199605E-4	0.0	0.0	13.447187764767222	0.0
36	2.715561252199605E-4	0.0	0.0	13.806275481016417	0.0
37	2.715561252199605E-4	0.0	0.0	14.169074464310283	0.0
38	2.715561252199605E-4	0.0	0.0	14.60573671366398	0.0
39	3.6207483362661396E-4	0.0	0.0	15.084128087593143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGC	20	7.0331676E-4	45.0	44
TCGCAAT	20	7.0331676E-4	45.0	16
GATCCGC	20	7.0331676E-4	45.0	9
CGTCTCA	20	7.0331676E-4	45.0	33
GCCGATT	20	7.0331676E-4	45.0	9
AGCGATA	25	3.8907063E-5	45.0	1
TTACGCG	20	7.0331676E-4	45.0	1
GCCTACG	30	2.165425E-6	44.999996	1
CGTTATT	505	0.0	44.108913	1
TACGGGA	510	0.0	43.235294	4
CGTTTTA	260	0.0	41.538464	1
CGTTTTT	2485	0.0	41.287727	1
GCGCTAG	60	3.6379788E-12	41.249996	1
TAGTACG	60	3.6379788E-12	41.249996	1
CTACGGG	255	0.0	40.588234	3
CGGTTTT	615	0.0	40.2439	1
TACGGCT	460	0.0	40.1087	7
TCTACGG	45	1.9281288E-8	40.0	2
CGCACGG	175	0.0	39.857143	2
GCGAACG	80	0.0	39.375	1
>>END_MODULE