##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299187
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.891693155117032	31.0	31.0	34.0	30.0	34.0
2	32.0315655426205	33.0	31.0	34.0	30.0	34.0
3	31.989464782894977	33.0	31.0	34.0	30.0	34.0
4	35.71351362191539	37.0	35.0	37.0	35.0	37.0
5	31.127829751961148	37.0	35.0	37.0	0.0	37.0
6	33.374267598525336	37.0	35.0	37.0	17.0	37.0
7	29.56445968574838	35.0	32.0	37.0	0.0	37.0
8	32.616022754999385	35.0	33.0	37.0	17.0	37.0
9	36.421532352675754	39.0	35.0	39.0	32.0	39.0
10	36.81772603756179	38.0	35.0	39.0	33.0	39.0
11	36.95336361539773	39.0	37.0	39.0	33.0	39.0
12	36.87875475872949	39.0	35.0	39.0	33.0	39.0
13	36.63589995554619	39.0	35.0	39.0	33.0	39.0
14	37.85919508534796	40.0	37.0	41.0	33.0	41.0
15	38.01592315174122	40.0	37.0	41.0	33.0	41.0
16	38.07423116646111	40.0	37.0	41.0	33.0	41.0
17	38.063635786314244	40.0	37.0	41.0	33.0	41.0
18	37.99944516305855	40.0	36.0	41.0	33.0	41.0
19	37.920106822823186	40.0	36.0	41.0	33.0	41.0
20	37.85786815603619	40.0	36.0	41.0	33.0	41.0
21	37.76298101187552	40.0	36.0	41.0	33.0	41.0
22	37.751770631745366	40.0	36.0	41.0	33.0	41.0
23	37.69698215497331	39.0	36.0	41.0	33.0	41.0
24	37.63422875993943	39.0	36.0	41.0	33.0	41.0
25	37.5309722681801	39.0	35.0	41.0	33.0	41.0
26	37.35437034363124	39.0	35.0	41.0	32.0	41.0
27	37.270195563309905	39.0	35.0	41.0	32.0	41.0
28	37.161250321705154	39.0	35.0	41.0	32.0	41.0
29	37.096959426713056	39.0	35.0	41.0	32.0	41.0
30	36.912773616500715	39.0	35.0	41.0	31.0	41.0
31	36.78688245144341	39.0	35.0	41.0	31.0	41.0
32	36.64753816175168	39.0	35.0	41.0	30.0	41.0
33	36.47698930769051	39.0	35.0	40.0	30.0	41.0
34	36.35135216436543	39.0	35.0	40.0	30.0	41.0
35	36.20332434230097	39.0	35.0	40.0	30.0	41.0
36	36.029276004639236	39.0	35.0	40.0	29.0	41.0
37	35.85728657996504	38.0	35.0	40.0	28.0	41.0
38	35.78420519608138	38.0	35.0	40.0	28.0	41.0
39	35.76483938139023	38.0	35.0	40.0	27.0	41.0
40	35.60390992924158	38.0	35.0	40.0	27.0	41.0
41	35.507224578608025	38.0	35.0	40.0	26.0	41.0
42	35.44958838452205	38.0	34.0	40.0	26.0	41.0
43	35.194817956662554	38.0	34.0	40.0	25.0	41.0
44	34.98690451122542	38.0	34.0	40.0	24.0	41.0
45	34.80383840206961	38.0	34.0	40.0	23.0	41.0
46	34.63452289036623	38.0	34.0	40.0	23.0	41.0
47	34.49226737792752	38.0	33.0	40.0	23.0	41.0
48	34.40115379344691	38.0	33.0	40.0	23.0	41.0
49	34.23281425997787	37.0	33.0	40.0	22.0	41.0
50	34.077804182668366	37.0	33.0	40.0	22.0	41.0
51	31.991938152392986	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	20.0
10	19.0
11	6.0
12	8.0
13	9.0
14	12.0
15	18.0
16	35.0
17	70.0
18	146.0
19	220.0
20	451.0
21	689.0
22	1113.0
23	1552.0
24	2316.0
25	2926.0
26	3542.0
27	4238.0
28	4934.0
29	5823.0
30	7032.0
31	8699.0
32	11139.0
33	15727.0
34	22574.0
35	27235.0
36	28522.0
37	44068.0
38	54412.0
39	51624.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.582775989598474	21.72052930107257	23.597616206586515	11.099078502742433
2	31.976990978886118	25.026822689488515	24.66851835139896	18.327667980226416
3	33.49176267685428	23.89007543776969	25.9379585342946	16.68020335108143
4	29.380287245100888	26.61679818976092	25.47470311210046	18.52821145303773
5	22.181445049417253	40.79355052191439	20.400953249974098	16.62405117869426
6	25.097681383215182	37.99128972849756	22.672108079562282	14.238920808724979
7	68.54107965920979	20.342795642858814	6.835858509895148	4.2802661880362445
8	82.15163091979264	6.08315200861	7.657418270178851	4.107798801418511
9	75.37727240822629	9.0030649727428	9.715328540344332	5.904334078686574
10	41.3129581164957	28.80071660867618	16.925534866153942	12.960790408674175
11	32.520463790204786	25.593358000180487	23.899768372288904	17.98640983732582
12	30.14368939826931	23.980654239656136	28.026953042745838	17.848703319328717
13	23.704572725419222	27.730817181227795	28.750580740473346	19.814029352879636
14	18.83404024907499	31.55651816422505	28.280306296730807	21.32913528996915
15	19.306320127545646	27.556010120760593	34.44802080304291	18.689648948650845
16	21.531684197508582	25.610738434490806	33.69030071493748	19.167276653063134
17	20.955790191418743	25.283184095565648	28.855197585456587	24.90582812755902
18	21.6483336508605	26.93733350713768	29.91841223047793	21.495920611523896
19	24.268434123140377	27.53395033875135	27.043621547727675	21.1539939903806
20	25.853061797471145	27.633553596914307	27.29229545401371	19.221089151600836
21	23.597281967465165	27.891920437719552	27.789309027464427	20.721488567350853
22	22.0063037498287	26.628496559008248	26.832048183911734	24.53315150725132
23	21.444113547714306	28.67604541641181	27.344770996066003	22.53507003980788
24	21.885977666141912	26.549281887247773	29.79842038591249	21.766320060697826
25	20.67369237299749	27.867855220982197	28.258914992964268	23.19953741305605
26	20.518939659811423	29.400341592382023	28.262257384177786	21.818461363628767
27	21.00057823367994	28.74456443628901	29.320458442378843	20.934398887652204
28	19.552320120860866	28.028624238352602	30.494974714810468	21.92408092597606
29	20.797695087019154	27.656616096287607	29.554425827325385	21.991262989367854
30	21.344844528672702	28.21914053752336	29.318453007650735	21.117561926153208
31	23.685186856380792	27.635559031642416	26.684648731395416	21.994605380581376
32	22.477915150056653	29.30775735576746	26.865472096046954	21.34885539812893
33	22.91409720342127	28.713480198003253	26.093045486602023	22.279377111973446
34	20.22079836356526	27.79733076637688	28.76729269654096	23.214578173516898
35	21.710502127432008	26.676961231604313	27.54063512117839	24.071901519785285
36	22.105238529748952	28.16031445216537	27.904621524330935	21.829825493754743
37	20.81106465187324	28.974855190900673	28.923048127091082	21.291032030135
38	20.54601302864095	29.330819855140767	26.112765594761804	24.01040152145648
39	21.219170619044277	27.65995848750113	27.826409569934523	23.29446132352007
40	21.962184185810212	26.848760139979344	28.79369758712778	22.395358087082663
41	19.284260345536403	27.080053611955062	28.48653183460511	25.14915420790342
42	20.485849986797554	26.811993836630606	26.872156878473994	25.829999298097846
43	22.62431188520892	26.249803634516205	27.32104001845	23.804844461824878
44	20.448080966084756	28.41433618439304	26.876167747930225	24.261415101591982
45	19.880877177150076	28.685069872688317	26.775896011524562	24.658156938637042
46	21.01294508116997	29.148325294882465	26.98947481006862	22.849254813878943
47	20.682716829274	26.839067205460132	29.15868670764438	23.31952925762149
48	20.524621724874407	25.465678655823947	29.178741054925517	24.830958564376125
49	19.985159783011962	26.050931357311647	29.28469485639416	24.67921400328223
50	18.49579025826657	26.684314492274062	30.232597004548996	24.587298244910375
51	19.05864893862367	26.0051405976864	27.61316501051182	27.323045453178114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	360.0
1	572.5
2	785.0
3	925.5
4	1066.0
5	870.0
6	674.0
7	618.5
8	563.0
9	630.5
10	698.0
11	702.0
12	706.0
13	691.5
14	677.0
15	705.5
16	734.0
17	697.0
18	660.0
19	698.0
20	736.0
21	939.0
22	1142.0
23	1338.5
24	1535.0
25	2119.0
26	3037.5
27	3372.0
28	4161.0
29	4950.0
30	5673.5
31	6397.0
32	7400.0
33	8403.0
34	8664.5
35	8926.0
36	10052.5
37	11179.0
38	11973.0
39	12767.0
40	14388.5
41	16010.0
42	18241.0
43	20472.0
44	22209.5
45	23947.0
46	25114.5
47	26282.0
48	26286.0
49	26290.0
50	25274.5
51	24259.0
52	21796.0
53	19333.0
54	17362.0
55	15391.0
56	13888.5
57	12386.0
58	11803.5
59	11221.0
60	11180.5
61	11140.0
62	9839.5
63	8539.0
64	6910.0
65	5281.0
66	4260.0
67	3239.0
68	2774.5
69	2310.0
70	1985.0
71	1660.0
72	1269.0
73	878.0
74	746.5
75	499.5
76	384.0
77	288.5
78	193.0
79	153.0
80	113.0
81	95.0
82	77.0
83	75.5
84	74.0
85	49.5
86	25.0
87	18.0
88	11.0
89	11.0
90	11.0
91	9.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	299187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.801472209043894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10196605725538	25.09753589598167
2	10.224449117907762	7.321006618951162
3	4.597181682154256	4.937576167007137
4	2.8813142880335527	4.1262117363421735
5	2.0648120142036777	3.6961654971706457
6	1.3714917570159757	2.9460854414244135
7	1.0718484326338467	2.6861626311273543
8	0.8538853133028509	2.44562810511382
9	0.65185716011212	2.100370140181831
>10	6.096602083188809	41.544902114350904
>50	0.05772166427201608	1.374948765227048
>100	0.026870429919731622	1.7234068871218533
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	391	0.13068749644870933	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	322	0.10762499707540768	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	304	0.10160869289106812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15441847406471537	0.0
2	0.0	0.0	0.0	0.6711521556752131	0.0
3	0.0	0.0	0.0	0.9101331274420346	0.0
4	0.0	0.0	0.0	1.1487798600875039	0.0
5	0.0	0.0	0.0	1.958641251123879	0.0
6	0.0	0.0	0.0	2.435600477293465	0.0
7	0.0	0.0	0.0	2.931611333380127	0.0
8	0.0	0.0	0.0	3.7829183754641744	0.0
9	0.0	0.0	0.0	4.141891191796435	0.0
10	0.0	0.0	0.0	4.976486277812873	0.0
11	0.0	0.0	0.0	6.132285159448773	0.0
12	0.0	0.0	0.0	7.0043150270566565	0.0
13	0.0	0.0	0.0	7.40172534234442	0.0
14	0.0	0.0	0.0	7.577200881054324	0.0
15	0.0	0.0	0.0	7.866986199266679	0.0
16	0.0	0.0	0.0	8.567885636742238	0.0
17	0.0	0.0	0.0	9.331956268153363	0.0
18	0.0	0.0	0.0	10.181592114630648	0.0
19	0.0	0.0	0.0	10.693312209420863	0.0
20	0.0	0.0	0.0	11.150217088309319	0.0
21	0.0	0.0	0.0	11.733464355068904	0.0
22	0.0	0.0	0.0	12.317714339192545	0.0
23	0.0	0.0	0.0	12.917005083777036	0.0
24	0.0	0.0	0.0	13.429393656809955	0.0
25	0.0	0.0	0.0	13.846189841136146	0.0
26	0.0	0.0	0.0	14.24827950412284	0.0
27	0.0	0.0	0.0	14.65438003656576	0.0
28	0.0	0.0	0.0	15.051790351853523	0.0
29	0.0	0.0	0.0	15.483627296640563	0.0
30	0.0	0.0	0.0	15.949556631805526	0.0
31	0.0	0.0	0.0	16.41849411906266	0.0
32	0.0	0.0	0.0	16.86436910694649	0.0
33	0.0	0.0	0.0	17.291192464913248	0.0
34	0.0	0.0	0.0	17.740409844010603	0.0
35	0.0	0.0	0.0	18.227396243820753	0.0
36	0.0	0.0	0.0	18.64686634111776	0.0
37	0.0	0.0	0.0	19.11179295891867	0.0
38	0.0	0.0	0.0	19.52858914324486	0.0
39	0.0	0.0	0.0	19.93903478426536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACCT	25	3.8838647E-5	45.000004	8
CAGCCGA	25	3.8838647E-5	45.000004	20
AAGACGG	30	2.160099E-6	45.000004	2
GTTACGG	25	3.8838647E-5	45.000004	2
GTTACAA	30	2.160099E-6	45.000004	9
AGCGCCC	25	3.8838647E-5	45.000004	15
CGGGATA	55	1.8189894E-12	45.000004	6
AACGAGC	25	3.8838647E-5	45.000004	15
AACGACG	25	3.8838647E-5	45.000004	1
CGCAGGA	25	3.8838647E-5	45.000004	4
AGTTATC	25	3.8838647E-5	45.000004	12
TGCACGG	45	3.8198777E-10	45.000004	2
AAGCTAC	55	1.8189894E-12	45.000004	8
AACCGAT	20	7.0249126E-4	45.0	19
CGTGCGT	20	7.0249126E-4	45.0	16
GGTGCGC	20	7.0249126E-4	45.0	9
TTGCACG	20	7.0249126E-4	45.0	1
ACTCAAT	20	7.0249126E-4	45.0	25
CGTTTTA	20	7.0249126E-4	45.0	1
ACGACAC	20	7.0249126E-4	45.0	44
>>END_MODULE