##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781277
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919887568685628	31.0	31.0	34.0	30.0	34.0
2	32.06258215716065	33.0	31.0	34.0	30.0	34.0
3	32.00543213226551	33.0	31.0	34.0	30.0	34.0
4	35.7183252546792	37.0	35.0	37.0	35.0	37.0
5	31.150937503599877	37.0	35.0	37.0	0.0	37.0
6	33.39415341805787	37.0	35.0	37.0	17.0	37.0
7	29.598762026784353	35.0	32.0	37.0	0.0	37.0
8	32.67208429276684	35.0	35.0	37.0	17.0	37.0
9	36.53556805076816	39.0	35.0	39.0	32.0	39.0
10	36.81404930645597	38.0	35.0	39.0	33.0	39.0
11	36.927674819558234	39.0	37.0	39.0	33.0	39.0
12	36.99697162466065	39.0	37.0	39.0	33.0	39.0
13	36.86667212781126	39.0	37.0	39.0	33.0	39.0
14	38.10659343613085	40.0	37.0	41.0	33.0	41.0
15	38.19501662022561	40.0	37.0	41.0	33.0	41.0
16	38.233041546084166	40.0	37.0	41.0	34.0	41.0
17	38.19574363509997	40.0	37.0	41.0	34.0	41.0
18	38.11957090762943	40.0	37.0	41.0	33.0	41.0
19	38.04968532287524	40.0	37.0	41.0	34.0	41.0
20	38.010109090629825	40.0	37.0	41.0	33.0	41.0
21	37.9368533823471	40.0	37.0	41.0	33.0	41.0
22	37.92702588198552	40.0	37.0	41.0	33.0	41.0
23	37.88424592046099	40.0	36.0	41.0	33.0	41.0
24	37.83643701273684	40.0	36.0	41.0	33.0	41.0
25	37.71822541812955	40.0	36.0	41.0	33.0	41.0
26	37.60749772487863	40.0	36.0	41.0	33.0	41.0
27	37.55743993487585	40.0	36.0	41.0	33.0	41.0
28	37.46538679623232	39.0	36.0	41.0	32.0	41.0
29	37.4698512819397	40.0	36.0	41.0	33.0	41.0
30	37.3696320255172	39.0	36.0	41.0	32.0	41.0
31	37.2456145515611	39.0	36.0	41.0	32.0	41.0
32	37.19738581834612	39.0	36.0	41.0	32.0	41.0
33	37.07903598851624	39.0	35.0	41.0	31.0	41.0
34	36.94795699860613	39.0	35.0	41.0	31.0	41.0
35	36.905479106642076	39.0	35.0	41.0	31.0	41.0
36	36.786412501583946	39.0	35.0	41.0	31.0	41.0
37	36.695309090117846	39.0	35.0	41.0	30.0	41.0
38	36.617384103205396	39.0	35.0	41.0	30.0	41.0
39	36.60656719703767	39.0	35.0	40.0	30.0	41.0
40	36.509737263480176	39.0	35.0	40.0	30.0	41.0
41	36.41829338378066	39.0	35.0	40.0	30.0	41.0
42	36.39062458001452	39.0	35.0	40.0	30.0	41.0
43	36.246207171080165	39.0	35.0	40.0	30.0	41.0
44	36.10238366162066	39.0	35.0	40.0	29.0	41.0
45	35.985711853798335	38.0	35.0	40.0	29.0	41.0
46	35.86919620057931	38.0	35.0	40.0	29.0	41.0
47	35.716892984178465	38.0	35.0	40.0	28.0	41.0
48	35.63515884891018	38.0	35.0	40.0	28.0	41.0
49	35.49816646336703	38.0	34.0	40.0	27.0	41.0
50	35.33217923988547	38.0	34.0	40.0	27.0	41.0
51	33.278995797905225	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	32.0
10	48.0
11	35.0
12	23.0
13	18.0
14	29.0
15	34.0
16	69.0
17	149.0
18	272.0
19	465.0
20	742.0
21	1205.0
22	1846.0
23	2668.0
24	3792.0
25	5214.0
26	6737.0
27	8063.0
28	9801.0
29	11906.0
30	15461.0
31	19996.0
32	26007.0
33	37341.0
34	53355.0
35	65574.0
36	77608.0
37	123871.0
38	156589.0
39	152293.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87900066173713	20.702644516605506	25.673864711235577	11.744490110421783
2	30.362342677437066	25.29077395085226	26.325362195482526	18.02152117622815
3	31.952559719536094	25.235479861815975	26.622055941746652	16.18990447690128
4	28.716191568419397	26.203766397833288	26.34827340367117	18.731768630076147
5	22.306301094234183	39.81046415035897	21.362333717746715	16.52090103766014
6	25.968638523852615	35.62705672891945	24.025025695111974	14.37927905211596
7	70.84580756889042	19.09988390801214	6.297510358042026	3.756798165055416
8	85.8171941577699	4.452710114338449	6.320293570654198	3.409802157237446
9	79.51558794128076	7.192071442010964	8.129255052945371	5.163085563762916
10	38.684103077397644	32.96615668962481	15.540966904183792	12.808773328793757
11	27.686723146848045	27.47373850759718	27.349198811689067	17.490339533865708
12	26.822625010079655	24.595757970604538	29.860856008816334	18.720761010499476
13	23.917253419721813	25.883137478768738	31.019343971472345	19.180265130037107
14	20.04180335527604	29.294987565229746	29.826809185474552	20.836399894019664
15	19.379938229334794	28.454440614532363	33.378558437020416	18.787062719112427
16	22.425720967083375	27.13403824763816	30.820694836786437	19.61954594849202
17	22.06720535738285	27.026393967824475	28.924824358070182	21.981576316722496
18	22.820203333772785	26.985563378929623	29.978995925900804	20.21523736139679
19	22.965222321916553	28.505254858392092	28.158130854997655	20.3713919646937
20	24.68714681220617	28.24081599739913	27.936698507699575	19.135338682695128
21	23.9753634114405	28.232880271657812	28.910232862352274	18.881523454549413
22	22.57957165000378	25.39777825278358	29.00008575703624	23.022564340176405
23	21.76820769074221	27.395533210372246	29.586945475164377	21.249313623721164
24	22.41970517498915	25.742086353495626	30.32688790275408	21.511320568761143
25	20.556217577120535	28.768285768043857	28.388522892648833	22.28697376218678
26	21.21040296847341	28.942999729929337	28.500519022062598	21.34607827953466
27	21.837965279919928	28.700064125783815	28.484903561732906	20.977067032563355
28	19.335523764298706	28.714271634772302	30.598878502758946	21.35132609817005
29	21.33327872188737	26.35646512056543	30.772440504456167	21.53781565309103
30	22.538997052261873	26.93385316603458	30.068592829431818	20.458556952271728
31	22.203520646326464	28.657953581124236	27.641156721623698	21.4973690509256
32	22.317308713810853	28.24798374968161	28.451368720697012	20.983338815810527
33	21.694866225423247	27.7119382754132	28.987158203812474	21.606037295351072
34	20.140743935889578	28.22699247514006	30.506721687698473	21.12554190127189
35	20.6757654455462	26.722788460430806	29.91218223498196	22.68926385904103
36	21.464218196619125	28.691872408889544	28.879513923998783	20.96439547049254
37	21.229858296097287	29.026068859060235	29.321098662830213	20.42297418201227
38	20.253764029915125	28.870682229222155	28.55683707571066	22.318716665152053
39	21.044136714635144	27.325263638888643	29.525379602880925	22.105220043595295
40	21.434907209606834	26.573801609416382	30.09777582086763	21.893515360109156
41	19.222375674696682	27.812542798520884	28.894617402022586	24.070464124759848
42	20.821424411572337	26.671078247535768	28.78441321067944	23.72308413021246
43	21.875723974979426	26.02495657750068	29.18350341812187	22.915816029398023
44	20.954795802257074	26.928093365093304	28.956183274306042	23.160927558343584
45	21.053224400564716	26.836960514644616	29.059731695672596	23.050083389118072
46	21.43605916979509	27.95039403438217	28.453544645497054	22.160002150325685
47	19.69544732534044	26.88713478062198	30.790231889585897	22.627186004451687
48	20.336961154622497	25.73082274276601	29.919349987264436	24.01286611534705
49	20.297410521492377	25.39790624836006	31.08679764027355	23.21788558987401
50	19.643353125715976	25.655945330529377	30.91899543951761	23.78170610423704
51	19.303012887874598	25.535501493068402	29.2994674104063	25.86201820865071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	408.0
1	806.0
2	1204.0
3	2077.5
4	2951.0
5	2259.5
6	1568.0
7	1318.5
8	1069.0
9	1099.0
10	1129.0
11	1182.0
12	1235.0
13	1249.5
14	1264.0
15	1321.5
16	1379.0
17	1441.0
18	1503.0
19	1961.0
20	2419.0
21	2796.0
22	3173.0
23	3734.0
24	4295.0
25	5548.5
26	7919.0
27	9036.0
28	11109.5
29	13183.0
30	15964.0
31	18745.0
32	20937.0
33	23129.0
34	25715.0
35	28301.0
36	30330.0
37	32359.0
38	35662.5
39	38966.0
40	43190.0
41	47414.0
42	53206.5
43	58999.0
44	66355.5
45	73712.0
46	74601.0
47	75490.0
48	71846.5
49	68203.0
50	61169.0
51	54135.0
52	48692.0
53	43249.0
54	39463.0
55	35677.0
56	33148.0
57	30619.0
58	28956.0
59	27293.0
60	25386.5
61	23480.0
62	21356.0
63	19232.0
64	15499.5
65	11767.0
66	9059.0
67	6351.0
68	5133.5
69	3916.0
70	3419.5
71	2923.0
72	2457.5
73	1992.0
74	1544.0
75	901.5
76	707.0
77	532.0
78	357.0
79	291.0
80	225.0
81	170.5
82	116.0
83	98.0
84	80.0
85	63.5
86	47.0
87	42.0
88	37.0
89	30.0
90	23.0
91	17.0
92	11.0
93	6.0
94	1.0
95	1.5
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	781277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.2769603984657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98788386310721	26.47778572469754
2	9.206819165351753	6.679908285146011
3	3.907540206415104	4.252610439705996
4	2.539952304179223	3.685669966108055
5	1.7114540145961148	3.1043174755649194
6	1.2675078063654075	2.758879829775841
7	0.9915732503809788	2.5179884475374075
8	0.7730420822345495	2.243489360285617
9	0.6335304203623169	2.0684302173636384
>10	5.883331659170916	42.39242892609373
>50	0.07492417395255244	1.8013784095892331
>100	0.019907386509887083	1.2257355221170663
>500	0.0018097624099896988	0.509202180478835
>1k	7.239049639958795E-4	0.2821752155361053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.1391311916260174	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	1062	0.13593130221419547	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	956	0.12236377110807051	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	875	0.11199612941376745	Illumina PCR Primer Index 2 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	829	0.10610833289601512	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12146780207276037	0.0
2	0.0	0.0	0.0	0.5369414433037194	0.0
3	0.0	0.0	0.0	0.7167752282481118	0.0
4	0.0	0.0	0.0	0.9618867571936713	0.0
5	0.0	0.0	0.0	1.8023057123145825	0.0
6	0.0	0.0	0.0	2.325423633359231	0.0
7	0.0	0.0	0.0	2.831774133885933	0.0
8	0.0	0.0	0.0	3.7320950187961506	0.0
9	0.0	0.0	0.0	4.066419464543305	0.0
10	0.0	0.0	0.0	4.973012132700694	0.0
11	0.0	0.0	0.0	5.955762168859445	0.0
12	0.0	0.0	0.0	6.861970850287414	0.0
13	0.0	0.0	0.0	7.179527875516622	0.0
14	0.0	0.0	0.0	7.309827372366011	0.0
15	0.0	0.0	0.0	7.56069870225285	0.0
16	0.0	0.0	0.0	8.081512702920987	0.0
17	0.0	0.0	0.0	8.770256899921538	0.0
18	0.0	0.0	0.0	9.483576247604883	0.0
19	0.0	0.0	0.0	9.938600521965961	0.0
20	0.0	0.0	0.0	10.349850309173314	0.0
21	0.0	0.0	0.0	10.895367456100717	0.0
22	0.0	0.0	0.0	11.495026731876147	0.0
23	0.0	0.0	0.0	12.097629905910452	0.0
24	0.0	0.0	0.0	12.547918343942033	0.0
25	0.0	0.0	0.0	12.948928485031557	0.0
26	0.0	0.0	0.0	13.328691360426584	0.0
27	0.0	0.0	0.0	13.702438443727385	0.0
28	0.0	0.0	0.0	14.093080943122606	0.0
29	0.0	0.0	0.0	14.510730509153603	0.0
30	0.0	0.0	0.0	14.991353898809257	0.0
31	0.0	0.0	0.0	15.427178836699404	0.0
32	0.0	0.0	0.0	15.839964570824431	0.0
33	0.0	0.0	0.0	16.24699050400818	0.0
34	1.279955764728771E-4	0.0	0.0	16.65171251681542	0.0
35	1.279955764728771E-4	0.0	0.0	17.090097366235025	0.0
36	1.279955764728771E-4	0.0	0.0	17.516066644736757	0.0
37	1.279955764728771E-4	0.0	0.0	17.915412843332135	0.0
38	1.279955764728771E-4	0.0	0.0	18.325638665927706	0.0
39	1.279955764728771E-4	0.0	0.0	18.745976139064634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAA	20	7.031898E-4	45.0	18
GTTCGGA	20	7.031898E-4	45.0	9
TGCGACG	20	7.031898E-4	45.0	1
GTATACG	20	7.031898E-4	45.0	1
ATGCACG	45	3.8380676E-10	45.0	1
ACGCGCT	20	7.031898E-4	45.0	21
CGTTATT	95	0.0	40.263157	1
GCGATGC	190	0.0	40.263157	9
TTATGCG	45	1.9268555E-8	40.0	1
ACGGGTA	85	0.0	39.705883	5
ACGGTCT	40	3.4563345E-7	39.375	30
ACGGGTC	110	0.0	38.86364	5
TCGACGT	35	6.24572E-6	38.571426	26
TACAACG	35	6.24572E-6	38.571426	1
TACGGGA	390	0.0	38.076923	4
ACAACGG	95	0.0	37.894737	2
GACGGGT	95	0.0	37.894737	4
AACGTTA	30	1.13958704E-4	37.500004	32
ATGCGGT	30	1.13958704E-4	37.500004	12
TCGAGCG	30	1.13958704E-4	37.500004	1
>>END_MODULE