FastQCFastQC Report
Sat 18 Jun 2016
SRR3551079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563144
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.2958390749080164No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG207.030216E-445.00000410
ACTGCGC207.030216E-445.00000439
TACGATG207.030216E-445.00000415
CTACGAT207.030216E-445.00000414
CAACTCG207.030216E-445.00000424
GGCGAAC207.030216E-445.00000410
TCGACGG406.8048394E-945.0000042
TAGTCGG207.030216E-445.0000042
TCACGAC253.8882623E-545.025
CTGCGTC253.8882623E-545.012
GGACGTA253.8882623E-545.08
GTTAACG302.1635224E-644.9999961
ATGGGCG1450.041.896555
TGGGCGA2250.041.06
ACGGGTC556.002665E-1140.9090925
TTAACGG556.002665E-1140.9090922
ACGGGAC2250.040.05
CGTTCGG403.4540244E-739.37500445
CGCCTTA403.4540244E-739.37500432
CACGGGC700.038.571434