##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563144
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.914185359339708	31.0	31.0	34.0	30.0	34.0
2	32.06284538235336	33.0	31.0	34.0	30.0	34.0
3	31.933899677524753	33.0	31.0	34.0	30.0	34.0
4	35.68910083388973	37.0	35.0	37.0	33.0	37.0
5	31.121102240279573	37.0	35.0	37.0	0.0	37.0
6	33.38865014987286	37.0	35.0	37.0	17.0	37.0
7	29.617227565240864	35.0	32.0	37.0	0.0	37.0
8	32.663006264827466	35.0	34.0	37.0	17.0	37.0
9	36.520806401204666	39.0	35.0	39.0	32.0	39.0
10	36.83692625687213	38.0	37.0	39.0	33.0	39.0
11	36.969281391615645	39.0	37.0	39.0	33.0	39.0
12	37.01014482974159	39.0	37.0	39.0	33.0	39.0
13	36.87338407227991	39.0	37.0	39.0	33.0	39.0
14	38.16454583552342	40.0	37.0	41.0	33.0	41.0
15	38.235184961572884	40.0	37.0	41.0	33.0	41.0
16	38.266752375946474	40.0	37.0	41.0	34.0	41.0
17	38.242252425667324	40.0	37.0	41.0	34.0	41.0
18	38.14740812296677	40.0	37.0	41.0	33.0	41.0
19	38.068488699160426	40.0	37.0	41.0	34.0	41.0
20	37.9821963831631	40.0	37.0	41.0	33.0	41.0
21	37.88709459747418	40.0	36.0	41.0	33.0	41.0
22	37.87505327234242	40.0	36.0	41.0	33.0	41.0
23	37.8226883354879	40.0	36.0	41.0	33.0	41.0
24	37.78556816728936	40.0	36.0	41.0	33.0	41.0
25	37.66013133408151	40.0	36.0	41.0	33.0	41.0
26	37.493170485701704	39.0	36.0	41.0	33.0	41.0
27	37.463456948844346	39.0	36.0	41.0	33.0	41.0
28	37.366923912889064	39.0	36.0	41.0	32.0	41.0
29	37.356047476311566	39.0	36.0	41.0	32.0	41.0
30	37.243774238915805	39.0	36.0	41.0	32.0	41.0
31	37.13162530365235	39.0	35.0	41.0	31.0	41.0
32	37.00975771738667	39.0	35.0	41.0	31.0	41.0
33	36.85154596337704	39.0	35.0	41.0	31.0	41.0
34	36.71886764308951	39.0	35.0	41.0	30.0	41.0
35	36.620986816870996	39.0	35.0	41.0	30.0	41.0
36	36.49205531800037	39.0	35.0	41.0	30.0	41.0
37	36.361319662466435	39.0	35.0	41.0	30.0	41.0
38	36.274412938786526	39.0	35.0	40.0	30.0	41.0
39	36.270953788018694	39.0	35.0	40.0	30.0	41.0
40	36.111598454391775	39.0	35.0	40.0	29.0	41.0
41	36.028129572542724	39.0	35.0	40.0	29.0	41.0
42	35.98164767803617	39.0	35.0	40.0	29.0	41.0
43	35.82004070006961	39.0	35.0	40.0	28.0	41.0
44	35.60175017402298	38.0	35.0	40.0	27.0	41.0
45	35.50491170997116	38.0	34.0	40.0	26.0	41.0
46	35.437918187888	38.0	34.0	40.0	26.0	41.0
47	35.30520080121603	38.0	34.0	40.0	26.0	41.0
48	35.231837682724134	38.0	34.0	40.0	26.0	41.0
49	35.065418436492266	38.0	34.0	40.0	26.0	41.0
50	34.912191197988435	38.0	34.0	40.0	24.0	41.0
51	32.99103604051539	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	23.0
10	24.0
11	25.0
12	13.0
13	19.0
14	22.0
15	32.0
16	61.0
17	124.0
18	264.0
19	392.0
20	753.0
21	1142.0
22	1736.0
23	2489.0
24	3500.0
25	4658.0
26	5892.0
27	7032.0
28	8089.0
29	9311.0
30	11224.0
31	14325.0
32	18847.0
33	26467.0
34	38377.0
35	47277.0
36	55580.0
37	87495.0
38	110020.0
39	107910.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89773130851079	20.88204793090222	25.591500575341296	10.628720185245692
2	33.47687270041055	24.472958958987398	24.87995965507934	17.17020868552271
3	32.31074112482775	24.505632662338588	26.799539726961484	16.384086485872174
4	28.66531473299902	26.55395422840339	26.287770090776068	18.492960947821516
5	21.918194990979217	40.02244541360647	21.758910687142187	16.30044890827213
6	25.76818717770233	36.01831858281363	24.491604278834544	13.721889960649497
7	70.11687951927038	19.53585583793843	6.75795178497862	3.589312857812567
8	84.9651243731621	5.00209537880187	7.040650348756268	2.992129899279758
9	78.97979912775418	8.070937451167019	8.500312531075533	4.4489508900032675
10	39.73104570056682	31.277435256346514	16.04935860099726	12.942160442089412
11	29.53631753157274	28.32028752858949	25.711008196837753	16.432386743000013
12	27.48977171025528	25.929424800761442	28.356690295910102	18.224113193073173
13	24.149240691546034	27.54464222294831	29.550701064026253	18.75541602147941
14	20.849196653076298	30.032993337405706	29.459427784012615	19.658382225505378
15	19.689280184109215	29.02934240620516	32.68950747943688	18.59186993024875
16	22.98186609464009	27.444667793672668	31.566704075689344	18.006762035997898
17	23.343940448624153	26.307658431946358	28.683604903896693	21.664796215532796
18	23.675471992953845	27.558137883028145	29.374902334038893	19.391487789979116
19	25.358878013438833	28.237005099938916	27.3752361740514	19.028880712570853
20	26.442259883795266	27.65438324833435	27.58814796925831	18.315208898612077
21	24.94512948730698	27.94063330160669	28.844842526955805	18.269394684130525
22	23.5703479039109	26.070951657124997	28.46287983180146	21.895820607162644
23	22.7066256588013	28.269501228815365	28.957957467361812	20.065915645021523
24	21.975373971843794	26.895785092267698	29.744789964911284	21.384050970977228
25	21.685217280127286	29.25379654226983	28.50549770573779	20.5554884718651
26	21.560382424388788	29.812090690835735	28.207527737132953	20.41999914764252
27	21.785546858352394	29.673937749492136	28.558414899208728	19.982100492946742
28	20.05224241046695	29.443623655761225	30.110770957339504	20.393362976432318
29	21.05340729902121	28.783579333172334	29.3457801201824	20.817233247624053
30	21.918017416504483	28.006335857258534	29.156308155640474	20.91933857059651
31	23.291555978577417	28.418486213117784	27.04352705524697	21.246430753057833
32	23.422250791982158	30.000142059579787	26.972852414302555	19.604754734135497
33	23.07686843862316	29.252553520946684	26.572954697200007	21.09762334323015
34	21.765658517182107	28.856207293338827	28.39344821218019	20.984685977298877
35	22.357514241472874	27.988223260835593	27.622419842882106	22.031842654809427
36	23.37288508800591	29.3452473967582	26.943730200446065	20.338137314789822
37	22.636661315755827	29.821857286946145	27.106565993777792	20.434915403520236
38	21.80721094427003	30.128350830338245	26.19489864048982	21.869539584901908
39	22.933033114088047	28.361484806727944	27.066966885911953	21.63851519327206
40	23.05502677823079	27.792358615203216	28.715213160399472	20.437401446166522
41	21.75979855951586	28.091571605131193	28.266304888270138	21.882324947082807
42	22.48909692725129	28.06511300839572	27.23797110508147	22.20781895927152
43	23.029278479394257	27.76643274189195	27.261055786796984	21.943232991916812
44	21.57405565894336	29.11688662224937	27.481248135468018	21.827809583339253
45	21.72019945165002	28.873076868438623	26.79421249271945	22.61251118719191
46	21.87451167019448	29.557626468540903	26.725668745471854	21.842193115792764
47	21.66728225817908	28.78606537581862	28.223864588808546	21.322787777193756
48	22.35396275197818	27.22216697683008	28.234341482817893	22.189528788373845
49	21.824790817268763	27.70605742048215	28.188527268336337	22.280624493912747
50	20.1451138607532	28.71325984117739	28.226883354879035	22.914742943190376
51	21.07489381046411	28.338577699487168	26.976936627221455	23.60959186282727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	313.0
1	655.5
2	998.0
3	2439.5
4	3881.0
5	3001.5
6	2122.0
7	1727.0
8	1332.0
9	1323.5
10	1315.0
11	1340.5
12	1366.0
13	1392.5
14	1419.0
15	1486.0
16	1553.0
17	1680.0
18	1807.0
19	1969.5
20	2132.0
21	2548.0
22	2964.0
23	3404.0
24	3844.0
25	4723.5
26	6694.0
27	7785.0
28	9237.5
29	10690.0
30	12681.5
31	14673.0
32	15862.5
33	17052.0
34	18848.0
35	20644.0
36	21857.5
37	23071.0
38	25464.0
39	27857.0
40	30106.5
41	32356.0
42	33825.5
43	35295.0
44	37016.0
45	38737.0
46	38964.5
47	39192.0
48	41074.0
49	42956.0
50	42000.5
51	41045.0
52	39083.5
53	37122.0
54	33890.0
55	30658.0
56	28186.5
57	25715.0
58	24195.0
59	22675.0
60	21535.5
61	20396.0
62	18334.0
63	16272.0
64	13233.5
65	10195.0
66	8129.0
67	6063.0
68	5111.0
69	4159.0
70	3504.0
71	2849.0
72	2391.5
73	1934.0
74	1682.5
75	1110.5
76	790.0
77	616.0
78	442.0
79	322.0
80	202.0
81	169.0
82	136.0
83	95.0
84	54.0
85	41.0
86	28.0
87	19.5
88	11.0
89	7.5
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.66673334316742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73143496193971	27.395555663773074
2	8.728011432217517	6.57511358466908
3	4.1880938395528275	4.732554416117956
4	2.7308763202315722	4.114527605893317
5	1.897478777234351	3.573591356320284
6	1.3872423747043598	3.135173316619887
7	1.069854929717459	2.820855824747846
8	0.825319774172725	2.486967988528575
9	0.643270766670845	2.180691759208103
>10	5.733304958671582	40.282363636925375
>50	0.046238860572472555	1.1439907543703192
>100	0.018401179206705975	1.2625307315208245
>500	0.0	0.0
>1k	4.718251078642558E-4	0.29608336130541857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1666	0.2958390749080164	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5514894946940746E-4	0.0	0.0	0.06588013012657508	0.0
2	3.5514894946940746E-4	0.0	0.0	0.2576605628400551	0.0
3	3.5514894946940746E-4	0.0	0.0	0.34911141732842754	0.0
4	3.5514894946940746E-4	0.0	0.0	0.48264742232892477	0.0
5	3.5514894946940746E-4	0.0	0.0	0.916461864105806	0.0
6	3.5514894946940746E-4	0.0	0.0	1.2247311522452518	0.0
7	3.5514894946940746E-4	0.0	0.0	1.4807935448126945	0.0
8	3.5514894946940746E-4	0.0	0.0	1.9343187532851278	0.0
9	3.5514894946940746E-4	0.0	0.0	2.119351355958689	0.0
10	3.5514894946940746E-4	0.0	0.0	2.670897674484679	0.0
11	3.5514894946940746E-4	0.0	0.0	3.355269700112227	0.0
12	3.5514894946940746E-4	0.0	0.0	3.943751509383035	0.0
13	3.5514894946940746E-4	0.0	0.0	4.179037688406518	0.0
14	3.5514894946940746E-4	0.0	0.0	4.277946670833748	0.0
15	3.5514894946940746E-4	0.0	0.0	4.466175614052534	0.0
16	3.5514894946940746E-4	0.0	0.0	4.870157544073985	0.0
17	3.5514894946940746E-4	0.0	0.0	5.3345147955052346	0.0
18	3.5514894946940746E-4	0.0	0.0	5.8842853692838775	0.0
19	3.5514894946940746E-4	0.0	0.0	6.185096529484466	0.0
20	3.5514894946940746E-4	0.0	0.0	6.5063287542795445	0.0
21	3.5514894946940746E-4	0.0	0.0	6.869113406162545	0.0
22	3.5514894946940746E-4	0.0	0.0	7.265814782719873	0.0
23	3.5514894946940746E-4	0.0	0.0	7.668731265892916	0.0
24	3.5514894946940746E-4	0.0	0.0	7.984813830920688	0.0
25	3.5514894946940746E-4	0.0	0.0	8.269288139445683	0.0
26	3.5514894946940746E-4	0.0	0.0	8.546126745557087	0.0
27	3.5514894946940746E-4	0.0	0.0	8.81426420240649	0.0
28	3.5514894946940746E-4	0.0	0.0	9.087728893497934	0.0
29	3.5514894946940746E-4	0.0	0.0	9.404166607475176	0.0
30	3.5514894946940746E-4	0.0	0.0	9.768904578580257	0.0
31	3.5514894946940746E-4	0.0	0.0	10.102211867657296	0.0
32	3.5514894946940746E-4	0.0	0.0	10.42894890116915	0.0
33	3.5514894946940746E-4	0.0	0.0	10.728872188996064	0.0
34	3.5514894946940746E-4	0.0	0.0	11.03749662608498	0.0
35	3.5514894946940746E-4	0.0	0.0	11.371691787535692	0.0
36	3.5514894946940746E-4	0.0	0.0	11.716541417470488	0.0
37	3.5514894946940746E-4	0.0	0.0	12.066718281647322	0.0
38	5.327234242041112E-4	0.0	0.0	12.395231059906525	0.0
39	5.327234242041112E-4	0.0	0.0	12.745407924083361	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	20	7.030216E-4	45.000004	10
ACTGCGC	20	7.030216E-4	45.000004	39
TACGATG	20	7.030216E-4	45.000004	15
CTACGAT	20	7.030216E-4	45.000004	14
CAACTCG	20	7.030216E-4	45.000004	24
GGCGAAC	20	7.030216E-4	45.000004	10
TCGACGG	40	6.8048394E-9	45.000004	2
TAGTCGG	20	7.030216E-4	45.000004	2
TCACGAC	25	3.8882623E-5	45.0	25
CTGCGTC	25	3.8882623E-5	45.0	12
GGACGTA	25	3.8882623E-5	45.0	8
GTTAACG	30	2.1635224E-6	44.999996	1
ATGGGCG	145	0.0	41.89655	5
TGGGCGA	225	0.0	41.0	6
ACGGGTC	55	6.002665E-11	40.909092	5
TTAACGG	55	6.002665E-11	40.909092	2
ACGGGAC	225	0.0	40.0	5
CGTTCGG	40	3.4540244E-7	39.375004	45
CGCCTTA	40	3.4540244E-7	39.375004	32
CACGGGC	70	0.0	38.57143	4
>>END_MODULE