FastQCFastQC Report
Sat 18 Jun 2016
SRR3551077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527244
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG6350.12043759625524424No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.10355736622891867No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC5380.10204004218160852TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.0298096E-445.040
CGGAATC207.0298096E-445.028
CGGGTAT453.8380676E-1045.06
CGAACGT207.0298096E-445.038
CGACGGT406.8030204E-945.028
GCGAATG406.8030204E-945.01
CGTTGAA207.0298096E-445.032
TACGGGT351.2102464E-745.04
CACTCGC207.0298096E-445.022
TGCGACG207.0298096E-445.01
TCGACCT207.0298096E-445.023
TATAACG253.8879232E-545.01
TACGTAG207.0298096E-445.01
TTTAACG302.1632586E-644.9999961
AAACGAG302.1632586E-644.99999637
TTAACGG603.6379788E-1241.2499962
CGGGTTA556.002665E-1140.9090926
AATGGGC1450.040.3448264
ACCGGGT451.9248546E-840.04
TACGAAT403.4534787E-739.37512